Effect of lactic acid bacterial inoculant with enzyme and rolled barley additive on the chemical composition and protein degradation of alfalfa silage

In the experiment, forage (dry matter 216.3 g.kg-1) from the 1st cut of alfalfa (Medicago, sativa L.) cv, Radius, mown at the early flowering stage, was ensiled. Alfalfa was ensiled without inoculants; with lactic acid bacteria and enzyme inoculants; with rolled barley; or with rolled barley and lactic acid bacteria and enzyme inoculants. Rolled barley was added in amounts of 50,100, 150 and 200 g.kg-1 of alfalfa forage, The bacterial acid enzyme inoculants (Bactozym) contained cultures of Lacto bacillus casein, Enterococcus faecium M74 and Pediococcus sp. (a total of 1 5 x 109 CPU in Ig of friable preparation) in the powdered part and the cellulase-hemicellulase complex (25000 n cat. in 1ml) and glucose-oxidase (4000 n cat. in 1ml) in the liquid part. The inoculants was used in a water solution in the amount of 10mg bacterial component and 0,2 ml enzymatic component / kg-1 alfalfa forage. The supplement of only rolled barley to alfalfa forage was found to have no significant effect on improving the processes of proteolysis and deamination in this type of silages, compared to alfalfa silages made with bacterial and enzyme inoculants. Significantly lower crude protein and WSC losses in the fermentation process, increased intensity of lactic acid fermentation, the lack of propionic and butyric acid fermentation, and significantly reduced processes of protein degradation were found in alfalfa-rolled barley silages made with the addition of lactic acid bacteria and enzyme inoculants.

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Evaluasi Kandungan Nutrisi Pakan dan Daya Hambat Tepung Biji Asam Kandis (Gracinia cowa) sebagai Bahan Pakan Unggas

Jurnal Ilmu dan Teknologi Peternakan Tropis ◽

10.33772/jitro.v5i3.4708 ◽

2018 ◽

Vol 5 (2) ◽

pp. 20

Author(s):

Febri Puska Padang ◽

Osfar Sjofjan ◽

Edhy Sudjarwo

Keyword(s):

Escherichia Coli ◽

Lactic Acid ◽

Lactic Acid Bacteria ◽

Crude Protein ◽

Dry Matter ◽

Laboratory Experiments ◽

Proximate Analysis ◽

Food Ingredient ◽

Feed Ingredients ◽

Inhibitory Bacteria

Kandis acid (Gracinia cowa) has been used for cooking, medicine, beauty, etc., but using of seeds has not been profitable so it was wasted. Seeds can be used as a fitobiotic to replace antibiotics or as feed ingredients. The purpose of this study was to determine the food ingredient and the inhibitory of bacteria on kandis seeds flour. This research was conducted by laboratory experiments using Kandis seeds flour as a material, analysis proximate used to represent the composition of feedstuft, bioactive used test flavonoid quantitaf seconder and the inhibitory test with hollow diffusion methode. The results of the test were dry matter 89.11%, crude fat 4.77, fiber 18.57, crude protein 0.99, tannin 0.29%, GE 5244 kcal / kg, Ca 0.72%, P 0, 22%, flavonoids 0.44%, density 413 g / mL. The smallest inhibitory bacteria produced by lactic acid bacteria then Salmonella and Escherichia coli. The food ingredient represented that value Kandis acid seeds flour can be used as food and there are bacterial inhibitory by the activity of flavonoids and tannins. The conclusion is the kandis acid seeds have potential as feed ingredients and phytobiotics Keywords: gracinia cowa, flavonoid, proximate analysis, inhibition

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Comparative characteristics during wilting for alfalfa conditioned by maceration or by a conventional roller-conditioner

Canadian Journal of Animal Science ◽

10.4141/a99-018 ◽

1999 ◽

Vol 79 (4) ◽

pp. 509-517 ◽

Cited By ~ 1

Author(s):

Suwarno Suwarno ◽

K. M. Wittenberg ◽

W. P. McCaughey

Keyword(s):

Lactic Acid ◽

Lactic Acid Bacteria ◽

Medicago Sativa ◽

Key Words ◽

Dry Matter ◽

Microbial Count ◽

Nutrient Profile ◽

Conditioning Treatment ◽

Acid Detergent Fibre ◽

Medicago Sativa L

A study was conducted to monitor nutrient and microbial count changes during wilting of alfalfa (Medicago sativa L.) in response to varying degrees of maceration at mowing. Early bloom alfalfa was mowed with either a roller-conditioner (CONV) or a macerator set to deliver four degrees of maceration during alfalfa mowing: LIGHT, LIGHT+, SEVERE, and SEVERE+. Macerated alfalfa reached 80% dry matter in 9–11 h compared with alfalfa mowed by roller-conditioner, which required 54 h. The most rapid wilting rates were associated with LIGHT+, SEVERE, and SEVERE+ maceration treatments when alfalfa was not exposed to precipitation. The wilting coefficient in the first 24 h was increased by more than 100% for the SEVERE treatment compared with alfalfa mowed using a conventional roller-conditioner in alfalfa that was not exposed to precipitation. Precipitation at 1.5 h post-mowing increased wilting time by 8.3 h to achieve 45% DM, and by 17.5 to achieve 80% DM in the SEVERE+ maceration treatment relative to alfalfa from the same conditioning treatment that was not exposed to precipitation. Precipitation at 24 h post-mowing increased wilting time to reach 80% DM by 11 h and 21 h for the LIGHT and LIGHT+ maceration treatments relative to alfalfa of the same mowing treatments not exposed to precipitation. Maceration of alfalfa resulted in a 24.2 to 26.8 h shorter wilting time relative to the conventional roller-conditioner treatment when alfalfa was exposed to precipitation at 24 h post-mowing. SEVERE and SEVERE+ maceration treatments at mowing resulted in higher (P < 0.05) neutral detergent fibre and acid detergent fibre levels post-wilting. Lactic acid bacteria populations on alfalfa tended to be higher with maceration within 1 h post-mowing (P < 0.10) and post-wilting (P < 0.08). Key words: Alfalfa, maceration, precipitation, wilting time, bacteria, nutrient profile, compressibility

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The effect of lactic acid additive on the quality and chemical composition of meadow grass silage

Biotechnology in Animal Husbandry ◽

10.2298/bah0304061p ◽

2003 ◽

Vol 19 (3-4) ◽

pp. 61-69

Author(s):

Jan Pyś ◽

Wladislaw Migdal ◽

Branislav Zivkovic ◽

Olga Kosovac ◽

Mihal Fabjan ◽

...

Keyword(s):

Lactic Acid ◽

Crude Protein ◽

Dry Matter ◽

Water Solution ◽

Water Soluble ◽

Soluble Carbohydrates ◽

Total N ◽

Grass Silage ◽

Fermentation Quality ◽

Dominant Grasses

In the experiment, forage from the 1st cut of meadow grasses mown at the heading stage of dominant grasses was ensiled. The control silages were made from fresh forage (dry matter 223.5 g?kg-1) and wilted forage (dry matter 351.9 g?kg-1). The experimental silages were made from fresh forages supplemented with a 5% water solution of lactic acid (0.5 and 0.7 l?100 kg-1 of forage) and 8% water solution of lactic acid (0.4 and 0.6 l?100 kg-1 of forage). The highest contents of crude protein, water-soluble carbohydrates and energy were found in silages supplemented with 5% lactic acid applied at 0.7 l?100 kg-1 of forage and in silages supplemented with 8% lactic acid applied at 0.6 l?100 kg-1 of forage. The above silage variants were also characterized by the lowest content of NH3-N in total-N, butyric acid and acetic acid, as well as the highest contents of lactic acid and the best indicators of fermentation quality. No important differences were found between silages made from wilted forage and silages made with lactic acid supplement.

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The effect of bacterial inoculants on kikuyu silage quality

The Journal of Agricultural Science ◽

10.1017/s0021859600065795 ◽

1994 ◽

Vol 122 (1) ◽

pp. 53-60 ◽

Cited By ~ 2

Author(s):

M. De Figueiredo ◽

J. P. Marais

Keyword(s):

South Africa ◽

Lactic Acid ◽

Lactic Acid Bacteria ◽

Acid Content ◽

Crude Protein ◽

Dry Matter ◽

Bacterial Inoculants ◽

Lactic Acid Content ◽

Kikuyu Grass ◽

Silage Quality

SUMMARYTwo commercial bacterial inoculants (Lacto-flora and Ecosyl) were added to kikuyu grass (Pennisetum clandestinum) at ensiling, in 1985 and 1988 respectively, at Cedara, South Africa, using laboratory silos. In 1985 (Expt 1), Lacto-flora was added on its own and in combination with the enzymes Celluclast or SP249. In 1988 (Expt 2), Ecosyl was added on its own and in combination with molasses, at two levels of addition.The addition of Lacto-flora alone did not significantly increase the lactic acid bacteria or the lactic acid content of the treated silage. However, treated silage contained 53·6% less iso-butyric acid and 53·7% less ammonia than the control silage. Kikuyu silage supplemented with a combination of Lacto-flora and Celluclast or SP249 had higher numbers of lactic acid bacteria at ensiling (0·231 × 108/ml) than the control silage or silage receiving Lacto-flora alone. However, only silage supplemented with the combination of Lacto-flora and Celluclast had a significantly higher lactic acid content (2·23 compared with 0·04, 0·18 and 0·13% DM for the control silage, silages with Lactoflora and with a combination of Lacto-flora +SP249, respectively). Nevertheless, this silage contained 19·78% more acetic than lactic acid.Silage supplemented with Ecosyl on its own did not differ significantly in digestibility, loss of dry matter, ammonia, total non-structural carbohydrates, lactic acid and crude protein, from the untreated silage. A significant decrease in silage pH (from 5·08 to 4·70) was observed when Ecosyl was added together with molasses at the higher level of addition only. However, no other benefit was obtained by the addition of Ecosyl in combination with molasses.

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EFFECT OF ADDITIVES CONTAINING LACTIC ACID BACTERIA AND/OR HYDROLYTIC ENZYMES WITH AN ANTIOXIDANT UPON THE PRESERVATION OF CORN OR ALFALFA SILAGE

Canadian Journal of Animal Science ◽

10.4141/cjas81-081 ◽

1981 ◽

Vol 61 (3) ◽

pp. 669-680 ◽

Cited By ~ 20

Author(s):

J. G. BUCHANAN-SMITH ◽

Y. T. YAO

Keyword(s):

Lactic Acid ◽

Lactic Acid Bacteria ◽

Crude Protein ◽

Dry Matter ◽

Hydrolytic Enzymes ◽

Water Soluble ◽

Soluble Carbohydrate ◽

Total N ◽

Alfalfa Silage ◽

Water Soluble Carbohydrate

A silage additive containing lactic acid bacteria was tested for its effect upon preservation of corn silage, 35–40% dry matter (DM), in four pairs of upright silos (100-t capacity). The additive did not affect (P > 0.05) final pH, lactic or acetic acid concentration, crude protein or NPN-N and NH4+-N expressed as a percent of total N. Recovery of energy from treated silage was greater than control in the two pairs of silos where this was determined, but recoveries of dry matter and crude protein were not consistently affected. Two silage additives, containing hydrolytic enzymes and an antioxidant with or without lactic acid bacteria, were tested on alfalfa, 20, 30, 36.5 and 47.3% DM, in 250-mL laboratory silos. The experimental design used a4 (DM level) × 4 (added glucose — 0,4,8 and 12%, DM basis) × 3 (additive — control, a hydrolytic enzyme/antioxidant additive (E/AO), and E/AO plus lactic acid bacteria (E/AO +)) factorial arrangement of treatments. Alfalfa was harvested using farm equipment. For 60-day silage, the additives as either a primary factor in the design or in second-order interactions with either silage DM or glucose showed no effect (P > 0.05) upon pH, lactic acid, acetic acid, butyric acid, Fleig score, residual water-soluble carbohydrate and percent of total N as NPN-N or ammonia-N. Poor quality silage resulted from the fermentation of alfalfa at 20 and 30% DM, and although additional glucose resolved this problem the additives did not. Beneficial effects of additional water-soluble carbohydrate in alfalfa silage fermentation are evident in data presented. Thus an additive containing an antioxidant and hydrolytic enzymes was not very effective and numbers of lactic acid bacteria in untreated alfalfa harvested with farm equipment must already be sufficient for adequate fermentation.

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EFFECT OF DIFFERENT ADDITIVES ON THE SILAGE QUALITY OF SWEET POTATO BRANCHES

Revista Caatinga ◽

10.1590/1983-21252019v32n223rc ◽

2019 ◽

Vol 32 (2) ◽

pp. 506-513

Author(s):

NERMY RIBEIRO VALADARES ◽

VALTER CARVALHO DE ANDRADE JUNIOR ◽

ROSANA CRISTINA PEREIRA ◽

CÍNTIA MARIA TEIXEIRA FIALHO ◽

MARCOS AURÉLIO MIRANDA FERREIRA

Keyword(s):

Lactic Acid ◽

Lactic Acid Bacteria ◽

Sweet Potato ◽

Crude Protein ◽

Dry Matter ◽

Neutral Detergent Fiber ◽

Mineral Matter ◽

Citrus Pulp ◽

Silage Quality

ABSTRACT Sweet potato branches have potential use in animal feeding, even fresh or ensiled, and some additives may enhance their quality. This study aimed at assessing silage quality of sweet potato branches using different additives. We ensiled sweet potato branches without additive (control) and with the addition of 10% disintegrated corn with straw and corncob (DCSC), 10% sugarcane bagasse from alembic, 10% citrus pulp, and 10% coffee husk. Three samples of each plot were obtained after drying for measurements of dry matter, crude protein, neutral detergent fiber (NDF), acid detergent fiber (ADF), cellulose, hemicellulose, lignin, mineral matter, starch, soluble sugars, pH, lactic acid bacteria population, gas, and effluent losses. Silage with DCSC, citrus pulp, and coffee rusk reached the highest dry matter contents. Moreover, citrus pulp silage had a high content of NDF. In contrast, DCSC silage showed the lowest content of ADF. Lignin was low in silage with DCSC, citrus pulp, and control (without additive). Suitable amounts of starch, crude protein, and lactic acid bacteria population were found in all treatments. Silage with DCSC achieved the best results for the assessed variables. Therefore, DCSC is the most suitable additive to improve silage quality of sweet potato branches.

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Effects ofStreptococcus bovisIsolated from Bovine Rumen on the Fermentation Characteristics and Nutritive Value of Tanzania Grass Silage

The Scientific World JOURNAL ◽

10.1155/2016/8517698 ◽

2016 ◽

Vol 2016 ◽

pp. 1-6 ◽

Cited By ~ 3

Author(s):

Anderson de Moura Zanine ◽

Emerson Alencar Bonelli ◽

Alexandre Lima de Souza ◽

Daniele de Jesus Ferreira ◽

Edson Mauro Santos ◽

...

Keyword(s):

Lactic Acid ◽

Lactic Acid Bacteria ◽

Crude Protein ◽

Dry Matter ◽

Nutritive Value ◽

Protein Recovery ◽

Ammoniacal Nitrogen ◽

Streptococcus Bovis ◽

Grass Silage ◽

Colony Forming Units

This study aimed to evaluate the effects ofStreptococcus bovison the fermentation characteristics and nutritive value of Tanzania grass silage. Tanzania grass was chopped and left untreated (U) or treated withStreptococcus bovisJB1 at 1 × 106colony-forming units per gram (cfu/g) of fresh forage orStreptococcus bovisHC5 at 1 × 106 cfu/g of fresh forage and packed into sixtuplicate laboratory silos. The largest number of enterobacteria, molds and yeast (M&Y) occurred in untreated silages and the smallest populations of enterobacteria and M&Y and the largest numbers of lactic acid bacteria (LAB), at 9.81 and 9.87 log cfu/g, were observed inStreptococcus bovisJB1 and HC5, respectively (P<0.05). Silages treated with JB1 and HC5 had lower (P<0.05) silage pHs and concentrations of ammoniacal nitrogen (NH3-N) than untreated silages. The application ofStreptococcus bovisJB1 and HC5 resulted in fewer losses through gases and effluents (P<0.05), which resulted in greater dry matter recovery (DMR) and crude protein recovery (CPR) (P<0.05).Streptococcus bovisJB1 and HC5 improved the fermentative profile and increased the concentration of crude protein and DMR and CPR in Tanzania grass silage.

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Improving ensiling characteristics by adding lactic acid bacteria modifies in vitro digestibility and methane production of forage-sorghum mixture silage

Scientific Reports ◽

10.1038/s41598-021-81505-z ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Chatchai Kaewpila ◽

Pongsatorn Gunun ◽

Piyawit Kesorn ◽

Sayan Subepang ◽

Suwit Thip-uten ◽

...

Keyword(s):

Lactic Acid ◽

Lactic Acid Bacteria ◽

Nutritive Value ◽

Gas Production ◽

In Vitro Digestibility ◽

Acid Fermentation ◽

Design Factor ◽

Forage Sorghum ◽

Randomized Design

AbstractImproving the nutrition of livestock is an important aspect of global food production sustainability. This study verified whether lactic acid bacteria (LAB) inoculant could promote ensiling characteristics, nutritive value, and in vitro enteric methane (CH4) mitigation of forage sorghum (FS) mixture silage in attacking malnutrition in Zebu beef cattle. The FS at the soft dough stage, Cavalcade hay (CH), and cassava chip (CC) were obtained. The treatments were designed as a 4 × 2 factorial arrangement in a completely randomized design. Factor A was FS prepared without or with CH, CC, and CH + CC. Factor B was untreated or treated with Lactobacillus casei TH14. The results showed that all FS mixture silages preserved well with lower pH values below 4.0 and higher lactic acid contents above 56.4 g/kg dry matter (DM). Adding LAB boosted the lactic acid content of silages. After 24 h and 48 h of in vitro rumen incubation, the CC-treated silage increased in vitro DM digestibility (IVDMD) with increased total gas production and CH4 production. The LAB-treated silage increased IVDMD but decreased CH4 production. Thus, the addition of L. casei TH14 inoculant could improve lactic acid fermentation, in vitro digestibility, and CH4 mitigation in the FS mixture silages.

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Microbiological and Chemical Properties of Chokeberry Juice Fermented by Novel Lactic Acid Bacteria with Potential Probiotic Properties during Fermentation at 4 °C for 4 Weeks

Foods ◽

10.3390/foods10040768 ◽

2021 ◽

Vol 10 (4) ◽

pp. 768

Author(s):

Christos Bontsidis ◽

Athanasios Mallouchos ◽

Antonia Terpou ◽

Anastasios Nikolaou ◽

Georgia Batra ◽

...

Keyword(s):

Lactic Acid ◽

Lactic Acid Bacteria ◽

Chemical Properties ◽

Storage Period ◽

Probiotic Strain ◽

Physicochemical Characteristics ◽

Probiotic Properties ◽

Acid Fermentation ◽

Chokeberry Juice ◽

Fermented Juice

On the frame of this research survey, a novel potentially probiotic strain (Lactobacillus paracasei SP5) recently isolated from kefir grains was evaluated for chokeberry juice fermentation. Chokeberry juice was retrieved from the variety Aronia melanocarpa, a plant known to provide small, dark berries and to be one of the richest sources of antioxidants. The juice was subsequently fermented inoculating L. paracasei SP5 for 48 h at 30 °C. The fermented juices were left at 4 °C and tested regarding microbiological and physicochemical characteristics for 4 weeks. The potentially probiotic strain was proved capable of performing lactic acid fermentation at 30 °C. Cell viability of L. paracasei was detected in high levels during fermentation and the whole storage period, while the fermented juice showed higher levels of viability in juice with 40.3 g/L of initial sugar concentration. No ethanol was detected in the final fermented juice. Fermented chokeberry juice was characterized by aromatic desirable volatiles, which were retained in adequate levels for the whole storage period. Specifically, the occurrence of organic esters detected in fermented juices is considered as positive evidence of the provision of fruity and floral notes to the final product. During storage, total phenolics content and antioxidant activity were observed in higher levels in fermented chokeberry juice compared with non-fermented juice. Subsequently, fermentation of chokeberry juice by potentially probiotic lactic acid bacteria could provide high industrialization potential, providing the market with a nutritional beverage of good volatile quality with an enhanced shelf-life compared with an unfermented fresh juice.

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