scholarly journals Evaluation of the in vitro growth of perolera pineapple (Ananas comosus) explants using organogenesis technique

2021 ◽  
Vol 7 (2) ◽  
pp. 32-39
Author(s):  
Christian Andrei Chacin Zambrano ◽  
Yessica Paola Quintero Avila ◽  
Leydy Gabriela Rodríguez González ◽  
Johanna Alexandra Gómez Santos
Keyword(s):  
Tween 80 ◽  
Ananas Comosus ◽  
Contamination Rate ◽  
In Vitro Growth ◽  
Extension Services ◽  
New Varieties ◽  
Technical Extension ◽  
Establishment Phase ◽  
Economic Standpoint

From an economic standpoint, pineapple (Ananas comosus) is one of the most important fruits in Colombia. A decade ago, the Perolera variety was the most cropped cultivar of the Santander department, however, the variety has been displaced considerably due to the lack of technical extension services and theintroduction of new varieties. This research project was carried out with the intention to conserve the speciesthrough the development of in vitro pineapple explants using the organogenesis technique. Meristemsthat have been extracted from the crown of the Perolera pineapple variety were used for this purpose. Four disinfectant treatments were evaluated by looking at the different kinds of disinfectant exposure times.The treatment that gave the best results in terms of contaminant-free explants was the T2: Commercialdetergent + Tween 80 for 8 minutes, ethyl alcohol at 70% for 1 minute and sodium hypochlorite at 1,5% over10 minutes, with a contamination rate of 7% and 93% of the explants thriving. For the establishment phase,it was found that the medium MS MEP1 with 100% solid salts supplemented with 2000 ul/L BAP - 1000 ul/L ANA - 1000 ul/L AIA and 500 ul/L thiamine enabled 90 % of the pineapple explants to continue developing four weeks after planting. Similarly, the medium containing 3000 ul/L of BAP for the multiplication phase permitted an average proliferation of 4.62 shoots with 9.12 leaves per shoot and a length of 2.25 mm.

scholarly journals Glucan Is a Component of the Mycobacterium tuberculosis Surface That Is Expressed In Vitro and In Vivo

2002 ◽  
Vol 70 (5) ◽  
pp. 2566-2575 ◽  
Author(s):  
J. Reid Schwebach ◽  
Aharona Glatman-Freedman ◽  
Leslie Gunther-Cummins ◽  
Zhongdong Dai ◽  
John B. Robbins ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Cell Envelope ◽  
Tween 80 ◽  
Enzyme Linked Immunosorbent Assay ◽  
In Vitro Growth ◽  
Comprehensive Picture ◽  
The Media ◽  
Surface Polysaccharide

ABSTRACT The outermost layer of Mycobacterium tuberculosis is composed primarily of two polysaccharides, glucan (GC) and arabinomannan. To analyze the surface polysaccharide composition of M. tuberculosis, we generated a monoclonal antibody (MAb) that binds M. tuberculosis GC and is known as MAb 24c5. Immunofluorescence and whole-mount immunoelectron microscopy indicated that GC is on the outermost portion of the bacteria. M. tuberculosis strains Erdman and CDC 1551 were analyzed for their ability to bind MAb 24c5 after in vitro growth in media with and without the detergent Tween 80. MAb 24c5 bound to Erdman and CDC 1551 at all culture times with only slightly greater apparent affinity after extended culture in the absence of Tween 80, indicating that a stable amount of GC polysaccharide antigen is associated with the cell surface of M. tuberculosis. An enzyme-linked immunosorbent assay indicated that GC is antigenically similar to glycogen, and the amount of GC antigen increased in the media of M. tuberculosis cultures grown either with or without the detergent Tween 80. Other nontuberculosis mycobacteria have antigenically similar GCs on their surfaces after in vitro growth. Inoculation of mice with live bacilli but not inoculation with dead bacilli elicited a strong antibody response to GC consistent with production of this antigen in vivo. Our results provide a more comprehensive picture of the M. tuberculosis cell envelope and the conditions that allow expression of M. tuberculosis GC.

scholarly journals Multiplicación in vitro de piña MD-2 (Ananas comosus (L.) Merril) haciendo uso de fertilizantes comerciales

2015 ◽  
Vol 4 ◽  
pp. 77-88
Author(s):  
Juan Francisco Cuéllar Zometa ◽  
Carlos Eduardo Somoza Vargas
Keyword(s):  
Tween 80 ◽  
Ananas Comosus

Este estudio consistió en la multiplicación in vitro de piña MD-2 (Ananas comosus (L.) Merril) bajo la técnica de cultivo de tejidos vegetales, empleando el medio de cultivo desarrollado por Murashige & Skoog (1962), modificado con 3mg de 6 bencil amino purina por litro de medio, tratamiento uno (TI). El tratamiento anterior ha sido contrastado con otro que se basa en un terreno de cultivo in vitro, a base de fertilizante comercial hidrosoluble, medio de cultivo que se identificó como «UNICAES 2», tratamiento dos (T2); buscando igualar la concentración y disponibilidad de cada uno de los elementos necesarios para el correcto desarrollo de las plantas.Se seccionaron yemas apicales de retoños de la base de plantas madre, y se desinfectaron con hipoclorito de sodio al 5% por 15 minutos, adicionando 3 gotas de tween 80 por cada 100ml de solución desinfectante. Se seccionaron las yemas de aproximadamente 0.5cm de longitud por 0.5cm de diámetro. Estas se inocularon en frascos de vidrio conteniendo 30ml de medio de cultivo con 20 repeticiones por tratamiento. Las yemas se incubaron a 16-8 horas luz-oscuridad; con una temperatura promedio de 28ºC y 232 pie candela de intensidad lumínica, proporcionada por 3 lámparas fluorescentes. Luego de 40 días se compararon las yemas obtenidas en ambos tratamientos, tomando en cuenta su longitud y número de brotes laterales.Producción Agropecuaria y Desarrollo Sostenible, Vol. 4, 2015: 77-88

Effect of in Vivo Oxidized Cellulose on in Vitro Growth of Human Respiratory Mucosa and Sub-mucosa During Endoscopic Skull Base Approaches

2016 ◽  
Vol 77 (S 01) ◽  
Author(s):  
Ezequiel Goldschmidt ◽  
Jorge Rasmussen ◽  
Joseph Chabot ◽  
Monica Loressi ◽  
Marcelo Ielpi ◽  
...  
Keyword(s):  
Skull Base ◽  
Oxidized Cellulose ◽  
In Vitro Growth ◽  
Respiratory Mucosa

Uji aktivitas antijamur ekstrak etanol Eleutherine americana Merr. terhadap Microsporum canis secara in vitro

2019 ◽  
Vol 5 (4A) ◽  
pp. 1497
Author(s):  
Buana Dewanti Wimpi ◽  
Diana Natalia ◽  
Effiana Effiana
Keyword(s):  
Tween 80 ◽  
Microsporum Canis ◽  
Eleutherine Americana

Latar Belakang: Dermatofitosis adalah suatu kondisi penyakit yang ditandai dengan infeksi pada jaringan berkeratin seperti epidermis, rambut dan kuku. Kondisi ini disebabkan oleh sekelompok jamur berfilamen terkait yang dikenal sebagai dermatofita. Bawang dayak (Eleutherine americana Merr.) merupakan tanaman berumbi merah yang mengandung senyawa bioaktif yang memiliki kemampuan menghambat pertumbuhan jamur golongan dermatofita. Metode: Umbi bawang dayak diekstraksi dengan metode maserasi menggunakan pelarut etanol 96%. Uji aktivitas antijamur menggunakan metode difusi cakram Kirby-Bauer dengan 5 variasi konsentrasi yaitu 60%, 30%, 15%, 7,5% dan 3,75%. Kontrol positif yang digunakan adalah itrakonazol 8 µg/disk sedangkan kontrol negatif yang digunakan adalah pelarut Tween 80 sebesar 10%. Hasil: Ekstrak umbi bawang dayak mengandung senyawa metabolit sekunder berupa saponin, kuinon, flavonoid, fenol, tanin, alkaloid, steroid dan triterpenoid. Uji aktivitas antijamur ekstrak etanol umbi bawang dayak dengan metode difusi cakram tidak membentuk zona hambat terhadap pertumbuhan Microsporum canis. Kesimpulan: Ekstrak etanol umbi bawang dayak tidak memiliki aktivitas antijamur terhadap pertumbuhan Microsporum canis.

PERILAKU DISOLUSI KETOPROFEN DAN INDOMETASIN FARNESIL TERSALUT GEL KITOSAN-GOM GUAR

2012 ◽  
Vol 12 (1) ◽  
Author(s):  
Purwantiningsih Sugita ◽  
Bambang Srijanto ◽  
Budi Arifin ◽  
Fithri Amelia ◽  
Mahdi Mubarok
Keyword(s):  
Room Temperature ◽  
Guar Gum ◽  
Tween 80 ◽  
Chitosan Solution ◽  
In Vitro Dissolution ◽  
Dissolution Profile ◽  
Magnetic Stirrer ◽  
Dissolution Behaviour ◽  
Shrimp Shell Waste

Chitosan, a modification of shrimp-shell waste, has been utilized as microcapsule. However, it’s fragile gel property needs to be strengthened by adding glutaraldehyde (glu) and natural hydrocolloid guar gum (gg). This research’s purposes were to study dissolution behaviour of ketoprofen and infar through optimum chitosan-guar gum microcapsule. Into 228.6 mL of 1.75% (w/v) chitosan solution in 1% (v/v) acetic acid,38.1 mL of gg solution was added with concentration variation of 0.35, 0.55, and 0.75% (w/v) for ketoprofen microcapsules and 0.05, 0.19, and 0.33% (w/v) for infar microcapsules, and stirred with magnetic stirrer until homogenous. Afterwards, 7.62mL of glu was added slowly under stirring, with concentrations varied: 3, 3.5, and 4% (v/v) for ketoprofen microcapsules, and 4, 4.5, and 5% (v/v) for infar microcapsules. All mixtures were shaked for 20 minutes for homogenization. All mixtures wereshaked for 20 minutes for homogenization. Into each  microcapsule mixture for ketoprofen, a solution of 2 g of ketoprofen in 250 mL of 96% ethanol was added, whereas solution of 100 mg of in 250 mL of 96% ethanol was added into each microcapsule mixture for infar. Every mixture was then added with 5 mL of 2% Tween-80 and stirred with magnetic stirrer for an hour at room temperature. Everymixture was then added with 5 mL of 2% Tween-80 and stirred with magnetic stirrer for an hour at room temperature. Conversion of suspension into fine powders/granules (microcapsules) was done by using spray dryer. The data of [gg], [glu], and medicine’s content from each microcapsule were treated with Minitab 14 software to obtain optimum [gg] and [glu] for microencapsulation. The dissolution behaviour of optimum ketoprofen and infar microcapsules were investigated. The result of optimization by using Minitab Release 14 software showed that among the microcapsule compositions of [gg] and [glu] were 0.35% (w/v) and 3.75% (v/v), respectively, optimum to coat ketoprofen, whereas [gg] and [glu] of 0.05% (w/v) and4.00% (v/v), respectively, optimum to coat infar, at constant chitosan concentration (1.75% [w/v]). In vitro dissolution profile showed that chitosan-guar gum gel microcapsule was more resistant in intestinal pH condition (rather basic) compared with that in gastric pH (very acidic).

Investigating Non-Therapeutic Pharmaceutical Substances for Improving In-Vitro Efficacy of Clindamycin Phosphate Against MRSA and Staphylococcus Epidermidis

2020 ◽  
Vol 18 (2) ◽  
pp. 63-72
Author(s):  
Mohd Aftab Alam ◽  
Fahad I. Al-Jenoobi ◽  
Khaled A. Alzahrani ◽  
Mohammad H. Al-Agamy ◽  
Abdullah M. Al-Mohizea
Keyword(s):  
Fusidic Acid ◽  
Staphylococcus Epidermidis ◽  
Sorbic Acid ◽  
Tween 80 ◽  
Lauryl Sulfate ◽  
Individual Substance ◽  
Dependent Effect ◽  
Pharmaceutical Excipients ◽  
Active Substances

The aim of present study was to investigate the effect of pharmaceutical excipients and other active substances on antimicrobial efficacy of standard antibiotic against resistant and susceptible microorganisms. Pharmaceutical excipients (sodium lauryl sulfate [SLS], Tween-80, citric acid, NaOH, NaCl) and active substances (fusidic acid, sorbic acid) were investigated to check in-vitro efficacy and their effect on the efficacy of standard antibiotic. Clindamycin was selected as standard antibiotic. Clindamycin was found to be ineffective against methicillin-resistant Staphylococcus aureus (MRSA). Fusidic acid and SLS showed concentration dependent effect against MRSA. Other tested substances were also ineffective against MRSA, and also failed to improve the susceptibility of MRSA towards clindamycin. The clindamycin + fusidic acid (0.05 µg, 0.1 µg), and clindamycin + SLS (0.5 mg, 1 mg) showed concentration dependent effect on Staphylococcus epidermidis (S. epidermidis). Clindamycin combinations with fusidic acid or SLS showed better inhibition of S. epidermidis, than individual substance. At lower concentration of clindamycin (2 µg), the sorbic acid (25 µg) improves its effectiveness. SLS (0.5 mg, 1 mg) and clindamycin (4 µg, 10 µg) showed almost equal zone of inhibition against S. epidermidis, respectively. Present findings showed that certain pharmaceutical excipients (e.g. SLS) are effective against resistant and susceptible microbes, and suggested that more excipients should be screened for their antimicrobial potential and their ability to improve the efficacy of standard antibiotics.

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