scholarly journals The Oral Cavity: A Reservoir that Favors Colonization and Selection of Candida parapsilosis sensu stricto Strains with High Pathogen Potential Under Conditions of Gingival-periodontal Disease

2018 ◽  
Vol 2 (1) ◽  
pp. 1-9 ◽  
Author(s):  
María L Rodríguez
Keyword(s):  
Oral Cavity ◽  
Periodontal Disease ◽  
Candida Parapsilosis ◽  
Sensu Stricto ◽  
Selection Of

scholarly journals Oral Dysbiosis Exacerbates Candida parapsilosis Sensu Stricto Biofilm Production via Up-Regulation of the CPH2 Biofilm Master Gene

2021 ◽  
pp. 1-7
Author(s):  
Rodríguez ML ◽  
Rodríguez ML ◽  
Rosa AC ◽  
Nastri ML ◽  
Jewtuchowicz VM
Keyword(s):  
Oral Cavity ◽  
Metabolic Activity ◽  
Transcriptional Activity ◽  
Candida Parapsilosis ◽  
Sensu Stricto ◽  
Basal Expression ◽  
Pathological Conditions ◽  
Biofilm Production ◽  
Confocal Fluorescence ◽  
Nosocomial Candidemia

Introduction: Candida parapsilosis sensu stricto is the second to third most frequent cause of candidemia. Studies place this yeast as a frequent colonizer of niches of the oral cavity, predominantly in pathological conditions. We hypothesize that a buccal environment in dysbiosis enhances the virulence of C. parapsilosis sensu stricto. Objective: To evaluate the phenotype and molecular level of the production of biofilm in oral isolates of Candida parapsilosis sensu stricto and correlate the results with the clinical origin (dysbiosis versus eubiosis). Materials and Methods: The biofilm-forming ability was compared in 50 oral isolates of Candida parapsilosis sensu stricto obtained from patients with and without oral dysbiosis; by quantification of metabolic activity. The results were corroborated by confocal fluorescence microscopy, and correlated with the transcriptional activity of CPH2, by RT-qPCR. The data were analysed by Excel 2010, and InfoStat 2018, with a 95% confidence interval. Results: The metabolic activity in biofilm was significantly higher in oral dysbiosis relative to control (p = 0.0025). Basal expression of CPH2 increased 2.8 times more in oral dysbiosis related to the control condition and showed no significant differences with pathogenic isolates of this same yeast, derived from onychomycosis lesions. Conclusion: The oral cavity in dysbiosis increases the virulence of C. parapsilosis sensu stricto due to possible changes in epigenetic marks. This finding suggests that the oral cavity in dysbiosis may be an alternative route to the skin in the epidemiology of nosocomial candidemia.

scholarly journals Oral dysbiosis exacerbates the virulence of Candida parapsilosis sensu stricto via up-regulation of the CPH2 biofilm master gene

2021 ◽  
Vol 10 (1) ◽  
pp. 01-011
Author(s):  
Rodríguez María Lourdes ◽  
Rosa Alcira Cristina ◽  
Nastri María Lorena ◽  
Jewtuchowicz Virginia Martha
Keyword(s):  
Oral Cavity ◽  
Metabolic Activity ◽  
Transcriptional Activity ◽  
Candida Parapsilosis ◽  
Molecular Level ◽  
Sensu Stricto ◽  
Basal Expression ◽  
Pathological Conditions ◽  
Confocal Fluorescence ◽  
Nosocomial Candidemia

Candida parapsilosis sensu stricto is the second to third most frequent cause of candidemia. Studies place this yeast as a frequent colonizer of niches of the oral cavity, predominantly in pathological conditions. We hypothesize that a buccal environment in dysbiosis enhances the virulence of C. parapsilosis sensu stricto. Objective: To evaluate at the phenotype and molecular level the production of biofilm in oral isolates of C. parapsilosis sensu stricto and correlate the results with the clinical origin (dysbiosis versus eubiosis). Material and methods: The biofilm-forming ability was compared in 50 oral isolates of C. parapsilosis sensu stricto obtained from patients with and without oral dysbiosis; by quantification of biofilm biomass and metabolic activity. The results were corroborated by optical and confocal fluorescence microscopy, and correlated with the transcriptional activity of CPH2, by RT-qPCR. The data were analyzed by Excel 2010, and InfoStat 2018, with a 95% confidence interval. Results: The metabolic activity in biofilm was significantly higher in oral dysbiosis relative to control (p = 0.0025). Basal expression of CPH2 increased 2.8 times more in oral dysbiosis related to the control condition and showed no significant differences with pathogenic isolates of this same yeast, derived from onychomycosis lesions. Conclusion: The oral cavity in dysbiosis increases the virulence of C. parapsilosis sensu stricto due to possible changes in epigenetic marks. This finding suggests that the oral cavity in dysbiosis may be an alternative route to the skin in the epidemiology of nosocomial candidemia.

scholarly journals The oral cavity promotes virulence of Candida parapsilosis sensu stricto via up-regulation of BCR1

2020 ◽  
Vol 6 (1) ◽  
pp. 120-133
Author(s):  
Rodríguez María Lourdes ◽  
Alcaraz Eliana Sabrina ◽  
Rosa Alcira Cristina ◽  
Jewtuchowicz Virginia Marta
Keyword(s):  
Oral Cavity ◽  
Candida Parapsilosis ◽  
Sensu Stricto

scholarly journals THE STUDY OF THE EFFECTIVENESS OF USING THE CERTAIN TOOTHPASTES WITH DIFFERENT ACTIVE INGREDIENTS

2018 ◽  
Vol 25 (2) ◽  
pp. 57-61
Author(s):  
S. B. Ulitovsky ◽  
A. V. Antipova
Keyword(s):  
Oral Cavity ◽  
Periodontal Disease ◽  
Oral Hygiene ◽  
Active Ingredients ◽  
Proper Selection ◽  
Dental Diseases ◽  
Anti Inflammatory ◽  
Degrees Of Severity ◽  
Prevention Of Dental Diseases ◽  
Selection Of

Introduction. Nowadays, the prevention of dental diseases is very important in dentistry. The main measure aimed at prevention of dental diseases is an individual oral hygiene. Its effectiveness depends on the quality and regularity, as well as the proper selection of means of individual oral hygiene.The objective is to study the effectiveness of using the certain toothpastes with different active ingredients.Material and methods. Toothpastes «А» and «B» were used to study the effectiveness of using the certain toothpastes with different active ingredients. Probantes at the age of 50 to 70 years, with the presence of dental deposits and periodontal disease of moderate and severe degrees of severity took part in the study of the effectiveness of using these toothpastes. They were divided into two groups of 26 people in each group. The study evaluated the following indices: the hygiene index of Green – Vermillion (1964), gingival PMA index (Parma, 1960), the bleeding index of Muhleman and Son (1971). Results. The experiment discovered the decrease of the values of the digital indicators of the hygiene index of Green – Vermillion, gingival PMA index, and the bleeding index of Muhleman and Son in all groups during the whole period. These changes of the values of the indices were accompanied with the simultaneously increase in the values of a cleansing effect, anti-inflammatory and hemostatic efficiency.Conclusions. Toothpastes «А» and «В» are treatment-and-prophylactic toothpastes, acting effectively on the tissues and organs of the oral cavity with prolonged use. 

scholarly journals A case series of medically managed Candida parapsilosis complex prosthetic valve endocarditis

2021 ◽  
Vol 20 (1) ◽  
Author(s):  
Penghao Guo ◽  
Yuting He ◽  
Rui Fan ◽  
Zhongwen Wu ◽  
Yili Chen ◽  
...  
Keyword(s):  
Prosthetic Valve ◽  
Candida Parapsilosis ◽  
Case Series ◽  
Prosthetic Valve Endocarditis ◽  
Sensu Stricto ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Candida Metapsilosis ◽  
Candida Orthopsilosis ◽  
Tof Ms

Abstract Background In recent years, Candida parapsilosis is recognized as a species complex and is composed of Candida parapsilosis sensu stricto, Candida orthopsilosis and Candida metapsilosis. Candida parapsilosis complex prosthetic valve endocarditis (PVE) is rare and the survival rate is still low despite of optimal therapeutic strategies. In our report, it is novel to report cases as Candida parapsilosis complex PVE at species and identify Candida parapsilosis using MALDI-TOF MS. Case presentation A series of 4 cases of Candida parapsilosis complex PVE from our institution was reported. Three were infected by Candida parapsilosis sensu stricto and one was infected by Candida metapsilosis. The condition of two cases got better and the other died. Conclusions More attention should be paid to Candida parapsilosis complex PVE and early diagnosis and prompt antibiotic therapy may play a role in the treatment for Candida parapsilosis complex PVE. It is recommended to identify Candida parapsilosis complex at species level and MALDI-TOF MS as an easy, fast and efficient identification method is worth promoting in clinical microbiology

scholarly journals The occurrence of Entamoeba gingivalis among patients with periodontal disease

2016 ◽  
Vol 29 (2) ◽  
pp. 86-89 ◽  
Author(s):  
Joanna Luszczak ◽  
Michal Bartosik ◽  
Jolanta Rzymowska ◽  
Agnieszka Sochaczewska-Dolecka ◽  
Ewa Tomaszek ◽  
...  
Keyword(s):  
Oral Cavity ◽  
Periodontal Disease ◽  
Periodontal Diseases ◽  
Age Groups ◽  
Healthy People ◽  
Gingival Tissue ◽  
Pathological Changes ◽  
Oral Diseases

AbstractAccording to some studies, the Entamoeba gingivalis colonizing the gingival tissue is an important agent in bringing about periodontitis. Other studies, however, deem it an opportunist that is able to survive in the medium induced by periodontal disease. The aim of this study was to investigate the prevalence of Entamoeba gingivalis infection in patients from the Department of Periodontology, and compare this population with that of healthy people, so as to analyze the relationships between infection and patient sex and age. The result of this work is that in both groups, a correlation (p = 0,19) has been noted between the occurrence of amoebae and other diseases in the oral cavity. Indeed, 81,4% of all patients with some periodontal disease showed the presence of amoeba. Among those who are not afflicted with oral diseases, the presence of amoeba was indicated in 62,5% of the total. In addition, a correlation between the person's age and the presence of protozoa (p = 0,15) was strongly marked among women (p = 0,19). In the three age groups of women in this study (40-49, 60-69, and above 80 years), we observed a 100% presence of protozoa.Our study leads us to the conclusion that infections with Entamoeba gingivalis should be regarded as an factor that is associated with the pathological changes occurring in patients with periodontal diseases.

The bacteriome of the oral cavity in healthy dogs and dogs with periodontal disease

2022 ◽  
pp. 1-9
Author(s):  
Brook A. Niemiec ◽  
Jerzy Gawor ◽  
Shuiquan Tang ◽  
Aishani Prem ◽  
Janina A. Krumbeck
Keyword(s):  
Oral Cavity ◽  
Periodontal Disease ◽  
Bacterial Species ◽  
Abundant Species ◽  
Rrna Gene ◽  
Core Microbiome ◽  
The Core ◽  
Severe Periodontal Disease ◽  
Healthy Dogs ◽  
V3 Region

Abstract OBJECTIVE To compare the bacteriome of the oral cavity in healthy dogs and dogs with various stages of periodontal disease. ANIMALS Dogs without periodontal disease (n = 12) or with mild (10), moderate (19), or severe (10) periodontal disease. PROCEDURES The maxillary arcade of each dog was sampled with a sterile swab, and swabs were submitted for next-generation DNA sequencing targeting the V1–V3 region of the 16S rRNA gene. RESULTS 714 bacterial species from 177 families were identified. The 3 most frequently found bacterial species were Actinomyces sp (48/51 samples), Porphyromonas cangingivalis (47/51 samples), and a Campylobacter sp (48/51 samples). The most abundant species were P cangingivalis, Porphyromonas gulae, and an undefined Porphyromonas sp. Porphyromonas cangingivalis and Campylobacter sp were part of the core microbiome shared among the 4 groups, and P gulae, which was significantly enriched in dogs with severe periodontal disease, was part of the core microbiome shared between all groups except dogs without periodontal disease. Christensenellaceae sp, Bacteroidales sp, Family XIII sp, Methanobrevibacter oralis, Peptostreptococcus canis, and Tannerella sp formed a unique core microbiome in dogs with severe periodontal disease. CONCLUSIONS AND CLINICAL RELEVANCE Results highlighted that in dogs, potential pathogens can be common members of the oral cavity bacteriome in the absence of disease, and changes in the relative abundance of certain members of the bacteriome can be associated with severity of periodontal disease. Future studies may aim to determine whether these changes are the cause or result of periodontal disease or the host immune response.

The mycobiome of the oral cavity in healthy dogs and dogs with periodontal disease

2022 ◽  
pp. 1-8
Author(s):  
Brook A. Niemiec ◽  
Jerzy Gawor ◽  
Shuiquan Tang ◽  
Aishani Prem ◽  
Janina A. Krumbeck
Keyword(s):  
Oral Cavity ◽  
Periodontal Disease ◽  
Dna Sequencing ◽  
Fungal Species ◽  
Next Generation ◽  
Data Set ◽  
Next Generation Dna Sequencing ◽  
Sequencing Platform ◽  
The Family ◽  
Healthy Dogs

Abstract OBJECTIVE To investigate the mycobiome of the oral cavity in healthy dogs and dogs with various stages of periodontal disease. ANIMALS 51 dogs without periodontal disease (n = 12) or with mild (10), moderate (19), or severe (10) periodontal disease. PROCEDURES The whole maxillary arcade of each dog was sampled with a sterile swab, and swabs were submitted for next-generation DNA sequencing targeting the internal transcribed spacer 2 region with a commercial sequencing platform. RESULTS Fungi were detected in all samples, with a total of 320 fungal species from 135 families detected in the data set. No single fungal species was found in all samples. The 3 most frequently found fungal species were Cladosporium sp (46/51 samples), Malassezia restricta (44/51 samples), and Malassezia arunalokei (36/51 samples). Certain fungi, specifically those of the family Didymellaceae, the family Irpicaceae, and the order Pleosporales, were significantly associated with different stages of periodontitis. Mycobial analysis indicated that Cladosporium sp could be considered part of the core oral cavity mycobiome. CONCLUSIONS AND CLINICAL RELEVANCE Results highlighted that fungi are present in the oral cavity of dogs and are characterized by substantial species diversity, with different fungal communities associated with various stages of periodontal disease. The next-generation DNA sequencing used in the present study revealed substantially more species of fungi than previous culture-based studies.

scholarly journals Identification and selection of non-Saccharomyces strains isolate from brazilian apple must

2018 ◽  
Vol 48 (5) ◽  
Author(s):  
Giovana Arruda Moura Pietrowski ◽  
Juliana Vitória Messias Bittencourt ◽  
Luciana Rocha Brandão ◽  
Carlos Augusto Rosa ◽  
Aline Alberti ◽  
...  
Keyword(s):  
Candida Parapsilosis ◽  
Pichia Anomala ◽  
Pcr Analysis ◽  
Hanseniaspora Uvarum ◽  
Candida Oleophila ◽  
Aroma Production ◽  
Fuji Apple ◽  
Saccharomyces Yeasts ◽  
Selection Of

ABSTRACT: This study aimed to know the biodiversity of non-Saccharomyces yeasts in Brazilian apples must with potential to improve of the aromatic quality of ciders. The strains were isolated from thirty-five (35) Gala and Fuji apple musts from different locations from south region of Brazil. Forty-five (45) strains were isolate and identified by PCR analysis. Results indicated ten (10) species: Candida oleophila, Candida parapsilosis, Candida tropicalis, Clavispora lusitaniae, Hanseniaspora guilliermondii, Hanseniaspora uvarum, Lodderomyces elongisporus, Pichia anomala, Pichia fermentans and Rhodotorula mucilaginosa. The genus Rhodotorula sp., Lodderomyces sp. and Clavispora sp. constituted 71.2% of the strains identified. The following strains, C. oleophila, R. mucilaginosa, P. fermentans, H. uvarum and H. guilliermondii were selected in qualitative tests due the fruity aroma production by trained team in the aromatic assessment of cider.

Changing Patterns in the Late Pleistocene/Early Holocene Prehistory of Southern Africa as Seen from the Nelson Bay Cave Stone Artifact Sequence

1978 ◽  
Vol 10 (1) ◽  
pp. 84-111 ◽  
Author(s):  
Janette Deacon
Keyword(s):  
Southern Africa ◽  
Sensu Stricto ◽  
Middle Stone Age ◽  
Stone Age ◽  
Faunal Remains ◽  
Later Stone Age ◽  
Large Numbers ◽  
Changing Patterns ◽  
Selection Of ◽  
Stone Artifact

The dating of the Stone Age sequence in southern Africa has been considerably revised over the last decade, and one of the anomalies which has resulted is that the Middle Stone Age, now dated to beyond 30,000 B.P., does not immediately precede the Later Stone Agesensu stricto. The excavation and analysis of occupation horizons dating between the most recent Middle Stone Age assemblages and the Holocene is therefore of particular interest. Nelson Bay Cave, situated on the southern coast of South Africa, contains deposits which partly fill the “gap” between the Middle and Later Stone Ages, and the occupation horizons dating between about 18,000 and 5000 years ago are described in this paper. Changes in the habitat in the vicinity of the site caused by sea-level and vegetation changes coincident with the amelioration of temperatures at the end of the Pleistocene are clearly marked in the faunal remains at the site. Largely correlated with the faunal changes (which includes the introduction of marine resources to the cave at about 12,000 B.P.) are changes in the stone artifact assemblages. Three industries are recognized in the sequence: the Robberg, characterized by microbladelets produced from bladelet cores and a few small scrapers and backed tools; the Albany, characterized by large scrapers and an absence of backed tools; and the Wilton, characterized by a variety of Formal Tools including relatively large numbers of small scrapers and backed tools. These changes in artifact-manufacturing traditions are interpreted as signaling adjustments to changing environmental conditions. An explanation for these adjustments is not sought in a simple cause-and-effect relationship between the environment and the cultural response; artifact changes are seen instead as the result of a twofold process, with the environment acting as an external stimulus to change, and the direction of the artifact change governed by the selection of a range of possibilities offered by the technology of the Later Stone Agesensu latothat was widespread in subequatorial Africa during the last 20,000 years.

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