scholarly journals Polyphenol content and antioxidant activity of sour cherries from Serbia

2012 ◽  
Vol 18 (1) ◽  
pp. 53-62 ◽  
Author(s):  
Milan Mitic ◽  
Mirjana Obradovic ◽  
Danijela Kostic ◽  
Ruzica Micic ◽  
Emilija Pecev
Keyword(s):  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Sour Cherry ◽  
Total Phenols ◽  
Climate Conditions ◽  
Growing Seasons ◽  
Significant Difference ◽  
Neochlorogenic Acid ◽  
Total Antioxidant ◽  
Total Anthocyanins

The aim of this study is to evaluate the content of phenolics: the total phenols (TP), flavonoids (TF), anthocyanins (TA), as well as the total antioxidant capacity (TAC) in three sour cherry cultivars (Prunus cerasus L.) introduced to the southeast Serbia climate conditions. Among the researched sour cherries, ?Oblacinska? cultivar contained the highest amounts of all groups of phenolics, followed by ?Cigancica? > ?Marela?. A significant difference were observed in the phenolic content among different cultivars and growing seasons (p<0.05), and the phenolic compounds were significantly higher in the growing season 2009. The examined cultivars possess a high antioxidant capacity, and all phenolics of highy correlation with TAC. The following compounds were identified and quantified using HPLC-DAD: 4 anthocyanins, the most abundant of which was cyaniding-3-glucoside in ?Marela? and ?Oblacinska?, and cyanidin-3-glucosylrutinoside in ?Cigancica?, and 4 hydroxycinnamic acids, the most abundant of which was neochlorogenic acid in all sour cherry cultivars. The growing and ripening process on the tree of sour cherry cv. Oblacinska was evaluated, also. The results showed significant increases in total phenols during the ripening, the total anthocyanins and total antioxidant capacity and 4 quantified anthocyanins, however the neochlorogenic acid decreased during the ripening. The study indicated that the growing and climate conditions in southeast Serbia are convenient for introducing sour cherry cultivars.

scholarly journals Pengaruh aplikasi gel teh hijau (Camellia sinensis) terhadap kadar total antioxidant capacity (TAOC) sebagai perawatan tambahan dari skeling dan root planing pada pasien dengan periodontitis kronisEffect of green tea (Camellia sinensis) gel application on the total antioxidant capacity (TAOC) levels as adjunct to scaling and root planing in patients with chronic periodontitis

2020 ◽  
Vol 32 (2) ◽  
pp. 156
Author(s):  
Nita Nurniza ◽  
Ina Hendiani ◽  
Ira Komara
Keyword(s):  
Antioxidant Capacity ◽  
Green Tea ◽  
Camellia Sinensis ◽  
Chronic Periodontitis ◽  
Total Antioxidant Capacity ◽  
Scaling And Root Planing ◽  
Root Planing ◽  
Significant Difference ◽  
Total Antioxidant ◽  
Test Side

Pendahuluan: Mekanisme peradangan periodontitis kronis menghasilkan reaksi oksidasi, diketahui melalui kadar total antioxidant capacity (TAOC) cairan sulkus gingiva. Teh hijau dapat menghambat bakteri patogen periodontal sehingga kerusakan jaringan periodontal tidak bertambah parah. Tujuan penelitian adalah menganalisis kadar TAOC sebelum dan sesudah skeling dan root planing disertai gel teh hijau sebagai antioksidan. Metode: Jenis penelitian eksperimen semu, rumus ukuran sampel untuk menguji perbedaan dua rata-rata data tidak berpasangan, setiap subyek memiliki poket ≥ 5 mm, dikelompokkan sisi uji (n=14) dan sisi kontrol (n=14) (split mouth). Pengambilan cairan sulkus gingiva dilakukan pada hari ke-0, 15, dan 30, menggunakan metode absorbing paper strip dengan cara intracrevicular. Kedua sisi dilakukan skeling dan root planing, sisi uji diberikan gel. Hasil: Perbandingan kadar TAOC antara sisi kontrol dan sisi uji pada hari ke-0 (p=0,986), hari ke-15 (p=0,836), dan hari ke-30 (p=0,371) menunjukkan tidak terdapat perbedaan bermakna (p-value>0,05). Perbandingan rerata selisih kadar TAOC dalam kedua kelompok antara hari ke-0 dengan ke-15 (p=0,946), hari ke-0 dengan hari ke-30 (p=0,504), serta hari ke-15 dengan hari ke-30 (p=0,811) jugwa menunjukkan tidak terdapat perbedaan bermakna. Simpulan: Tidak terdapat pengaruh aplikasi gel teh hijau (Camellia sinensis) terhadap kadar total antioxidant capacity (TAOC) pada perawatan periodontitis kronis berupa skeling dan root planing.Kata kunci: Gel teh hijau, periodontitis kronis, skeling dan root planing, total antioxidant capacity (TAOC). ABSTRACTIntroduction: The inflammatory mechanism of chronic periodontitis produces an oxidation reaction, measured through the level of total antioxidant capacity (TAOC) of the gingival crevicular fluid. Green tea can inhibit periodontal pathogenic bacteria so that periodontal tissue damage will not worsen. This study was aimed to analyse the levels of TAOC before and after scaling and root planing with green tea gel as an antioxidant. Methods: The research was quasi-experimental. The sample size formula was used to test the difference between two unpaired data means. Each subject has a pocket ≥ 5 mm, grouped by the test side (n = 14) and the control side (n = 14) (split-mouth). The gingival crevicular fluid was collected on days 0, 15, and 30, using the intracrevicular method with absorbing paper strips. Both sides were treated with scaling and root planing, and the test side was administered with the green tea gel. Results: Comparison of TAOC levels between the control side and the test side on day 0 (p = 0.986), day 15 (p = 0.836), and day 30 (p = 0.371) showed no significant difference (p-value > 0.05). Comparison of the mean difference of the TAOC levels in two groups between day 0 with day 15 was p = 0.946, day 0 with day 30 was p = 0.504, and day 15 with day 30 was p = 0.811; which also showed no significant difference. Conclusion: There is no effect of green tea gel (Camellia sinensis) application on the Total Antioxidant Capacity (TAOC) level in chronic periodontitis treatment in the form of scaling and root planing.Keywords: Green tea gel, chronic periodontitis, scaling and root planing, total antioxidant capacity (TAOC).

The effect of solvent extraction on antioxidant properties of apricot fruit

2011 ◽  
Vol 6 (2) ◽  
pp. 199-204 ◽  
Author(s):  
Annamaria Leccese ◽  
Raffaella Viti ◽  
Susanna Bartolini
Keyword(s):  
Solvent Extraction ◽  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Aqueous Ethanol ◽  
Antioxidant Properties ◽  
Trolox Equivalent Antioxidant Capacity ◽  
Total Phenols ◽  
Fruit Samples ◽  
Total Antioxidant ◽  
Ethanol Ethanol

AbstractTwo solvent extraction procedures were used to investigate the extraction efficiency in terms of total antioxidant capacity and total phenols in apricot fruit. Samples were either sequentially extracted with aqueous ethanol (ethanol/water 80% v/v) and tetrahydrofuran or directly extracted with tetrahydrofuran. Each extract was analyzed for total antioxidant capacity by the Trolox Equivalent Antioxidant Capacity (TEAC) assay and total phenols by the Folin-Ciocalteu assay. The results showed that using sequential solvent extraction, the majority (85%) of the total antioxidant capacity and total phenols was due to hydrophilic compounds. In tetrahydrofuran direct extractions, the total antioxidant capacity and total phenols were higher than values obtained with aqueous ethanol and the sum of results obtained from sequential extracts for either total antioxidant capacity or total phenols was similar to the tetrahydrofuran-extract antioxidant values. A linear correlation between total antioxidant capacity and total phenols was found and was independent of the solvent extraction method. In conclusion, the choice of solvent is related to the antioxidant potential of fruit and depends on the food hydrophilic/lipophilic composition.

scholarly journals Investigation of Astaxanthin Effect on Cisplatin Ototoxicity in Rats by Using Otoacoustic Emission, Total Antioxidant Capacity, and Histopathological Methods

2019 ◽  
pp. 014556131986682 ◽  
Author(s):  
M. Emrah Kınal ◽  
Arzu Tatlıpınar ◽  
Selami Uzun ◽  
Serhan Keskin ◽  
Emrah Tekdemir ◽  
...  
Keyword(s):  
Protective Effect ◽  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Otoacoustic Emission ◽  
The Other ◽  
Female Rats ◽  
Control Group ◽  
Hearing Level ◽  
Significant Difference ◽  
Total Antioxidant

Background: Cisplatin-induced ototoxicity is related to oxidative stress. Astaxanthin is one of the most powerful antioxidants in nature. Aims/objectives: To investigate the protective effect of astaxanthin on cisplatin-induced ototoxicity. Materials and Methods: Thirty-five Sprague Dawley female rats were divided into 5 groups: control, cisplatin, and cisplatin with 10, 20, and 40 mg/kg astaxanthin groups. Cisplatin group received a single intraperitoneal injection of 14 mg/kg cisplatin. While saline was administered in the control group, in the other 3 groups, 10, 20, and 40 mg/kg daily doses of astaxanthin were administered through orogastric cannula before administration of cisplatin. Baseline and 10th day otoacoustic emission tests were administered. An intracardiac blood sample was taken to measure total antioxidant capacity (TAC), and the cochleas of the animals were investigated histopathologically. Results: Hearing level of astaxanthin 40 mg/kg + cisplatin group was higher at 24 kHz and 32 kHz frequencies compared to the cisplatin group. The TAC value of the cisplatin group was lower than both the control and astaxanthin + cisplatin groups ( P < .05). On histopathological examination, the other groups were deformed compared to the control group, but no statistically significant difference was observed between the astaxanthin + cisplatin and cisplatin groups. Conclusions and significance: Astaxanthin showed protective effect at high frequencies when it was administered at high dose. Thus, astaxanthin may have protective effect against cisplatin-induced ototoxicity.

scholarly journals Changes of Antioxidant Capacity of Robusta Coffee during Roasting

2009 ◽  
Vol 27 (Special Issue 1) ◽  
pp. S49-S52 ◽  
Author(s):  
L. Votavová ◽  
M. Voldřich ◽  
R. Ševčík ◽  
H. Čížková ◽  
J. Mlejnecká ◽  
...  
Keyword(s):  
Antioxidant Capacity ◽  
Chlorogenic Acid ◽  
Total Antioxidant Capacity ◽  
Radical Scavenging ◽  
Processing Plant ◽  
Total Phenols ◽  
Roasted Coffee ◽  
Robusta Coffee ◽  
Total Antioxidant

The <I>in vitro</I> radical scavenging capacity of the roasted and ground coffee is generally known as well as the published results of lowering the incidence of various diseases by regular intake of coffee. The antioxidant capacity of coffee is based mainly on the phenols, but during the roasting phenols are degraded and new products with antioxidant capacity are formed. A major contributor to the antioxidant activity was identified as N-methylpyridinium, which is formed during the roasting by degradation of trigonelline, the degradation is about 50% of trigonelline content and the concentration of N-methylpyridinium in roasted coffee is up to 0.25% on a dry weight basis. These literature data were verified within the processing plant experiment, during the usual roasting procedure of Robusta coffee the following parameters were analysed: humidity, water activity, total antioxidant capacity, total phenols, chlorogenic acid and trigonelline content, and colour <I>(L*, a*, b*</I>). The changes of the evaluated parameters were correlated to each other. During the roasting the total antioxidant capacity (TAC) decreased to about one half of original level in the beginning stages of roasting, another decrease continued during the storage of roasted coffee at about 10% within the year. The degradation of trigonelline, neither the content of chlorogenic acid or total phenols did not correlate with TAC in samples during the roasting and storage.

scholarly journals Plasma Total Antioxidant Capacity and Activities of Blood Glutathione Peroxidase and Superoxide Dismutase Determined in Healthy Dogs by Using Commercially Available Kits

2016 ◽  
Vol 66 (4) ◽  
pp. 534-548 ◽  
Author(s):  
Katerina Tomsič ◽  
Alenka Seliškar ◽  
Barbara Lukanc ◽  
Alenka Nemec Svete
Keyword(s):  
Superoxide Dismutase ◽  
Glutathione Peroxidase ◽  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Significant Difference ◽  
Erythrocyte Superoxide Dismutase ◽  
Total Antioxidant ◽  
Antioxidant Parameters ◽  
Healthy Dogs ◽  
Effect Of Age

AbstractData on the values of selected blood antioxidant parameters, i.e. total antioxidant capacity, glutathione peroxidase, and superoxide dismutase in healthy dogs, are lacking. There are no published accepted standard reference methods for their determination. The aim of this study was to determine the values of plasma total antioxidant capacity and the activities of whole blood glutathione peroxidase and erythrocyte superoxide dismutase in 30 healthy client-owned dogs (19 females, 11 males). The effect of age and sex on the measured antioxidant parameters was also investigated. Antioxidant parameters were determined with an automated biochemical analyser, using the commercially available Randox kits. No significant difference in age, weight, and antioxidant parameters was determined between females and males. A significant positive effect of age (p = 0.002, r2= 0.284) on superoxide dismutase activity was confirmed. There was no effect of sex on any of the antioxidant parameters measured. However, we observed a tendency of the effect of sex (p = 0.063, r2= 0.118), as well as age (p = 0.073, r2= 0.111), on the activity of glutathione peroxidase. Our results are in part comparable with the results of other studies in which the same types of methods and samples were used to determine antioxidant parameters. In conclusion, the sex and age of dogs should be taken into consideration when planning a study on antioxidant status parameters.

scholarly journals Evaluation of Total Antioxidant Capacity of Saliva in High School Students

2015 ◽  
Vol 8 (4) ◽  
pp. 89 ◽  
Author(s):  
Mahmoud Rahmani ◽  
Vahideh Ghorchi ◽  
Fatemeh Rezaei ◽  
Asad Vaisi-Raygani
Keyword(s):  
High School ◽  
High School Students ◽  
Dental Caries ◽  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Control Group ◽  
School Students ◽  
Significance Level ◽  
Significant Difference ◽  
Total Antioxidant

<p><strong>BACKGROUND &amp; OBJECTIVE: </strong>Imbalance between oxidative stress and saliva antioxidants plays a major role in initiation and spread of dental caries. The aim of this study was to evaluate the total antioxidant capacity (TAC) of saliva in dental caries.</p><p><strong>METHODS: </strong>In this case-control study which employed high school students (14-18 years), the un-stimulated saliva samples of 60 students without dental caries (control group) and 60 students with dental caries (with at least 5 teeth levels of dental caries) were gathered. Each group comprised of males (half of cases) and females (half of cases). TAC of saliva was measured by Zellbio® (Netherlands) in terms of micmol/L. The data were analyzed using the SPSS software (ver. 17.0) and t-test with considering significance level at 0.05.</p><p><strong>RESULTS</strong><strong>:</strong> TAC of saliva was significantly lower in cases with dental caries (0.256±0.106) compared to those without dental caries (0.396±0.077); P&lt; 0.001. There was no statistically significant difference of TAC of saliva between males (0.319±0.119) and females (0.333±0.113) irrespective of dental caries (P=0.507).</p><p><strong>CONCLUSION: </strong>The results of the study indicated that there was a reverse association between dental caries and TAC of saliva.</p>

scholarly journals Intermittent Fasting Decreases Oxidative Stress Parameters and Increases Total Antioxidant Capacity

2021 ◽  
Vol 12 (5) ◽  
pp. 6763-6775
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Intermittent Fasting ◽  
Sprague Dawley ◽  
Oxidative Stress Parameters ◽  
Sprague Dawley Rats ◽  
Significant Difference ◽  
Total Antioxidant ◽  
Stress Parameters

Modern lifestyle with an unhealthy routine, including consumption of excessive food and less physical activity, is full of many complications like chronic and degenerative diseases. For driving a healthy life, following a prophylactic style is necessary. The simplest and available way is intermittent fasting (IF). IF can help weight management. IF may lower oxidative stress. Oxidative stress can be determined from the malondialdehyde (MDA) levels and tri acyl glycerol (TAG) in the blood. Also, it increases Total antioxidant capacity (TAC). This study aimed to determine the effect of both protocols, Alternate day fasting (ADF) and time-restricted feeding (TRF), on plasma MDA level, TAG concentration, and TAC level of Sprague Dawley rats. They were randomly assigned to 3 groups, i.e., control group (C), fasting group (ADF), fasting group (TRF). Fasting given in this research was ADF, comprising one day of fasting for 24 hours, alternating with one day of normal feed ad libitum in the span of 12 weeks, including fasting 12 hours for two days a week plus three days in the middle of the month. After 3-months, blood was taken for examination of MDA levels, TAG, and TAC levels. A significant difference among the three groups in MDA levels, TAG and TAC. (p<0.05 for all parameters). Groups conducted on intermittent fasting had lower levels of MDA, TAG concentration, and a high level of TAC. ADF was lower in MDA level and TAG concentration than TRF. ADF was higher in TAC level than TRF. Intermittent fasting (ADF and TRF) decreases the oxidative stress parameters and increases Total antioxidant capacity in Sprague Dawley rats. ADF is more effective than TRF.

scholarly journals Large Scale Measurement of the Total Antioxidant Capacity of Different hungaricum Fruit and Vegetable Samples with Photochem Chemiluminometric Device

2005 ◽  
pp. 300-304
Author(s):  
Zsuzsanna Veres ◽  
Éva Domokos-Szabolcsy ◽  
Gábor Miklós Fári
Keyword(s):  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Large Scale ◽  
Sour Cherry ◽  
Water Soluble ◽  
Fruit And Vegetable ◽  
Green Pepper ◽  
Model Studies ◽  
Total Antioxidant ◽  
Breeding Programmes

In our present study, we performed a quick test of the antioxidant capacity of domestic fruit and vegetable cultivars with a PHOTOCHEM® instrument (Analytik Jena AG, Germany). The instrument is special because it can measure both the total lipid-soluble antioxidant capacity (ACL method) and the total water-soluble antioxidant capacity. Another advantage of the system is its quick measurement. With similar instruments, such measurement can take several hours, while PHOTOCHEM® can perform it within a few minutes.In our model studies, we examined the changes in the antioxidant capacity of sour cherry, green pepper, carrot, pumpkin, red beet and Brassica sp. cultivars. We aimed to determine how suitable the instrument is for quick, routine measurements in the case of the different horticultural products and which species have high antioxidant capacity values.On the basis of our examinations, we can state that the PHOTOCHEM® system is suitable for routine, semi-automated measurements of the total antioxidant capacity at several vegetables and fruits. Besides this, the system can be used for testing the quality of food products, for monitoring storability in post-harvest technologies, and for special breeding programmes and for physiological and biotechnological studies.

scholarly journals Assessment of Antioxidant Status and Lipid Peroxidation in Pre-Eclampsia

2019 ◽  
pp. 1-9
Author(s):  
Patrick O. Manafa ◽  
Charles C. Onyenekwe ◽  
Anselem C. Igwe ◽  
Nancy A. Mbachu ◽  
George O. Chukwuma ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Superoxide Dismutase ◽  
Glutathione Peroxidase ◽  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Positive Correlation ◽  
Spectrophotometric Methods ◽  
Significant Difference ◽  
Total Antioxidant ◽  
Blood Pressures

Aim: To assess the role of antioxidants and lipid peroxidation in the pathogenesis of pre-eclampsia. Study Design: This is a case-control study designed to evaluate the levels of malondialdehyde and total antioxidant capacity with glutathione peroxidase and superoxide dismutase activities in pre-eclamptic. One hundred (100) participants were randomly selected to include fifty pre-eclamptic (test), twenty-five (25) healthy pregnant normotensive women (control A) and twenty-five healthy non-pregnant normotensive women (control B). Place and Duration of Study: This study was carried out at the Holy Rosary Hospital waterside, Onitsha, Anambra state. Nigeria. The study lasted for 13 months. Methodology: One hundred women (50 pre-eclamptic, 25 pregnant normotensives and 25 non-pregnant normotensives) aged 25-40 years were recruited for this study. Blood samples were collected from the participants for the estimation of total antioxidant capacity (TAC), malondialdehyde (MDA), superoxide dismutase (SOD) and glutathione peroxidase (GPx) activities using spectrophotometric methods. Blood pressures were measured using accosson sphygmomanometer. Data were analysed using analysis of variance and Pearson’s correlation coefficient at p < 0.05. Results: The TAC was significantly higher in the pregnant normotensives (p< 0.05) compared with the pre-eclamptic and non-pregnant normotensives. There was no significant difference in the MDA in pre-eclamptic compared with pregnant normotensives and non-pregnant normotensives (p>0.05). A significant difference was observed in the SOD and GPx activities in the pregnant normotensive and non-pregnant normotensives compared with pre-eclamptic. A positive correlation was observed between the blood pressures (systolic and diastolic) and the activities of TAC, SOD and GPx in pre-eclamptic. Furthermore, a positive correlation was observed between MDA levels and SBP (r = 0.019) while a negative correlation (r = -0.225) existed between MDA and DBP. Conclusion: Diminished ability of antioxidants to scavenge free radicals may affect the onset of pre-eclampsia and therefore possible prognostic tool in its management.

scholarly journals Comparison of antioxidant activity, content of polyphenols and flavonoids in liturgical and common wines

10.5219/1030 ◽  
2019 ◽  
Vol 13 (1) ◽  
pp. 218-223
Author(s):  
Jiří Mlček ◽  
Anna Adámková ◽  
Soňa Škrovánková ◽  
Martin Adámek ◽  
Monika Ontrášová
Keyword(s):  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Total Content ◽  
White Wine ◽  
Biologically Active Substances ◽  
Pinot Noir ◽  
Biologically Active ◽  
Significant Difference ◽  
Total Antioxidant ◽  
Active Substances

This article deals with the comparison of biologically active substances (antioxidant capacity, content of polyphenols and flavonoids) in samples of common and liturgical wines. For determination were chosen these varieties Pinot Noir, Red Traminer and Chardonnay. The total content of polyphenols and flavonoids were found by visible diode array spectrophotometer. For determination of total antioxidant capacity was used the DPPH test. Results of this paper did not prove a general difference between liturgical and common wines, although between individual samples a statistically significant difference was found. Furthermore, the results show considerably higher values of biologically active substances in red wines Pinot Noir against white wine Red Traminer and Chardonnay – the total antioxidant capacity was considerably in excess of values up to 30 times, the total content of polyphenols up to 50 times and the total content of flavonoids up to 50 times. From the content of biologically active substances point of view, the red wine is recommended for human health.

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