scholarly journals The effects of probiotics on Campylobacter jejuni and Salmonella spp. with respect to the meat and the organs of slaughtered chickens

2010 ◽  
Vol 26 (5-6) ◽  
pp. 393-402 ◽  
Author(s):  
S. Ivanovic ◽  
M.Z. Baltic ◽  
N. Karabasil ◽  
S. Lilic
Keyword(s):  
Campylobacter Jejuni ◽  
Bacteriological Examination ◽  
Ambient Conditions ◽  
Perianal Region ◽  
Salmonella Spp ◽  
Selective Media ◽  
Campylobacter Spp

Our research deals with the effects of probiotics on Campylobacter jejuni and Salmonella spp. with respect to the meat and organs of slaughtered chickens. For the scope of our experiment, we used 250 one-day old chicks, divided into 5 groups. Initially, control chicken group was fed with feed not containing probiotics. Other groups were fed with feed containing different probiotics. Fattening-intended food was standardized for all groups. All chicken groups were exposed to the same ambient conditions. Following 42 days period of fattening, chickens were slaughtered. We took 30 samples of liver, intestine and swabs from perianal region for the needs of bacteriological examination. Campylobacter spp. and Salmonella spp. were determined by selective media. On the basis of obtained results, we can say that the application of probiotics in chicken feed reduces considerably the onset of Campylobacter jejuni and Salmonella spp. in meat and organs.

Comparison of Three Selective Media and Validation of the VIDAS Campylobacter Assay for the Detection of Campylobacter jejuni in Ground Beef and Fresh-Cut Vegetables

2011 ◽  
Vol 74 (3) ◽  
pp. 456-460 ◽  
Author(s):  
JUNG-WHAN CHON ◽  
JI-YEON HYEON ◽  
IN-SOO CHOI ◽  
CHAN-KYU PARK ◽  
SOO-KI KIM ◽  
...  
Keyword(s):  
Campylobacter Jejuni ◽  
Ground Beef ◽  
Food Samples ◽  
Assay Method ◽  
Enzyme Linked Immunosorbent Assay ◽  
Culture Methods ◽  
Selective Media ◽  
Fresh Cut ◽  
Microaerobic Conditions ◽  
Campylobacter Spp

In this study, three different selective media, modified cefoperazone charcoal deoxycholate agar (mCCDA), Karmali agar, and Preston agar, were compared for isolating Campylobacter jejuni from artificially contaminated ground beef and fresh-cut vegetables that have different levels of background microflora. Concurrently, an automated enzyme-linked immunosorbent assay method for detecting Campylobacter spp. (VIDAS Campylobacter) was evaluated by comparing it with the culture methods. Food samples inoculated with C. jejuni were enriched in Bolton broth at 42°C for 44 h and then streaked onto the three different selective media, followed by incubation under microaerobic conditions at 42°C for 48 h. The enriched Bolton broth (1 ml) was used in the VIDAS Campylobacter assay. No statistical differences in sensitivities were observed between the three selective media for ground beef and fresh-cut vegetables, but the selectivity of Preston agar was better (P < 0.05) than those of mCCDA and Karmali agar. The VIDAS Campylobacter assay showed a recovery rate similar (P > 0.05) to those of all of the medium combinations in ground beef. However, more positive samples (P < 0.05) were detected with the VIDAS Campylobacter than with the selective agars, except for the combinations of mCCDA plus Preston agar or mCCDA plus Karmali agar plus Preston agar in fresh-cut vegetables.

Prevalence and Antimicrobial Resistance of Campylobacter spp. Isolated from Poultry Carcasses in Poland

2013 ◽  
Vol 76 (8) ◽  
pp. 1451-1455 ◽  
Author(s):  
KINGA WIECZOREK ◽  
IWONA KANIA ◽  
JACEK OSEK
Keyword(s):  
Campylobacter Jejuni ◽  
23S Rrna ◽  
Rrna Gene ◽  
Campylobacter Coli ◽  
Genetic Determinants ◽  
Gyra Gene ◽  
23S Rrna Gene ◽  
Pcr Method ◽  
Almost All ◽  
Campylobacter Spp

The purpose of the present study was to determine the prevalence of Campylobacter in poultry carcasses at slaughter in Poland. For the isolated strains, resistance to selected antibiotics and the associated genetic determinants were identified. A total of 498 Campylobacter isolates were obtained from 802 poultry samples during the 2-year study period. Strains were identified to species with the PCR method; 53.6% of the strains were Campylobacter jejuni and 46.4% were Campylobacter coli. A high percentage of the tested Campylobacter strains were resistant to ciprofloxacin and nalidixic acid (74.1 and 73.5%, respectively) followed by tetracycline (47.4%) and streptomycin (20.5%). Only one C. jejuni and two C. coli isolates were resistant to gentamicin. Seventy-nine (15.9%) of the 498 strains were resistant to three or more classes of antibiotics examined. Higher levels of resistance, irrespective of the antimicrobial agent tested, were found within the C. coli group. Almost all strains resistant to quinolones (99.5%) and to tetracycline (99.6%) carried the Thr-86-to-Ile mutation in the gyrA gene and possessed the tet(O) marker, respectively. All isolates resistant to erythromycin had the A2075G mutation in the 23S rRNA gene. These results reveal that poultry carcasses in Poland are a reservoir of potentially pathogenic and antimicrobial-resistant Campylobacter strains for humans, which may pose a public health risk.

scholarly journals Campylobacter jejuni, Campylobacter coli, Salmonella spp. e Yersinia enterocolitica isoladas de animais silvestres em um centro de reabilitação

2018 ◽  
Vol 38 (9) ◽  
pp. 1838-1843
Author(s):  
Débora R. Silveira ◽  
Camile Milan ◽  
Marina M. Ferrasso ◽  
Priscila A. Dias ◽  
Thamíris P. Moraes ◽  
...  
Keyword(s):  
Campylobacter Jejuni ◽  
Yersinia Enterocolitica ◽  
Salmonella Enterica ◽  
Campylobacter Coli ◽  
Salmonella Spp ◽  
Didelphis Albiventris

RESUMO: Muitas espécies de animais silvestres de vida livre servem como reservatório de bactérias patogênicas que ameaçam a saúde humana e dos animais domésticos. Algumas bactérias, como Campylobacter jejuni, Campylobacter coli, Yersinia enterocolitica e Salmonella enterica, causam enfermidades em humanos e podem contaminar os animais domésticos e silvestres. O Núcleo de Reabilitação da Fauna Silvestre da Universidade Federal de Pelotas (NURFS-UFPel) soluciona uma demanda regional específica de atenção à fauna silvestre brasileira. O objetivo desse trabalho foi identificar a presença de Campylobacter jejuni, Campylobacter coli, Salmonella spp. e Yersinia enterocolitica em animais silvestres que se encontravam em processo de reabilitação. Foram coletadas amostras de fezes, com uso de zaragatoas estéreis, de 34 aves, 16 mamíferos e 23 répteis. Dos 73 animais amostrados, quatro (5,48%) albergavam Y. enterocolitica, sendo duas aves, um mamífero e um réptil. Salmonella e Campylobacter não foram isolados. Os perfis de bandas dos isolados de Y. enterocolitica analisados pela rep-PCR foram diferentes entre si. Esses resultados indicam que as cepas isoladas não estão relacionadas entre si, não possuindo uma origem comum recente. Vanellus chilensis, Turdus rufiventris, Didelphis albiventris e Pantherophis guttatus podem albergar Y. enterocolitica e eliminá-la nas fezes, oferecendo risco de disseminação desse micro-organismo no ambiente, além de constituírem possíveis fontes de contaminação para humanos e outros animais.

Incidence of Salmonella, Campylobacter jejuni, Campylobacter coli, and Listeria monocytogenes in Poultry Carcasses and Different Types of Poultry Products for Sale on the Belgian Retail Market

1999 ◽  
Vol 62 (7) ◽  
pp. 735-740 ◽  
Author(s):  
M. UYTTENDAELE ◽  
P. DE TROY ◽  
J. DEBEVERE
Keyword(s):  
Listeria Monocytogenes ◽  
Campylobacter Jejuni ◽  
Broiler Chickens ◽  
Salmonella Enteritidis ◽  
Contamination Level ◽  
Contamination Rate ◽  
Campylobacter Coli ◽  
Retail Market ◽  
Salmonella Spp ◽  
Poultry Products

From January 1997 to May 1998, 772 samples of poultry carcasses and poultry products for sale on the retail market in Belgium were analyzed for the presence of Salmonella spp., Salmonella Enteritidis, Campylobacter jejuni, C. coli, and Listeria monocytogenes per 100 cm2 or 25 g. Poultry samples were contaminated with Salmonella (36.5%), C. jejuni and C. coli (28.5%), and L. monocytogenes (38.2%). In about 12.3% of the poultry samples, the L. monocytogenes contamination level exceeded 1 CFU per g or cm2. Significant differences in pathogen contamination rates of poultry products were noticed between the poultry products originating from Belgian, French, and U.K. abattoirs. Poultry products derived from broiler chickens running free in pine woods until slaughtering age (12 to 13 weeks) had a significantly (P < 0.05) lower contamination rate of Salmonella than poultry products from enclosed broilers slaughtered at the age of 6 to 8 weeks. A significantly (P < 0.05) lower pathogen contamination rate was noted for Salmonella, C. jejuni, and C. coli for poultry cuts without skin compared to poultry cuts with skin on. An increase in pathogen contamination rate was noticed during cutting and further processing. To diminish C. jejuni, C. coli, Salmonella, and L. monocytogenes contamination rates, hygienic rules of slaughter and meat processing must be rigorously observed. At the moment, zero tolerance for these pathogens is not feasible, and there is a need to establish criteria allowing these pathogens to be present at reasonable levels in the examined poultry samples.

scholarly journals Prevalence of Pathogens in Poultry Meat: A Meta-Analysis of European Published Surveys

Foods ◽  
2018 ◽  
Vol 7 (5) ◽  
pp. 69 ◽  
Author(s):  
Andiara Gonçalves-Tenório ◽  
Beatriz Silva ◽  
Vânia Rodrigues ◽  
Vasco Cadavez ◽  
Ursula Gonzales-Barron
Keyword(s):  
Meta Analysis ◽  
Management Strategies ◽  
Poultry Meat ◽  
Salmonella Spp ◽  
Cold Preservation ◽  
Incidence Data ◽  
Pooled Prevalence ◽  
Risk Management Strategies ◽  
Study Characteristics ◽  
Campylobacter Spp

The objective of this study was to investigate and summarize the levels of incidence of Salmonella spp., Listeria monocytogenes, Staphylococcus aureus and Campylobacter spp. in poultry meat commercialized in Europe. After systematic review, incidence data and study characteristics were extracted from 78 studies conducted in 21 European countries. Pooled prevalence values from 203 extracted observations were estimated from random-effects meta-analysis models adjusted by pathogen, poultry type, sampling stage, cold preservation type, meat cutting type and packaging status. The results suggest that S. aureus is the main pathogen detected in poultry meat (38.5%; 95% CI: 25.4–53.4), followed by Campylobacter spp. (33.3%; 95% CI: 22.3–46.4%), while L. monocytogenes and Salmonella spp. present lower prevalence (19.3%; 95% CI: 14.4–25.3% and 7.10%; 95% CI: 4.60–10.8%, respectively). Despite the differences in prevalence, all pathogens were found in chicken and other poultry meats, at both end-processing step and retail level, in packed and unpacked products and in several meat cutting types. Prevalence data on cold preservation products also revealed that chilling and freezing can reduce the proliferation of pathogens but might not be able to inactivate them. The results of this meta-analysis highlight that further risk management strategies are needed to reduce pathogen incidence in poultry meat throughout the entire food chain across Europe, in particular for S. aureus and Campylobacter spp.

scholarly journals Consumption of minas frescal cheese may be a source of human infection by Campylobacter jejuni

2020 ◽  
Vol 36 (2) ◽  
Author(s):  
Guilherme Paz Monteiro ◽  
Roberta Torres de Melo ◽  
Eliane Pereira Mendonça ◽  
Priscila Christen Nalevaiko ◽  
Mariela Moura Carreon ◽  
...  
Keyword(s):  
Campylobacter Jejuni ◽  
Low Temperatures ◽  
Human Infection ◽  
Emerging Pathogen ◽  
Dairy Food ◽  
Source Of Infection ◽  
Fresh Cheese ◽  
Sources Of Infection ◽  
Metabolic Activities ◽  
Campylobacter Spp

Campylobacter spp. is an emerging pathogen that causes gastroenteritis in humans and the consumption of dairy food can characterize sources of infection. We aimed to verify the viability and a presence of transcripts associated with characteristics of virulence and adaptation of C. jejuni isolated from Minas Frescal cheeses, produced with contaminated milk and stored under refrigeration for up to ten days. The samples were analyzed for bioindicators, Campylobacter spp., pH, acidity, moisture and sodium chloride. Campylobacter spp. recovered were evaluated for the production of transcripts of: ciaB, dnaJ, p19 and sodB. The results were correlated with the viability of C. jejuni and changes in their transcriptome. Storage at low temperatures reduced C. jejuni from the first to the fourth day. The variations in humidity, pH and acidity influenced the decreasing of C. jejuni. There was a reduction in transcripts' production of the four genes, more pronounced on the fourth day, indicating the inability of the microorganism to perform its metabolic activities, due to the conditions of injury. Despite the presence of mechanisms of virulence and adaptation, C. jejuni could not remain viable four days after production. However, consumption of fresh cheese contaminated with Campylobacter jejuni can be a source of infection when consumed up to four days after production.

scholarly journals Occurrence and antimicrobial susceptibility of Salmonella spp. isolated from domestic pigeons Columba livia var. domestica in 2007-2017 in Poland

2019 ◽  
Vol 75 (05) ◽  
pp. 6280-2019
Author(s):  
ALEKSANDRA LEDWOŃ ◽  
MAGDALENA RZEWUSKA ◽  
MICHAŁ CZOPOWICZ ◽  
MAGDALENA KIZERWETTER-ŚWIDA ◽  
DOROTA CHROBAK-CHMIEL ◽  
...  
Keyword(s):  
Antimicrobial Susceptibility ◽  
Columba Livia ◽  
Diffusion Method ◽  
Carrier Status ◽  
Salmonella Spp ◽  
Internal Organs ◽  
Selective Media ◽  
Bacterial Diseases ◽  
Domestic Pigeons

Salmonellosis is one of the most important bacterial diseases in pigeons. The disease occurs in birds of different ages, and long-term Salmonella spp. carrier status is a major problem. The study included 499 samples from live pigeons and internal organs from 265 necropsied birds. The samples were cultured on a standard and selective media. Their antimicrobial susceptibility was carried out by the Kirby-Bauer diffusion method. Thirty one Salmonella spp. isolates were cultured. Most isolates were obtained from the internal organs of dead or euthanised pigeons, and only single isolates were obtained from droppings and cloacal swabs. A total of 4% of the samples tested were positive. The greatest percentage of isolates was susceptible to enrofloxacin (96.3%) and florfenicol (95.7%). Sulfamethoxazole and trimethoprim combination was effective against 73.9% of the isolates. Despite the fact of increasing antibiotic resistance of the investigated bacteria, it is comforting that enrofloxacin remains highly effective despite its widespread use in birds.

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