Research on Printed Conductive Silver Layer based on Inkjet Photo Paper

2021 ◽  
Author(s):  
Yi Fang ◽  
Lixin Mo ◽  
Zhiqing Xin ◽  
Yinjie Chen ◽  
Xiu Li ◽  
...  
Keyword(s):  
High Performance ◽  
Laser Scanning ◽  
Low Cost ◽  
Flexible Substrate ◽  
Electronic Devices ◽  
Laser Scanning Microscopy ◽  
Silver Layer ◽  
Confocal Laser ◽  
Nano Silver ◽  
Silver Ink

Printed electronics is an emerging technology that applies traditional printing or coating processes to the manufacture of electronic devices and products. In order to find a low-cost, high-performance, environmentally-friendly flexible substrate suitable for electronic devices, the printability between four kinds of inkjet photo papers and nano-silver ink was investigated. First, different surface morphologies of the inkjet photo papers were measured by a confocal laser scanning microscopy. Then, a pen and a gravure printer were used to test the printability between photo papers and nano-silver ink. It was found that the conductive track and pattern was influenced by the surface morphology of the photo papers. Furthermore, a four-probe test showed that the conductivity of the ink layers on the four photo papers was almost at the same level. Furthermore, a tearing test with 3 M tapes showed that the silk photo paper had the best tearing resistance. In general, silk photo paper has the best overall performance. This research could be beneficial for the development of flexible electronic devices which are low-cost, mass manufacture suitable and environment friendly.

scholarly journals Software-Based Three-Dimensional Deconvolution Microscopy of Cytoskeletal Proteins in Cultured Fibroblast Using Open-Source Software and Open Hardware

2019 ◽  
Vol 5 (12) ◽  
pp. 88
Author(s):  
Kazuo Katoh
Keyword(s):  
Open Source ◽  
Open Source Software ◽  
Laser Scanning ◽  
Low Cost ◽  
Three Dimensional ◽  
Cytoskeletal Proteins ◽  
Laser Scanning Microscopy ◽  
Confocal Laser ◽  
Actual Measurement ◽  
Open Hardware

As conventional fluorescence microscopy and confocal laser scanning microscopy generally produce images with blurring at the upper and lower planes along the z-axis due to non-focal plane image information, the observation of biological images requires “deconvolution.” Therefore, a microscope system’s individual blur function (point spread function) is determined theoretically or by actual measurement of microbeads and processed mathematically to reduce noise and eliminate blurring as much as possible. Here the author describes the use of open-source software and open hardware design to build a deconvolution microscope at low cost, using readily available software and hardware. The advantage of this method is its cost-effectiveness and ability to construct a microscope system using commercially available optical components and open-source software. Although this system does not utilize expensive equipment, such as confocal and total internal reflection fluorescence microscopes, decent images can be obtained even without previous experience in electronics and optics.

scholarly journals Polyamines Are Essential for the Formation of Plague Biofilm

2006 ◽  
Vol 188 (7) ◽  
pp. 2355-2363 ◽  
Author(s):  
Chandra N. Patel ◽  
Brian W. Wortham ◽  
J. Louise Lines ◽  
Jacqueline D. Fetherston ◽  
Robert D. Perry ◽  
...  
Keyword(s):  
High Performance ◽  
Laser Scanning ◽  
Double Mutant ◽  
Arginine Decarboxylase ◽  
Laser Scanning Microscopy ◽  
Confocal Laser ◽  
Crystal Violet Staining ◽  
Liquid Chromatography Mass Spectrometry ◽  
Double Deletion ◽  
Single Deletion

ABSTRACT We provide the first evidence for a link between polyamines and biofilm levels in Yersinia pestis, the causative agent of plague. Polyamine-deficient mutants of Y. pestis were generated with a single deletion in speA or speC and a double deletion mutant. The genes speA and speC code for the biosynthetic enzymes arginine decarboxylase and ornithine decarboxylase, respectively. The level of the polyamine putrescine compared to the parental speA + speC + strain (KIM6+) was depleted progressively, with the highest levels found in the Y. pestis ΔspeC mutant (55% reduction), followed by the ΔspeA mutant (95% reduction) and the ΔspeA ΔspeC mutant (>99% reduction). Spermidine, on the other hand, remained constant in the single mutants but was undetected in the double mutant. The growth rates of mutants with single deletions were not altered, while the ΔspeA ΔspeC mutant grew at 65% of the exponential growth rate of the speA + speC + strain. Biofilm levels were assayed by three independent measures: Congo red binding, crystal violet staining, and confocal laser scanning microscopy. The level of biofilm correlated to the level of putrescine as measured by high-performance liquid chromatography-mass spectrometry and as observed in a chemical complementation curve. Complementation of the ΔspeA ΔspeC mutant with speA showed nearly full recovery of biofilm to levels observed in the speA + speC + strain. Chemical complementation of the double mutant and recovery of the biofilm defect were only observed with the polyamine putrescine.

scholarly journals Progress towards Sustainable Control of Xylella fastidiosa subsp. pauca in Olive Groves of Salento (Apulia, Italy)

Pathogens ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 668
Author(s):  
Marco Scortichini ◽  
Stefania Loreti ◽  
Nicoletta Pucci ◽  
Valeria Scala ◽  
Giuseppe Tatulli ◽  
...  
Keyword(s):  
Control Strategy ◽  
Laser Scanning ◽  
Vascular System ◽  
Xylella Fastidiosa ◽  
Low Cost ◽  
Interdisciplinary Studies ◽  
Laser Scanning Microscopy ◽  
Confocal Laser ◽  
Olive Groves

Xylella fastidiosa subsp. pauca is the causal agent of “olive quick decline syndrome” in Salento (Apulia, Italy). On April 2015, we started interdisciplinary studies to provide a sustainable control strategy for this pathogen that threatens the multi-millennial olive agroecosystem of Salento. Confocal laser scanning microscopy and fluorescence quantification showed that a zinc-copper-citric acid biocomplex—Dentamet®—reached the olive xylem tissue either after the spraying of the canopy or injection into the trunk, demonstrating its effective systemicity. The biocomplex showed in vitro bactericidal activity towards all X. fastidiosa subspecies. A mid-term evaluation of the control strategy performed in some olive groves of Salento indicated that this biocomplex significantly reduced both the symptoms and X. f. subsp. pauca cell concentration within the leaves of the local cultivars Ogliarola salentina and Cellina di Nardò. The treated trees started again to yield. A 1H-NMR metabolomic approach revealed, upon the treatments, a consistent increase in malic acid and γ-aminobutyrate for Ogliarola salentina and Cellina di Nardò trees, respectively. A novel endotherapy technique allowed injection of Dentamet® at low pressure directly into the vascular system of the tree and is currently under study for the promotion of resprouting in severely attacked trees. There are currently more than 700 ha of olive groves in Salento where this strategy is being applied to control X. f. subsp. pauca. These results collectively demonstrate an efficient, simple, low-cost, and environmentally sustainable strategy to control this pathogen in Salento.

scholarly journals A Selective Ratiometric Fluorescent Probe for No-Wash Detection of PVC Microplastic

Polymers ◽  
2021 ◽  
Vol 13 (10) ◽  
pp. 1588
Author(s):  
Valeria Caponetti ◽  
Alexandra Mavridi-Printezi ◽  
Matteo Cingolani ◽  
Enrico Rampazzo ◽  
Damiano Genovese ◽  
...  
Keyword(s):  
Laser Scanning ◽  
Direct Detection ◽  
Low Cost ◽  
Laser Scanning Microscopy ◽  
Confocal Laser ◽  
Wide Field ◽  
Fluorescence Intensity Ratio ◽  
Green Fluorescence ◽  
One Pot ◽  
Emerging Pollutant

Microplastics (MP) are micrometric plastic particles present in drinking water, food and the environment that constitute an emerging pollutant and pose a menace to human health. Novel methods for the fast detection of these new contaminants are needed. Fluorescence-based detection exploits the use of specific probes to label the MP particles. This method can be environmentally friendly, low-cost, easily scalable but also very sensitive and specific. Here, we present the synthesis and application of a new probe based on perylene-diimide (PDI), which can be prepared in a few minutes by a one-pot reaction using a conventional microwave oven and can be used for the direct detection of MP in water without any further treatment of the sample. The green fluorescence is strongly quenched in water at neutral pH because of the formation dimers. The ability of the probe to label MP was tested for polyvinyl chloride (PVC), polyethylene (PE), polyethylene terephthalate (PET), polypropylene (PP), polystyrene (PS), poly methyl methacrylate (PMMA) and polytetrafluoroethylene (PTFE). The probe showed considerable selectivity to PVC MP, which presented an intense red emission after staining. Interestingly, the fluorescence of the MP after labeling could be detected, under excitation with a blue diode, with a conventional CMOS color camera. Good selectivity was achieved analyzing the red to green fluorescence intensity ratio. UV–Vis absorption, steady-state and time-resolved fluorescence spectroscopy, fluorescence anisotropy, fluorescence wide-field and confocal laser scanning microscopy allowed elucidating the mechanism of the staining in detail.

scholarly journals Antimicrobial efficacy and biocompatibility of extracts from Cryptocarya species

PLoS ONE ◽  
2021 ◽  
Vol 16 (12) ◽  
pp. e0261884
Author(s):  
Jacqueline de Oliveira Zoccolotti ◽  
Alberto José Cavalheiro ◽  
Camilla Olga Tasso ◽  
Beatriz Ribeiro Ribas ◽  
Túlio Morandin Ferrisse ◽  
...  
Keyword(s):  
High Performance ◽  
Laser Scanning ◽  
Acrylic Resin ◽  
Laser Scanning Microscopy ◽  
Live Cells ◽  
Confocal Laser ◽  
Array Detector ◽  
Control Group ◽  
Oral Keratinocytes ◽  
Denture Base

This study evaluated the efficacy of Cryptocarya spp extracts on biofilm of Candida albicans and its biocompatibility. Mature biofilm of C. albicans was formed on denture base acrylic resin samples and the fungicidal effect of the extracts was evaluated by Alamar Blue® assay, counting colony-forming units (CFU/mL) and confocal laser scanning microscopy (CLSM). Cytotoxicity of extracts from Cryptocarya species was evaluated by AlamarBlue® assay, using normal oral keratinocytes (NOK) cells. In additional, Analysis of plant extracts by ultra-high-performance liquid chromatography–diode array detector–tandem mass spectrometry (UPLC-DAD-MS) was performed. The results showed significant reduction in the cellular metabolism and in the number of CFU/mL of C. albicans (p<0.05). The concentration of 0.045 g/mL completely inhibited the number of CFU/mL. Regarding cytotoxicity, all extracts decreased cell viability compared to the control group. CLSM analysis showed predominance of live cells, but with a great difference between the groups. Antimicrobial activity of extracts from Cryptocarya on C. albicans biofilm was confirmed. However, all extracts showed toxicity on NOK cells.

scholarly journals The Moss Leptodictyum riparium Counteracts Severe Cadmium Stress by Activation of Glutathione Transferase and Phytochelatin Synthase, but Slightly by Phytochelatins

2020 ◽  
Vol 21 (5) ◽  
pp. 1583 ◽  
Author(s):  
Erika Bellini ◽  
Viviana Maresca ◽  
Camilla Betti ◽  
Monica Ruffini Castiglione ◽  
Debora Fontanini ◽  
...  
Keyword(s):  
High Performance ◽  
Laser Scanning ◽  
Glutathione Transferase ◽  
Cadmium Stress ◽  
Laser Scanning Microscopy ◽  
Cellular Damage ◽  
Confocal Laser ◽  
Peptidase Activity ◽  
Phytochelatin Synthase ◽  
Glutathione S Transferase

In the present work, we investigated the response to Cd in Leptodictyum riparium, a cosmopolitan moss (Bryophyta) that can accumulate higher amounts of metals than other plants, even angiosperms, with absence or slight apparent damage. High-performance liquid chromatography followed by electrospray ionization tandem mass spectrometry of extracts from L. riparium gametophytes, exposed to 0, 36 and 360 µM Cd for 7 days, revealed the presence of γ-glutamylcysteine (γ-EC), reduced glutathione (GSH), and traces of phytochelatins. The increase in Cd concentrations progressively augmented reactive oxygen species levels, with activation of both antioxidant (catalase and superoxide dismutase) and detoxifying (glutathione-S-transferase) enzymes. After Cd treatment, cytosolic and vacuolar localization of thiol peptides was performed by means of the fluorescent dye monochlorobimane and subsequent observation with confocal laser scanning microscopy. The cytosolic fluorescence observed with the highest Cd concentrations was also consistent with the formation of γ-EC-bimane in the cytosol, possibly catalyzed by the peptidase activity of the L. riparium phytochelatin synthase. On the whole, activation of phytochelatin synthase and glutathione-S-transferase, but minimally phytochelatin synthesis, play a role to counteract Cd toxicity in L. riparium, in this manner minimizing the cellular damage caused by the metal. This study strengthens previous investigations on the L. riparium ability to efficiently hinder metal pollution, hinting at a potential use for biomonitoring and phytoremediation purposes.

scholarly journals Fluorescence Microscopy of Superplasticizers in Cementitious Systems: Applications and Challenges

Materials ◽  
2020 ◽  
Vol 13 (17) ◽  
pp. 3733
Author(s):  
Johannes Arend ◽  
Alexander Wetzel ◽  
Bernhard Middendorf
Keyword(s):  
Fluorescence Microscopy ◽  
High Performance ◽  
Laser Scanning ◽  
Laser Scanning Microscopy ◽  
Chemical System ◽  
Confocal Laser ◽  
Granulated Blast Furnace Slag ◽  
Adsorption Analysis ◽  
Resolution Imaging

In addition to the desired plasticizing effect, superplasticizers used in high and ultra-high performance concretes (UHPC) influence the chemical system of the pastes and for example retardation of the cement hydration occurs. Thus, superplasticizers have to be chosen wisely for every material composition and application. To investigate the essential adsorption of these polymers to particle surfaces in-situ to overcome several practical challenges of superplasticizer research, fluorescence microscopy is useful. In order to make the superplasticizer polymers visible for this microscopic approach, they are stained with fluorescence dyes prior the experiment. In this work, the application of this method in terms of retardation and rheological properties of sample systems is presented. The hydration of tricalcium oxy silicate (C3S) in combination with different polycarboxylate ether superplasticizers is observed by fluorescence microscopy and calorimetry. Both methods can identify the retarding effect, depending on the superplasticizer’s chemical composition. On the other hand, the influence of the superplasticizers on the slump of a ground granulated blast furnace slag/cement paste is correlated to fluorescence microscopic adsorption results. The prediction of the efficiency by microscopic adsorption analysis succeeds roughly. At last, the possibility of high-resolution imaging via confocal laser scanning microscopy is presented, which enables the detection of early hydrates and their interaction with the superplasticizers.

scholarly journals Effect of reciprocating instrumentation on chlorhexidine substantivity on human dentin: chemical analysis followed by confocal laser microscopy

2021 ◽  
Vol 37 ◽  
pp. e37038
Author(s):  
Matheus Albino Souza ◽  
Fernanda Duda Bonatto ◽  
Afonso Cristiano Fleck da Silva ◽  
Ezequiel Santin Gabrielli ◽  
Felipe Trentin Motter ◽  
...  
Keyword(s):  
High Performance ◽  
Laser Scanning ◽  
Ex Vivo ◽  
Experimental Tests ◽  
Laser Scanning Microscopy ◽  
Live Cells ◽  
Confocal Laser ◽  
Water Control ◽  
Human Teeth ◽  
Root Canals

The present research analyzed the reciprocating instrumentation associated to chlorhexidine (CHX) substantivity as its correlation with E. faecalis viability in ex vivo root canals. Eighty extracted single-rooted human teeth were used, being 40 to high-performance liquid chromatography (HPLC) and 40 to confocal laser scanning microscopy (CLSM). In both, teeth were decoronated and the cervical third was prepared. In the CLSM analysis, the root canals were inoculated with E. faecalis for 14 days. Samples were divided into 4 groups (n=10) according to instrumentation technique: no instrumentation and irrigation with distilled water (control); manual instrumentation (K-File); rotary instrumentation (ProTaper Next); and reciprocating instrumentation (Reciproc R25). Two percent chlorhexidine was applied as irrigating substance in experimental groups. Longitudinal grooves resulted in 2 halves root and 20 proof bodies in each group. Samples were divided by chance in two groups (n=10) and the outcomes were evaluated after two days and one week. The retained chlorhexidine and live cells after instrumentation techniques in each evaluation time was measured by HPLC and CLSM, respectively. Specific analysis was applied for experimental tests (p≤0.05). Both rotary as well as reciprocating techniques significantly reduced the amount of chlorhexidine on dentin in all observation periods (p<0.05). After evaluation times, all experimental groups presented lower live cells compared to control, but without statistically difference. Intragroup comparisons in times of evaluation showed no differences in instrumentation techniques, in chlorhexidine retention and number of live cells (p>0.05). Reciprocating instrumentation does not interfere on chlorhexidine substantivity.

scholarly journals Confocal Microscopy Predicts the Risk of Recurrence and Malignant Transformation of Mucocutaneous Neurofibromas in NF-1: An Observational Study

2018 ◽  
Vol 2018 ◽  
pp. 1-5
Author(s):  
Giuseppe Giudice ◽  
Giorgio Favia ◽  
Angela Tempesta ◽  
Luisa Limongelli ◽  
Michelangelo Vestita
Keyword(s):  
Laser Scanning ◽  
Low Cost ◽  
Surgical Excision ◽  
Neurofibromatosis Type ◽  
Optical Microscope ◽  
Laser Scanning Microscopy ◽  
Depth Of Field ◽  
Confocal Laser ◽  
Malignant Degeneration ◽  
Risk Of Recurrence

From 2005 to 2010, 20 consecutive patients with fully manifested neurofibromatosis type 1 (NF1) underwent elective neurofibroma resection at our institution (Departments of Plastic Surgery and of Odontostomatology). Specimens were photographed under optical microscope and confocal laser scanning microscopy (CLSM) with ultra-high accuracy of detail, including depth of field. Patients were followed up for a minimum of 4 years and up to a maximum of 12 years, postsurgery. While all nonrecurring lesions showed intense fluorescence, six of the seven lesions with absence of fluorescence under CLSM recurred at a mean of 5.5 years after surgical excision. Among the re-excised lesions, 3 were diagnosed as malignant at the subsequent removal. Despite the limitation of a small cohort, CLSM appears to be a simple and low-cost technique to differentiate forms of neurofibromas with low and high risk of recurrence and malignant degeneration.

Micromorphology of seeds of three Mexican species of Pinguicula (Lentibulariaceae) show autofluorescence using confocal laser scanning microscopy

Phytotaxa ◽  
2021 ◽  
Vol 489 (2) ◽  
pp. 191-199
Author(s):  
JULIÁN HERNÁNDEZ-RENDÓN ◽  
PAOLA FLORES-RODRÍGUEZ ◽  
IGNACIO VILLANUEVA-FIERRO ◽  
ARTURO CASTRO-CASTRO ◽  
EDUARDO RUIZ-SANCHEZ
Keyword(s):  
Confocal Laser Scanning Microscopy ◽  
Laser Scanning ◽  
Low Cost ◽  
Practical Method ◽  
Morphological Characters ◽  
Laser Scanning Microscopy ◽  
Confocal Laser Scanning ◽  
Scanning Microscopy ◽  
Confocal Laser ◽  
Seed Micromorphology

Pinguicula (Lentibulariaceae) includes around 110 described species, 51 of which are distributed in Mexico. Recently, P. casperi was described and its authors observed that it has intermediate morphological characters between P. oblongiloba and P. parvifolia, its putative parents. The micromorphological study of seeds has previously defined the identity of some species, and this can provide evidence of hybridization. The objectives of the present study were 1) to describe the seed micromorphology of P. casperi, P. oblongiloba and P. parvifolia and 2) to provide an economical, easy, and practical method for observing seed micromorphology using confocal laser scanning microscopy (CLSM). Seed micromorphology was considerably different between P. casperi, P. oblongiloba, and P. parvifolia. Therefore, no evidence of hybridization was found. The seeds of the three species were autofluorescent. The process of obtaining the images of the seeds with CLSM was harmless, low cost, and practical. Additionally, the images obtained were high quality and had a high resolution, making it possible to observe and compare the microstructures on the seed surfaces. CLSM is a useful, practical alternative for future taxonomic studies of the genus Pinguicula.

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