scholarly journals Osteoprotective Effects of ‘Anti-Diabetic’ Polyherbal Mixture in Type 1 Diabetic Rats

2021 ◽  
Vol 71 (3) ◽  
pp. 256-272
Author(s):  
Aleksandra Petrović ◽  
Višnja Madić ◽  
Marina Jušković ◽  
Ljubiša Đorđević ◽  
Perica Vasiljević
Keyword(s):  
Bone Loss ◽  
Diabetic Rats ◽  
Female Rats ◽  
Histopathological Changes ◽  
Healthy Animal ◽  
Histological Changes ◽  
Time Treatment ◽  
Single Intraperitoneal Injection ◽  
Alloxan Monohydrate

Abstract Bone loss leading to osteopenia and osteoporosis is a frequent secondary complication of diabetes. This study aimed to evaluate the value of a traditionally used ‘anti-diabetic’ polyherbal mixture as a possible remedy for the prevention of this complication. Diabetes was induced in Wistar female rats with a single intraperitoneal injection of alloxan monohydrate. The animals with blood glucose higher than 20 mmol/L for 14 consecutive days were considered diabetic. For the next 14 days, animals were treated with two concentrations of the polyherbal mixture (10 and 20 g of dry plant material/ kg). Bone histopathology was evaluated using the H&E and Masson’s trichrome staining. Alloxan-induced diabetes triggered bone histological changes characteristic for the development of osteopenia and osteoporosis and treatment with the polyherbal decoction restored these histopathological changes of the bones to the healthy animal level. At the same time, treatment with these tested doses has shown no adverse effects. These findings suggest that this mixture might be used as a remedy for the prevention of diabetic bone loss.

scholarly journals The Effects of Lycopene and Insulin on Histological Changes and the Expression Level of Bcl-2 Family Genes in the Hippocampus of Streptozotocin-Induced Diabetic Rats

2017 ◽  
Vol 2017 ◽  
pp. 1-9 ◽  
Author(s):  
Masoume Soleymaninejad ◽  
Seyed Gholamali Joursaraei ◽  
Farideh Feizi ◽  
Iraj Jafari Anarkooli
Keyword(s):  
Cell Death ◽  
Wistar Rats ◽  
Diabetic Rats ◽  
Expression Level ◽  
Histological Changes ◽  
Group D

The aim of this study was to evaluate the effects of antioxidants lycopene and insulin on histological changes and expression of Bcl-2 family genes in the hippocampus of streptozotocin-induced type 1 diabetic rats. Forty-eight Wistar rats were divided into six groups of control (C), control treated with lycopene (CL), diabetic (D), diabetic treated with insulin (DI), diabetic treated with lycopene (DL), and diabetic treated with insulin and lycopene (DIL). Diabetes was induced by an injection of streptozotocin (60 mg/kg, IP), lycopene (4 mg/kg/day) was given to the lycopene treated groups as gavages, and insulin (Sc, 1-2 U/kg/day) was injected to the groups treated with insulin. The number of hippocampus neurons undergoing cell death in group D had significant differences with groups C and DIL (p<0.001). Furthermore, insulin and lycopene alone or together reduced the expression of Bax, but increased Bcl-2 and Bcl-xL levels in DI, DL, and DIL rats, especially when compared to group D (p<0.001). The ratios of Bax/Bcl-2 and Bax/Bcl-xL in DI, DL, and DIL rats were also reduced (p<0.001). Our results indicate that treatment with insulin and/or lycopene contribute to the prevention of cell death by reducing the expression of proapoptotic genes and increasing the expression of antiapoptotic genes in the hippocampus.

Isoflavones improve collagen I and glycosaminoglycans and prevent bone loss in type 1 diabetic rats

Climacteric ◽  
2019 ◽  
Vol 23 (1) ◽  
pp. 75-83
Author(s):  
A. A. F. Carbonel ◽  
M. C. Vieira ◽  
R. S. Simões ◽  
P. D. A. Lima ◽  
L. F. P. Fuchs ◽  
...  
Keyword(s):  
Bone Loss ◽  
Diabetic Rats ◽  
Collagen I ◽  
Prevent Bone Loss

scholarly journals Baccharis trimera (Carqueja) Improves Metabolic and Redox Status in an Experimental Model of Type 1 Diabetes

2018 ◽  
Vol 2018 ◽  
pp. 1-12 ◽  
Author(s):  
Natália Nogueira do Nascimento Kaut ◽  
Ana Carolina Silveira Rabelo ◽  
Glaucy Rodrigues Araujo ◽  
Jason Guy Taylor ◽  
Marcelo Eustáquio Silva ◽  
...  
Keyword(s):  
Type 1 Diabetes ◽  
Superoxide Dismutase ◽  
Glutathione Peroxidase ◽  
Diabetic Rats ◽  
Female Rats ◽  
Redox Stress ◽  
Carbonyl Protein ◽  
Hydroethanolic Extract ◽  
Baccharis Trimera

Diabetes mellitus is a metabolic disorder that causes severe complications due to the increased oxidative stress induced by disease. Many plants are popularly used in the treatment of diabetes, e.g., Baccharis trimera (carqueja). The aim of this study was to explore the potential application of the B. trimera hydroethanolic extract in preventing redox stress induced by diabetes and its hypoglycemic properties. Experiments were conducted with 48 female rats, divided into 6 groups, named C (control), C600 (control + extract 600 mg/kg), C1200 (control + extract 1200 mg/kg), D (diabetic), D600 (diabetic + 600 mg/kg), and D1200 (diabetic + 1200 mg/kg). Type 1 diabetes was induced with alloxan, and the animals presented hyperglycemia and reduction in insulin and body weight. After seven days of experimentation, the nontreated diabetic group showed changes in biochemical parameters (urea, triacylglycerol, alanine aminotransferase, and aspartate aminotransferase) and increased carbonyl protein levels. Regarding the antioxidant enzymes, an increase in superoxide dismutase activity was observed but in comparison a decrease in catalase and glutathione peroxidase activity was noted which suggests that diabetic rats suffered redox stress. In addition, the mRNA of superoxide dismutase, catalase, and glutathione peroxidase enzymes were altered. Treatment of diabetic rats with B. trimera extract resulted in an improved glycemic profile and liver function, decreased oxidative damage, and altered the expression of mRNA of the antioxidants enzymes. These results together suggest that B. trimera hydroethanolic extract has a protective effect against diabetes.

scholarly journals Boldine Does Not Modify Gender Specific Wound Healing in Zucker Diabetic Rats

2018 ◽  
Vol 13 (11) ◽  
pp. 1934578X1801301
Author(s):  
Renata Köhlerová ◽  
Eva Čermáková ◽  
Milena Hajzlerová
Keyword(s):  
Type 2 Diabetes ◽  
Diabetic Rats ◽  
Female Rats ◽  
Male Rats ◽  
Positive Effects ◽  
Natural Alkaloid ◽  
Anti Inflammatory ◽  
Antioxidant Effects

Boldine is a natural alkaloid with anti-inflammatory and antioxidant effects. It reduces glycemia and decreases blood pressure in rats with type 1 diabetes. We have also studied whether boldine has anti-inflammatory and antioxidant effects in rats with type 2 diabetes and whether it can improve healing of their skin wounds, a serious comorbidity of type 2 diabetes. This work also compares lean and obese Zucker diabetic rats, including a comparison of both sexes. After skin excisions, the wounded animals received granules containing boldine ad libitum. The weights of rats, amount of consumed food and wound size were measured regularly. Scar and internal organs were removed and analyzed. Further, the tensile strength of the scar was tested and hydroxyproline content (a marker of collagen) measured. We have not confirmed previously published positive effects of boldine, but we observed important differences between gender and between genotypes. Male rats had higher body weight, liver, kidney and spleen than female rats. Furthermore, their wounds took longer to heal with bigger scar areas.

scholarly journals Dendrobium officinalePrevents Early Complications in Streptozotocin-Induced Diabetic Rats

2016 ◽  
Vol 2016 ◽  
pp. 1-10 ◽  
Author(s):  
Shao-zhen Hou ◽  
Chu-yan Liang ◽  
Hua-zhen Liu ◽  
Dong-mei Zhu ◽  
Ya-yun Wu ◽  
...  
Keyword(s):  
Blood Glucose ◽  
Chinese Herb ◽  
Serum Levels ◽  
Diabetic Rats ◽  
Preventive Effect ◽  
Early Complications ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Single Intraperitoneal Injection

Background. Dendrobium officinale(DO) Kimura et Migo is a precious Chinese herb that is considered beneficial for health due to its antioxidant and antidiabetes properties, and so on. In this research, we try to determine the preventive effect of DO on the early complications of STZ-induced diabetic rats.Methods. Type 1 diabetic rats were produced with a single intraperitoneal injection of STZ (50 mg/kg). DO (1 g/kg/day) was then orally administered for 5 weeks. Blood glucose, TC, TG, BUN, CREA, and GSH-PX levels were determined, and electroretinographic activity and hypoalgesia were investigated. Pathological sections of the eyes, hearts, aortas, kidneys, and livers were analyzed.Results. Treatment with DO significantly attenuated the serum levels of TC, TG, BUN, and CREA, markedly increased the amplitudes of ERG a- and b-waves and Ops, and reduced the hypoalgesia and histopathological changes of vital organs induced by hyperglycemia. The protective effect of DO in diabetic rats may be associated with its antioxidant activity, as evidenced by the marked increase in the serum level of glutathione peroxidase. However, DO had no significant effect on blood glucose levels and bodyweight of diabetic rats.Conclusions. DO supplementation is an effective treatment to prevent STZ-induced diabetic complications.

scholarly journals Histological changes of female reproductive organs subjected to different jumping exercise intensities and honey supplementation in rats

PeerJ ◽  
2019 ◽  
Vol 7 ◽  
pp. e7646
Author(s):  
Maryam Mosavat ◽  
Mahaneem Mohamed ◽  
Foong Kiew Ooi ◽  
Mitra Mirsanjari ◽  
Anani Aila Mat Zin ◽  
...  
Keyword(s):  
Body Weight ◽  
High Intensity ◽  
Reproductive Organs ◽  
High Intensity Exercise ◽  
Female Rats ◽  
Corpora Lutea ◽  
Histopathological Changes ◽  
Histological Changes ◽  
Jumping Exercise ◽  
Female Reproductive Organs

Background We assessed histopathological changes of ovaries and uterus in female rats subjected to different jumping exercise intensities combined with honey supplementation at one g/kg body weight/day. Methods A total of 72 rats were divided into six groups, 12 rats in each: control (C), 20 and 80 jumps (20E, 80E), honey (H), and 20 and 80 jump with honey (20EH, 80EH). Results The endometrium was significantly thicker in the rats in H, 20EH and 80EH groups compared to C, 20E, and 80E. The myometrium thickness was significantly lower in 80E and significantly higher in 80EH compared to C, respectively. There was significantly higher myometrium thickness in 20EH and 80EH compared to 20E and 80E and H. The number of glands of the uterus in 20E and 80E was significantly lower than C. However, there was a significantly higher number of glands in H, 20EH, and 80EH compared to 20E and 80E. The numbers of uterus vessels were significantly lower in 80E compared to 20E. However, the numbers of vessels were significantly higher in H, 20EH, and 80EH compared to 80E. The number of ovarian haemorregia was significantly lower in 20E, 80E, H, 20EH, and 80EH compared to C. The number of corpora lutea was significantly lower in 80EH, H, 80E, and 20E compared to C. However, the number of corpora lutea was significantly higher in 20EH compared to J20 and H. Conclusion This study suggested that jumping exercises in particularly high-intensity exercise may induce histopathological changes in uterus and ovary in rats, and honey supplementation may ameliorate these effects.

Ocular thrombosis and retinal degeneration induced in female F344 rats by 2-butoxyethanol

1999 ◽  
Vol 18 (9) ◽  
pp. 577-582 ◽  
Author(s):  
A Nyska ◽  
R R Maronpot ◽  
B I Ghanayem
Keyword(s):  
Blood Vessels ◽  
Microscopic Examination ◽  
Female Rats ◽  
Female Rat ◽  
Photoreceptor Degeneration ◽  
Histopathological Changes ◽  
Ocular Tissues ◽  
Histological Changes ◽  
Ciliary Processes ◽  
F344 Rats

2-butoxyethanol, used extensively for domestic and industrial purposes, was tested in our experiments for its potential to cause damage to female rat ocular tissues. Female rats were previously found to be particularly sensitive to 2-butoxyethanol. A group of eight female F344 rats (2–3 months old) were exposed by gavage to 250 mg of 2-butoxyethanol/kg b.w. per day for 3 consecutive days and sacrificed 24 h after the last dose. Eight female rats received the dosing vehicle (water) and served as controls. At necropsy, petechial hemorrhages were noted on the sclera. Microscopic examination revealed treatmentrelated effects in the eyes, in addition to other known effects of BE exposure such as disseminated thrombosis and necrosis and infarction in various organs. The spectrum of histopathological changes noted in the eyes included hemorrhages localized in the posterior layers of the retina, leading to photoreceptor degeneration. Thrombi were identified in ciliary processes and limbal blood vessels. Histological changes suggestive of the retinal ischemic-infarctive process were also noted. Possible pathogenic mechanisms of 2-butoxyethanol-induced retinopathy are discussed.

scholarly journals Temporal changes in the exercise pressor reflex in type 1 diabetic rats

2017 ◽  
Vol 313 (4) ◽  
pp. H708-H714 ◽  
Author(s):  
Ann-Katrin Grotle ◽  
Elizabeth A. Garcia ◽  
Yu Huo ◽  
Audrey J. Stone
Keyword(s):  
Type 1 Diabetes ◽  
Diabetic Rats ◽  
Female Rats ◽  
Male Rats ◽  
Male And Female ◽  
Exercise Pressor Reflex ◽  
Hindlimb Muscles ◽  
Male And Female Rats ◽  
Pressor Reflex

Previous studies have shown that diabetic peripheral neuropathy affects both unmyelinated and myelinated afferents, similar to those evoking the exercise pressor reflex. However, the effect of type 1 diabetes (T1DM) on this reflex is not known. We examined, in decerebrate male and female T1DM [streptozotocin (STZ)] and healthy control (CTL) rats, pressor and cardioaccelerator responses to isometric contraction of the hindlimb muscles during the early and late stages of the disease. STZ (50 mg/kg) was injected to induce diabetes, and experiments were conducted at 1, 3, and 6 wk after injection. On the day of the experiment, we statically contracted the hindlimb muscles by stimulating the sciatic nerve and measured changes in mean arterial pressure and heart rate. We found that the pressor but not cardioaccelerator response was exaggerated in STZ rats at 1 wk (STZ: 21 ± 3 mmHg, n = 10, and CTL: 14 ± 2 mmHg, n = 10, P < 0.05) and at 3 wk (STZ: 26 ± 5 mmHg, n = 10, and CTL: 17 ± 3 mmHg, n = 11, P < 0.05) after injection. However, at 6 wk, and only in male rats, both the pressor (STZ: 13 ± 3 mmHg, n = 12, and CTL: 17 ± 3 mmHg, n = 13, P < 0.05) and cardioaccelerator responses (STZ: 7 ± 3 beats/min, n = 12, and CTL: 10 ± 3 beats/min, n = 13, P < 0.05) to contraction were significantly attenuated in STZ rats compared with CTL rats. These data indicate that T1DM exaggerates the exercise pressor reflex during the early stages of the disease in both male and female rats. Conversely, T1DM attenuates this reflex in the late stage of the disease in male but not female rats. NEW & NOTEWORTHY This is the first study to provide evidence that the pressor and cardioaccelerator responses to skeletal muscle contraction vary depending on the duration of type 1 diabetes.

scholarly journals Therapeutic Efficacy of Ficus Glomerata Leaves in Alloxan Monohydrate Induced Type-1 Diabetes in Albino Rats

2021 ◽  
Vol 5 (4) ◽  
pp. 01-02
Author(s):  
Sanjeev Kumar ◽  
N.K. Prasad ◽  
Kumari Shachi
Keyword(s):  
Blood Glucose ◽  
Insulin Treatment ◽  
Diabetic Rats ◽  
Albino Rats ◽  
Insulin Production ◽  
Glucose Lowering ◽  
Glucose Lowering Effect ◽  
Alloxan Monohydrate ◽  
Ficus Glomerata

Present study was aimed to evaluate the anti-diabetic efficacy of Ficus glomerata in Alloxan monohydrate induced albino rats. Oral administration of fresh Ficus glomerataleaves (2 - 4 g/day) for 60 days shows significant blood glucose lowering effect in experimental alloxan induced diabetic rats. These diabetic rats blood glucose level became normal when fed with Ficus glomerata leaves. It is amply revealed that fresh Ficus glomerata indica leaves possessed anti-diabetic properties. The results suggest that statistically significant anti-diabetic potential in alloxan monohydrate induced diabetic rats. The Ficus glomerata leaves to be almost similar effect like insulin treatment in alloxan monohydrate administered animal model. From the present investigation it appeared that Ficus glomerata leaves might have some ingredients to increase the output of insulin by binding to the receptors of the Beta cells of the Langerhans located in the pancreas. Once they bind to the Sulphonyl urea receptors, the K+ -ATP channels are probably closed and therefore the membrane is depolarized and insulin production is stimulated.

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