scholarly journals Cryopreservation of Semen of Mithun and Siri Bulls

2014 ◽  
Vol 12 (2) ◽  
pp. 147-153 ◽  
Author(s):  
P Dorji ◽  
V Pattarajinda ◽  
T Vongprolub
Keyword(s):  
Plasma Membrane ◽  
Sperm Concentration ◽  
Membrane Integrity ◽  
Plasma Membrane Integrity ◽  
Soya Lecithin ◽  
Semen Volume ◽  
Significant Difference ◽  
Semen Characteristics ◽  
The Mean ◽  
Mass Activity

The objective of the study was to assess the semen characteristics of Jersey, Mithun and Siri breeds of bulls and to compare the suitability of Tris Eggyolk Citrate and commercial soya lecithin based BIOXcell extenders for cryopreservation of semen from the these breeds of bulls. The mean semen volume was 4.55± 1.57, 4.86±1.33 and 4.83±1.72 ml in Jersey, Mithun and Siri bulls, respectively. Mean mass activity of 3, mean initial motility of 78.0±4.22, 80±4.47 and 78.33±4.08%, mean sperm concentration of 1416±484.19, 1530.91±517.41, 1122.83±293.68 x106 and mean livability of 69.20±6.81, 70.45±8.42, 76.67±8.17% was found in Jersey, Mithun and Siri bulls, respectively. There is no significant difference in all above mentioned variables among the breeds. The post-thaw motility of semen cryopreserved in Tris Eggyolk Citrate extender was found to be 45.50 ± 4.38, 46.82 ± 6.81 and 47.50 ± 8.22% in Jersey, Mithun and Siri bulls, respectively. Whereas in BIOXcell extender, it was 45.50 ± 5.99, 46.36 ± 5.05 and 49.17 ± 5.85 % in Jersey, Mithun and Siri bulls, respectively. Similarly, the plasma membrane integrity of thawed spermatozoa in Tris eggyolk extender was 17.50±2.32, 21.00±3.12 and 21.58±3.83% in Jersey, Mithun and Siri bulls, respectively. Whereas in BIOXcell extender, it was found to be 20.45±2.86, 20.86±2.281 and 22.33±3.24% in Jersey, Mithun and Siri bulls, respectively. No significant difference (p>0.05) was observed in post-thaw motility as well as in plasma membrane integrity between extenders and between breeds, and there was no significant effect (p>0.05) of breed x extender interaction. It can be concluded that the semen characteristics of Siri and Mithun bulls fall with the range established for other breeds of bulls. Furthermore, both Tris eggyolk and BIOXcell extenders are equally suitable for the cryopreservation of semen from these three breeds of Bulls.DOI: http://dx.doi.org/10.3329/bjvm.v12i2.21277 Bangl. J. Vet. Med. (2014). 12 (2): 143-153 

The effects of chronic administration of ghrelin on rat sperm quality and membrane integrity

2009 ◽  
Vol 59 (2) ◽  
pp. 159-168 ◽  
Author(s):  
Arash Kheradmand ◽  
Majid Taati ◽  
Homayoon Babaei
Keyword(s):  
Plasma Membrane ◽  
Treatment Group ◽  
Sperm Motility ◽  
Sperm Quality ◽  
Sperm Concentration ◽  
Membrane Integrity ◽  
Chronic Administration ◽  
Control Group ◽  
Plasma Membrane Integrity ◽  
Significant Difference

AbstractAlthough ghrelin acts as a modulator of feeding behavior and energy metabolism in the central nervous system, recent studies have implicated the peripheral actions of ghrelin in reproductive tissues. Here, we investigated the effects of chronic administration of ghrelin on the motility, plasma membrane integrity and concentration of rat spermatozoa. 45-d male Wistar rats were scheduled for the study and were divided into control and treatment groups. In the treatment group, 1 nmol of ghrelin was administered as sc injection for 10 consecutive days or vehicle (physiological saline) to the control rats. Sperm collection was achieved by killing of the rats on days 15, 25 and 50 after first injection. Total sperm motility and forward progressive movement did not exhibit significant difference during the experiment, although, there was a tendency for greater motion rate on d 15 and 25 in the treated rats compared to the control group. Plasma membrane integrity (HOS-reacted spermatozoa) was significantly higher in the treated animals, especially on day 15 as well as day 25, because of possible antioxidant properties of ghrelin. This value was statistically higher on day 15 than that of day 25 (P <0.05). Likewise, there was a significant correlation between the FPM (P <0.0001, r = 0.79) and TSM (P <0.01, r = 0.52) with the HOS test percentage in the treatment group. It was not observed statistically difference in the sperm concentration between groups during all of the experimental days. In conclusion, chronic administration of ghrelin (similar to induced by energy deficiency such as fasting) increased the integrity of sperm membrane, however, the sperm motility and concentration did not display any alterations.

scholarly journals Correlation Between Ultrasonic Testicular Volume and Seminal Fluid Analysis in Men with Infertility

2021 ◽  
Vol 3 (1) ◽  
pp. 103-107
Author(s):  
Sadiq Abu ◽  
Hadijat O. Kolade-Yunusa ◽  
Terkaa Atim ◽  
F. Ehimatie Obakeye ◽  
Nuhu K. Dakum
Keyword(s):  
Sperm Motility ◽  
Teaching Hospital ◽  
Seminal Fluid ◽  
Sperm Concentration ◽  
Testicular Volume ◽  
Seminiferous Tubules ◽  
Testicular Function ◽  
Semen Volume ◽  
Significant Difference ◽  
The Mean

Background: Infertility is a major cause of marital disharmony in Nigeria because of the high premium placed on childbearing. Unfortunately, the blame is on the woman most times in Nigeria. Seminiferous tubules comprise 80-90% of testicular mass. Thus, the testicular volume is an index of spermatogenesis. Therefore, accurate testicular volume will help in assessing testicular function if there is no obstruction. This study was conducted to evaluate the correlation between testicular volume, measured by ultrasound and conventional sperm parameters (semen volume, sperm concentration, sperm motility and sperm morphology) in men with infertility presenting to Urology Division, University of Abuja Teaching Hospital, Gwagwalada Abuja. Methodology: This was a descriptive cross sectional study, male subjects with infertility presenting to University of Abuja Teaching Hospital were recruited into the study. The testicular volume of all the subjects was measured by ultrasonography. The semen samples were collected by the process of masturbation on day five of sexual abstinence and analyzed according to WHO criteria 2010. Collated data were analyzed using SPSS version 20.0. P-value<0.05 was taken as statistically significant. Results: The average total testicular volume (TTV) of men with infertility was 19.83 ml while mean testicular volume (MTV) was 10.24 ml. There was a statistically significant difference between MTV and semen volume (r=0.391, p=0.000), and sperm concentration (r=0.639, p=0.000). There was a weak and insignificant correlation between MTV and sperm motility and MTV and morphology (r=0.216, p=0.055 and r=0.076, p=0.502) respectively. Linear regression analysis showed significant impact of MTV (P<0.001) on sperm concentration with a critical MTV of ≤10ml.0 indicating poor testicular function (severe oligospermia) Conclusion: The mean ultrasonic testicular volume in men with infertility in this study was 10.24ml and there was a significant positive correlation of MTV with semen volume and sperm concentration. Although, there was correlation of MTV with sperm motility and morphology, this was not significant. The critical mean ultrasonic testicular volume indicating poor testicular function (severe oligospermia) was found to be 10ml. This study has shown that the mean ultrasonic testicular volume can be a pointer to semen volume and sperm concentration in evaluating men with infertility.

scholarly journals EFFECT OF ADDING PENTOXIFYLLINE AND NITRIC OXIDE TO TRIS EXTENDER ON SOME POST-CRYOPRESERVED SEMEN ATTRIBUTES OF HOLSTEIN BULLS

2020 ◽  
Vol 51 (2) ◽  
pp. 619-628
Author(s):  
Mohammed & et al.
Keyword(s):  
Nitric Oxide ◽  
Plasma Membrane ◽  
Membrane Integrity ◽  
Control Group ◽  
Plasma Membrane Integrity ◽  
Abnormal Sperm ◽  
Semen Characteristics ◽  
Holstein Bulls ◽  
Time Periods ◽  
Live Sperm

This study was aimed to explore the effect of adding pentoxifylline (PTX) and nitric oxide (NO) to Tris extender on some post-cryopreserved semen attributes of Holstein bulls for different preservation periods (cooling at 5◦C, 48 hrs., 1, 2 and 3 months post cryopreservation, PC). Seven Holstein bulls of 2.5-3 years old were used in the current study during the period from 20th November, 2017 to 20th August, 2018. Pooled semen was equally divided into three groups within one experiment. PTX (0.192 g / 100 ml extender) and NO (0.018 g / 100 ml extender) were added to Tris extender and comparisons in response were made with the control group (Tris extender, C). The PTX group exhibited greater (P≤ 0.01) sperm's cell individual motility percentage as compared with the C group at cooling (5ºc) and 48 hr PC periods, while, PTX and NO groups were superior in these percentages at the remaining PC periods than C group. Excluding data of 2 months PC, greater (P≤ 0.01) live sperm percentage was observed in PTX and NO groups in comparison with the C group at all preservation periods. Lesser (P≤ 0.01) abnormal sperm percentage were noticed for PTX and NO groups as compared with the C group at all preservation time periods. The PTX and NO groups exhibited greater   (P≤ 0.01) acrosome and plasma membrane integrity percentages in comparison with the C group at all preservation time periods. In conclusion, adding PTX and NO to Tris extender enhanced some of  PC semen characteristics of Holstein bulls at different preservation periods.

scholarly journals The effect of carob (Ceratonia siliqua) bean extract on male New Zealand White rabbit semen

2018 ◽  
Vol 26 (3) ◽  
pp. 209 ◽  
Author(s):  
A. Ata ◽  
O. Yildiz-Gulay ◽  
S. Güngör ◽  
A. Balic ◽  
M.S. Gulay
Keyword(s):  
Plasma Membrane ◽  
New Zealand ◽  
Plasma Concentration ◽  
Seminal Plasma ◽  
Sperm Concentration ◽  
Membrane Integrity ◽  
Ceratonia Siliqua ◽  
Plasma Membrane Integrity ◽  
Protein Levels ◽  
Sperm Plasma Membrane

<p>The carob tree (Ceratonia siliqua) grows naturally in the Mediterranean region. The empiric use of carob cures for their aphrodisiac properties is very common in Turkey. Thus, the experiment was conducted to determine the effects of carob bean extracts on some reproductive parameters in male New Zealand White rabbits. During the adaptation period (stage 1), 6-8 mo old rabbits were trained in semen collection for 30 d. At the beginning of the treatment period (stage 2), rabbits were assigned randomly to 2 groups of 8 animals each. For a period of 49 d (1 spermatogenesis duration), one group was treated with a daily oral dose (10 mL) of carob extract and the other group received the corresponding volume of tap water. Semen was collected weekly. Semen samples taken at week 1 and 7 were analysed separately. At the beginning of stage 2, no differences were observed in the volume and pH of the ejaculate, sperm concentration, percentage of motility, percentage of live spermatozoa, percentage of sperm plasma membrane integrity, plasma concentration of testosterone, and seminal plasma protein levels between the control and carob extract treated animals. Similarly, at the end of stage 2, there were no differences in the volume and pH of the ejaculate, motility percentage, the percentage of live spermatozoa, percentage of sperm plasma membrane integrity, and the seminal plasma protein levels between the control and the carob extract treated animals. However, sperm concentration (P&lt;0.05), plasma concentration of testosterone (P&lt;0.05), and percentage of change in spermatozoa concentration (P&lt;0.02) between groups were affected at the end of stage 2. The data suggested that the use of carob cures prepared by boiling carob fruit could have beneficial influences on sperm concentration in rabbits.</p>

scholarly journals Testosterone and semen seasonality for the sand tiger shark Carcharias taurus†

2019 ◽  
Vol 102 (4) ◽  
pp. 876-887 ◽  
Author(s):  
Jennifer T Wyffels ◽  
Robert George ◽  
Lance Adams ◽  
Cayman Adams ◽  
Tonya Clauss ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Semen Quality ◽  
Sperm Concentration ◽  
Membrane Integrity ◽  
Tiger Shark ◽  
Motile Sperm ◽  
Testosterone Concentration ◽  
Plasma Membrane Integrity ◽  
Carcharias Taurus

Abstract Understanding the fundamental reproductive biology of a species is the first step toward identifying parameters that are critical for reproduction and for the development of assisted reproductive techniques. Ejaculates were collected from aquarium (n = 24) and in situ (n = 34) sand tiger sharks Carcharias taurus. Volume, pH, osmolarity, sperm concentration, motility, status, morphology, and plasma membrane integrity were assessed for each ejaculate. Semen with the highest proportion of motile sperm was collected between April and June for both in situ and aquarium sand tiger sharks indicating a seasonal reproductive cycle. Overall, 17 of 30 semen samples collected from aquarium sharks from April through June contained motile sperm compared to 29 of 29 of in situ sharks, demonstrating semen quality differences between aquarium and in situ sharks. Sperm motility, status, morphology, and plasma membrane integrity were significantly higher (P &lt; 0.05) for in situ compared to aquarium sand tiger sharks. Testosterone was measured by an enzyme immunoassay validated for the species. Testosterone concentration was seasonal for both aquarium and in situ sharks with highest concentrations measured in spring and lowest in summer. In situ sharks had higher (P &lt; 0.05) testosterone concentration in spring than aquarium sharks. This study demonstrated annual reproduction with spring seasonality for male sand tiger sharks through marked seasonal differences in testosterone and semen production. Lower testosterone and poorer semen quality was observed in aquarium sharks likely contributing to the species’ limited reproductive success in aquariums.

scholarly journals Comparative Study of Gir Cattle and Surti Buffalo Bulls Semen under Middle Gujarat Climate

2017 ◽  
Vol 13 (01) ◽  
Author(s):  
P. J. Chaudhary ◽  
A. J. Dhami ◽  
D. V. Chaudhari ◽  
K. K. Hadiya ◽  
J. A. Patel
Keyword(s):  
Sperm Concentration ◽  
Membrane Integrity ◽  
Mean Values ◽  
Abnormal Sperm ◽  
Buffalo Semen ◽  
The Mean ◽  
Mass Activity ◽  
Live Sperm ◽  
Surti Buffalo ◽  
Fresh Semen

This study was undertaken during the favourable breeding season of the year 2016-17 on healthy mature Gir cattle and Surti buffalo bulls, three each, at Sperm Station of the College. The ejaculates (9/bull, total 54) collected in the morning using artificial vagina were evaluated for routine seminal attributes, including acrosomal and plasma membrane integrity. The mean values of ejaculate volume, sperm concentration, mass activity (0-5 score), individual sperm motility, live sperm, abnormal sperm, intact acrosome and HOST reactive sperms observed in fresh semen of Gir cattle and Surti buffalo bulls were 6.20±0.42 and 3.34±0.23 ml (P less than 0.01), 1169.44±61.71 and 846.30±54.82 million (P less than 0.01), 3.44±0.09 and 3.42±0.08, 76.53±0.53 and 80.76±0.39 % (P less than 0.05), 81.00±1.32 and 84.73±0.78 % (P less than 0.05), 6.00±0.37 and 5.81±0.40 %, 95.59±0.35 and 95.54±0.25 % as well as 80.30±1.90 and 84.58±0.88 % (P less than 0.05), respectively. The variation between cattle and buffalo semen was significant for most of these traits. The variations between bulls within breed were however not significant. Significant correlations were observed only between mass activity and initial motility (0.62), and live and abnormal sperm (-0.41) in Gir bulls, and for ejaculate volume with sperm concentration (-0.56) and abnormal sperm (0.45), and between live and HOS reactive sperms (0.48) in Surti bulls.

scholarly journals Influence of seasonality on the physiological and seminal parameters of buffaloes in the western region of Pará1

2020 ◽  
Vol 40 (12) ◽  
pp. 1048-1053
Author(s):  
Sâmia R.S. Castro ◽  
Leticia S. Rebelo ◽  
Onildo S. Fernandes Junior ◽  
Alessandra S. Belo-Reis ◽  
Kedson A.L. Neves ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Animal Welfare ◽  
Rainy Season ◽  
Statistical Difference ◽  
Thermal Environment ◽  
Membrane Integrity ◽  
Morphological Characteristics ◽  
Plasma Membrane Integrity ◽  
Sperm Plasma Membrane ◽  
Mass Activity

ABSTRACT: Awareness of the physiological changes that occur when animals are subjected to climatic changes that are considered stressful is essential to maintain animal welfare and to be able to exploit their reproductive potential efficiently and rationally. The present study was carried out to evaluate climatic variables’ influence on physiological parameters, and Murrah buffalo ejaculates reared in a humid tropical climate in the Amazon. The immediate analyzes pertinent to the physical and morphological characteristics of the ejaculates were carried out and corresponded in the rainy season (RS) volume of 3.4±2.0mL; the mass activity of 4.4±0.5; motility of 80.4±5.6%; vigor of 4.4±0.4; concentration of 657,300±237,865.1 x 106sptz/mL; major defects of 9.0±2.6%; minor defects of 11.2±3.9%; total defects 20.2±5.3% and sperm plasma membrane integrity (SPMI) 84.8±5.6%, whereas in the non-rainy season (nRS), the results were 4.0±2.1mL; the mass activity of 3.0±1.0; motility of 56.2±13.4%; vigor of 3.0±1.0; concentration of 586,000±291,925.9 x 106sptz/mL; major defects of 20.8±9.9%; minor defects of 27.5±6.3%; total defects 48.3±9.3% and SPMI of 57.9±12.4%. Furthermore, a statistical difference (P<0.05) was observed for the parameters mass activity, motility, vigor, major defects, minor defects, total defects, and sperm plasma membrane integrity between both periods. The data on heart frequency, superficial temperature (head, back, groin, and scrotal pouch) showed a statistical difference between both periods (P<0.05). To conclude is necessary specific management in the non-rainy season that thermal stress is not a determining factor in reducing the reproductive quality of buffaloes; it is necessary to use means to improve animal welfare; one alternative is to use baths regularly for these animals or provide constant access to areas of rivers or lakes, as well as shading, preventing the buffaloes from being directly exposed to the unfavorable thermal environment.

scholarly journals Sheng Jing Decoction Can Promote Spermatogenesis and Increase Sperm Motility of the Oligozoospermia Mouse Model

2021 ◽  
Vol 2021 ◽  
pp. 1-9
Author(s):  
Guang Yan ◽  
Fang Tian ◽  
Peng Liu ◽  
Jianming Sun ◽  
Jianmin Mao ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Sperm Motility ◽  
Sperm Morphology ◽  
Sperm Concentration ◽  
Membrane Integrity ◽  
Testicular Tissue ◽  
Whole Body ◽  
Plasma Membrane Integrity ◽  
Normal Sperm ◽  
Sperm Plasma Membrane

Sheng Jing Decoction (SJD), as a traditional Chinese medicine prescription, is mainly be used to treat male infertility. However, the pharmacological functions and molecular mechanisms of SJD are poorly understood. In this study, we investigated the functions of SJD on spermatogenesis and sperm motility and explored the potential mechanisms involved. Here, we demonstrated that high, medium, and low doses of SJD are effective in restoring the impairments of the whole body and testicular tissue by cyclophosphamide inducing and to rescue the damage of testicular tissue cells including Sertoli cells and germ cells. SJD can partly restore the decrease in sperm concentration, sperm vitality, sperm motility, and normal sperm morphology rate in oligozoospermic mouse models. Ki67 staining analyses confirm SJD can promote testicular tissue cell proliferation. Real-time RT-PCR analyses also reveal that SJD can upregulate the expression of proliferation-associated gene Lin28a and differentiation-associated genes Kit, Sohlh2, and Stra8. SJD can also reduce the impairment of mitochondrial membrane potential (MMP) and sperm plasma membrane integrity by cyclophosphamide inducing. Our results reveal that SJD is effective in improving both sperm quantity and quality by increasing the sperm concentration, sperm vitality, sperm motility, and normal sperm morphology rate. SJD can promote spermatogenesis by upregulating the expression of the proliferation-associated gene Lin28a and the differentiation-associated genes (Kit, Sohlh2, and Stra8). SJD can sustain MMP and sperm plasma membrane integrity to increase sperm motility.

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