scholarly journals TOTAL PHENOLIC CONTENTS, ANTIOXIDANT, ANTICANCER AND ANTIDIABETIC PROPERTIES OF Myrmecodia tuberosa (RUBIACEAE)

2013 ◽  
Vol 9 (3) ◽  
pp. 2000-2004
Author(s):  
Saidi Rasemi ◽  
Dr Heng Yen Khong ◽  
Rohaya Ahmad ◽  
Mizaton Hazizul Hasan
Keyword(s):  
Ethyl Acetate ◽  
Ethanolic Extract ◽  
Bark Extract ◽  
Total Phenolic ◽  
Cytotoxicity Test ◽  
Phenolic Contents ◽  
Total Phenolic Contents ◽  
Hela Cell Lines ◽  
Ic50 Value ◽  
Cancer Agent

Myrmecodia tuberosa, locally known as “Sarang Semut”, belongs to the Rubiaceae family. The aim of the study is to investigate the total phenolic contents and bioactivity of the crude extract of M. tuberosa. The tuber, bark and leaves of the plant were cut, washed and air-dried. The plant was extracted with ethyl acetate and ethanol to yield EtOAc and EtOH crudes. The crudes were then tested for antioxidant 2,2-diphenyl-1-(2,4,6-trinitrophenyl)hydrazyl (DPPH) assay, α-glucosidase assay for anti-diabetic activity and [3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium] MTT Assay for cytotoxicity test. The results showed that bark ethanolic extract demonstrated the highest DPPH scavenging of 95.16% while other crudes were ranging from 92.77% to 94.55% scavenging of DPPH compared to ascorbic acid. The tuber ethyl acetate extract demonstrated the highest inhibition of α -glucosidase enzyme with 72.58% while other crudes were ranging from 63.27% to 72.46% inhibition. In addition, the ethanolic tuber extract exhibited the strongest cytotoxicity against Human colorectal Cancer (HT-29) and Cervical Cancer (HeLa) cell lines with the IC50 value of 16 µg/mL and 14 µg/mL respectively. Meanwhile, the ethanolic bark extract exhibited the strongest cytotoxicity against Human Breast Cancer (MCF-7) with IC50 value of 6.0µg/mL. Furthurmore, the tuber extracts contained the highest phenolic content with 1087mg GAE/ g extract compared to the leaves and barks of the plants. The findings suggest that the whole part of M. tuberosa is a potential natural source for anti-oxidative, anti-diabetic and anti-cancer agent.

scholarly journals Loranthus pulverulentus: A Potent Source of Natural Antioxidants and Alternative Medicine

2013 ◽  
Vol 2013 ◽  
pp. 1-7 ◽  
Author(s):  
Muhammad Asam Raza ◽  
Rukhsana Kausar ◽  
Faraz Ali Rana ◽  
Muhammad Danish ◽  
Durre Shahwar ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Natural Antioxidants ◽  
Stem Bark ◽  
Antioxidant Potential ◽  
Total Phenolic ◽  
Strong Correlations ◽  
Phenolic Contents ◽  
Total Phenolic Contents ◽  
Total Antioxidant

This study was designed to evaluate the antioxidant potential ofLoranthus pulverulentus. Stem bark, leaves, and seeds ofLoranthus pulverulentuswere extracted in methanol:water (90 : 10) and partitioned with n-hexane, chloroform, ethyl acetate, and n-butanol successively using partition chromatography. Total phenolic contents and antioxidant potential were checked using standard protocols. Total phenolic contents of all extracts were determined, using Folin–Ciocalteu reagent, and ranged between 151 ± 2.1 and 396 ± 1.6 for stem bark, 137 ± 0.9 and 430 ± 2.2 for, and 39 ± 0.6 and 231 ± 1.7 for seeds. The antioxidant potential of extracts was evaluated; namely, DPPH, FRAP, and total antioxidant models. The ethyl acetate extract of stem-bark, leaves, and seeds showed the highest activity in DPPH (94.5 ± 2.1%, 96.30 ± 0.9%, and 92.30 ± 1.1%, IC5015.9 ± 0.5 μg, 14.5 ± 0.8, and 102.7 ± 1.3, resp.), FRAP (7.7 ± 0.6, 7.5 ± 0.7 and 6.6 ± 0.7, resp.), and total antioxidant (0.95 ± 0.09, 1.19 ± 0.09, and 0.686 ± 0.08, resp.). Strong correlations were observed between total phenols versus total antioxidant activity, DPPH, and FRAP withR2values ranging from 0.8185 to 0.9951 (stem-bark), 0.6728 to 0.8648 (leaves), and 0.8658 to 0.9910 (seed) which indicated that phenolic contents are the major constituents responsible for antioxidant activity.

SKRINING FITOKIMIA DAN UJI AKTIVITAS ANTIOKSIDAN DAN ANTITUMOR 20 TUMBUHAN OBAT ASAL KABUPATEN MANOKWARI

2008 ◽  
Vol 7 (1) ◽  
Author(s):  
Bimo Budi Santoso
Keyword(s):  
Antioxidant Activity ◽  
Medicinal Plants ◽  
Antitumor Activity ◽  
Traditional Method ◽  
Extraction Efficiency ◽  
Phenolic Content ◽  
Antioxidant Activities ◽  
Total Phenolic ◽  
Phenolic Contents ◽  
Total Phenolic Contents

<strong><em>Flavanoid, steroid, and terpenoid contents of 20 Manokwari medicinal plants were analyzed.  The antioxidant activity, total phenolic contents and antitumor activity of 20 Manokwari medicinal plants were also evaluated. The result shows that 83% positif to flavanoid test, 59% positif to terpenoid test and only 25% positif steroid. Antioxidant activity and total phenolic contents evaluated using Ferric Thiocianate (FTC) and Folin-Ciocalteu methods respectively. Antioxidant activity and total phenolic contents of medicinal plants were extracted by the traditional method, boiling in water and also in 80% methanol. Twenty plants evaluated in both exstracts have significantly varies of antioxidant activities and phenolic contents,  A significant and linier correlation coefficient between the antioxidant activity and the total phenolic content was found in both aqueous (R<sup>2</sup>= 0,77) and methanol (R<sup>2</sup> = 0,85). Antitumor activity was tested using cell maurine P-388 and only 2 of medicinal plants are active to inhibit cell maurine P-388. Comparing extraction efficiency of the two methods, the methanol extracted phenolic compounds more efficiently, and antioxidant activity of the extract was higher.</em></strong>

scholarly journals Nutritional Composition of Solid-State Fermented Camelina Meal (An Enriched Protein Source for Broiler Chickens)

Proceedings ◽  
2020 ◽  
Vol 36 (1) ◽  
pp. 106 ◽  
Author(s):  
Oladapo Oluwaseye Olukomaiya ◽  
Chrishanthi Fernando ◽  
Ram Mereddy ◽  
Xiuhua Li ◽  
Yasmina Sultanbawa
Keyword(s):  
Solid State ◽  
Phytic Acid ◽  
Crude Protein ◽  
Total Sugar ◽  
Poultry Feed ◽  
Total Phenolic ◽  
Oilseed Crop ◽  
Phenolic Contents ◽  
Total Phenolic Contents ◽  
Camelina Meal

Camelina (Camelina sativa) also known as false flax or gold of pleasure is an oilseed crop of the Brassica (Cruciferae) family. Camelina is not a food crop, however, the by-product (meal or cake) obtained from the oil extraction of camelina seeds is useful as animal feed because of its moderate crude protein content. The dietary use of camelina meal in broiler diets is limited to low inclusion due to the presence of anti-nutritional factors such as fibre, phytic acid, glucosinolates and tannins which have negative effects on broiler performance. Solid-state fermentation (SSF) is a suitable processing method for enriching agroindustrial by-products since it offers several cost-effective and practical advantages. In the present study, the effect of SSF on the nutrient composition, phytic acid and total phenolic contents of expeller-extracted camelina meal was evaluated. Aspergillus ficuum (ATCC 66876) was used for SSF under aerobic conditions at 30oC for 7 days. Unfermented and fermented camelina meals were analyzed for dry matter, crude protein, crude fat, crude fibre, total sugar (sucrose) and starch as well as for pH, phytic acid and total phenolic contents. Crude protein was improved by 6.79% while total sugar and starch were reduced by 90.99% and 75.78%, respectively in the solid-state fermented camelina meal. Phytic acid and total phenolic contents were also decreased by 39.17% and 56.11%, respectively. This study revealed that SSF could be used to improve the nutritional quality of camelina meal for improved use in poultry feed formulation.

scholarly journals Bioactive Compounds, Antioxidant, and Antibacterial Properties of Lonicera caerulea Berries: Evaluation of 11 Cultivars

Plants ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 624
Author(s):  
Laima Česonienė ◽  
Juozas Labokas ◽  
Ina Jasutienė ◽  
Antanas Šarkinas ◽  
Vilma Kaškonienė ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Vitamin C ◽  
Organic Acids ◽  
Bioactive Compounds ◽  
Total Phenolic ◽  
Aqueous Extracts ◽  
Lonicera Caerulea ◽  
Phenolic Contents ◽  
Total Phenolic Contents ◽  
Ethanolic Extracts

The aim of the study was to evaluate 11 cultivars of blue honeysuckle (Lonicera caerulea L.) for bioactive compounds, antioxidant capacity, and the antibacterial activity of berries. Total phenolic contents (TPCs) and total anthocyanin contents (TACs) were established by using ethanolic extracts. For contents of organic acids and saccharides, aqueous extracts were used, and vitamin C was determined by using oxalic acid solution. DPPH• radical scavenging capacity was evaluated by using ethanolic extracts; antibacterial activity was assessed by using both ethanolic and aqueous extracts. The TPC varied from 364.02 ± 0.41 mg/100 g in ‘Vostorg’ to 784.5 ± 0.3 mg/100 g in ‘Obilnaja’, and TAC ranged from 277.8 ± 1.1 mg/100 g in ‘Čelnočnaja’ to 394.1 ± 8.4 mg/100 g in ‘Nimfa’. Anthocyanins comprised 53.8% of total phenolic contents on average. Among organic acids, citric acid was predominant, averaging 769.41 ± 5.34 mg/100 g, with malic and quinic acids amounting to 289.90 ± 2.64 and 45.00 ± 0.37 mg/100 g on average, respectively. Contents of vitamin C were 34.26 ± 0.25 mg/100 g on average. Organic acids were most effective in the inhibition of both Gram-positive and Gram-negative bacteria tested. In conclusion, berries of L. caerulea are beneficial not only for fresh consumption, but also as a raw material or ingredients of foods with high health-promoting value.

scholarly journals Antioxidant, Anti-Diabetic and Phytochemical Screening of Dried Ficus carica Linn Fruit Powder

2020 ◽  
Vol 2 (1) ◽  
pp. 50
Keyword(s):  
Ethyl Acetate ◽  
Phenolic Content ◽  
Ethyl Acetate Extract ◽  
Total Phenolic ◽  
Total Flavonoid Content ◽  
Phytochemical Screening ◽  
Flavonoid Content ◽  
Dpph Assay ◽  
Ic50 Value ◽  
Different Types

Ficus carica L. or the common name is red figs that belong to the Moraceae family or the Mulberry family. Every part of the fruit or the tree has been able to treat different types of common diseases, for example, it is used as a natural laxative or a supplementary food for diabetes. Thus, this research uses four different solvents, ethyl acetate, ethanol, hexane, and water, to obtain the respective crude extracts in order to investigate the anti-diabetic properties by determining the inhibition of the activity of the diabetic enzymes, α-amylase, and α-glucosidase; and the DPPH assay determines the antioxidant activity while qualitative phytochemical screening was for phenols, alkaloids, tannins, saponins, and flavonoids; total quantitative phenolic and total flavonoid content was done. The phytochemical screening showed the presence of the compounds tested in different types of crude extracts. For the total phenolic content, ethyl acetate extract exhibits the highest content. In contrast, hexane extract shows the highest total flavonoid content. For the DPPH assay, ethyl acetate extract has the highest scavenging activity at 13.351 µg/mL with corresponding with the data of total phenolic content. For the α-glucosidase inhibitory activity, water extract has the lowest IC50 value among the four extracts but higher value than the standard. For α-amylase inhibitory activity, only ethanol extract showed the IC50 value, but it was a high value. In conclusion, there is potential for figs to be a natural source of medicine, and the extracts tested can be used for future studies.

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