Effectiveness of platelet rich fibrin versus demineralized bone xenograft in periodontally accelerated osteogenic orthodontics: A pilot comparative clinical study

ABSTRACT Objectives To compare the rate of extraction space closure between periodontally accelerated osteogenic orthodontics (PAOO) using platelet-rich fibrin (PRF) (Group 1) and PAOO using demineralized bone xenograft (DMBM) (Group 2) and to compare the level of wound healing between the PRF group vs the DMBM group after PAOO. Materials and Methods A two-arm prospective single blind pilot study with a split-mouth design was used in which 14 patients requiring premolar extraction were divided into two groups: PRF and DMBM. En-masse space closure was carried out with using mini implants after the PAOO procedure. The amount of space closure was measured at five time points with 2-week intervals within 2 months. The gingival healing levels were assessed using early wound healing scores on the first postoperative day. Results The rate of extraction space closure was faster in the experimental quadrant at all time points (T1-T4) in the PRF group and at time points (T3, T4) in the DMBM group. Comparison between experimental quadrants showed a significant increase in the rate of space closure in the PRF group T1 to T3 (P < .05). The PRF group showed higher total early healing scores than the DMBM group. Conclusions PRF, when used in the PAOO procedure, produces a faster rate of space closure with better early wound healing than DMBM.

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Leucocyte‐ and platelet‐rich fibrin regulates expression of genes related to early wound healing in human gingival fibroblasts

Journal Of Clinical Periodontology ◽

10.1111/jcpe.13293 ◽

2020 ◽

Vol 47 (7) ◽

pp. 851-862

Author(s):

Jiarui Bi ◽

Maria Fernanda Barona Intriago ◽

Leeni Koivisto ◽

Guoqiao Jiang ◽

Lari Häkkinen ◽

...

Keyword(s):

Wound Healing ◽

Human Gingival Fibroblasts ◽

Gingival Fibroblasts ◽

Platelet Rich Fibrin ◽

Expression Of Genes ◽

Early Wound

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A Comparative Clinical Study on Surgical Blade and Diode Laser in the Treatment of Gingival Melanin Pigmentation

Journal of Evolution of Medical and Dental Sciences ◽

10.14260/jemds/2021/148 ◽

2021 ◽

Vol 10 (10) ◽

pp. 689-693

Author(s):

Somit Kumar Jain ◽

Nina Shenoy ◽

Mehul Kumar Chourasia ◽

Amitha Ramesh

Keyword(s):

Wound Healing ◽

Clinical Study ◽

Diode Laser ◽

Pain Perception ◽

Rank Test ◽

Side Group ◽

Melanin Pigmentation ◽

Comparative Clinical Study ◽

Group 2 ◽

Group 1

BACKGROUND Gingival melanin pigmentation (GMP) is a common aesthetic concern. The gold standard for depigmentation, is surgery. Recently soft tissue lasers have gained popularity. The purpose of this study was to compare and evaluate the clinical efficacy of surgical blade and laser treatment in terms of pain perception and wound healing outcomes, 7 and 14 days following the procedures for the treatment of gingival depigmentation. METHODS This 14 day follow up comparative clinical study, utilized a split mouth design, in the anterior region. Fifteen patients with moderate to heavy bilateral gingival pigmentation were included as per the criteria by Dummett et al. The patients’ right side (group 1) was treated with surgical blade and the left side (group 2) with diode laser (Picasso, AMD LASERS™) by a trained clinician. Pain perception was assessed using Visual Analog Scale (VAS) and wound healing was assessed using clinical parameters. RESULTS Data was analysed using paired t test and Wilcoxon’s signed rank test. P value of < 0.05 was considered statistically significant. On day 7, more than 70 % patients had slight pain in group 1. Whereas, 47 % experienced no pain in group 2. The mean difference in VAS scores between the groups at day 7, was statistically significant. No statistically significant distinction in wound healing was observed 7 days following both procedures. No pain, and complete epithelialisation was noted in both groups on day 14. CONCLUSIONS Pain experienced by the patients was slightly more in the scalpel group compared to laser. There was no difference in wound healing between both groups. KEY WORDS GMP, Gingival Melanin Pigmentation, VAS Scale, Depigmentation, Wound Healing, Laser, Surgical Blade, Split Mouth Design

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SPACE CLOSURE IN BIALVEOLAR DENTAL PROTRUSION CASES - A COMPARATIVE COMBINATION METHOD

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2017.v10i7.17504 ◽

2017 ◽

Vol 10 (7) ◽

pp. 106

Author(s):

Chaitanya N ◽

Arshad Am ◽

Praveen Krk ◽

Prashant C ◽

Sandeep Bailwad ◽

...

Keyword(s):

Clinical Study ◽

Combination Method ◽

Mini Implants ◽

Space Closure ◽

Lateral Cephalograms ◽

Comparative Clinical Study ◽

Implant Anchorage ◽

En Masse Retraction ◽

Anchor Loss ◽

Rate Of Movement

Objective: To measure and compare the amount, rate and anchor loss after the en masse retraction of all anteriors with titanium mini-implant anchorage and conventional molar anchorage.Methods: This comparative clinical study sample comprised 12 patients (10 females, 2 males; mean age between 16 and 22 years). The implants were placed in the maxillary and mandibular arches. Preretraction and post retraction lateral cephalograms were taken for measuring the amount, rate and anchor loss after the retraction.Results: Mean en masse retraction amounts, the rate of movement per month, and horizontal and vertical anchor loss at the maxillary implant site were 4.79 mm, 0.58 mm, 0 mm, and 0 mm, respectively. In the mandible, on implant sides were 4.66 mm, 0.56 mm, 0 mm, and 0 mm. Mean en masse retraction amounts, the rate of movement per month, and horizontal and vertical anchor loss at the maxillary conventional molar anchor side were 4.08 mm, 0.49 mm, 2.91 mm, and 1.66 mm. In the mandible, on conventional anchor sides were 3.54 mm, 0.48 mm, 3.12 mm, and 1.95 mm.Conclusion: En masse retraction had a faster rate of space closure with mini-implants as anchor units than the conventional molar anchorage preparation.

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A Comparative Clinical Study of Wound Healing Properties of Aloe Vera Gel and Datura Metel Extract in Traumatic Wounds in a Tertiary Care Hospital

Indian Journal Of Applied Research ◽

10.15373/2249555x/aug2013/196 ◽

2011 ◽

Vol 3 (8) ◽

pp. 613-615

Author(s):

V. P. Karthik V. P. Karthik ◽

◽

S. Seethalakshmi S. Seethalakshmi ◽

Durairaj Durairaj

Keyword(s):

Wound Healing ◽

Clinical Study ◽

Tertiary Care Hospital ◽

Tertiary Care ◽

Aloe Vera ◽

Care Hospital ◽

Datura Metel ◽

Aloe Vera Gel ◽

Comparative Clinical Study

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N-Acetylcysteine Added to Local Anesthesia Reduces Scar Area and Width in Early Wound Healing—An Animal Model Study

International Journal of Molecular Sciences ◽

10.3390/ijms22147549 ◽

2021 ◽

Vol 22 (14) ◽

pp. 7549

Author(s):

Wiktor Paskal ◽

Adriana M. Paskal ◽

Piotr Pietruski ◽

Albert Stachura ◽

Kacper Pełka ◽

...

Keyword(s):

Wound Healing ◽

Early Stage ◽

Healing Process ◽

Wound Healing Process ◽

Sprague Dawley ◽

Time Points ◽

Model Study ◽

Sprague Dawley Rats ◽

Animal Model Study ◽

Anesthetic Solution

The aim of the study was to evaluate if a pre-incisional N-acetylcysteine (NAC) treatment altered the process of wound healing in a rat model. The dorsal skin of 24 Sprague-Dawley rats was incised in six locations. Before the incisions were made, skin was injected either with lidocaine and epinephrine (one side) or with these agents supplemented with 0.015%, 0.03%, or 0.045% NAC (contralaterally). Photographic documentation of the wound healing process was made at 11 time points. Rats were sacrificed 3, 7, 14, or 60 days after incision to excise scars for histological analysis. They included: Abramov scale scoring, histomorphometry analysis, and collagen fiber arrangement assessment. Skin pretreated with 0.03% NAC produced the shortest scars at all analyzed time points, though this result was statistically insignificant. At this NAC concentration the scars had smaller areas on the third day and were narrower on the day 4 compared with all the other groups (p < 0.05). On day 7, at the same concentration of NAC, the scars had a higher superficial concentration index (p = 0.03) and larger dermal proliferation area (p = 0.04). NAC addition to pre-incisional anesthetic solution decreased wound size and width at an early stage of scar formation at all concentrations; however, with optimal results at 0.03% concentration.

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Topical Application of Ozonated Oils for the Treatment of MRSA Skin Infection in an Animal Model of Infected Ulcer

Biology ◽

10.3390/biology10050372 ◽

2021 ◽

Vol 10 (5) ◽

pp. 372

Author(s):

Vanessa Silva ◽

Cecília Peirone ◽

Rosa Capita ◽

Carlos Alonso-Calleja ◽

José A Marques-Magallanes ◽

...

Keyword(s):

Wound Healing ◽

Animal Model ◽

Topical Application ◽

Early Stage ◽

Bacterial Load ◽

Ozone Treatment ◽

Diabetic Patients ◽

Colony Forming Units ◽

Group 2 ◽

Epidermal Regeneration

Diabetic foot ulcers are a common cause of morbidity in diabetic patients. One of the main pathogens found in these ulcers is methicillin-resistant Staphylococcus aureus (MRSA). MRSA often carries resistance to several classes of antibiotics and their infections are becoming harder to treat. Therefore, new alternatives are urgently needed. Thus, this study aimed to investigate the capacity of topical ozonated oil application on the treatment of early-stage skin infected with MRSA in an animal model. Ozonated oil was prepared from a mixture of oils subjected to a gas stream of O2/O3 mixture. Sixteen Wistar rats were inoculated by an intradermic injection of MRSA suspension, producing an abscess lesion. After 3 days, the skin epidermis was removed to open the wound. Group 1 received an application of oil mixture without ozone treatment and Group 2 received an application of ozonated oil. After the treatment period, skin was collected, colony-forming units (CFU) of bacteria were quantified and the histological analysis of the skin was carried out. Skin samples from the control 1 and 2 had a bacterial load was of 1.1 × 105 and 5.7 × 103 CFU/mL, respectively. Group 2 showed better wound healing from mild to moderate epidermal regeneration. Topical application of ozonated vegetable oil in MRSA-infected skin in rats showed a small reduction of the bacterial load and better wound healing.

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Cutaneous wound healing is impaired in hemophilia B

Blood ◽

10.1182/blood-2006-05-020495 ◽

2006 ◽

Vol 108 (9) ◽

pp. 3053-3060 ◽

Cited By ~ 74

Author(s):

Maureane Hoffman ◽

Anna Harger ◽

Angela Lenkowski ◽

Ulla Hedner ◽

Harold R. Roberts ◽

...

Keyword(s):

Wound Healing ◽

Time Course ◽

Ferric Iron ◽

Hemophilia B ◽

Cutaneous Wound Healing ◽

Delayed Wound Healing ◽

Cutaneous Wound ◽

Time Points ◽

Hemophilic Arthropathy ◽

Healing Wounds

Abstract We used a mouse model to test the hypothesis that the time course and histology of wound healing is altered in hemophilia B. Punch biopsies (3 mm) were placed in the skin of normal mice and mice with hemophilia. The size of the wounds was measured daily until the epidermal defect closed. All wounds closed in mice with hemophilia by 12 days, compared with 10 days in normal animals. Skin from the area of the wound was harvested at different time points and examined histologically. Hemophilic animals developed subcutaneous hematomas; normal animals did not. Macrophage infiltration was significantly delayed in hemophilia B. Unexpectedly, hemophilic mice developed twice as many blood vessels in the healing wounds as controls, and the increased vascularity persisted for at least 2 weeks. The deposition and persistence of ferric iron was also greater in hemophilic mice. We hypothesize that iron plays a role in promoting excess angiogenesis after wounding as it had been proposed to do in hemophilic arthropathy. We have demonstrated that impaired coagulation leads to delayed wound healing with abnormal histology. Our findings have significant implications for treatment of patients with hemophilia, and also highlight the importance of rapidly establishing hemostasis following trauma or surgery.

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Effectiveness of Silicon Platelet-Rich Fibrin and Autologous Bone on Bone Regeneration in Rabbit Calvarian Defects: A Radiological and Histological Study

Applied Sciences ◽

10.3390/app11094074 ◽

2021 ◽

Vol 11 (9) ◽

pp. 4074

Author(s):

Argimiro Hernández-Suarez ◽

María Rizo-Gorrita ◽

Dubraska Suárez-Vega ◽

Gladys Velazco ◽

Ivan Rodriguez Gelfenstein ◽

...

Keyword(s):

Bone Regeneration ◽

Oral Surgery ◽

Histological Study ◽

Study Groups ◽

Control Group ◽

Graft Material ◽

Platelet Rich Fibrin ◽

Computed Microtomography ◽

Autologous Bone ◽

Group 2

Repairing bone defects in oral surgery often requires the use of bone regeneration techniques. Silicon is an element that has been employed as regeneration material in several studies. In our study, silicon was combined with autologous bone and platelet-rich fibrin (PRF) membranes to analyse the behaviour of this element in bone regeneration. Four circumferential defects were created in the cranial vault of five New Zealand rabbits. The following elements were applied to the regeneration of the defects: (P): PRF; (S): silicon and (B): autologous bone, with the following distribution of study groups: Group 1 (PSB); Group 2 (PS); Group 3 (SB) and Group 4 (CONTROL): unregenerate group. The animals were sacrificed after 3 weeks. Computed microtomography studies (μ-CT) were carried out, as well as histomorphometric ones. The ANOVA statistical test was used with a Bonferroni post-hoc test to compare the results (p ≤ 0.05). Radiologically, groups PSB and SB were better as far as quantity and percentage of healthy bone observed, but not significantly compared to the control group. The PS group was significantly worse. The histological test revealed that the PSB group was the one to present the largest area, percentage and perimeter of mineralised bone. On evaluating the forming bone (osteoid), no difference was observed across the groups with the exception of the bone perimeter, where the SB group was significantly better. The bone height variable showed no significant differences. In conclusion we can state that the combination of PRF, autologous bone and silicon provides good results at 3 weeks whilst the PS group shows the worst results. This highlights the importance of autologous bone forming part of the graft material in order for the bone to mineralise.

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