scholarly journals Apoptosis as form of natural ovarian cell death

2004 ◽  
Vol 58 (1-2) ◽  
pp. 43-54
Author(s):  
Anita Radovanovic ◽  
Jelka Stevanovic ◽  
Dusan Gledic
Keyword(s):  
Ovarian Function ◽  
Morphological Changes ◽  
Dynamic Balance ◽  
Germinal Epithelium ◽  
Postnatal Life ◽  
Ovarian Cells ◽  
Reproductive Life ◽  
The Given ◽  
Epithelium Cells ◽  
Selection Of

Different hormones, cytokines, the absence of growth factors, and others, are some of the signals for initiating apoptosis in ovarian cells. Each of them in its own way, trigger apoptosis as a form of death in which the cell actively participates by precisely implementing a genetically programmed sequence of biochemical and morphological changes which lead to selfdestruction. Apoptosis is a physiological form of death, which helps establish a dynamic balance among proiliferation, differenciation, and death of ovarian cells. It has been confirmed so far that follicular cells oocytes, cells of the germinal epithelium, theca cells, and corpus luteum cells die through apoptosis. The physiological deaths of these cells are an integral part of normal ovarian function, both during intrauterine and postnatal life. Namely, during intrauterine ovarian development, about half the total number of germinative cells (future oocytes) die through apoptosis and their population is gradually reduced after birth by so-called selection of follicles which will continue further growth (folliculogenesis) and the apoptosis of cells of those follicles which will be subjected to atresion. Most ovarian cells die by apoptosis continuously until the end of the reproductive life period of healthy females, and some can continue dieing in this way until the death of the given individual (e.g. germinal epithelium cells).

Application of the turbidity ratio method in the spherical particle size determination in the range m ∈ and α ∈

1979 ◽  
Vol 44 (7) ◽  
pp. 2064-2078 ◽  
Author(s):  
Blahoslav Sedláček ◽  
Břetislav Verner ◽  
Miroslav Bárta ◽  
Karel Zimmermann
Keyword(s):  
Refractive Index ◽  
Spherical Particle ◽  
Ratio Method ◽  
Refractive Indices ◽  
Relative Refractive Index ◽  
Particle Size Determination ◽  
The Individual ◽  
The Given ◽  
Turbidity Ratio ◽  
Selection Of

Basic scattering functions were used in a novel calculation of the turbidity ratios for particles having the relative refractive index m = 1.001, 1.005 (0.005) 1.315 and the size α = 0.05 (0.05) 6.00 (0.10) 15.00 (0.50) 70.00 (1.00) 100, where α = πL/λ, L is the diameter of the spherical particle, λ = Λ/μ1 is the wavelength of light in a medium with the refractive index μ1 and Λ is the wavelength of light in vacuo. The data are tabulated for the wavelength λ = 546.1/μw = 409.357 nm, where μw is the refractive index of water. A procedure has been suggested how to extend the applicability of Tables to various refractive indices of the medium and to various turbidity ratios τa/τb obtained with the individual pairs of wavelengths λa and λb. The selection of these pairs is bound to the sequence condition λa = λ0χa and λb = λ0χb, in which b-a = δ = 1, 2, 3; a = -2, -1, 0, 1, 2, ..., b = a + δ = -1, 0, 1, 2, ...; λ0 = λa=0 = 326.675 nm; χ = 546.1 : 435.8 = 1.2531 is the quotient of the given sequence.

scholarly journals A rapid and robust method for the cryopreservation of human granulosa cells

2021 ◽  
Author(s):  
Sarah Beschta ◽  
Katja Eubler ◽  
Nancy Bohne ◽  
Ignasi Forne ◽  
Dieter Berg ◽  
...  
Keyword(s):  
Granulosa Cells ◽  
Large Scale ◽  
Ovarian Function ◽  
Oocyte Retrieval ◽  
Cell Contact ◽  
Cellular Model ◽  
Preovulatory Follicle ◽  
Human Granulosa Cells ◽  
Ovarian Cells

AbstractHuman primary granulosa cells (GCs) derived from women undergoing oocyte retrieval can be cultured and used as a cellular model for the study of human ovarian function. In vitro, they change rapidly, initially resembling cells of the preovulatory follicle and then cells of the corpus luteum. They are derived from individual patients, whose different medical history, lifestyle and age lead to heterogeneity. Thus, cells can rarely be ideally matched for cellular experiments or, if available, only in small quantities. We reasoned that cryopreservation of human GCs may be helpful to improve this situation. Previous studies indicated the feasibility of such an approach, but low survival of human GCs was reported, and effects on human GC functionality were only partially evaluated. We tested a slow freezing protocol (employing FCS and DMSO) for human GCs upon isolation from follicular fluid. We compared cryopreserved and subsequently thawed cells with fresh, non-cryopreserved cells from the same patients. About 80% of human GCs survived freezing/thawing. No differences were found in cell morphology, survival rate in culture, or transcript levels of mitochondrial (COX4, OPA1, TOMM20), steroidogenic (CYP11A1, CYP19A1) or cell–cell contact genes (GJA1) between the two groups in cells cultured for 1–5 days. A proteomic analysis revealed no statistically significant change in the abundance of a total of 5962 proteins. The two groups produced comparable basal levels of progesterone and responded similarly to hCG with elevation of progesterone. Taken together, our results show this to be a rapid and readily available method for the cryopreservation of human GCs. We anticipate that it will allow future large-scale experiments and may thereby improve cellular studies with human ovarian cells.

scholarly journals Assessment of reproductive potential in patients after myomectomy

2021 ◽  
Vol 8 (1) ◽  
pp. 35-39
Author(s):  
Yan Van ◽  
Yuliya A. Romadanova ◽  
Alla A. Bakhvalova ◽  
Ekaterina V. Fedina ◽  
Aleksandr A. Zinov’yev ◽  
...  
Keyword(s):  
Surgical Approach ◽  
Ovarian Function ◽  
Reproductive Potential ◽  
Morphological Structure ◽  
Clinical Situation ◽  
Functional Restoration ◽  
Hormonal Profile ◽  
Abdominal Myomectomy ◽  
Time Required ◽  
Selection Of

The aim of the study is to assess the restoration of anatomical and functional integrity of the uterus and identify changes in hormonal profile in women after laparoscopic and abdominal myomectomy. Material and methods. 58 patients aged 36,2 5,9 years old with uterine myoma underwent myomectomy: 31 patients underwent laparoscopic myomectomy and 27 patients underwent abdominal myomectomy. The selection of surgical approach didnt depend on the size, the number and localization of uterine myomas and the patients concomitant pathology. The hormonal profile of the patients including AMH level was assessed befor myomectomy and in the 6 months after the operation. Postoperative assessment was performed on day 5th8th after surgery and in 1 and 6 months after myomectomy. Results. No significant differences were found in the processes of reparation of the uterus and in hormonal profile of the patients after laparoscopic or abdominal myomectomy. The time required for the patients reabilitation and for the restoration of the ovarian function and uterine morphological structure was similar in both groups. Conclusion. The hormonal profile of the patients and the anatomical and functional restoration of myometrium after myomectomy doesnt depend on the surgical approach if myomectomy is performed by an experienced surgeon after the correct assessment of the clinical situation.

scholarly journals THE STYLISTIC FUNCTIONING OF SCRIPTURAL ALLUSIONS IN AN EARLY MODERN ENGLISH PLAY “JOHAN BAPTYSTES PREACHYNGE” BY J. BALE

2020 ◽  
Vol 30 (6) ◽  
pp. 1001-1007
Author(s):  
A.A. Khavronich
Keyword(s):  
Early Modern ◽  
Early Modern English ◽  
Semantic Expansion ◽  
Early English ◽  
The Given ◽  
Selection Of

The given article analyzes the peculiarities of the stylistic functioning of allusions to the Holy Scripture within one religious play belonging to the modern early English period, namely “Johan Baptystes Preachynge” produced by a dramatist J. Bale. The analysis is performed from the standpoint of linguopoetics. We consider stylistic features via the correlation of form and meaning, dissection of the conceptual component, juxtaposition with medieval plays representing adaptations of the same scriptural plot. Within the framework of this analysis we identify and assess elements performing the function of impact incorporated into the scriptural allusions and estimate their role in the selection of other lexical units, construction of extended metaphors, syntactic shaping of particular fragments of the play. We draw a conclusion that via the extension of scriptural metaphorical complexes the author brings about a meaningful focus shift to ensure a protestant reinterpretation of the included biblical theses. A substantial share of stylistically marked elements undergoes semantic expansion and develops adherent connotations since they relate to the pivotal elements of the allusions.

scholarly journals Selection of the variant solution of forest road alignment conceptual design using multicriteria optimisation

2009 ◽  
pp. 165-176 ◽  
Author(s):  
Bogdan Stefanovic ◽  
Ivan Bjelanovic
Keyword(s):  
Conceptual Design ◽  
Multicriteria Optimization ◽  
Linear Distribution ◽  
Forest Road ◽  
Multicriteria Optimisation ◽  
Technical Production ◽  
The Given ◽  
Selection Of ◽  
Design Construction ◽  
Building Parameters

One of the three studied variant solutions of forest road conceptual design was selected based on the linear distribution of criteria, as the method of multicriteria optimization. The selection was performed with 25 parameters classified as economic, technical, production and social criteria. The parameters of technical criteria were grouped into design, construction and building parameters. Based on calculated nominal values of parameters by the given criteria, their ranking, comparison, point rating and scoring, the selected most favorable solution was variant 1.

scholarly journals Vulnerability analysis and method of selection of communication protocols for information transfer in Internet of Things systems

2021 ◽  
pp. 133-149
Author(s):  
Maryna Kolisnyk
Keyword(s):  
Internet Of Things ◽  
Initial Data ◽  
Information Transfer ◽  
System Analysis ◽  
Communication Protocols ◽  
Critical Systems ◽  
Smart Objects ◽  
Corporate System ◽  
The Given ◽  
Selection Of

The subject of study in the paper is the analysis of technologies, architectures, vulnerabilities and cyberattacks, communication patterns of smart objects, messaging models, and Internet of Things (IoT) / Web of Things (WoT) protocols for solving applied problems of critical and non-critical systems. The goal is to develop a method for selecting messaging models and application-level protocols in non-critical and critical multi-level IoT/WoT systems, provided that the type of access to intelligent objects is initially determined by the initial data, as well as analysis of vulnerabilities and attacks using these protocols. Objectives: to formalize the procedure for choosing communication protocols for IoT/WoT systems; analyze possible vulnerabilities of communication protocols; develop a method for selecting communication protocols for given initial data, depending on the selected type of communication template for smart objects; check practically the proposed method. The methods of research are methods of system analysis. The following results were obtained. The analysis of the features of communication protocols is conducted by comparing the main interrelated characteristics of IoT/WoT, the results of which are presented in the form of a table. A method has been developed for selecting communication protocols, depending on the selected type of communication template. The analysis of possible vulnerabilities of communication protocols and possible attacks using these protocols is conducted. The author has tested the method using the example of a corporate system (Smart House) based on the WoT concept. Findings. The scientific novelty of the results obtained is as follows: the analysis conducted in the paper shows that currently there is no unified approach to the choice of a messaging model and application-level protocols for building IoT/WoT, depending on the selected type of communication template for smart objects. The method for selecting communication protocols for the given conditions (for each IoT system its interaction pattern will correspond, depending on which components interact with each other), improved by the authors of the paper, makes it possible to simplify the task of using separate protocols for given IoT systems, considering vulnerabilities of protocols.

scholarly journals The Evaluation of Agricultural Land Use Sustainability in the Post-Socialist Camp Countries: Methodological and Practical Aspects

2021 ◽  
Vol 13 (2) ◽  
pp. 59-78
Author(s):  
Olena Kotykova ◽  
Mykola Babych
Keyword(s):  
Land Use ◽  
Agricultural Land ◽  
Sustainable Use ◽  
Correct Selection ◽  
Agricultural Land Use ◽  
Current State ◽  
Definition Of ◽  
The Given ◽  
Selection Of ◽  
Integral Indicator

An effective implementation of the sustainable use of agricultural land program is impossible without reliable results of the current state of the problem. In this sense, the correct selection of indicators and methods for determining the level of stability is important. The authors proposes the definition of the agricultural land use sustainability integral indicator based on three methodological approaches: the construction of the indicators system, each of them reflects some aspects of the land use sustainability at the macro level (according to the specific issues); the construction of the integral indicator for comparing the countries’ land use sustainability. According to the given methods it has been proved that agricultural land use in the countries of the post-socialist camp has a positive dynamics, but the sustainability indicators for all indicators have not achieved yet. This study was supported in part by the Erasmus SUPPA program – Jean Monnet Associations Application No 611556-EPP-1-2019-1-UA-EPPJMO-SUPPA.

scholarly journals A Generalization of the Blackwell–Ryll-Nardzewski Measurable Selection Theorem

2021 ◽  
Vol 134 (1) ◽  
pp. 35-41
Author(s):  
K.A. Afonin ◽  
◽  
Keyword(s):  
Metric Spaces ◽  
Transition Probability ◽  
Measurable Space ◽  
Measurable Selection ◽  
Selection Theorem ◽  
Borel Set ◽  
Borel Probability ◽  
Borel Mapping ◽  
The Given ◽  
Selection Of

One of the main forms of the measurable selection theorem is connected with the existence of the graph of a measurable mapping in a given measurable set 𝑆 in the product of two measurable spaces 𝑋 and 𝑌 . Such a graph enables one to pick a point in the section 𝑆𝑥 for each 𝑥 in 𝑋 such that the obtained mapping will be measurable. The indicated selection is called a measurable selection of the multi-valued mapping associating to the point 𝑥 the section 𝑆𝑥 , which is a set in 𝑌 . The classical theorem of Blackwell and Ryll-Nardzewski states that a Borel set 𝑆 in the product of two complete separable metric spaces contains the graph of a Borel mapping (hence admits a Borel selection) provided that there is a transition probability on this product with positive measures for all sections of 𝑆 . The main result of this paper gives a generalization to the case where only one of the two spaces is complete separable and the other one is a general measurable space whose points parameterize a family of Borel probability measures on the first space such that the sections of the given set 𝑆 in the product have positive measures.

scholarly journals Use of factors of photoperiod duration and growth regulators to increase the yield of mixoploid plants in C0 generation in meadow clover (Trifolium pratense L.)

2021 ◽  
Vol 901 (1) ◽  
pp. 012024
Author(s):  
M Yu Novoselov ◽  
L V Drobysheva ◽  
O A Starshinova
Keyword(s):  
Apical Dominance ◽  
High Efficiency ◽  
Trifolium Pratense ◽  
Morphological Changes ◽  
Continuous Illumination ◽  
Experimental Plant ◽  
Trifolium Pratense L ◽  
Naphthylacetic Acid ◽  
Spraying Plants ◽  
Selection Of

Abstract It has been established that the studied mode of cultivation of meadow clover under controlled environmental conditions, with continuous illumination with an intensity of 18-20 thousand lux and a constant temperature of + 25 °C, causes significant morphological changes in meadow clover. Compared with the optimal (control), the proposed growing regime halves the development time of plants and by the same amount reduces the severity of morphological traits, while simultaneously causing apical dominance in 54% of plants. Spraying plants with phytohormone in the form of naphthylacetic acid in all studied concentrations reduced the growing season by 4-8 days and increased pollen fertility by 8-10%. The optimal concentration of naphthylacetic acid (NLA) was revealed to suppress the growth of secondary meristems in meadow clover under the conditions of an experimental plant growing regime. The most significant effect on the manifestation of apical dominance was shown by the use of naphthylacetic acid at a concentration of 0.001%. The combined use of the proposed growing regime and treatment with naphthylacetic acid at a concentration of 0.001% increased the yield of plants with apical dominance from 54.5% to 76%. The developed method has shown high efficiency for the creation of tetraploid forms of meadow clover. When growing clover C0 generation and selection of mixoploid plants, the yield of mixoploids increased 5.4 times, the survival rate of seedlings increased by 21%, the time to obtain one generation and the selection of mixoploids decreased by 58%.

scholarly journals THE STATIC OPTIMIZATION TASK OF OPTIMAL DESIGN OF NONLINEAR ELECTRONIC SCHEME

2019 ◽  
pp. 109-115
Author(s):  
Didmanidze Ibraim ◽  
Donadze Mikheil
Keyword(s):  
Optimal Design ◽  
Optimality Criteria ◽  
Pareto Optimal Solutions ◽  
Optimal Solutions ◽  
Design Task ◽  
Optimization Task ◽  
Minimum Capacity ◽  
Optimal Values ◽  
The Given ◽  
Selection Of

The article deals with such an important selection of the elements of electronic scheme of the given configuration, when the certain requirements of technical task are satisfied and at the same time the selected optimality criteria reach the extreme value. The gives task has been solved by the method of one-criterion optimization, in particular, the method of center gravity. To formalize the given scheme we have compiled a mathematical model of optimization, which considers the requirements of technical task. The optimal design task of the presented electronic scheme was brought to the task of multi criteria optimization. The computational experiments have been resulted in the Pareto-optimal solutions, from which there was selected a compromise on that corresponds to the minimum capacity, required by the scheme. According to the optimal values of resistors, we have conducted a computerized analysis of the transient process of the given electronic scheme with the help of a computer program Electronics Workbench.

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