scholarly journals Antioxidant activity of heterocyclic amino containing derivatives of naphthoquinone and their compositions with surface-active rhamnolipids

2021 ◽  
Vol 4 (1) ◽  
pp. 109-115
Author(s):  
N. V. Polish ◽  
◽  
N. G. Marintsova ◽  
A. I. Karkhut ◽  
O. S. Yaremkevysh ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Activity ◽  
Oxidative Modification ◽  
Heterocyclic Amine ◽  
Oxidative Modification Of Proteins ◽  
High Antioxidant Activity ◽  
Surface Active ◽  
Derivatives Of

Composite preparations based on heterocyclic amine-containing naphthoquinone derivatives and surfactant rhamnolipids were obtained. The intensity of the processes of lipid peroxidation (LPO), oxidative modification of proteins (OMB) and radical-absorbing activity against 1,1-diphenyl-2-picrylhydrazyl (DFPG) was studied. Compounds exhibiting high antioxidant activity against LPO and OMB processes were identified, namely: 2-[(6-(4-fluorophenyl-5-oxo-2,5-dihydro-1,2,4-triazin-3-yl) phenyl)amino]naphthalene-1,4-dione 1d and its composite preparation with rhamnolipid 1d + RL. It was found that all synthesized compounds show antioxidant activity in LPO processes.

The evaluation of lipid peroxidation and oxidative modification of proteins in blood serum under obesity development and the consumption of aqueous kidney beans Phaseolus vulgaris pods extract

2020 ◽  
Vol 33 (1) ◽  
pp. 38-44
Author(s):  
Alona Yurchenko ◽  
Daryna Krenytska ◽  
Olexii Savchuk ◽  
Tetiana Halenova ◽  
Natalia Raksha ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Oxidative Modification ◽  
Resistance Development ◽  
Oxidative Modification Of Proteins ◽  
Kidney Beans ◽  
Calorie Diet ◽  
Diet Control ◽  
High Calorie Diet ◽  
High Calorie

AbstractOur interest has focused on the investigation of the anti-obese potential of kidney beans (P. vulgaris) pods extract. In the course of the study, obesity development in rats was induced with high-calorie diet. Control and obese rats then have consumed with aqueous kidney beans (P. vulgaris) pods extract during 6 weeks (200 mg/kg). Results show that the long-term consumption of P. vulgaris pods extract can lead to the reduction of hyperglycemia and insulin resistance development. Furthermore, we saw a normalization of lipid peroxidation parameters and oxidative modification of protein due to the consumption of the kidney beans (P. vulgaris) pods extract. Our experimental data demonstrate the ability of the kidney beans (P. vulgaris) pod extracts to mitigate obesity development but the details of this mechanism remains to be not fully understood.

scholarly journals OXIDATIVE MODIFICATION OF PROTEINS AND GLUTATHIONE SYSTEM IN ADIPOCYTES UNDER DIABETES

2014 ◽  
Vol 13 (3) ◽  
pp. 84-90 ◽  
Author(s):  
Ye. V. Shakhristova ◽  
Ye. A. Stepovaya ◽  
V. V. Ivanov ◽  
O. L. Nosareva ◽  
N. V. Ryazantseva ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Glutathione Peroxidase ◽  
Alloxan Diabetes ◽  
Antioxidant Defense ◽  
Oxidative Modification ◽  
Glutathione System ◽  
Glutathione Peroxidase Activity ◽  
Oxidative Modification Of Proteins ◽  
Carbonyl Derivatives ◽  
Derivatives Of

Currently, diabetes ranks third in relation to medical and social significance after cardiovascular diseases and cancer and is the leading cause of blindness; it greatly increases the risk of myocardial infarction, coronary heart disease, nephropathy and hypertension in patients with this disorder; therefore clinical and experimental studies aimed at investigation of diabetes emergence and development mechanisms are urgent.The aim of the study was to investigate the status of oxidative modification of proteins and glutathionedependent antioxidant defense system in adipocytes of rats with alloxan diabetes under conditions of oxidative stress.Material and methods. Development of type 1 diabetes was induced in rats by alloxan administration (90 mg/kg of body mass). Adipocytes were obtained from epididymal adipose tissue of rats. The level of carbonyl derivatives of proteins, oxidized tryptophan, bityrosine, general, reduced, oxygenated and protein-bound glutathione, as well as glutathione peroxidase activity in adipocytes of rats was determined.Results. In adipocytes of rats with alloxan diabetes, concentration of carbonyl derivatives of proteins, bityrosine and oxidized tryptophan increased on the background of redox-potential of glutathione system and glutathione peroxidase activity decrease.Conclusion. The obtained data indicate the activation of free-radical oxidation of proteins and reduction of antioxidant defense under conditions of oxidative stress in the adipose tissue of rats with alloxan diabetes; this process plays an important role in pathogenesis of diabetes and its complications development.

scholarly journals Correction of redox metabolism and the relationship of markers of systemic chronic inflammation with a measure of overall survival in cancer patients with multiorgan resections

2021 ◽  
Vol 17 (4) ◽  
pp. 74-79
Author(s):  
Ye.V. Shulga
Keyword(s):  
Lipid Peroxidation ◽  
Overall Survival ◽  
Intensive Care ◽  
Cancer Patients ◽  
Tumor Necrosis ◽  
Oxidative Modification ◽  
Necrosis Factor Alpha ◽  
Oxidative Modification Of Proteins ◽  
Factor Alpha ◽  
Necrosis Factor

Background. According to the literature, multiorgan resections are carried out with the most radical removal of healthy tissue. Therefore, there is a need for the most favorable preparation of the patient with perioperative therapy to reduce further complications with obtaining improved clinical and pathological prognostic indicators to increase the overall survival. Purpose: correction of redox metabolism based on the data of the oxidative modification of proteins, degree of their catabolic reaction, and data on the enzymatic state and lipid peroxidation in cancer patients with multiorgan resections on the gastrointestinal tract under conditions of perioperative intensive care. Materials and methods. The study involved 117 cancer patients aged 67.6 ± 3.7 years with locally advanced tumor processes of the gastrointestinal tract. Studies have been conducted on the enzymatic state, lipid peroxidation and oxidative modification of proteins, both in terms of the degree of fragmentation of their products in spontaneous and iron-induced reactions, and the level of medium-weight protein molecules. Serum levels of circulating tumor necrosis factor alpha, interleukin-6 and -8 were determined. Survival analysis was performed using the Kaplan-Meier method. Results. The study of patients has shown an improvement in the indicators of oxidative modification of proteins and lipid peroxidation. Survival in cancer patients with multiorgan resections demonstrated dependence on the levels of tumor necrosis factor alpha, interleukin-6 and -8 in the blood serum. Conclusions. Conducting perioperative intensive care makes it possible to reliably restore indicators of the enzymatic state, lipid peroxidation and oxidative protein modification products (on the fourth day and second week, p < 0.05). This gives us grounds for recommending the determination of these parameters in the group of cancer patients and the implementation of metabolic intensive care. In the group of L-ornithine L-aspartate and quercetin combination with povidone, the lowest indicators of systemic chronic inflammation and the best overall survival were found.

scholarly journals Oxidative metabolism of neutrophils in patients with community-acquired pneumonia

2016 ◽  
Vol 29 (1) ◽  
pp. 5-7
Author(s):  
Lyudmila Andreevna Demidchik ◽  
Valentina Vitalyevna Lee ◽  
Larissa Yevgenyevna Muravlyova ◽  
Vilen Borisovich Molotov-Luchanskiy ◽  
Ryszhan Yemelyevna Bakirova ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Advanced Oxidation ◽  
Community Acquired Pneumonia ◽  
Oxidative Modification ◽  
Control Group ◽  
Advanced Oxidation Protein Products ◽  
Oxidative Modification Of Proteins ◽  
Moderate Severity ◽  
Carbonyl Derivatives ◽  
Derivatives Of

Abstract At the present time, available views show our limited knowledge of the peculiarities of the functional status of neutrophils and their metabolism in patients with community-acquired pneumonia (CAP). The studying of changes of metabolic status of neutrophils can broaden our views about pneumonia pathogenesis and define datum points of therapeutic effect. Purpose of our research: to define oxidative stress activity and the level of oxidative modification of proteins of neutrophils in CAP patients. Materials and methods: neutrophils obtained from 23 patients with community-acquired pneumonia. Control group consisting of 19 healthy volunteers. The reactive carbonyl derivatives of proteins and advanced oxidation protein products were defined so as to assess the oxidative damage of proteins. The malondialdehyde and nitrite ions were assessed as being indicators of the oxidative stress. The neutrophils of CAP patients with moderate severity were characterized by a tendency of evidencing decreasing content of advanced oxidation protein products, along with the statistically important enhanced levels of carbonyl derivatives and nitrite ions, while their malondialdehyde status practically leveled off with the control and had only an insignificant trend towards growth. We have demonstrated the accumulation of carbonyl derivatives and nitrite ions in the peripheral neutrophils of CAP patients. These results give evidence of an oxidative misbalance in the cells which contributes to the aggravation of the disease.

Indices of peroxidation of proteins, lipids and antioxidant system as the markers of endogenous intoxication in patients with syndrome of obstructive sleep apnea

2018 ◽  
pp. 4-12
Author(s):  
Yuriy Minin ◽  
Tetiana Kucherenko ◽  
Julia Burlaka ◽  
Julia Klys ◽  
Natalya Voroshilova ◽  
...  
Keyword(s):  
Obstructive Sleep Apnea ◽  
Sleep Apnea ◽  
Blood Serum ◽  
Antioxidant System ◽  
Oxidative Modification ◽  
Oxidative Modification Of Proteins ◽  
Endogenous Intoxication ◽  
Obstructive Sleep ◽  
Derivatives Of ◽  
Medium Weight

The syndrome of obstructive sleep apnea (OSAS) is accompanied by episodes of hypoxia and reoxygenation, which causes an increase in the level of active forms of free radicals that lead to the development of the oxidative stress. The aim of this work was to investigate the intensity of peroxidation of proteins and lipids, the components of the antioxidant system and the biochemical parameters endogenous intoxication in patients with OSAS. Materials and methods: Twenty patients with OSAS were examined at the SI "Prof. O.S. Kolomyychenko Institute of Otolaryngology NAMS of Ukraine" (Kiev). They were divided into two groups: 10 of them were with apnea sleep index less than 30 episodes of respiratory arrest per hour (IA<30), another 10 ones – with more than 30 episodes (IA>30). The control group was consisted of 10 healthy persons. The object of biochemical studies was blood serum. The intensity of the oxidative modification of blood serum proteins was estimated by 2,4-dinitrophenyl hydrazine reaction by Levin in modification of Reznick. The evaluation of the intensity of peroxide processes was determined by interaction with 2-thiobarbiturac acid (TBA) by the method of Goncharenko. The activity of catalase was determined by the method of Korolyuk et al. The content of free thiol groups (SH-) was determined by interaction with 5,5'-dithio-bis-(2-nitrobenzoic acid) by the method of Sokolovsky et al. The contents of medium weight molecules and tyrosine-containing peptides (TCP) were determined spectrophotometrically at 254 and 280 nm, respectively. Statistical processing of results was carried out using a program package of biometric data WinPEPI. Results: In patients with OSAS whose IA<30, an increase of the level of TCP, oxidative modification of proteins, the content of TBA-positive products and a decrease in the level of SH-groups. In patients with IA>30, increased levels of medium weight molecules, TСP, oxidative modification of proteins, the content of TBA-positive products and the activity of catalase. A significant difference was observed in patients with syndrome of obstructive sleep apnea of varying degrees of severity of the content of aldehyde and ketone derivatives of neutral proteins; aldehyde derivatives of the main nature, the content of TBA-positive products and the activity of catalase. Conclusions: The results of the conducted studies indicate an increase in the intensity of the processes of peroxidation of proteins and lipids, the change in the components of the antioxidant system and the development of endogenous intoxication in patients with OSAS.

Synthesis of Some New 5-Indolylidene-4-thiazolidinone Derivatives of 1,2,4-triazole as Potent Antioxidant and Antifungal Agents

2019 ◽  
Vol 4 (3) ◽  
pp. 174-179
Author(s):  
Takallum Khan ◽  
Ritu Yadav
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Free Radical ◽  
Antifungal Activity ◽  
Antifungal Agents ◽  
Antioxidant Potential ◽  
Ic50 Value ◽  
High Antioxidant Activity ◽  
Dpph Scavenging ◽  
Derivatives Of

A series of 1,2,4-triazolyl-4-(5-indolylidene)-thiazolidinone derivatives were synthesized and screened for their antifungal and antioxidant activity. Among these synthesized compounds 3d, 3g showing good antifungal activity and compounds 3b, 3d, 3f and 3h have high % antioxidant activity with lower IC50 value of 11.21, 20.89, 17.51 and 14.05 respectively. We report the antioxidant potential of the said class of compound in which free radical is generated by methelenic and 2nd carbon of thiazolidinone ring. The antifungal activity reported against A. niger, C. albicans and A. flavus. The antioxidant activity of all the synthesized compounds was screened by H2O2, DPPH scavenging and by phosphomolybdenum method with respect to ascorbic acid.

Correction of Free Radical Blood Imbalance and Behaviour in Prenatally Stressed Rats with α-Tocopherol

2021 ◽  
pp. 25-34
Author(s):  
Olʼga N. Kuleshova ◽  
◽  
David L. Teplyy ◽  
Dmitriy D. Teplyy
Keyword(s):  
Antioxidant Activity ◽  
Superoxide Dismutase ◽  
Free Radical ◽  
Oxidative Modification ◽  
Male Rats ◽  
Total Antioxidant Activity ◽  
Oxidative Modification Of Proteins ◽  
Total Antioxidant ◽  
Prenatally Stressed ◽  
Ceruloplasmin Activity

The research involved female mongrel white rats (n = 16) and their offspring (sexually mature males, n = 75). Pregnant females were divided into 4 groups: control (intact animals), stress (from the 16th to the 19th day of pregnancy females were subjected to 3-hour immobilization in plastic cases), stress+α-tocopherol (against the background of immobilization, females received α-tocopherol at a dose of 1 mg per 100 g of weight starting from the 2nd day of pregnancy), and α-tocopherol (females received α-tocopherol in the same dose and regimen as the group above). The control and stress groups received vegetable oil instead of α-tocopherol. In 3-month-old female offspring, we studied behaviour and determined the levels of products of free radical modification of proteins and lipids, total antioxidant activity as well as superoxide dismutase and ceruloplasmin activity in the blood plasma. Prenatal stress increased the exploratory and motor activity in offspring and modified the level of free radical homeostasis in the serum of male rats: there was an increase in the level of products of oxidative modification of proteins, thiobarbituric acid reactive substances concentration, end products of lipid peroxidation (Schiff bases), total antioxidant activity and ceruloplasmin activity, as well as a decrease in superoxide dismutase activity. Alpha-tocopherol taken by females during pregnancy brought all the oxidation parameters modified by immobilization to the values of the control group; the levels of primary products of oxidative modification of proteins and lipid peroxidation decreased two-fold, compared with the stress group. The activity of superoxide dismutase and ceruloplasmin returned to the control values, but the total antioxidant activity remained at the level of the stress group. Thus, there is reason to assume that α-tocopherol has a significant corrective effect on the intensity of free radical processes in prenatally stressed white male rats.

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