scholarly journals Evaluation of The Effect of Pasak Bumi (Eurycoma longifolia Jack) to Increase Androgen Levels

INSIST ◽  
2017 ◽  
Vol 1 (1) ◽  
Author(s):  
Hurip Pratomo
Keyword(s):  
Serum Testosterone ◽  
Female Rats ◽  
Male Rats ◽  
Male Rat ◽  
Testosterone Levels ◽  
Eurycoma Longifolia ◽  
Testosterone Administration ◽  
Eurycoma Longifolia Jack ◽  
Boiled Water ◽  
Androgen Levels

Abstract—Background:The ability of reproductive disorders in men and experimental male rat is characterized among other things by a decrease in androgen such as testosterone levels. Materials of pasak bumi root (Eurycoma longifolia Jack) increased libido behavior of experimental male rat. Libido associated with higher levels of testosterone, it is therefore necessary to evaluate the effect of pasak bumi on androgen levels such as testosterone. Methods: Male rats at 9.00 am treated controls and pasak bumi, then at 14.00 pm tempted with estrus female rats 10 minutes. Then, both control and pasak bumi blood  samples were collected from male rat heart on day 1st  and day 3rd  at 14.15 pm. Measurement of testosterone levels were calculated using radioimmunoassay (RIA). Results: An increase in serum  testosterone levels in the blood on day-3rd  administration pasak bumi boiled water 18 mg/200 g dose  significantly different than the  control within day 1st to 3rd. The average levels of testosterone administration control (boiled water of aquades) day 1st = 0.50 ng/ml, day 3rd = 2.46 ng / ml, increased markedly on pasak bumi treatment day 1st = 4.00 ng / ml and day 3 = 9.73 ng / ml, (Duncan test, α=0.05). Conclusions: Testosterone levels can be increased markedly after the consumption of the pasak bumi boiled water for 3 days.Keywords—Pasak bumi, a dose of  18 mg/200 g, testosterone.

scholarly journals Evaluation of The Effect of Pasak Bumi (Eurycoma longifolia Jack) to Increase Androgen Levels

INSIST ◽  
2016 ◽  
Vol 1 (1) ◽  
Author(s):  
Hurip Pratomo
Keyword(s):  
Serum Testosterone ◽  
Female Rats ◽  
Male Rats ◽  
Male Rat ◽  
Testosterone Levels ◽  
Eurycoma Longifolia ◽  
Testosterone Administration ◽  
Eurycoma Longifolia Jack ◽  
Boiled Water ◽  
Androgen Levels

Abstract—Background:The ability of reproductive disorders in men and experimental male rat is characterized among other things by a decrease in androgen such as testosterone levels. Materials of pasak bumi root (Eurycoma longifolia Jack) increased libido behavior of experimental male rat. Libido associated with higher levels of testosterone, it is therefore necessary to evaluate the effect of pasak bumi on androgen levels such as testosterone. Methods: Male rats at 9.00 am treated controls and pasak bumi, then at 14.00 pm tempted with estrus female rats 10 minutes. Then, both control and pasak bumi blood  samples were collected from male rat heart on day 1st  and day 3rd  at 14.15 pm. Measurement of testosterone levels were calculated using radioimmunoassay (RIA). Results: An increase in serum  testosterone levels in the blood on day-3rd  administration pasak bumi boiled water 18 mg/200 g dose  significantly different than the  control within day 1st to 3rd. The average levels of testosterone administration control (boiled water of aquades) day 1st = 0.50 ng/ml, day 3rd = 2.46 ng / ml, increased markedly on pasak bumi treatment day 1st = 4.00 ng / ml and day 3 = 9.73 ng / ml, (Duncan test, α=0.05). Conclusions: Testosterone levels can be increased markedly after the consumption of the pasak bumi boiled water for 3 days.Keywords—Pasak bumi, a dose of  18 mg/200 g, testosterone.

scholarly journals Commercial Formulation of Chlorpyrifos Alters Neurological Behaviors and Fertility

Biology ◽  
2020 ◽  
Vol 9 (3) ◽  
pp. 49
Author(s):  
Enoka P. Kudavidanage ◽  
D. M. I. Dissanayake ◽  
W. L. Rangi Keerthirathna ◽  
N. Lasni Wathima Nishshanke ◽  
L. Dinithi C. Peiris
Keyword(s):  
Wistar Rats ◽  
Serum Testosterone ◽  
Female Rats ◽  
Male Rats ◽  
Risk Minimization ◽  
Commercial Formulation ◽  
Male Wistar Rats ◽  
Neurological Alterations ◽  
Implantation Sites ◽  
Androgen Levels

Pesticides are known to result in toxic insult. We aimed to evaluate Judo 40, the commercial formulation of chlorpyrifos on the neurological activities, fertility, and hormone levels of male rats. Male Wistar rats were treated orally with 1 mL of 20 or 50 mg/kg Judo 40. The doses were administered four times, twice a day. Sexual and exploratory behavior indices, fertility indices, serum androgen levels, blood acetylcholinesterase (BChE) levels, and neurological and muscular effects were evaluated. Serum testosterone and luteinizing hormone were significantly reduced in the rats receiving 50 mg/kg Judo 40. A reduction in viable implantation sites and live pups born were evident in the female rats mated with the male rats treated with the highest dose. Similarly, in the rats treated with the highest dose of Judo 40, a significant reduction in plasma BChE enzyme was observed. According to the results, prolonged Judo 40 exposure can cause impairment of the neurological alterations and sex hormones leading to impaired fertility. Therefore, chemical handlers should be educated on protection and risk minimization.

scholarly journals Changes in Leptin in Relation to Increased Testosterone Levels Associated with Eurycoma Longifolia Jack (Tongkat Ali) Root Extract Consumption in Male Rats

2017 ◽  
Vol 9 (1) ◽  
pp. 40-42 ◽  
Author(s):  
Ghasak Ghazi Faisal ◽  
Mainul Haque ◽  
Ghazi Faisal Najmuldeen ◽  
Basma Ezzat Al-Ahmad ◽  
Ali Sabri Radeef ◽  
...  
Keyword(s):  
Male Rats ◽  
Root Extract ◽  
Testosterone Levels ◽  
Eurycoma Longifolia ◽  
Eurycoma Longifolia Jack

RELATION BETWEEN CIRCULATING LEVELS OF TESTOSTERONE, LH AND FSH IN INTACT AND CASTRATED, ADULT, MALE RATS AFTER TESTOSTERONE ADMINISTRATION

1975 ◽  
Vol 79 (2) ◽  
pp. 380-386 ◽  
Author(s):  
H. L. Verjans ◽  
H. J. van der Molen ◽  
K. B. Eik-Nes
Keyword(s):  
Adult Male ◽  
Serum Testosterone ◽  
Serum Levels ◽  
Male Rats ◽  
Male Rat ◽  
Normal Male ◽  
Serum Lh ◽  
Adult Male Rat ◽  
Normal Rat ◽  
Testosterone Administration

ABSTRACT Serum levels of LH, FSH and testosterone were measured by radioimmunoassay in intact and castrated, adult, male rats after testosterone was administered subcutaneously for seven days in doses ranging from 25 to 200 μg per 100 g body weight per day. Such treatment increased circulating testosterone both in intact and castrated rats, but its effects on serum gonadotrophins were different in these animal groups. All doses of testosterone suppressed serum LH and FSH in the normal rat. In the castrates, treatment with the lowest dose of testosterone resulted in serum LH levels significantly above the high castrate levels, while serum FSH tended to drop. Administration of the highest doses of testosterone did not depress serum LH and FSH in the castrates to those of intact, normal animals, though serum testosterone in these castrates was much higher than in normal, male rats. It is concluded, that the sensitivity of the hypothalamic-pituitary system for daily, subcutaneous testosterone administration during seven days is not the same in the intact and castrated, adult, male rat and that testicular factors different from testosterone may play a role in regulating production and/or secretion of gonadotrophins by the hypophysis in male animals.

scholarly journals Increased bioactivation of dihaloalkanes in rat liver due to induction of class Theta glutathione S-transferase T1-1

1998 ◽  
Vol 335 (3) ◽  
pp. 619-630 ◽  
Author(s):  
Philip J. SHERRATT ◽  
Margaret M. MANSON ◽  
Anne M. THOMSON ◽  
Erna A. M. HISSINK ◽  
Gordon E. NEAL ◽  
...  
Keyword(s):  
Western Blotting ◽  
Rat Liver ◽  
Female Rats ◽  
Male Rats ◽  
Male Rat ◽  
Female Rat ◽  
Glutathione S Transferase ◽  
Chemopreventive Agents ◽  
Cytoplasmic Staining ◽  
Potent Inducer

A characteristic feature of the class Theta glutathione S-transferase (GST) T1-1 is its ability to activate dichloromethane and dibromoethane by catalysing the formation of mutagenic conjugates. The level of the GSTT1 subunit within tissues is an important determinant of susceptibility to the carcinogenic effects of these dihaloalkanes. In the present study it is demonstrated that hepatic GST activity towards these compounds can be elevated significantly in female and male Fischer-344 rats by feeding these animals on diets supplemented with cancer chemopreventive agents. Immunoblotting experiments showed that increased activity towards the dihaloalkanes is associated with elevated levels of the GSTT1 subunit in rat liver. Sex-specific effects were observed in the induction of GSTT1 protein. Amongst the chemopreventive agents tested, indole-3-carbinol proved to be the most potent inducer of hepatic GSTT1 in male rats (6.2-fold), whereas coumarin was the most potent inducer of this subunit in the livers of female rats (3.5-fold). Phenobarbital showed significant induction of GSTT1 only in male rat liver and had little effect in female rat liver. Western blotting showed that class Alpha, Mu and Pi GST subunits are not co-ordinately induced with GSTT1, indicating that the expression of GSTT1 is determined, at least in part, by mechanisms distinct from those that regulate levels of other transferases. The increase in amount of hepatic GSTT1 protein was also reflected by an increase in the steady-state level of mRNA in response to treatment with chemopreventive agents and model inducers. Immunohistochemical detection of GSTT1 in rat liver supported the Western blotting data, but showed, in addition to cytoplasmic staining, significant nuclear localization of the enzyme in hepatocytes from some treated animals, including those fed on an oltipraz-containing diet. Significantly, the hepatic level of cytochrome P-450 2E1, an enzyme which offers a detoxification pathway for dihaloalkanes, was unchanged by the various inducing agents studied. It is concluded that the induction of GSTT1 by dietary components and its localization within cells are important factors that should be considered when assessing the risk dihaloalkanes pose to human health.

Toxicological evaluation of hydroethanol leaf extract of Pupalia lappacea (Linn.) Juss. (Amaranthaceae) in rodents

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Murtala Akanji Abdullahi ◽  
Elijah Oladapo Oyinloye ◽  
Akinyinka Alabi ◽  
Aderonke Adeyinka Aderinola ◽  
Luqman Opeyemi Ogunjimi ◽  
...  
Keyword(s):  
Leaf Extract ◽  
Density Lipoprotein ◽  
Serum Testosterone ◽  
Female Rats ◽  
Male Rats ◽  
Laboratory Animals ◽  
Serum Testosterone Level ◽  
Control Group ◽  
Toxicological Evaluation ◽  
Liver And Kidney

Abstract Objectives Several studies have established the ethnobotanical benefits of Pupalia lappacea (PL) in laboratory animals without extensive toxicological evaluation of its safety profiles. Thus, an extensive toxicological investigation of sub-chronic oral administration of the hydroethanol leaf extract of P. lappacea in rodents was carried out in this study. Methods Different groups of rats were treated orally with the extract (10, 50 and 250 mg/kg) daily for 90 consecutive days. The control group received distilled water (10 mL/kg). After 90 days, some rats were left for additional 30 days without treatment for reversibility study. Blood and organs samples were collected for different evaluations at the end of study periods. Results The extract decreased the bodyweights, feeding and water intakes in female rats. PL increased the weights of the liver and kidney in male rats. PL increased the red blood cell (RBC), packed cell volume (PCV), hemoglobin (Hb), triglycerides (TRIG), cholesterol and high density lipoprotein (HDL) contents in rats. PL (250 mg/kg) significantly reduced the sperm motility and serum testosterone level. Cyto-architectural distortions of the testes, liver and spleen were visible. Conclusions The findings showed that P. lappacea is relatively safe at lower doses but cautions should be taken at higher dose.

scholarly journals EFFECT OF YOHIMBINE ON CLOMIPRAMINE-INDUCED SEXUAL DYSFUNCTION IN MALE RATS

2017 ◽  
Vol 10 (2) ◽  
pp. 92
Author(s):  
Devangam Sheshadri Shekar
Keyword(s):  
Sexual Behavior ◽  
Sexual Dysfunction ◽  
Histopathological Examination ◽  
Red Light ◽  
Female Rats ◽  
Male Rats ◽  
Male Rat ◽  
Testicular Damage

Object: The present investigation has been carried out to find out the effect of yohimbine on clomipramine-induced sexual dysfunction in male rats.Methods: The male rats were treated with clomipramine and yohimbine simultaneously for 60 days. During the treatment, all the male rats werechallenged with the female rats which are in estrous phase and their sexual behavior was observed under dim red light. Half of the animals in each group and remaining on 60 day were sacrificed, blood was collected and serum separated. Testis was collected and preserved in 10% formalin forsubsequent histopathological examination. thResults: The study reveals that yohimbine failed to antagonize the clomipramine-induced sexual dysfunction in male rats in all aspects, except thepartial improvement in the sexual behavior.Conclusion: Yohimbine a well-known aphrodisiac failed to antagonize the clomipramine-induced sexual dysfunction in male rats. The decrease intestosterone levels, a decrease in spermatozoa count were continued even in the presence of yohimbine except improvement in the sexual behaviorparameters. Hence, yohimbine could not be a safe antidote against clomipramine-induced sexual dysfunction in male rats.Keywords: Yohimbine, Clomipramine, Testosterone, Male rat sexual competence, Testicular damage.

Influence of the environmental temperature on the post-partum testosterone surge in the rat

1987 ◽  
Vol 115 (4) ◽  
pp. 478-482 ◽  
Author(s):  
J. Roffi ◽  
F. Chami ◽  
P. Corbier ◽  
D. A. Edwards
Keyword(s):  
Ambient Temperature ◽  
Environmental Temperature ◽  
Post Partum ◽  
Serum Testosterone ◽  
The Body ◽  
Male Rats ◽  
Effect Of Temperature ◽  
Male Rat ◽  
Foster Mother ◽  
The Central Nervous System

Abstract. In the neonatal male rat, a rapid and transient increase in serum testosterone occurs about 2 h after birth. This post-partum testosterone surge (PPTS) has been implicated in the masculinization and defeminization of the central nervous system. The present study shows that environmental temperature can have a profound influence on the PPTS. Male rats were delivered from their mothers by caesarean section on day 22 of gestation. Immediately thereafter, neonatal males were placed at an ambient temperature of either 18, 21, 24 or 30°C. With 2 h of exposure, the body temperature was in close correspondence with the ambient temperature. The PPTS was clearly abolished in the pups exposed for 2 h at either 18 or 21°C. The effect of temperature was reversible: by placing pups at either 18 or 21°C for 2 h after delivery, and then rewarming by placing them with a foster mother, the PPTS was delayed until 4 h after birth, i.e. 2 h after the beginning of rewarming. Thus, environmental cooling appears to retard the development of neural and/or endocrine systems mediating the PPTS. Aberrant maternal care which would produce substantial cooling of the male pups would be expected to affect the PPTS, which in turn might affect the sexuality of male progeny.

scholarly journals PENGARUH PEMBERIAN EKSTRAK AKAR PASAK BUMI (EURYCOMA LONGIFORA JACK) TERSTANDAR TERHADAP GAMBARAN HISTOPATOLOGIK TESTIS TIKUS WISTAR

2017 ◽  
Vol 13 (3) ◽  
Author(s):  
Condro Suro Miyarso ◽  
Tri Cahyani Widiastuti ◽  
Naelaz Zukhruf Wakhidatul Kiromah
Keyword(s):  
Body Weight ◽  
Testosterone Level ◽  
Positive Control ◽  
Negative Control ◽  
Male Rats ◽  
Histopathological Changes ◽  
Group Iii ◽  
Eurycoma Longifolia ◽  
Group I ◽  
Eurycoma Longifolia Jack

The root of pasak bumi (Eurycoma longifolia, Jack) is one of plant from Indonesia known as aphrodisiac. The study about its standardized extract as aphrodisiac was show increasing libido, testosterone level, FSH and LH on male rats. This study was conducted to evaluate testosterone level and histopathological changes of the testes of male rats of standardized extract of pasak bumi root. There are 50 old male rats which ages 3-4 months old divided to five groups. Group I as negative control was administered aquadestilata. Group II as positive control was administered testosterone (Andriol®). Group III, IV and V ware given standardized extract of pasak bumi root at the dose 50, 100 and 200 mg/kg body weight Respectively. The extract was given orally twice a day for six days and forty nine days and then the testes was taken out on 7th and 50th day. The histopathological of the testes was evaluated using quantitative parameter by the presence of testes weight, number and diameter of Leydig cells. Testosterone level was taken on 7th and 50th day used ELISA method. The results were analyzed using parametric test, one way ANOVA (P<0,05), post hoc Dunnet (2-sided) (P < 0,05). The result of this study shows that the standardized extract of pasak bumi root was not effect on histopathological changes of the testes but it could increased the level of testosterone m at the dose 100 and 200 mg/kg body weight.  Key word : Eurycoma longifolia, Jack, aphrodisiac, histopathological testes testosteron

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