scholarly journals CHINESE CABBAGE BrMYB34.2 TRANSCRIPTION FACTOR REGULATES INDOLIC GLUCOSINOLATES BIOSYNTHESIS IN Arabidopsis

2020 ◽  
Vol 19 (1) ◽  
pp. 85-95
Author(s):  
Ye Zhao ◽  
Yongqiang Zhang ◽  
Xianfeng Guo ◽  
Yan Ma ◽  
Peng Zhang ◽  
...  
Keyword(s):  
Transgenic Plants ◽  
Chinese Cabbage ◽  
Ectopic Expression ◽  
Plant Secondary Metabolites ◽  
Orthologous Gene ◽  
Phenotypic Analysis ◽  
Stress Treatment ◽  
Real Time Quantitative Pcr ◽  
Aliphatic Glucosinolates ◽  
Key Genes

Glucosinolates (GS) are a group of sulfur- and nitrogen-rich plant secondary metabolites that originate fromamino acids and exist mainly in plants in the order Brassicales, such as Arabidopsis thaliana (Arabidopsis) and Chinese cabbage (Brassica rapa ssp. pekinensis). To date, several regulatory components responsible for GS biosynthesis have been identified in Arabidopsis. However, the functions of GS biosynthesis regulators in Chinese cabbage have not been clarified. In our current study, a putative ATR1/MYB34 orthologous gene, BrMYB34.2, was isolated from Chinese cabbage leaves. To investigate the function of this gene, we engineered Arabidopsis plants that overexpress BrMYB34.2 ectopically and phenotypic analysis was performed. Moreover, we assayed the accumulation levels of indolic GS (IGS) and aliphatic glucosinolates in transgenic plants and test the expression of key genes of IGS biosynthesis and tryptophan synthesis by Real-time quantitative PCR. And further analysed the resistance of transgenic plants in 5MT stress treatment. The results indicate that ectopic expression of the BrMYB34.2 gene in Arabidopsis was able to up-regulate the accumulation level of IGS due to the increased expression of IGS and Trp biosynthetic genes. Moreover, overexpression of BrMYB34.2 conferred Arabidopsis 5MT resistance. These results suggest that the BrMYB34.2 gene may function as one of the regulators of IGS and Trp biosynthesis in Chinese cabbage.

Ectopic expression of a R2R3 MYB transcription factor of dove tree (Davidia involucrata) aggravates seed abortion in Arabidopsis thaliana

2020 ◽  
Vol 47 (5) ◽  
pp. 454
Author(s):  
Jian Li ◽  
Tian Chen ◽  
Fengzhen Huang ◽  
Penghui Dai ◽  
Fuxiang Cao ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Transcription Factor ◽  
Transgenic Plants ◽  
Ectopic Expression ◽  
Myb Transcription Factor ◽  
Seed Abortion ◽  
Transgenic Seeds ◽  
Genes Encoding ◽  
Colour Changes ◽  
R2r3 Myb

Serious seed abortion of dove tree (Davidia involucrate Baill.) is one of the critical factors leading to the low fecundity of this species. Seed abortion is a complicated process and various factors have been verified to synergistically determine the fate of seeds. To reveal the mechanism of seed abortion in D. involucrata, we performed transcriptome analysis in normal and abortive seeds of D. involucrata. According to the transcriptome data, we noticed that most of the genes encoding a MYB transcription factor were predominantly expressed in abortive seeds. Among these, a gene named DiMYB1 was selected and its function was validated in this study. Overexpression of DiMYB1 resulted in obviously reduced viability of transgenic seeds and seedlings, and caused a significantly higher seed abortion rate. The vegetative growth of transgenic plants was hindered, resulting in an earlier flowering time. In addition, colour changes occurred in transgenic plants. Some transgenic sprouts, stems and pods appeared purple instead of green in colour. Our finding demonstrated that DiMYB1 participates in multiple plant developmental processes, especially in seed development in Arabidopsis thaliana (L.) Heynh., which indicated the similar role of this gene in D. involucrata.

scholarly journals REGIA, An EU Project on Functional Genomics of Transcription Factors fromArabidopsis thaliana

2002 ◽  
Vol 3 (2) ◽  
pp. 102-108 ◽  
Author(s):  
Javier Paz-Ares ◽  
The REGIA Consortium
Keyword(s):  
Transcription Factors ◽  
Expression Patterns ◽  
Regulatory Gene ◽  
Gene Array ◽  
Ectopic Expression ◽  
Phenotypic Analysis ◽  
Systematic Analysis ◽  
Biotechnological Potential ◽  
Reading Frame ◽  
Eu Project

Transcription factors (TFs) are regulatory proteins that have played a pivotal role in the evolution of eukaryotes and that also have great biotechnological potential. REGIA (REgulatory Gene Initiative in Arabidopsis) is an EU-funded project involving 29 European laboratories with the objective of determining the function of virtually all transcription factors from the model plant,Arabidopsis thaliana. REGIA involves: 1. the definition ofTFgene expression patterns inArabidopsis; 2. the identification of mutations atTFloci; 3. the ectopic expression of TFs (or derivatives) inArabidopsisand in crop plants; 4. phenotypic analysis of the mutants and mis-expression lines, including both RNA and metabolic profiling; 5. the systematic analysis of interactions between TFs; and 6. the generation of a bioinformatics infrastructure to access and integrate all this information. We expect that this programme will establish the full biotechnological potential of plant TFs, and provide insights into hierarchies, redundancies, and interdependencies, and their evolution. The project involves the preparation of both aTFgene array for expression analysis and a normalised full length open reading frame (ORF) library of TFs in a yeast two hybrid vector; the applications of these resources should extend beyond the scope of this programme.

Ectopic expression of a rice transcription factor, Mybleu, enhances tolerance of transgenic plants of Carrizo citrange to low oxygen stress

2011 ◽  
Vol 109 (2) ◽  
pp. 327-339 ◽  
Author(s):  
Paola Caruso ◽  
Elena Baldoni ◽  
Monica Mattana ◽  
Donata Pietro Paolo ◽  
Annamaria Genga ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Transgenic Plants ◽  
Ectopic Expression ◽  
Low Oxygen ◽  
Oxygen Stress ◽  
Carrizo Citrange

scholarly journals Demonstration of improved plant growth and biomass production by At1g 73160 promoter-controlled root-preferential expression of AtGA20ox gene

2020 ◽  
Author(s):  
Linh Khanh Ly ◽  
Thao Phuong Bui ◽  
Phat Tien Do ◽  
Anh Van Thi Le ◽  
Phong Van Nguyen ◽  
...  
Keyword(s):  
Transgenic Plants ◽  
Plant Growth ◽  
Transgenic Tobacco ◽  
Plant Species ◽  
Leaf Shape ◽  
Ectopic Expression ◽  
Constitutive Expression ◽  
Stem Length ◽  
Melia Azedarach ◽  
Significant Difference

Abstract Background: Overexpression of GA20 oxidase gene has been a recent trend for improving plant growth and biomass. Constitutive expression of GA20ox has successfully improved plant growth and biomass in several plant species. However, the constitutive expression of this gene causes side-effects, such as reduced leaf size and stem diameters, etc. To avoid these effects, different tissue-specific promoters had been identified and employed for GA20ox overexpression. In this study, we demonstrate the potential of At1g, a root-preferential promoter, for GA20ox expression to enhance plant biomass in tobacco and Melia azedarach.Results: We examined the utility of At1g promoter to drive the expression of GUS (β-1,4-glucuronidase) reporter and GA20ox genes in tobacco and Melia azedarach. Histochemical GUS assays in tobacco showed that At1g was a root-preferential promoter whose expression was particularly strong in root tips. The ectopic expression of AtGA20ox gene under the control of At1g promoter showed the improved plant growth and biomass of both tobacco and M. azedarach transgenic plants compared to wild-type (WT) control plants. Stem length as well as stem and root fresh weights increased by up to 1.5-3 folds in transgenic tobacco and 2 folds in transgenic M. azedarach. Both tobacco and M. azedarach transgenic plants showed increases in the root xylem width, xylem over phloem ratio by 50%-100% as compared to WT plants. Importantly, no significant difference in the leaf shape and size was observed between At1g::AtGA20ox transgenic and WT plants. Moreover, transgenic M. azedarach showed a 135% increase in stem diameter even though no change was found in transgenic tobacco.Conclusions: These results demonstrate the great utility of At1g promoter, when driving AtGA20ox gene, for growth and biomass improvements in woody plants and potentially some other plant species.

scholarly journals Identification of the Eutrema Salsugineum EsMYB90 gene important for anthocyanin biosynthesis

2019 ◽  
Author(s):  
Yuting Qi ◽  
Caihong Gu ◽  
Xingjun Wang ◽  
Shiqing Gao ◽  
Changsheng Li ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Transgenic Plants ◽  
Anthocyanin Biosynthesis ◽  
Ectopic Expression ◽  
Myb Transcription Factor ◽  
Anthocyanin Synthesis ◽  
Eutrema Salsugineum ◽  
Tobacco Transgenic Plants ◽  
Myb Genes ◽  
Anthocyanin Production

Abstract Background: Anthocyanins contribute to coloration and antioxidation effects in different plant tissues. MYB transcription factors have been demonstrated to be a key regulator for anthocyanin synthesis in many plants. However, little information was available about the MYB genes in the halophyte species Eutrema salsugineum.Result: Here we report the identification of an important anthocyanin biosynthesis regulator EsMYB90 from Eutrema salsugineum, which is a halophyte tolerant to multiple abiotic stresses. Our phylogenetic and localization analyses supported that EsMYB90 is an R2R3 type of MYB transcription factor. Ectopic expression of EsMYB90 in tobacco and Arabidopsis enhanced pigmentation and anthocyanin accumulation in various organs. The transcriptome analysis revealed that 42 genes upregulated by EsMYB90 in 35S:EsMYB90 tobacco transgenic plants are required for anthocyanin biosynthesis. Moreover, our qRT-PCR results showed that EsMYB90 promoted expression of early (PAL, CHS, and CHI) and late (DFR, ANS, and UFGT) anthocyanin biosynthesis genes in stems, leaves, and flowers of 35S:EsMYB90 tobacco transgenic plants.Conclusions: Our results indicated that EsMYB90 is a novel MYB transcription factor, which regulates anthocyanin biosynthesis genes to control anthocyanin biosynthesis. Our work provides a new tool to enhance anthocyanin production in various plants.

SCL Oncogene Perturbs HEB/E2A Function on Thymocyte Differentiation.

Blood ◽  
2005 ◽  
Vol 106 (11) ◽  
pp. 236-236
Author(s):  
Mathieu Tremblay ◽  
Sabine Herblot ◽  
Marianne Desrosiers ◽  
Margarita Todorova ◽  
Eric Paquet ◽  
...  
Keyword(s):  
T Cell ◽  
Target Genes ◽  
Cell Transformation ◽  
Ectopic Expression ◽  
Global Gene Expression ◽  
Leukemic Cells ◽  
Wild Type ◽  
Real Time Quantitative Pcr ◽  
Helix Loop Helix ◽  
Thymocyte Differentiation

Abstract The SCL and LMO oncogenes are frequently activated in childhood T cell acute leukemia (T-ALL). SCL is a transcription factor of the basic helix-loop-helix (bHLH) family that forms heterodimers with other members of the family, specifically HEB and E2A. SCL can activate or repress transcription but the mechanism through which SCL functions as an oncogene remains to be clarified. Ectopic expression of SCL and LMO in the thymus of transgenic mice causes thymocyte differentiation arrest during the preleukemic phase with aberrant differentiation at the DN3-DN4 stage, prior to the acquisition of CD4 and CD8. We therefore took several approaches to define the mechanism underlying differentiation arrest in these cells. We first analyzed global gene expression of pre-leukemic DN3 thymocytes from SCLtg/LMOtg mice against their wild type littermates. We found that in this context, these oncogenes act as global transcriptional repressor as 90% of the genes with more than two fold differences are repressed when compared to wild type controls. Furthermore, we identify the HEB/E2A pathway as being targeted by SCL/LMO and used different approaches to show that the HEB/E2A activity is repressed by these oncogenes. First, using real-time quantitative PCR, we confirmed the repression of known HEB/E2A target genes (pTa and Rag1/2). In addition, we identified new HEB/E2A target genes that were confirmed as direct target through chromatin immunoprecipitation of primary thymocytes. This array also reveals that SCL associates with HEB and/or E2A on DNA to repress their function. Furthermore, we took a genetic approach to show that SCL/LMO collaborates with HEB haploinsufficiency in inducing leukemia. Our observations therefore reveal that the repression of the HEB pathway is crucial for T cell transformation. The importance of this repression is underscored by the fact that the E2A/HEB target genes that we identify here are expressed at low levels in primary leukemic cells from T-ALL patients when compared to B-ALL or AML samples. Together, these results show that SCL/LMO repress HEB/E2A activity to block T cell differentiation, an important step for T cell leukemia.

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