Effect of soaking and germination on approximate composition and certain characterize of isolate protein (solubility and degree of hydrolysis) Of mung bean Vigna radiate

Four groups of local mung bean Vigna radiate were soaked for 24h. Three of these were germinated for 1, 2 and 3 days at room temperature 23˚Cusing wet cloths between technique while the fourth was depending as a soaking sample in addition to control treatment without soaking. Approximate chemical composition (crud protein, oil, ash, moisture, fibers and carbohydrates) and mung protein isolates (MPI) with its solubility SP and degree of hydrolysis DH, were determined for four dehulling groups besides control sample. Dehulling mung bean DMB flour contained 23.53, 1.83, 3.11, 7.5, 17.14 and 46.89% crud protein, oil, ash, moisture, fibers and carbohydrates, respectively. While the values of MPI, SP, and DH were 12.85, 1.5 and 1.4 % respectively .During germination significantly increasing in (CP) (MPI) and (DH) and the maximum values were 25.29, 15.34 and 2.11%respectively after two days, while maximum value was 9.53% for protein solubility after 3 days of germination. While oil content diminished a little and slight inconstant changes were shown in fibers and rest carbohydrates.

Download Full-text

Enzymatic Fractionation of Protein, Fat and Chitin from Hermetia illucens (L.) (Diptera: Stratiomyidae)

Proceedings ◽

10.3390/proceedings2019036187 ◽

2020 ◽

Vol 36 (1) ◽

pp. 187

Author(s):

Michael Josias Woods ◽

Louwrens Christiaan Hoffman ◽

Elsje Pieterse ◽

Neill Jurgens Goosen

Keyword(s):

Protein Hydrolysate ◽

Protein Solubility ◽

Amino Acid Profile ◽

Process Conditions ◽

Degree Of Hydrolysis ◽

Hermetia Illucens ◽

Hydrolysis Conditions ◽

Hydrolysis Of ◽

Central Composite ◽

By Products

Abstract: Insects have the ability to convert biowaste into valuable functional compounds, such as proteins, fat and chitin. Currently, unlike with conventional commodities, there are few fractionation methods to isolate these compounds for diversification of use. Enzymatic hydrolysis is a possible method to fractionate Hermetia illucens larvae, into protein, fat and chitin. This method is a particularly attractive due to the milder process conditions required compared to chemical methods, the relative ease to control the reaction and minimal formation of unpalatable and toxic by-products. A central composite design was used to help identify the optimum hydrolysis conditions for fractionation. At these conditions the fat recovery was ?~81%; substantially more than previous attempts and the fatty acid profile stayed unchanged from the original larvae material. The protein solubility was ~57%, with a degree of hydrolysis of ~22%, and was on par with previous studies. The amino acid profile of the soluble proteins differed from the original larvae material with a slight decrease in the ratio of essential to total amino acids. The insoluble proteins were accounted for with the chitin. A washing-and-sieving step, as means to recover the insoluble proteins from the chitin is envisaged. Also, the spray drying of the protein hydrolysate produced and its functional properties allows for future investigation. This technology allows for higher margins to be made, both environmentally as well as financially, compared to the use of the ‘intact’ biomass.

Download Full-text

Lattice imaging and pore structure of β ferric oxyhydroxide

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100108465 ◽

1978 ◽

Vol 36 (1) ◽

pp. 264-265

Author(s):

T. Baird ◽

J.R. Fryer ◽

S.T. Galbraith

Keyword(s):

Electron Microscopy ◽

Room Temperature ◽

Bulk Material ◽

Model Structure ◽

Bulk Crystals ◽

Lattice Imaging ◽

Thin Sections ◽

Ferric Oxyhydroxide ◽

Resolution Electron ◽

Hydrolysis Of

Introduction Previously we had suggested (l) that the striations observed in the pod shaped crystals of β FeOOH were an artefact of imaging in the electron microscope. Contrary to this adsorption measurements on bulk material had indicated the presence of some porosity and Gallagher (2) had proposed a model structure - based on the hollandite structure - showing the hollandite rods forming the sides of 30Å pores running the length of the crystal. Low resolution electron microscopy by Watson (3) on sectioned crystals embedded in methylmethacrylate had tended to support the existence of such pores.We have applied modern high resolution techniques to the bulk crystals and thin sections of them without confirming these earlier postulatesExperimental β FeOOH was prepared by room temperature hydrolysis of 0.01M solutions of FeCl3.6H2O, The precipitate was washed, dried in air, and embedded in Scandiplast resin. The sections were out on an LKB III Ultramicrotome to a thickness of about 500Å.

Download Full-text

Polarographic study of hydrolysis of [8-lysine]vasopressin and its derivatives with blood serum of pregnant women

Collection of Czechoslovak Chemical Communications ◽

10.1135/cccc19801099 ◽

1980 ◽

Vol 45 (4) ◽

pp. 1099-1108 ◽

Cited By ~ 1

Author(s):

Mikuláš Chavko ◽

Michal Bartík ◽

Evžen Kasafírek

Keyword(s):

Blood Serum ◽

Pregnant Women ◽

Degree Of Hydrolysis ◽

Polarographic Study ◽

Ph Optimum ◽

Lysine Vasopressin ◽

Rate Of Hydrolysis ◽

Hydrolysis Of ◽

Vasopressin Analogues

A polarographic study of the hydrolysis of [8-lysine]vasopressin and some hormonogens of the vasopressin series with the blood serum of women in the last week of pregnancy was studied. The dependence of hydrolysis on pH (pH optimum: 7.4-7.50, substrate concentration (Km 1.2 . 10-5M), pH stability and thermal stability were determined. The rate of hydrolysis of individual vasopressin analogues decreases in the order: [8-lysine]vasopressin > Nα-glycyl-prolyl[8-lysine]-vasopressin > Nα-leucyl-[8-lysine]vasopressin > Nα-alanyl-[8-lysine]vasopressin > Nα-phenyl alanyl-[8-lysine]vasopressin > Nα-diglycyl-[8-lysine]vasopressin > Nα-prolyl-[8-lysine]vasopressin > Nα-triglycyl-[8-lysine]vasopressin > Nα-sarcosyl-glycyl-[8-lysine]vasopressin. The degree of hydrolysis gradually increases to a multiple with the length of the pregnancy in consequence of the presence of oxytocine. However, vasopressin is also hydrolysed to a small extent with the enzymes from the blood sera of non-pregnant women. Under similar analytical conditions oxytocin was not hydrolysed with the sera of non-pregnant women and therefore oxytocin is a more suitable substrate than vasopressin for polarographic determination of serum oxytocinase.

Download Full-text

Process intensification for enzyme assisted turmeric starch hydrolysis in hydrotropic and supercritical conditions

International Journal of Chemical Reactor Engineering ◽

10.1515/ijcre-2020-0161 ◽

2021 ◽

Vol 0 (0) ◽

Author(s):

Yogita P. Labrath ◽

Prafulla V. Belge ◽

Uma G. Kulkarni ◽

Vilas G. Gaikar

Keyword(s):

Residence Time ◽

Filtration Rate ◽

Room Temperature ◽

Curcuma Longa ◽

Process Intensification ◽

Enzyme Treatment ◽

Starch Hydrolysis ◽

Reaction Conditions ◽

Natural Form ◽

Hydrolysis Of

Abstract The turmeric rhizome (Curcuma longa) contains curcuminoids embedded in the starch matrix. It is thus important to target starch hydrolysis to enhance extraction of curcuminoids. In the case of starch hydrolysis, α-amylase is more efficient when the starch is in a gelatinised form than when it is in its natural form. The present work includes hydrolysis of turmeric starch in its natural and gelatinised forms using α-amylase in hydrotrope solution (HS) and scCO2. The optimum rate of starch hydrolysis was obtained using 200 IU cm−3 of α-amylase, at reaction conditions of 6.5 pH at 328 K when 10% w/w of turmeric powder was stirred at 900 rpm in HSs. The hydrolysis in 15 MPa scCO2 at room temperature required a phase modifier and 40 min of residence time (RT). The enzyme treatment of turmeric powder in HSs increased the filtration rate for curcuminoid extraction (gelatinised and native) compared to untreated turmeric powder.

Download Full-text

The Effect of Bacterial R. liguminosarum and Fungal T. harzinum Inoculation and Organic Manure on Root Nodes, Growth, and Yield of Mung Bean, Vigna radiate

IOP Conference Series Earth and Environmental Science ◽

10.1088/1755-1315/735/1/012050 ◽

2021 ◽

Vol 735 (1) ◽

pp. 012050

Author(s):

Huda Rasim Jawad Al-Shabani ◽

Jawad Abdel-Kadhim Kamal

Keyword(s):

Mung Bean ◽

Organic Manure ◽

Growth And Yield ◽

Vigna Radiate

Download Full-text

Interaction of Bioactive Mono-Terpenes with Egg Yolk on Ice Cream Physicochemical Properties

Foods ◽

10.3390/foods10081686 ◽

2021 ◽

Vol 10 (8) ◽

pp. 1686

Author(s):

Mostafa Gouda ◽

Long Sheng ◽

Rana Muhammad Aadil ◽

Yuanyuan Liu ◽

Meihu Ma ◽

...

Keyword(s):

Multivariate Analysis ◽

Water Distribution ◽

Structural Characteristics ◽

Viscosity Index ◽

Control Sample ◽

Ice Cream ◽

Egg Yolk ◽

Control Treatment ◽

Non Invasive ◽

Magnetic Resonance Imaging Mri

Using natural multi-function phytochemicals could be one of the best solutions for clean-label production. In this study, dairy ice creams were prepared containing 14% egg yolk and 0.1% of thymol (THY), trans-cinnamaldehyde (TC), menthol (MEN), or vanillin (VAN). Then, the physical, chemical, and structural characteristics were evaluated. Magnetic resonance imaging (MRI) analysis (a rapid, chemical-free, and non-invasive tool) was carried out to evaluate the water distribution. A multivariate analysis was conducted among all studied variables. According to the results, the overrun of the MEN ice cream was significantly increased as compared to the control sample. The density was also reduced in the MEN sample. Meanwhile, the spreadability (%) of VAN was significantly increased after 6 min as compared to the control treatment. MRI analysis revealed that water distribution was significantly changed in the THY group. The firmness and viscosity of THY samples were significantly increased (p < 0.05). Multivariate analysis indicated that viscosity index and consistency were the top parameters affected by THY. The authors concluded that THY and VAN are promising stabilizers for ice-cream clean production.

Download Full-text

Research on Enzymatic Hydrolysis of Whey Protein

Applied Mechanics and Materials ◽

10.4028/www.scientific.net/amm.411-414.3205 ◽

2013 ◽

Vol 411-414 ◽

pp. 3205-3209

Author(s):

Fang Qian ◽

Lei Zhao ◽

Shu Juan Jiang ◽

Guang Qing Mu

Keyword(s):

Enzymatic Hydrolysis ◽

Whey Protein ◽

Degree Of Hydrolysis ◽

Quadratic Regression ◽

Orthogonal Regression ◽

Single Factor Analysis ◽

Verification Test ◽

Enzyme Dosage ◽

Hydrolysis Conditions ◽

Hydrolysis Of

Based on single factor analysis for the enzymatic hydrolysis of whey protein, papain was selected as the optimal enzyme and its enzymatic hydrolysis conditions were optimized by the quadratic regression orthogonal rotary test. The orthogonal regression model for degree of hydrolysis (DH) to three factors including temperature (X1), time (X2), enzyme dosage (X3) was established as follow: DH=10.40+0.22X1+0.30X2+1.31X3+0.019X1X2+0.011X1X3-0.039X2X3-0.39X12-0.16X22-0.40X32, Verification test showed a DH of 11.7% was obtained at the optimal hydrolysis condition of 56.6°C, 113.8 min and enzyme 8213.7 U /g protein, which basically consisted with the model theoretical value.

Download Full-text

The synthesis of Cyclododecane-r-1,c-5,c-9-triamine and r-1,c-5,c-9-Tris(2,3-dimethoxybenzamido)cyclododecane

Australian Journal of Chemistry ◽

10.1071/ch9750673 ◽

1975 ◽

Vol 28 (3) ◽

pp. 673 ◽

Cited By ~ 5

Author(s):

DJ Collins ◽

C Lewis ◽

JM Swan

Keyword(s):

Aqueous Solution ◽

Acid Hydrolysis ◽

Sulphuric Acid ◽

Sodium Carbonate ◽

Sodium Azide ◽

Room Temperature ◽

Dimethyl Formamide ◽

Lithium Aluminium Hydride ◽

Lithium Aluminium ◽

Hydrolysis Of

Treatment of cyclododecane-r-1,c-5,c-9-triyl tris(p-toluenesulphonate) with sodium azide in dimethyl-formamide at 100� for 6 h gave the corresponding cis,cis-triazide which upon hydrogenation or reduction with lithium aluminium hydride gave cyclododecane-r-1,c-5,c-9-triamine, isolated as the tris-salicylidene derivative. Acid hydrolysis of this, removal of the salicylaldehyde, and treatment of the aqueous solution with sodium carbonate and 2,3-dimethoxybenzoyl chloride gave r-1,c-5,c- 9-tris(2,3-dimethoxybenzamido)cyclododecane. �� Treatment of (E,E,E)-cyclododeca-1,5,9-triene with an excess of acetonitrile and sulphuric acid at room temperature for three days gave 18% of (E,E)-1-acetamidocyclododeca-4,8-diene; no di- or tri-amides were isolated.

Download Full-text