scholarly journals Biomedical potential of horehound extract (Marrubium vulgare, Lamiaceae)

2018 ◽  
Vol 71 (1-2) ◽  
pp. 21-26 ◽  
Author(s):  
Nebojsa Salaj ◽  
Jelena Barjaktarovic ◽  
Nebojsa Kladar ◽  
Neda Gavaric ◽  
Biljana Bozin
Keyword(s):  
Lipid Peroxidation ◽  
Phenolic Compounds ◽  
Antioxidant Properties ◽  
Anticholinesterase Activity ◽  
Total Phenolic ◽  
Alcoholic Extract ◽  
Dry Extract ◽  
Marrubium Vulgare ◽  
Reducing Potential

Introduction. Horehound (Marrubium vulgare, Lamiaceae) is a widely used plant in traditional medicine used for prevention and treatment of various diseases. High content of phenolic compounds makes it a significant source of natural antioxidants. The aim of this research was to examine in vitro antioxidant properties and anticholinesterase activity of horehound water-alcoholic extract, followed by preliminary chemical characterization of horehound. Material and Methods. The in vitro antioxidant potentials of horehound water-alcoholic extract were assessed using several antioxidant testsystems (neutralization of 2,2-diphenyl-1-picrylhydrazyl, hydroxyl, and nitroso radical, determination of ferric reducing potential, as well as inhibition of lipid peroxidation). Preliminary chemical profiling of the extract included estimation of total phenolic and flavonoid contents, while anticholinesterase potential of the examined extract was evaluated by spectrophotometry. Results. The amounts of total phenolics and total flavonoids in the obtained extract were 59.87 ? 7.31 mg gallic acid equivalents/g of dry extract and 14.47 ? 0.54 mg quercetin equivalents/g of dry extract respectively. Furthermore, significant antioxidant potential was noticed in the ferric reducing potential assay (64.07 ? 2.68 mg ascorbic acid equivalents/g of dry extract), while concentrations needed for neutralization of 50% (IC50) of generated 2,2-diphenyl-1-picrylhydrazyl, nitroso and hydroxyl were 13.41 ?g/mL, 64.86 ?g/mL and 63.99 ?g/mL, respectively. The potential of the extract to inhibit lipid peroxidation process was modest (IC50 = 823.82 ?g/mL), while in the case of anticholinesterase potential, the recorded IC50 value was 2821.15 ?g/mL. Conclusion. Horehound represents a significant natural antioxidant, mostly as a result of high levels of phenolic compounds. In addition, the examined ethanol extract has a certain anticholinesterase activity.

scholarly journals Phytochemical Characterization, In Vitro Antioxidant Activity, and Quantitative Analysis by Micellar Electrokinetic Chromatography of Hawthorn (Crataegus pubescens) Fruit

2018 ◽  
Vol 2018 ◽  
pp. 1-11 ◽  
Author(s):  
Francisco Erik González-Jiménez ◽  
Juan Alfredo Salazar-Montoya ◽  
Graciano Calva-Calva ◽  
Emma Gloria Ramos-Ramírez
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Micellar Electrokinetic Chromatography ◽  
Antioxidant Properties ◽  
Total Phenolic ◽  
Electrokinetic Chromatography ◽  
Polyphenol Profile ◽  
Gallic Acid Equivalent ◽  
Dry Fruit

Due to their antioxidant properties, polyphenolic compounds are considered beneficial for human health. In this work, we investigated the polyphenol profile and antioxidant activity of edible tejocote (Crataegus pubescens) fruit extracts by micellar electrokinetic chromatography (MEKC) and HPLC/UV. The major phenolic compounds in the pulp extracts were (+)-catechin (9.17 ± 0.20 mg/100 mg dry fruit), (−)-epicatechin (4.32 ± 0.11 mg/100 mg dry fruit), and chlorogenic acid (5.60 ± 0.24 mg/100 mg dry fruit). The total phenolic content was 168.6 ± 0.9 mg gallic acid equivalent/g dry fruit; the total proanthocyanidin content was 84.6 ± 1.4 mg cyanidin/100 g dry fruit; and the total flavonoid content was 55.89 ± 1.43 mg quercetin/g dry fruit. Interestingly, procyanidins (dimers, trimers, and tetramers of (−)-epicatechin and (+)-catechin) were detected in the extract. This is the first study reporting the presence of polymeric polyphenols in Crataegus pubescens fruit. Accordingly, these fruits demonstrate great potential as a natural source of antioxidant phenolic compounds and could therefore be used as a nutraceutical and functional food.

scholarly journals A comparative study of phenolic compound antioxidant activity by the polarography method, using microsomal lipid peroxidation in vitro

2018 ◽  
Vol 31 (4) ◽  
pp. 186-189
Author(s):  
Anatolii Gordiienko ◽  
Mykola Blazheyevskyi ◽  
Ivan Iurchenko
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Chemical Structure ◽  
Quantitative Estimation ◽  
Antioxidant Properties ◽  
Microsomal Lipid Peroxidation ◽  
Phenolcarboxylic Acids ◽  
Inhibition Ability

Abstract For comparative purposes, a quantitative estimation of antioxidant activity of phenolic compounds of different classes was conducted by way of the polarography method, via the ADP-Fe2+ model of the induced ascorbate-dependent lipid peroxidation of rat liver micro-somes within an in-vitro system. As a result, it was recognized that the antioxidant properties of phenolic compounds depend on the nature and chemical structure of several substances. In respect of such activity, leaders in the classes of investigated polyphenolic compounds are: Propyl gallate = Gallotannin (Phenolcarboxylic acids and their derivatives) > Quercetin = Myricetin (Flavonols) > Luteolin (Flavo n) = Mangiferin (Xanthones) > Kaempferol (Flavonols) = Catechin (Flavans). Thus, the assessment of the inhibition ability of the lipid peroxidation of microsomes by phenolic compounds can be used as an accessible test for the preliminary quantitative estimation of their antioxidant properties.

scholarly journals Determination of Quality Characteristics, Phenolic Compounds and Antioxidant Activity of Propolis from Southeastern Mexico

2021 ◽  
Vol 65 (1) ◽  
pp. 109-122
Author(s):  
Enrique Sauri-Duch ◽  
Cesia Gutiérrez-Canul ◽  
Luis F. Cuevas-Glory ◽  
Lorena Ramón-Canul ◽  
Emilio Pérez-Pacheco ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Geographical Location ◽  
Principal Component ◽  
Total Phenolic ◽  
Dry Extract ◽  
Extract Content ◽  
The Individual

Abstract The objective of this work was to investigate the variability of physicochemical parameters, phenolic compounds and in vitro antioxidant activity of propolis collected from different apiaries in southeastern Mexico. A high variability was found in the moisture content (1.96–8.26%), ash (0.66–5.50%) and sensory characteristics of raw propolis from southeastern Mexico, but the raw propolis samples met the requirements of the quality regulations. In the same way, most of the ethanolic extracts also complied with the quality regulations. Of all the extracts, PE2 obtained from Santa Cruz showed the highest values for dry extract, content of total phenolic compounds (TPC), total flavonoids (TF) and antioxidant activity (DPPH and ABTS). The content of the individual phenolic compounds varied according to the geographical location of the apiary, but the PE2 extract resulted in the highest pinocembrin and chrysin content. A positive correlation was obtained between TPC and TF with antioxidant activity. Propolis extracts were classified into two groups through principal component analysis (PCA). These results indicate that the apiary location in southeastern Mexico influenced the characteristics of propolis.

scholarly journals Antioxidant and Antimutagenic (Anticlastogenic) Activity of Alcoholic Extract of Bauhinia variegata (Linn.) Root

2020 ◽  
pp. 32-39
Author(s):  
Dharmesh K. Golwala ◽  
Santosh Kumar Vaidya ◽  
Kishor K. Dholwani ◽  
Darpini S. Patel ◽  
Satyajit Sahoo
Keyword(s):  
Phenolic Compounds ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Alcoholic Extract ◽  
Root Extraction ◽  
Phytochemical Investigation ◽  
Bauhinia Variegata

Aims: Antioxidant and Antimutagenic (Anticlastogenic) activity of alcoholic extract of Bauhinia variegata (Linn.) root. Place: C. U. Shah College of Pharmacy and Research, Wadhwan, Surendranagar, Gujarat, India. Methodology: Shade dried Bauhinia variegata (Linn.) root, extraction was carryout by isolation extract were subjected to primary and secondary Phytochemical investigation. Then In-vitro antioxidant properties were estimated by reducing power and nitric oxide free radical scavenging method. Based on Phytochemical constituent and antioxidant properties In-vivo Antimutagenic (Anticlastogenic) activity was performed. Results: Preliminary phytochemical investigation revealed the presence of carbohydrates, free amino acids, and secondary metabolites like tannins, phenolic compounds and flavonoids, then polyphenol estimation found ALBV contains 86.38% phenolic compounds. In antioxidant properties determination IC50 respectively found 55.27±2.57 µg/ml, 125.52±8.15 µg/ml against of Ascorbic acid and Curcumin. Then In-vivo Antimutagenic (Anticlastogenic) activity ALBV shows significant reeducation in % MNPCE, % MNNCE and P/N ratio at 24 h, 48 h and 72 h against the cyclophosphamide-induced mutagenicity. Conclusion: Therefore, from the present study, it is concluded that alcoholic extract of Bauhinia variegata root (ALBV) can prove to be a very good antioxidant and effective chemopreventive against cyclophosphamide-induced mutagenesis.

scholarly journals Phenolic Compounds of Soybean Seeds from Two European Countries and Their Antioxidant Properties

Molecules ◽  
2020 ◽  
Vol 25 (9) ◽  
pp. 2075
Author(s):  
Angelika Król-Grzymała ◽  
Ryszard Amarowicz
Keyword(s):  
Phenolic Compounds ◽  
Defense Mechanism ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Cold Climate ◽  
Total Phenolic ◽  
Soybean Seeds ◽  
Phenolic Contents ◽  
Soybean Cultivars

There is only a small acreage of planted soybeans in northern Europe, as the global production of this crop is mainly dictated by the warmer temperatures needed for bountiful yields. The defense response of soybean plants to a cold climate entails the secretion of specific compounds which help mitigate oxidative stress, i.e., antioxidants, including phenolic compounds. The objective of this study was to examine differences in the concentrations of phenolic compounds, their antioxidant properties, and the concentration of key isoflavones (namely genistein, daidzein, malonyl daidzein, malonyl genistein, and daidzin) in the seeds of six soybean cultivars from two different regions of Europe, namely Poland and France. The total phenolic contents, isoflavone levels, and in vitro antioxidant capacities of soybean seeds from most of the investigated cultivars of northeast Europe were found to be greater than those from southwest Europe. The phenolic compounds of seed extracts are primarily responsible for the free-radical scavenging of soybeans. Factors regulating the production of phenolic compounds in the seeds have not been thoroughly elucidated. Hence, the results presented in this paper can be useful in the selection of soybean cultivars with higher levels of seed phenolics, because of their beneficial impact on human health and on the soybean’s defense mechanism against plant stresses.

scholarly journals Antioxidant and Anti-Inflammatory Potential and Consumer Acceptance of Wafers Enriched with Freeze-Dried Raspberry Pomace

2021 ◽  
Vol 11 (15) ◽  
pp. 6807
Author(s):  
Urszula Szymanowska ◽  
Monika Karaś ◽  
Justyna Bochnak-Niedźwiecka
Keyword(s):  
Phenolic Compounds ◽  
Antioxidant Properties ◽  
In Vitro Digestion ◽  
Biologically Active ◽  
Total Phenolic ◽  
Waste Products ◽  
Bioavailable Fraction ◽  
Freeze Dried ◽  
Anti Inflammatory

In this study, the effect of the addition of freeze-dried raspberry pomace on the content of phenolic compounds and the antioxidant and anti-inflammatory activity of wafers was investigated. Particular attention was paid to the biological activity of the potentially bioavailable fraction of polyphenols extracted via gastro-intestinal digestion. In the basic recipe for the waffle dough, flour was replaced with freeze-dried raspberry pomace in the amount of 10%, 20%, 30%, 50%, and 75%. The content of total phenolic compounds, phenolic acids, flavonoids, and anthocyanins in ethanol and buffer extracts and after in vitro digestion increased with the increase in the addition of pomace. A similar relationship was noted for antioxidant properties: ability to neutralize ABTS—2,2′-Azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) and DPPH—1,1-diphenyl-2-picrylhydrazyl radicals, iron II chelating ability, and reduction power. The extracts obtained after the simulated digestion showed the highest activities, which confirms that the polyphenols are a potentially bioavailable fraction. Extracts from the fortified wafers effectively inhibited the activity of enzymes involved in the generation of free radicals and induction of inflammation, i.e., xanthine oxidase (XO), lipoxygenase (LOX), and cyclooxygenase 2 (COX-2). The lowest IC50 values were determined for extracts after in vitro digestion. The sensory evaluation of the prepared wafers showed that the wafers fortified with 20% pomace achieved optimal scores. Enrichment of confectionery products with waste products from the fruit and vegetable industry can be a good way to increase the proportion of biologically active polyphenols in the diet and brings benefits to the environment.

scholarly journals Physicochemical characterization and antioxidant activity of Calabura fruit (Muntingia calabura L.)

2016 ◽  
Vol 7 (2) ◽  
pp. 67 ◽  
Author(s):  
Gustavo Araujo Pereira ◽  
Pedro Henrique Ferreira Tomé ◽  
Henrique Silvano Arruda ◽  
Edson José Fragiorge ◽  
Paulo Roberto Ribeiro
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Vitamin C ◽  
Antioxidant Properties ◽  
Physicochemical Characterization ◽  
Titratable Acidity ◽  
Soluble Solids ◽  
High Yield ◽  
Total Phenolic

The objective of this work was to determine the physical and physicochemical characteristics, proximate composition, antioxidant activity, total phenolic compounds, vitamin C content and total anthocyanins of different fractions of the calabura fruits (peel, pulp and whole fruit). The fruits showed average diameter and weight of 14.50 mm and 1.57 g, respectively, and high yield in pulp (75.38%). The whole fruit showed: pH (5.64), titratable acidity (0.11 g citric acid 100 g-1), soluble solids (10.24 °Brix), ratio (93.76), moisture (77.36%), ash (5.65%), lipids (7.79%), proteins (8.29%), crude fiber (5.93%), carbohydrates (72.15%), vitamin C (3.30 mg 100 g-1), phenolic compounds (526.55 mg tannic acid equivalents (TAE) 100 g-1), anthocyanins (4.08 mg cyanidin-3-glucoside equivalents (CGE) 100 g-1) and DPPH IC50 (82.25 μg mL-1).The peel showed high antioxidant activity (82.00 μg mL-1) due to the highest contents of phenolic compounds (868.90 mg TAE 100 g-1), anthocyanins (3.87 mg CGE 100 g-1) and vitamin C (4.20 mg 100 g-1). In conclusion, the calabura fruit showed high content of bioactive compounds with antioxidant properties in vitro.

scholarly journals Phenolic Compounds and Antioxidant Properties of Field-Grown and In Vitro Leaves, and Calluses in Blackberry and Blueberry

Horticulturae ◽  
2021 ◽  
Vol 7 (11) ◽  
pp. 420
Author(s):  
Tijana Kolarević ◽  
Danijel D. Milinčić ◽  
Tatjana Vujović ◽  
Uroš M. Gašić ◽  
Ljiljana Prokić ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Antioxidant Properties ◽  
Callus Cultures ◽  
Total Phenolic ◽  
Ms Medium ◽  
Scavenging Activity ◽  
Dpph Scavenging Activity ◽  
Dpph Scavenging ◽  
Blueberry Leaves

The aim of this study was to evaluate the content and profile of the phenolic compounds (PCs) and antioxidant properties of field-grown leaves, in vitro leaves and in vitro callus cultures of the blackberry ‘Čačanska Bestrna’ and blueberry ‘Toro’. In vitro shoots of the selected genotypes were grown either on original Murashige and Skoog (MS) medium containing 1 mg/L BA, 0.1 mg/l IBA and 0.1 mg/l GA3 (‘Čačanska Bestrna’) or on MS medium with macroelements reduced to ½, 2 mg/L zeatin and 0.2 mg/L IAA (‘Toro’). Callus cultures were induced from in vitro leaves and established on MS medium with 2 mg/L BA and 2 mg/L 2,4-D (‘Čačanska Bestrna’) or MS medium with half strength macroelements, 2 mg/L BA, 2 mg/L 2,4-D and 1 mg/L NAA (‘Toro’). Total phenolic (TPC) and flavonoid content (TFC) were the highest in blueberry leaves, whereas low TPC and TFC values were obtained in callus cultures of both cultivars. A higher content of PCs in blueberry leaves compared to blackberry leaves was determined by the UHPLC-DAD MS/MS technique. Quercetin derivatives and phenolic acids were the dominant PCs in the leaves of both berries, whereas gallocatechin was present in a significant amount in blueberry leaves. Callus cultures of both berries had a specific PC profile, with none detected in the leaves except quercetin-3-O-glucoside and quercetin-3-O-rutinoside. Blackberry leaves showed the best antioxidant properties as estimated by ferric reducing power (FRP), ABTS•+ and DPPH• scavenging activity assays. Callus cultures of both berries exhibited three to five times lower ABTS•+ and ten to seventeen times lower DPPH• scavenging activity compared to corresponding leaves. The analyzed leaves and callus cultures can be a good source of PCs with good antioxidant properties and specific phenolics, respectively, for applications in the food and pharmaceutical industries.

Sign in / Sign up

Export Citation Format

Share Document