scholarly journals PARTIAL CLONING AND IDENTIFICATION OF TERPENE SYNTHASE-6 GENE (TPS-6) IN AN AROMATIC PLANT Origanum onites L.

2017 ◽  
Author(s):  
İsmail Poyraz ◽  
Merve Sağlam
Keyword(s):  
Terpene Synthase ◽  
Aromatic Plant ◽  
Origanum Onites

Isolation of Quality Total RNA from the Aromatic Plant Origanum onites

2010 ◽  
Vol 65 (3-4) ◽  
pp. 266-270 ◽  
Author(s):  
Īsmail Poyraz ◽  
Emel Sözen ◽  
Muhittin Arslanyolu
Keyword(s):  
Phenolic Compounds ◽  
Medicinal Plants ◽  
Reverse Transcription ◽  
Extraction Method ◽  
Room Temperature ◽  
Aromatic Plant ◽  
Guanidine Isothiocyanate ◽  
Total Rna ◽  
Root Tissues ◽  
Origanum Onites

We successfully used the guanidine isothiocyanate method for isolation of total RNA from leaf, stem, and root tissues of the aromatic plant Origanum onites. The RNA was extracted with TRI Reagent® at room temperature and was recovered by isopropanol precipitation. The isolated RNA was capable of reverse transcription. The extraction method described here does not require ultracentrifugation, and it is fast, simple, and effective. The procedure can be completed within 3 hours and may be applicable to other aromatic medicinal plants containing high amounts of phenolic compounds.

Kandungan Fenol, Flavonoid dan Terpenoid Ekstrak Metanol Daun Akway (Drimys piperita Hook f.)

Agrotek ◽  
2018 ◽  
Vol 2 (1) ◽  
Author(s):  
Gino Nemesio Cepeda ◽  
Bimo Budi Santoso ◽  
Meike Meilan Lisangan
Keyword(s):  
Human Body ◽  
Total Phenol ◽  
Medical Purpose ◽  
Aromatic Plant ◽  
Physiological Action

Akway (Drimys piperita Hook f.) is the woody, evergreen and aromatic plant that is member of winteraceae family. This plant is used for medical purpose by Sougb tribe lived in Sururey village, Manokwari District. The medical value of the plant lies in some phytochemical substances that produce a definite physiological action on human body. The objective of the research was to know phenol, flavonoid and terpenoid content of akway leaves extracted by methanol. The results indicated that extract methanol of akway leaves contains total phenol 186.40 mg/g and flavonoid 70 mg/g. The main terpenoid compound composed extract was caryophyllene in concentration of 37.09%.

scholarly journals Nanovesicles Loaded with Origanum onites and Satureja thymbra Essential Oils and Their Activity against Food-Borne Pathogens and Spoilage Microorganisms

Molecules ◽  
2021 ◽  
Vol 26 (8) ◽  
pp. 2124
Author(s):  
Giulia Vanti ◽  
Ekaterina-Michaela Tomou ◽  
Dejan Stojković ◽  
Ana Ćirić ◽  
Anna Rita Bilia ◽  
...  
Keyword(s):  
Essential Oils ◽  
Propylene Glycol ◽  
Antimicrobial Properties ◽  
Spherical Shape ◽  
Food Borne Pathogens ◽  
Food Borne ◽  
Spoilage Microorganisms ◽  
Satureja Thymbra ◽  
Origanum Onites

Food poisoning is a common cause of illness and death in developing countries. Essential oils (EOs) could be effective and safe natural preservatives to prevent and control bacterial contamination of foods. However, their high sensitivity and strong flavor limit their application and biological effectiveness. The aim of this study was firstly the chemical analysis and the antimicrobial evaluation of the EOs of Origanum onites L. and Satureja thymbra L. obtained from Symi island (Greece), and, secondly, the formulation of propylene glycol-nanovesicles loaded with these EOs to improve their antimicrobial properties. The EOs were analyzed by GC-MS and their chemical contents are presented herein. Different nanovesicles were formulated with small average sizes, high homogeneity, and optimal ζ-potential. Microscopic observation confirmed their small and spherical shape. Antibacterial and antifungal activities of the formulated EOs were evaluated against food-borne pathogens and spoilage microorganisms compared to pure EOs. Propylene glycol-nanovesicles loaded with O. onites EO were found to be the most active formulation against all tested strains. Additionally, in vitro studies on the HaCaT cell line showed that nanovesicles encapsulated with EOs had no toxic effect. The present study revealed that both EOs can be used as alternative sanitizers and preservatives in the food industry, and that their formulation in nanovesicles can provide a suitable approach as food-grade delivery system.

Decalepis salicifolia (Bedd. ex Hook. f.) Venter: A steno-endemic and critically endangered medicinal and aromatic plant from Western Ghats, India

2021 ◽  
Vol 46 (2) ◽  
Author(s):  
Vereena Rodrigues ◽  
Amit Kumar ◽  
Sivaraman Gokul ◽  
Ashutosh K. Shukla ◽  
Kaliamoorthy Ravikumar ◽  
...  
Keyword(s):  
Western Ghats ◽  
Critically Endangered ◽  
Aromatic Plant

scholarly journals Neue Klasse von Methyltransferasen mit Zyklisierungsaktivität

BIOspektrum ◽  
2021 ◽  
Vol 27 (1) ◽  
pp. 31-33
Author(s):  
Birgit Piechulla ◽  
Nancy Magnus ◽  
Marie Chantal Lemfack ◽  
Stephan Von Reuss
Keyword(s):  
Terpene Synthase ◽  
Alternative Route ◽  
The Novel ◽  
Serratia Plymuthica ◽  
Volatile Metabolites

AbstractMicroorganisms release small volatile metabolites with unique structures, e. g. the polymethylated homosesquiterpene sodorifen from Serratia plymuthica. Two unusual enzymes with novel features are involved in its biosynthesis, a C-methyltransferase with cyclization activity and a terpene synthase that accepts a non-canonical monocyclic C16 substrate. The novel class of methyltransferases represents an alternative route that enlarges terpene diversity.

Essential oils and quality composts sourced by recycling vegetable residues from the aromatic plant supply chain

2021 ◽  
Vol 162 ◽  
pp. 113255 ◽  
Author(s):  
Massimo Zaccardelli ◽  
Graziana Roscigno ◽  
Catello Pane ◽  
Giuseppe Celano ◽  
Marisa Di Matteo ◽  
...  
Keyword(s):  
Supply Chain ◽  
Essential Oils ◽  
Aromatic Plant

scholarly journals Genome-Wide Analysis of Terpene Synthase Gene Family in Mentha longifolia and Catalytic Activity Analysis of a Single Terpene Synthase

Genes ◽  
2021 ◽  
Vol 12 (4) ◽  
pp. 518
Author(s):  
Zequn Chen ◽  
Xiwu Qi ◽  
Xu Yu ◽  
Ying Zheng ◽  
Zhiqi Liu ◽  
...  
Keyword(s):  
Catalytic Activity ◽  
Essential Oils ◽  
Gene Family ◽  
Genome Sequence ◽  
Sequence Assembly ◽  
Terpene Synthase ◽  
Genome Sequence Assembly ◽  
Mentha Longifolia ◽  
Specific Expansion ◽  
Species Specific

Terpenoids are a wide variety of natural products and terpene synthase (TPS) plays a key role in the biosynthesis of terpenoids. Mentha plants are rich in essential oils, whose main components are terpenoids, and their biosynthetic pathways have been basically elucidated. However, there is a lack of systematic identification and study of TPS in Mentha plants. In this work, we genome-widely identified and analyzed the TPS gene family in Mentha longifolia, a model plant for functional genomic research in the genus Mentha. A total of 63 TPS genes were identified in the M. longifolia genome sequence assembly, which could be divided into six subfamilies. The TPS-b subfamily had the largest number of genes, which might be related to the abundant monoterpenoids in Mentha plants. The TPS-e subfamily had 18 members and showed a significant species-specific expansion compared with other sequenced Lamiaceae plant species. The 63 TPS genes could be mapped to nine scaffolds of the M. longifolia genome sequence assembly and the distribution of these genes is uneven. Tandem duplicates and fragment duplicates contributed greatly to the increase in the number of TPS genes in M. longifolia. The conserved motifs (RR(X)8W, NSE/DTE, RXR, and DDXXD) were analyzed in M. longifolia TPSs, and significant differentiation was found between different subfamilies. Adaptive evolution analysis showed that M. longifolia TPSs were subjected to purifying selection after the species-specific expansion, and some amino acid residues under positive selection were identified. Furthermore, we also cloned and analyzed the catalytic activity of a single terpene synthase, MlongTPS29, which belongs to the TPS-b subfamily. MlongTPS29 could encode a limonene synthase and catalyze the biosynthesis of limonene, an important precursor of essential oils from the genus Mentha. This study provides useful information for the biosynthesis of terpenoids in the genus Mentha.

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