scholarly journals Assessment of the Level of Hazard Analysis and Critical Control Point (HACCP) Prerequisites Adopted in Poultry Meat Production and Processing in Khartoum State, Sudan

2021 ◽  
Vol 3 (5) ◽  
pp. 46-54
Author(s):  
Mona Ahmed Babiker Ahmed ◽  
Atif Elamin Abdelgadir ◽  
Hayfa Mohammed Ismail
Keyword(s):  
Bacterial Growth ◽  
Hazard Analysis ◽  
Control Point ◽  
Poultry Meat ◽  
Meat Production ◽  
P Value ◽  
Gram Negative ◽  
Critical Control Point ◽  
Significant Difference ◽  
Meat Samples

This study was conducted to evaluate the current implementation level of Hazard Analysis and Critical Control Point (HACCP) prerequisites adopted in poultry meat production and processing in Khartoum State, Sudan. To achieve the goal, a cross sectional study was conducted. Data and samples were collected from January to September 2018 from 12 close system broiler slaughterhouses according to Non-Probability Multistage Cluster Sampling Method in Khartoum, Khartoum North (Bahri), and Omdurman localities (4 farms from each). A standardized questionnaire was conducted for slaughterhouses (12 for each) to investigate the current status of implemented HACCP prerequisites. One hundred and eighty swab samples were taken from different sites in the slaughterhouses’ halls (surfaces, workers’ hands, boots, water, and chillers) followed by 240 swab samples collected from broiler carcasses after 4 process steps (defeathering, evisceration, washing and chilling). After bacterial culturing, isolation, and identification, all collected Data were subjected to descriptive and analytical statistic such as Chi square and ANOVA using SPSS. Low level of good practices in slaughterhouses was shown regarding programmed documented personal hygiene staff training and qualification for responsibilities 50.0% (n=6), preventative maintenance 41.7% (n=5), and appropriate use of personal facilities 58.3% (n=7). In addition to that, 66.7% (n=8) of examined premises showed difficulty in cleaning, inadequate ventilation and 33.3% (n=4) showed lack of sanitary facilities (dispensers, personnel changing rooms, toilets, washing basins). Unhealthy practices and habits were observed in majority of workers during work such as not washing hands before entering production areas, smoking, and eating and drinking in processing areas, beside low level of medical check in two thirds of them 66.7% (n=8). Bacterial growth was shown in 60.0% (n=108) and 58.80% (n=141) of slaughterhouses’ halls samples and meat samples respectively. There was significant difference between state of growth of slaughterhouses’ halls sample and farm location (χ2=7.22 and P-value = 0. 027) while a high significant difference in association between state of growth of meat samples and slaughterhouses’ location (χ2=43.02 and P-value=0. 000) was revealed. The Gram positive and Gram negative isolates were mostly detected in workers’ hands 19.60% (n=56) and 4.36% (n=12) respectively. The difference between growth state of bacteria in slaughterhouses’ halls samples and sample sites was highly significant (χ2=30.92 and P-value=0.000) and the highest growth was shown in workers’ hands. On the other hand, most of Gram positive and Gram negative bacteria in processing steps were detected after defeathering 14.40% (n=41) and evisceration 2.49% (n=7) steps. However, there was no significant difference resulting from association of state of bacterial growth of poultry meat samples and different process steps (χ2=5.14 and P-value=0.162). Slaughterhouses’ halls samples and meat samples were found contaminated with harmful pathogenic bacteria such as Salmonella spp. 1.41% (n=4), Escherichia coli 9.51% )n=27(, Staphylococcus aureus 7.75% (n=22), Citrobacter frundi 1.41% (n=4), and Micrococcus kristanae 8.75% (n=24). In conclusion, effective adoption of HACCP PRPs and biosecurity measures in Khartoum State has not been given serious attention beside lack of knowledge, resources, and absence of official authority’s role. Absence of proper “Farm to Table” hygiene policy in broiler production negatively affects poultry meat hygiene and consequently threatens public health. Therefore, formulation of suitable procedures and regulations by official authorities for implementing HACCP PRPs and biosecurity measures are needed to ensure poultry meat hygiene from primary stages of production till the end product.

Prevalence and Antibiograms of Salmonella in Commercially Produced Crocodile meat in Zimbabwe

2021 ◽  
Vol 36 (1) ◽  
pp. 1-14
Author(s):  
A.Z. Nhidza ◽  
C. Gufe ◽  
J. Marumure ◽  
Z. Makuvara ◽  
T. Chisango ◽  
...  
Keyword(s):  
Hazard Analysis ◽  
Control Point ◽  
Penicillin G ◽  
Diffusion Method ◽  
Biochemical Tests ◽  
Disk Diffusion Method ◽  
Critical Control Point ◽  
Meat Samples ◽  
Low Prevalence ◽  
Susceptibility Profiles

The presence of Salmonella in food products and emergence of antibiotic resistance are the major challenges facing public health policies. A total of 2749 crocodile meat samples obtained from the Central Veterinary Laboratories in Zimbabwe were screened for Salmonella specieswere collected from three Zimbabwean commercial farms between the year 2012 and 2019 for a retrospective observational study to determine the prevalence and magnitude of antibiotics resistant Salmonella species in crocodile meat. The isolation of Salmonella was in accordance with the ISO 6579:2002 and the antibiotic susceptibility testing was carried out based on Clinical and Laboratory Standard Institute’s recommendations by means of the Kirby-Bauer disk diffusion method. SILAB Database was used to determine the prevalence of Salmonella species. Prevalence was stratified by year and farms. Twenty Salmonella isolates were identified using biochemical tests, and 15 were confirmed by polymerase chain reaction (PCR). Antimicrobial susceptibility profiles of the confirmed Salmonella isolates were examined using 14 antibiotics. The overall prevalence of Salmonella species in crocodile meat samples was 0.5%. The prevalence of Salmonella species ranged from 0.04% to 0.44% in the crocodile meat samples and annual prevalence ranged from 0.01% to 1%. The highest prevalence of Salmonella (4.4%) was recorded in the year 2012. Salmonella isolates from one of the three tested farms were resistant to Erythromycin (73.33%), Ampicillin (80%), and Penicillin G (100%). Generally, Salmonella isolates displayed lower resistance to Cefepime, Ceftriaxone, Amikacin, Tetracycline, Ertapenem, Florfenicol, and Erythromycin (0-53.33%) whereas all Salmonella isolates showed susceptibility to Cefepime, Ceftriaxone, Ertapenem, and Florfenicol. Although the study indicates low prevalence of Salmonella species in crocodile meat, there is a need for strict implementation of Hazard Analysis Critical Control Point (HACCP) to reduce contamination rates in meat and its products

Microbial Contamination of Carcasses, Meat, and Equipment from an Iberian Pork Cutting Plant

2004 ◽  
Vol 67 (8) ◽  
pp. 1624-1629 ◽  
Author(s):  
TERESA RIVAS PALÁ ◽  
ANA SEVILLA
Keyword(s):  
Microbial Contamination ◽  
Hazard Analysis ◽  
Control Point ◽  
E Coli ◽  
Critical Control Point ◽  
Plate Counts ◽  
Intensive Rearing ◽  
Meat Samples ◽  
Type 3 ◽  
Meat Type

An assessment and follow-up of the microbial contamination of an Iberian pork cutting room is presented. Samples were taken from carcasses (n = 76), meat pieces (three types, n = 71), meat for dry-cured sausages (3 types, n = 66), and surfaces of equipment (n = 158). Aerobic plate counts (APC) at 37°C on meat pieces (primal cuts) were lower than on carcasses (3.62 log CFU/10 cm2 against 4.63 log CFU/10 cm2), probably owing to the removal of the skin. However, more than 80% of the meat pieces showed presence of Escherichia coli. For the three types of meat intended for dry-cured sausages, higher counts (P < 0.001) were found for meat type 3—an important cut obtained from the vertebral column—at 2.62 log CFU/g for E. coli; the particular surface used in the handling of meat type 3 also showed high counts (P <0.001) for E. coli. Consequently, attention should be paid to the hazard analysis critical control point plan at this stage. Salmonella was isolated from 3.94% of the carcass surfaces (perianal zone), 4.46% of meat pieces, and 13.58% of meat for dry-cured sausages. Moreover, the percentages for isolation of Salmonella from carcasses of Iberian pigs (extensive rearing) in our study were lower than those generally reported in the literature for “white pigs” (intensive rearing). Coagulase-positive Staphylococcus aureus was isolated in 31.82% of meat samples for dry-cured sausages, in 16.90% of meat pieces, and in 15.50% of the equipment after 4 h of work. Of the coagulase-positive strains isolated, 47.61% were producers of enterotoxin.

scholarly journals Prevalence of Campylobacter spp., Escherichia coli, and Salmonella Serovars in Retail Chicken, Turkey, Pork, and Beef from the Greater Washington, D.C., Area

2001 ◽  
Vol 67 (12) ◽  
pp. 5431-5436 ◽  
Author(s):  
Cuiwei Zhao ◽  
Beilei Ge ◽  
Juan De Villena ◽  
Robert Sudler ◽  
Emily Yeh ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Hazard Analysis ◽  
Control Point ◽  
Campylobacter Coli ◽  
Safety Education ◽  
E Coli ◽  
Critical Control Point ◽  
Food Borne ◽  
Meat Samples ◽  
Raw Meats

ABSTRACT A total of 825 samples of retail raw meats (chicken, turkey, pork, and beef) were examined for the presence of Escherichia coli and Salmonella serovars, and 719 of these samples were also tested for Campylobacter spp. The samples were randomly obtained from 59 stores of four supermarket chains during 107 sampling visits in the Greater Washington, D.C., area from June 1999 to July 2000. The majority (70.7%) of chicken samples (n = 184) were contaminated withCampylobacter, and a large percentage of the stores visited (91%) had Campylobacter-contaminated chickens. Approximately 14% of the 172 turkey samples yieldedCampylobacter, whereas fewer pork (1.7%) and beef (0.5%) samples were positive for this pathogen. A total of 722Campylobacter isolates were obtained from 159 meat samples; 53.6% of these isolates were Campylobacter jejuni, 41.3% were Campylobacter coli, and 5.1% were other species. Of the 212 chicken samples, 82 (38.7%) yielded E. coli, while 19.0% of the beef samples, 16.3% of the pork samples, and 11.9% of the turkey samples were positive for E. coli. However, only 25 (3.0%) of the retail meat samples tested were positive for Salmonella. Significant differences in the bacterial contamination rates were observed for the four supermarket chains. This study revealed that retail raw meats are often contaminated with food-borne pathogens; however, there are marked differences in the prevalence of such pathogens in different meats. Raw retail meats are potential vehicles for transmitting food-borne diseases, and our findings stress the need for increased implementation of hazard analysis of critical control point (HACCP) and consumer food safety education efforts.

Revisión: Los residuos en la inspección de la carne Review: Chemical residues in meat inspection

1997 ◽  
Vol 3 (6) ◽  
pp. 391-403 ◽  
Author(s):  
T. García ◽  
P.E. Hernández ◽  
B. Sanz ◽  
R. Martin
Keyword(s):  
Hazard Analysis ◽  
Control Point ◽  
Meat Inspection ◽  
Meat Production ◽  
Major Health ◽  
Critical Control Point ◽  
Safety Risks ◽  
Chemical Residues ◽  
Edible Tissues ◽  
Traditional Approaches

Edible tissues of food animals may contain residues of chemical substances harmful to human health, including animal drugs, mycotoxins and environmental contaminants. Current meat inspec tion activities rely mainly on visual inspection of the carcass and its offals at the slaughterhouse, and fail to detect chemical residues and enteric pathogens that are considered as major health risks associated with meat consumption. In contrast to traditional approaches, application of the hazard analysis critical control point (HACCP) to meat production and inspection, constitutes the best alternative to reduce the incidence of residues and to improve meat safety. Application of HACCP to meat inspection virtually eliminates the need for finished product testing by identi fying the safety risks inherent in the meat production steps, from farm to table, and devising preventive measures which can be monitored in order to control the process. Accordingly, there is also a need for the development and application of analytical methods of enough sensitivity that allow real-time monitoring of chemical residues in live animals and their tissues.

Current and emerging approaches to assuring the hygienic condition of red meats

1995 ◽  
Vol 75 (1) ◽  
pp. 1-13 ◽  
Author(s):  
C. O. Gill
Keyword(s):  
Hazard Analysis ◽  
Control Point ◽  
Subjective Assessment ◽  
Meat Inspection ◽  
Meat Production ◽  
Human Consumption ◽  
Hygienic Condition ◽  
Critical Control Point ◽  
Analysis Process ◽  
Red Meats

Current meat inspection activities exclude overtly diseased animals and carcasses from human consumption, and prevent grossly unhygienic meat production practices. However, they have signally failed to reduce the incidence of enteric pathogens on meat although the transmission of such organisms from asymptomatic animals is recognized as the major health risk associated with meat. The general hygienic condition of meat will be improved only if effective Hazard Analysis: Critical Control Point (HACCP) systems can be developed for meat production, preparation and distribution processes. The development of effective HACCP systems is impeded by the uncertain commitment of managements to product improvement, the lack of defined procedures for the objective identification of hazardous practices, and the persistence of the subjective assessment of the hygienic condition of product. The development of objective procedures for hazard analysis could precipitate the resolution of the other impediments to HACCP implementation. Key words: Meat hygiene, meat inspection, hazard analysis, process control, HACCP

scholarly journals Microbial Contamination Associated with “Wagashi-Cheese” Production in Sissala East District- Ghana

2016 ◽  
Vol 5 (4) ◽  
pp. 11
Author(s):  
Grace Annagmeng Mwini ◽  
Sarah Darkwa
Keyword(s):  
Microbial Contamination ◽  
Hazard Analysis ◽  
Control Point ◽  
Cow Milk ◽  
Critical Control Point ◽  
Critical Control Points ◽  
Standard Procedures ◽  
Significant Difference ◽  
Microbial Analysis

<p>The purpose of the study was to identify the possible sources of microbial contamination in the production of W<em>agashi-Cheese</em> in the Sissala East District, Upper West Region of Ghana. Also, the hygienic practices involved were examined. A survey, observation and experiment were used to collect data. Eighty (40 raw cow milk producers and 40 W<em>agashi-Cheese</em> producers) were randomly selected for the survey. Six (3 milk producers and 3 W<em>agashi –Cheese </em>producers) were purposively sampled for the experimental part. Microbial analysis of the milk and Wagashi-Cheese were carried out using the ISO and NMKL Analytical Standard procedures. Findings from the study revealed that milk and W<em>agashi-Cheese </em>producers do not practice optimal personal, food and environmental hygiene. Coliform, fecal coliform, <em>Escherichia coli</em>, Total mesophilic (PCA), yeast and mould were identified in both the milk and W<em>agashi-Cheese</em>. Dirty cow teat, unclean containers for receiving milk and improper handling of milk while transporting to Wagashi-Cheese centers were identified as Critical Control Points along the production line of W<em>agash-Cheese</em>. Post interventions showed significant (p ≤ 0.05) reduction in microbial levels in the samples analyzed. In conclusion, the application of Hazard Analysis Critical Control Point (HACCP) improved the quality of the final product. HACCP education and training for milk and W<em>agashi-Cheese </em>producers is highly recommended. In conclusion, there is a significant difference (P ≥ 0.05) in the microbial load of wagashi from the selected producers and thus the alternate hypothesis that there is no significant difference in the microbial loads of wagashi from different producers.</p>

Salmonella and food safety – why it matters for the British pig industry

2004 ◽  
Vol 31 ◽  
pp. 121-134
Author(s):  
Derek Armstrong
Keyword(s):  
Food Safety ◽  
Hazard Analysis ◽  
Control Point ◽  
Food Service ◽  
Meat Production ◽  
Foodborne Disease ◽  
Safe Handling ◽  
Critical Control Point ◽  
Service Companies ◽  
Slaughter Pigs

SummaryThe production and marketing of safe, wholesome pork, bacon, ham and other pork products is of paramount importance to British farmers, processors, retailers, food service companies and those that supply them. It is not possible to guarantee the complete absence of bacteria in meat production. Most bacteria found on meat are harmless, but some can cause foodborne disease. Control processes to reduce or eliminate food safety risks are applied to every stage of the production and marketing chain from the farm to the consumer.Key approaches to reducing the risk of foodborne disease include: reduction of zoonotic pathogens in slaughter pigs; prevention of cross contamination during activities such as transport and slaughter; safe processing of foods underpinned by HACCP (Hazard Analysis Critical Control Point); safe handling and preparation of food in the retail and catering sectors, including the adoption of HACCP principles and effective enforcement; safe handling and preparation of food in the home and enhanced surveillance of foodborne disease to monitor effectiveness of controls.

scholarly journals Antibiotic susceptibility pattern of extended spectrum β-lactamase ESBL producing gram negative bacilli in a tertiary care teaching hospital, Bareilly, India

2019 ◽  
Vol 8 (2) ◽  
pp. 228
Author(s):  
Anju Saxena ◽  
Sujata Singh ◽  
Rahul Goyal ◽  
Sumit Saxena
Keyword(s):  
Bacterial Growth ◽  
Tertiary Care ◽  
Health Concern ◽  
Resistant Bacteria ◽  
P Value ◽  
Sensitivity Testing ◽  
Gram Negative ◽  
Extended Spectrum ◽  
Significant Difference ◽  
Esbl Producers

Background: The increasing prevalence of infections caused by antibiotic-resistant bacteria makes empirical treatment of these infections difficult. Resistance to a wide variety of common antimicrobials has made the proliferation of extended spectrum β-lactamase (ESBL) producing strains a serious global health concern that has complicated treatment strategies and is very alarming. This study was undertaken to identify ESBL production in various gram negative bacilli isolated and to further study the antibiogram of ESBL producers and their contribution towards anti-microbial resistance.Methods: A total of 2008 samples were taken and studied for positive bacterial growth. Presence of ESBL positivity was detected using Kirby-Bauer sensitivity testing method and their antibiogram was studied. Data was analysed using IBM SPSS version 20. Chi-square test was applied wherever applicable to check the significant difference among the different groups. p value of ≤0.05 was considered to be significant.Results: A total of 2008 samples were studied. Out of which 655 gave positive bacterial growth and amongst these 312 were ESBL producers. Resistance to multiple classes of antibiotics was observed among ESBL producers and mostly imipenem, colistin and polymyxin B were the antibiotics which were sensitive to most of the strains.Conclusions: The frequency of ESBL producing strains among clinical isolates has been steadily increasing. Advance drug resistance surveillance and development of newer antibiotics is necessary to guide the appropriate and judicious antibiotic use.

Survey of Shell Egg Processing Plant Sanitation Programs: Effects on Non–Egg-Contact Surfaces

2004 ◽  
Vol 67 (12) ◽  
pp. 2801-2804 ◽  
Author(s):  
M. T. MUSGROVE ◽  
D. R. JONES ◽  
J. K. NORTHCUTT ◽  
P. A. CURTIS ◽  
K. E. ANDERSON ◽  
...  
Keyword(s):  
Hazard Analysis ◽  
Control Point ◽  
Processing Plant ◽  
Cross Contamination ◽  
Critical Control Point ◽  
Significant Difference ◽  
Processing Plants ◽  
Plate Counts ◽  
Contact Surfaces ◽  
Contamination Levels

To successfully implement a hazard analysis critical control point plan, prerequisite programs are essential. Sanitation standard operating procedures are an important part of such a plan and can reduce contamination levels so that food safety and quality are not adversely affected. Noncontact surfaces in the shell egg processing plants can serve as a reservoir of cross-contamination. The objective of this study was to assess the efficacy of sanitation programs used in a variety of shell egg processing facilities (in-line, off-line, and mixed operations). Fourteen different noncontact surfaces were sampled in nine commercial facilities across the southeastern United States. Non–egg-contact surfaces were defined as those where the shell egg does not come into direct contact with the surface or with the fluid from that surface. Gauze pads soaked in sterile phosphate-buffered saline were used for sampling at the end of a processing day (POST) and again the next morning prior to operations (PRE). Aerobic plate counts (APCs) and numbers of Enterobacteriaceae were determined. No significant differences (P &gt; 0.05) were found between POST and PRE counts for either population recovered from the 14 sampling sites. Only samples from the floor under the farm belts, nest-run loader, washers, and packer heads were reduced by 1 log CFU/ml of rinsate for APCs or Enterobacteriaceae counts. APCs of more than 104 CFU/ml of rinsate were recovered from many samples. Highest APCs were found on the floor under the farm belt and on shelves of the nest-run carts. High APCs were found on the wheel surface for off-line carts and on the loading dock floor. Highest Enterobacteriaceae counts were found in samples from the floor, drain, and nest-run egg cart shelves. A lack of significant difference between POST and PRE counts indicates that current sanitation programs could be improved. These data suggest that traffic patterns for the movement of eggs and materials through the plant should be reevaluated so that cross-contamination is reduced.

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