scholarly journals Evaluating the ability of semen cryopreservation of Blance Blue Belge bull cattle in Vietnam

2021 ◽  
Vol 19 (2) ◽  
pp. 237-244
Author(s):  
Nguyen Huu Duc ◽  
Pham Thu Giang ◽  
Tran Thi Binh Nguyen ◽  
Nguyen Thi Mai ◽  
Bui Dai Phong
Keyword(s):  
Sperm Motility ◽  
Sperm Concentration ◽  
Joint Stock Company ◽  
Stock Company ◽  
Cattle Breeding ◽  
Semen Cryopreservation ◽  
Semen Collection ◽  
Frozen Semen ◽  
Semen Volume ◽  
Fresh Semen

The objective of this study was to determine the semen cryopreservation capacity of BBB bulls in Hanoi-Vietnam. Research conducted on the fresh semen collected from 05 BBB bulls. Results showed that semen color was normal (milky white, ivory white, ivory yellow), semen volume ranged from 6.35 mL to 7.48 mL (P <0.05), initial motility of semen ranged from 80.53% to 82.92% (P <0.05), sperm concentration in semen  ranged from 1.02 x 109 sperms/ml to 1.12 x 109 sperms/mL (P <0.05), abnormal sperm ratio ranged from 6.45% to 8.12% (P <0.05), alive sperm ratio ranged from 76.34% to 82.97% (P <0.05), sperm motility after thawing from straw semen ranged from 71.33% to 75.92% (P<0.05). In conclusion, successfully semen collection from 05 breeding BBB bulls at Hanoi Cattle Breeding Joint Stock Company, semen samples had normal color and good quantity and quality, suitable for production of frozen semen; and semen cryopreservation of straws of the 05 bull BBB semen mentioned at -196oC, sperm motility after freezing-thawing reached the economic and technical norms of 675/2014 of the Ministry of Agriculture and Rural Development.

scholarly journals Establishment of semen collection technique using electroejaculator and semen cryopreservation of Javan leopard (Panthera pardus melas Cuvier, 1809)

2021 ◽  
pp. 3156-3163
Author(s):  
Bongot Huaso Mulia ◽  
Ardyta Widianti ◽  
Jansen Manansang ◽  
Dedi Rahmat Setiadi ◽  
Vincentia Trisna Yoelinda ◽  
...  
Keyword(s):  
Sperm Motility ◽  
Sperm Morphology ◽  
Total Concentration ◽  
Sperm Concentration ◽  
Panthera Pardus ◽  
Semen Collection ◽  
Normal Sperm ◽  
Frozen Semen ◽  
Semen Volume ◽  
Microscopic Evaluation

Background and Aim: The Javan leopard (Panthera pardus melas Cuvier, 1809) is a subspecies of Panthera pardus spp., spread across the African and Asian regions. Information on reproductive aspects is crucial for wild animals, including the Javan leopard. In this study, we aimed to develop electroejaculator (EE) techniques and evaluate cryopreservation success in Javan leopard semen. Materials and Methods: The semen of four adult Javan leopards was collected once a week using EE. Placement of the EE probe in the rectum was performed after ultrasound imaging (ultrasonography) to determine the prostate body location. The semen obtained was then evaluated macroscopically and microscopically. Three Javan leopards were used for cryopreservation. The ejaculate was divided into two parts [i.e., one part diluted with AndroMed® (Minitüb, Tiefenbach, Germany) and the other part with Steridyl® (Minitüb, Tiefenbach, Germany)] at a 1:1 ratio immediately after collection and evaluation. The semen was then packed in a 0.25 mL MiniStraw® (Minitüb, Tiefenbach, Germany) then equilibrated at 4°C for 2 h. After equilibration, the straw was then frozen in liquid nitrogen vapor. Frozen semen was then stored in containers until further evaluation. Results: The results showed that ejaculation response occurred at all levels of stimulation, while erections did not always occur. The fastest ejaculation and erection occurred at the fourth voltage. The macroscopic evaluation showed that the semen volume was 0.80±0.26 mL, cloudy white, pH 7.44±0.14, and with watery semen consistency. The microscopic evaluation showed that the sperm motility was 66.98±0.39%, with sperm viability of 75.6±1.79%. Sperm concentration was 62.17±46.95×106 mL–1 with a total concentration of 42.14±23.51×106 cells. Normal sperm morphology is only 40.72±6.26%. Conclusion: This study concluded that the development of a semen collection technique using an EE preceded by imaging of the EE probe location using ultrasound was effective for the ejaculation of Javan leopards. The characteristics of the semen of the Javan leopard showed moderate semen volume, sperm motility, and viability. Javan leopard showed low sperm concentration and normal sperm morphology.

scholarly journals Individual variation in fresh and frozen semen of Bali bulls (Bos sondaicus)

2020 ◽  
Vol 13 (5) ◽  
pp. 840-846
Author(s):  
R. Indriastuti ◽  
M. F. Ulum ◽  
R. I. Arifiantini ◽  
B. Purwantara
Keyword(s):  
Dna Fragmentation ◽  
Sperm Motility ◽  
Individual Variation ◽  
Sperm Concentration ◽  
Individual Factors ◽  
Abnormal Morphology ◽  
Frozen Semen ◽  
Semen Volume ◽  
Fresh Semen

Aim: This study aimed to analyze the individual factors influencing the sperm quality of Bali bulls at Baturiti Artificial Insemination (AI) center. Materials and Methods: Semen that was ejaculated from nine Bali bulls was collected using artificial vaginas (n=5/bull). Semen ejaculates were evaluated immediately after collection to measure the quality of the fresh semen, including semen volume, sperm concentration, progressive motility, membrane integrity (MI), and abnormal morphology. Frozen semen was evaluated for progressive sperm motility, concentration, viability, MI, abnormal morphology, and deoxyribonucleic acid (DNA) fragmentation. Other secondary data, focusing on semen quantity (semen volume and sperm concentration), were also collected from frozen the semen production data of the Baturiti AI center from 2017 to 2019. Data were analyzed statistically using a completely randomized design, and one-way analysis of variance was applied to find differences among individual bulls. Results: Significant differences (p<0.05) were found among the bulls in semen volume, sperm motility, concentration, and MI of the fresh semen. Significant differences (p<0.05) were also found among the bulls in sperm motility, viability, MI, abnormal morphology, and DNA fragmentation of the frozen semen. Conclusion: Individual variation in all the tested sperm parameters of the fresh semen of Bali bulls, except sperm viability and abnormalities, was noted. Similarly, individual variation in all the tested sperm parameters in frozen semen, except sperm concentration, was noted. Therefore, individual factors can be used for selecting a superior bull in Bali cattle.

Effect of different extenders on quality of frozen-thawed boar semen

2015 ◽  
Vol 49 (6) ◽  
Author(s):  
S Frydrychová ◽  
A Lustyková ◽  
E Václavková ◽  
J Lipenský ◽  
M Rozkot
Keyword(s):  
Sperm Motility ◽  
Aspartate Aminotransferase ◽  
Sperm Concentration ◽  
Holding Time ◽  
Holding Period ◽  
Semen Volume ◽  
Significant Difference ◽  
Boar Semen ◽  
Fresh Semen

The objective of this study was to investigate the effect of using different extenders <italic>viz.</italic> Androhep, Safecell Plus and SUS during cryopreservation on quality of frozen-thawed boar semen. Semen volume, sperm motility, sperm concentration, percentage of morphologically abnormal spermatozoa, total number of spermatozoa per ejaculate and activity of the enzyme aspartate aminotransferase (AST) were assessed in fresh semen collected from 39 fertile AI boars. Semen from each boar was divided into three portions and diluted 1:1.5 in extender Androhep, Safecell Plus and SUS and keep at 17°C for 15-h holding time before cryopreservation. Then sperm was cryopreserved. Straws were thawed in a water bath at 38°C for 40s and post-thaw sperm motility with AST activity was assessed. Significant difference in post-thaw sperm motility was found between extender Androhep and Safecell Plus (P<0.05). AST activity did not differ significantly between tested extenders (P>0.05). In conclusion, the results of the study indicate that using Safecell Plus extender during holding period before cryopreservation significantly affected post-thaw sperm motility.

scholarly journals Relationship between scrotal circumference and semen parameters in crossbred bulls

1970 ◽  
Vol 26 (2) ◽  
pp. 61-67 ◽  
Author(s):  
MA Latif ◽  
JU Ahmed ◽  
MMU Bhuiyan ◽  
M Shamsuddin
Keyword(s):  
Sperm Motility ◽  
Sperm Concentration ◽  
Semen Parameters ◽  
Sperm Production ◽  
Positive Correlation ◽  
Scrotal Circumference ◽  
Semen Volume ◽  
The Relationship ◽  
Fresh Semen

The relationship between the scrotal circumference and semen volume, sperm concentration and number of sperm per ejaculate of 12 crossbred bulls [4 Local × Friesian, 4 Local × Sahiwal] was studied. Semen of three consecutive collections at seven day intervals was evaluated. The age of bulls at first collection varied between 15 and 20 months. Soon after collection, volume, sperm concentration, number of spermatozoa per ejaculate and initial sperm motility of fresh semen were measured. Scrotal circumference was correlated with semen volume, sperm concentration and number of spermatozoa per ejaculate in both groups. A significant (P<0.04) positive correlation (r = 0.72) was observed between scrotal circumference and volume of semen, and between scrotal circumference and number of sperm production per ejaculate. Significant (P<0.05) variation was observed in different semen parameters between bulls within the same group, but no significant (P>0.05) variation was found between the two groups. It is suggested that crossbred bulls aged 18 months or over, with scrotal circumference more than 30 cm, yielded good quality semen. DOI: 10.3329/bvet.v26i2.4952 Bangl. vet. 2009. Vol. 26, No. 2, 61-67

scholarly journals Pengaruh Suplementasi Vitamin E, Mineral Selenium dan Zink Terhadap Konsumsi Nutrien, Produksi dan Kualitas Semen Sapi Simental

2014 ◽  
Vol 14 (1) ◽  
pp. 6-16
Author(s):  
Fitrah Khairi ◽  
Anis Muktiani ◽  
Yon Supri Ondho
Keyword(s):  
Vitamin E ◽  
Sperm Motility ◽  
Rainy Season ◽  
Dry Matter ◽  
Semen Quality ◽  
Sperm Concentration ◽  
Cement Production ◽  
Semen Volume ◽  
Nutrient Consumption ◽  
Fresh Semen

(Effect of supplementation of vitamin E, Selenium and Zinc mineral against to nutrient consumption, production and quality cement simental cow)ABSTRACT. Simental bulls often experience a decline in production and semen quality in the rainy season, which have a higher humidity and high rainfall. This study aims to maintain nutrient consumption, production and semen quality Simental bulls during the rainy season through the supplementation of Vitamin E, Selenium and Zinc Minerals. The study was conducted in July-December 2012 at the Artificial Insemination Center (BIB) Unggaran. The research material used in this study were 12 males Simental cows BIB Unggaran grouped by age. The experimental design used in this study is a randomized block design (RBD) with 3 treatments T1 (ration + Vit. E), T2 (ration + Vit. E + Se), T3 (ration + Vit. E + Se + Zn) and 4 groups as replicates. Parameters measured were dry matter intake and nutrient consumption, production and semen quality. Cement production was measured from fresh semen volume during the study, whereas semen quality measured motility and concentration of spermatozoa from fresh semen. Data consumption of dry matter and nutrient intake obtained were processed using analysis of variance, followed by Duncan test. While data on production and semen quality were analyzed descriptively. The results showed that the combination of vitamin E supplementation, minerals Selenium and Zinc did not affect dry matter intake, total digestible nutriens and crude protein, but resulted in a decrease in the percentage of shelter, semen volume, sperm motility and concentration smaller than before treatment. Treatment T1, T2 and T3 resulted in a decrease in the number of shelter respectively 41,55%, 19,56% and 13,63% compared to before treatment, whereas a decrease in semen volume unchanged at 44,9 %, 43,7% and 40,99%. Sperm motility due to treatment T1, T2 and T3 respectively decreased by 55,87%, 22,10% and 13,63% compared to before treatment. In line with sperm motility, sperm concentration in treatment T , T2 and T3, respectively decreased by 49,16%, 22,85% and 14,88%. The conclusion is a combination of vitamin E supplementation, minerals Selenium and Zinc can prevent the decline in cement production, sperm motility and sperm concentration Simental bulls during the rainy season.

scholarly journals Individual Variation in Semen Characteristics of Murrah Buffalo Bulls

2020 ◽  
Vol 9 (3S) ◽  
pp. 58-60
Keyword(s):  
Sperm Motility ◽  
Individual Variation ◽  
Artificial Insemination ◽  
Recovery Rate ◽  
Sperm Concentration ◽  
Murrah Buffalo ◽  
Frozen Semen ◽  
Semen Characteristics ◽  
The Individual ◽  
Fresh Semen

This study evaluated the variation in semen characteristics between two individual Murrah buffalo bulls. Two hundred and ninety semen samples were involved in this study. The results showed that most of the semen had a milky white color with moderate consistency. The Bull B1 tended to have higher proportion of creamy color and moderate consistency than the Bull B2. The ejaculatory volume is varied (P<0.01) between Bull B1 and Bull B2. However, semen pH and sperm concentration of Bull B1 and B2 were equal (P>0.05). The individual bull had a highly significant effect (P<0.01) on sperm motility of fresh semen. The individual variation (P<0.05) also found on sperm motility of before freezing. Whereas, sperm motility of post-thawing semen and recovery rate value were not significantly influenced (P>0.05). Moreover, it was found that the frozen semen production differed (P<0.01) between two individual bulls, with bull B1 had higher production than the bull B2. This study clearly demonstrates that the frozen semen production of Murrah buffalo is highly dependent on the individual bull. However, the variation in frozen semen production between two bulls studied here is still qualified to be used for artificial insemination.

scholarly journals Cryopreservation and quality assessment of boar semen collected from bulk samples

2019 ◽  
Vol 64 (No. 5) ◽  
pp. 209-216 ◽  
Author(s):  
R Ratchamak ◽  
T Vongpralub ◽  
W Boonkum ◽  
V Chankitisakul
Keyword(s):  
Sperm Motility ◽  
Semen Quality ◽  
Sperm Quality ◽  
Sperm Concentration ◽  
Bulk Sample ◽  
Energy Status ◽  
Semen Volume ◽  
Boar Semen ◽  
Live Sperm ◽  
Fresh Semen

The purpose of this study was to examine sperm quality after cryopreservation of ejaculates collected as a bulk sample, which is routinely part of semen collection, and to compare this quality with the sperm-rich fraction in boars. Ejaculates were collected as sperm-rich fractions (SRF) and bulk samples (BE) using a gloved-hand technique. Fresh semen quality in terms of semen volume, sperm concentration, total sperm motility and pH were conventionally evaluated. Then, semen was cryopreserved using the liquid nitrogen vapour method. The post-thaw sperm quality was evaluated by assessing sperm motility, live sperm with normal apical ridge and high mitochondrial energy status, lipid peroxidation was evaluated using CASA and fluorescent multiple staining and MDA levels were determined using a spectrophotometer, respectively. In terms of fresh semen quality, sperm motility in fresh semen did not differ significantly between the two groups. The treatment with the greater mean volume (BE; P &lt; 0.05) had a lower mean sperm concentration (P &lt; 0.05); meanwhile, the mean ejaculate pH collected as BE was more basic compared with SRF (P &lt; 0.05). However, there were no significant post-thaw quality changes between sperm-rich fractions and bulk samples of semen. In conclusion, ejaculates can be collected as bulk samples without the need to classify fractions for boar semen cryopreservation.

STUDY ON BODY WEIGHT AND SCROTAL CIRCUMFERENCE AND ITS CORRELATIONS WITH SEMEN PRODUCTION TRAITS IN CROSSBRED JERSEY BULLS

2016 ◽  
Vol 12 (1) ◽  
Author(s):  
A. Gopinathan ◽  
S.N. Sivaselvam ◽  
S.M.K. Karthickeyan ◽  
R. Venkataramanan ◽  
K. Kulasekar
Keyword(s):  
Body Weight ◽  
Sperm Motility ◽  
Tamil Nadu ◽  
Sperm Concentration ◽  
The Body ◽  
Production Traits ◽  
Scrotal Circumference ◽  
Frozen Semen ◽  
Semen Volume ◽  
Mass Activity

The present study was aimed to investigate the effects of body weight and scrotal circumference on semen production traits of 136 crossbred Jersey bulls, which were maintained at three frozen semen stations of Tamil Nadu, India. Semen production traits such as semen volume, sperm concentration, mass activity, initial sperm motility, post-thaw motility and number of doses per ejaculate were taken for this study. The overall mean values for semen volume (ml), sperm concentration (millions per ml), mass activity (0 to 5 scale), initial sperm motility (per cent), postthaw motility (per cent) and number of doses per ejaculate were 4.18 ± 0.02, 1052.80 ± 7.26, 2.09 ± 0.07, 63.36 ± 0.00, 50.52 ± 0.00 and 215.50 ± 2.01 respectively. From this study, it revealed that the body weight and scrotal circumference of bulls significantly influenced all semen production traits except mass activity. Bulls having a body weight between 350 to 550 kg produced better quality semen and more number of frozen semen doses per ejaculate. When the scrotal circumference was >36 cm, majority of the semen production traits showed better values. The body weight was positively and significantly correlated with scrotal circumference, Semen volume and number of frozen semen doses produced per ejaculate. The scrotal circumference was positively and significantly correlated with semen volume, mass activity and number of doses per ejaculate; negatively and significantly correlated with initial sperm motiltiy.

scholarly journals Semen Production and Semen Quality of Mehsana Buffalo Breed Under Semiarid Climatic Conditions of Organized Semen Station in India

2021 ◽  
Author(s):  
Nikhil Shantilal Dangar ◽  
Balkrishna P Brahmkshtri ◽  
Niteen Deshmukh ◽  
Kamlesh Prajapati
Keyword(s):  
Sperm Motility ◽  
Linear Mixed Model ◽  
Environmental Changes ◽  
Semen Quality ◽  
Random Effect ◽  
Sperm Concentration ◽  
Season Of Birth ◽  
Production Traits ◽  
Semen Collection ◽  
Semen Volume

Abstract Semen production data comprising of 55071 ejaculates of 144 bulls from Mehsana buffalo breed was analysed. The traits under study were semen volume, sperm concentration, initial sperm motility, post-thaw sperm motility and number of semen doses per ejaculate. The objective of the present study was to assess the effect of various factors affecting semen production traits and measure the semen production potential of Mehsana buffalo bulls. Data collected of semen production traits were analysed using linear mixed model, including a random effect of bull along with fixed effect of various non-genetic factors like farm, ejaculate number, season of birth, period of birth, season of semen collection and period of semen collection. First ejaculation had higher semen volume and sperm concentration resulted in to higher number of semen doses but semen quality was better in second ejaculation. Season of birth of the bull was affecting semen quality traits. As the period of birth advances semen volume increases whereas sperm concentration decreases which reflected in persistent production of number of semen doses per ejaculate. Monsoon and summer were favorable seasons for semen collection because of higher sperm concentration which resulted in to higher semen doses per ejaculate. Additionally, Monsoon collected semen had highest volume. Hence, monsoon followed by summer season would be the favorable season for semen collection. Period of semen collection affecting all the semen production traits under study but it did not have specific trend which means managemental and environmental changes over the period have sizable influence on the traits. Results of the study will help to plan future managemental practices and breeding strategies to improve semen production traits.

scholarly journals Semen Production Characteristics of Pasundan Bull at Different Body Weight

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Anggit Damaratri Lapoliwa ◽  
Nurul Isnaini
Keyword(s):  
Body Weight ◽  
Sperm Motility ◽  
Recovery Rate ◽  
Sperm Concentration ◽  
The Body ◽  
Production Traits ◽  
Motile Sperm ◽  
Frozen Semen ◽  
Semen Volume ◽  
Production Characteristics

ABSTRACT. Pasundan cattle is currently proposed as a potential livestock to support national meat self-sufficiency program. However, the information regarding their reproductive performance is still very limited. This study was conducted to evaluate the semen production characteristics of Pasundan bull at different body weight. A total of 178 semen samples which were collected from one Pasundan bull were used in this study. The semen collection was done for 31 months and during this period, the body weight of Pasundan bull was classified into 4 categories, namely 400 kg, 400 to 500 kg, 500 to 600 kg, and ≥600 kg. The results showed that overall mean semen volume, semen pH, sperm concentration, total sperm, individual sperm motility, total motile sperm, post-thawing sperm motility, recovery rate of sperm motility, and frozen semen production were 5.89 ml/ejaculate, 6.69, 1.04 billion/ml, 6.04 billion/ejaculate, 55.75%, 3.40 billion/ejaculate, 40.91%, 58.20%, and 265.11 doses/ejaculate. The difference in body weight significantly affect semen volume (P=0.001), semen pH (P=0.001), sperm concentration (P=0.043), total sperm (P=0.002), individual sperm motility (P0.001), total motile sperm (P0.001), and frozen semen production (P=0.004). There was no significant effect (P0.05) of body weight on post-thawing sperm motility and recovery rate of sperm motility. In conclusion, the semen production traits of Pasundan bull are improved with the increase in body weight up to 500 to 600 kg and remain stable at the body weight of ≥600 kg.  (Karakteristik produksi semen sapi Pasundan pada bobot badan yang berbeda) ABSTRAK. Sapi Pasundan saat ini diajukan sebagai salah satu ternak potensial untuk mendukung program swasembada daging nasional. Akan tetapi, informasi tentang penampilan reproduksinya masih sangat terbatas. Penelitian ini dilakukan untuk mengevaluasi karakteristik produksi semen sapi pejantan Pasundan pada bobot badan yang berbeda. Sebanyak 178 sampel semen yang dikoleksi dari 1 ekor sapi pejantan Pasundan digunakan pada penelitian ini. Penampungan semen dilakukan selama 31 bulan dan pada periode tersebut, bobot badan sapi pejantan Pasundan diklasifikasikan menjadi 4 kategori yaitu 400 kg, 400 to 500 kg, 500 to 600 kg, and ≥600 kg. Hasil penelitian menunjukkan bahwa rata-rata volume semen, pH semen, konsentrasi sperma, total sperma, motilitas individu sperma, total sperma motil, motilitas sperma post-thawing, nilai recovery rate, dan produksi semen beku adalah 5,89 ml/ejakulat, 6,69, 1,04 miliar/ml, 6,04 miliar/ejakulat, 55,75%, 3,40 miliar/ejakulat, 40,91%, 58,20%, dan 265,11 dosis/ejakulat. Perbedaan bobot badan memberikan pengaruh terhadap volume semen (P=0,001), pH semen (P=0,001), konsentrasi sperma (P=0,043), total sperma (P=0,002), motilitas individu sperma (P0,001), total sperma motil (P0,001), dan produksi semen beku (P=0,004). Di sisi lain, bobot badan tidak memberikan pengaruh (P0,05) terhadap motilitas sperma post-thawing dan nilai recovery rate. Kesimpulannya, karakteristik produksi semen sapi pejantan Pasundan meningkat seiring dengan peningkatan bobot badan hingga 500 sampai 600 kg dan tetap stabil hingga ≥600 kg.

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