scholarly journals Histological changes of female reproductive organs subjected to different jumping exercise intensities and honey supplementation in rats

PeerJ ◽  
2019 ◽  
Vol 7 ◽  
pp. e7646
Author(s):  
Maryam Mosavat ◽  
Mahaneem Mohamed ◽  
Foong Kiew Ooi ◽  
Mitra Mirsanjari ◽  
Anani Aila Mat Zin ◽  
...  
Keyword(s):  
Body Weight ◽  
High Intensity ◽  
Reproductive Organs ◽  
High Intensity Exercise ◽  
Female Rats ◽  
Corpora Lutea ◽  
Histopathological Changes ◽  
Histological Changes ◽  
Jumping Exercise ◽  
Female Reproductive Organs

Background We assessed histopathological changes of ovaries and uterus in female rats subjected to different jumping exercise intensities combined with honey supplementation at one g/kg body weight/day. Methods A total of 72 rats were divided into six groups, 12 rats in each: control (C), 20 and 80 jumps (20E, 80E), honey (H), and 20 and 80 jump with honey (20EH, 80EH). Results The endometrium was significantly thicker in the rats in H, 20EH and 80EH groups compared to C, 20E, and 80E. The myometrium thickness was significantly lower in 80E and significantly higher in 80EH compared to C, respectively. There was significantly higher myometrium thickness in 20EH and 80EH compared to 20E and 80E and H. The number of glands of the uterus in 20E and 80E was significantly lower than C. However, there was a significantly higher number of glands in H, 20EH, and 80EH compared to 20E and 80E. The numbers of uterus vessels were significantly lower in 80E compared to 20E. However, the numbers of vessels were significantly higher in H, 20EH, and 80EH compared to 80E. The number of ovarian haemorregia was significantly lower in 20E, 80E, H, 20EH, and 80EH compared to C. The number of corpora lutea was significantly lower in 80EH, H, 80E, and 20E compared to C. However, the number of corpora lutea was significantly higher in 20EH compared to J20 and H. Conclusion This study suggested that jumping exercises in particularly high-intensity exercise may induce histopathological changes in uterus and ovary in rats, and honey supplementation may ameliorate these effects.

scholarly journals Stress Hormone and Reproductive System in Response to Honey Supplementation Combined with Different Jumping Exercise Intensities in Female Rats

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
Maryam Mosavat ◽  
Foong Kiew Ooi ◽  
Mahaneem Mohamed
Keyword(s):  
Body Weight ◽  
Serum Cortisol ◽  
Reproductive Organs ◽  
Female Rats ◽  
Stress Hormone ◽  
Serum Progesterone ◽  
Beneficial Effects ◽  
Sedentary Group ◽  
Significant Difference ◽  
Jumping Exercise

This study was performed to determine the effects of 8-week honey supplementation combined with different jumping exercise intensities on serum cortisol, progesterone, estradiol, and reproductive organs. Eighty-four 9-week-old female rats were divided into 7 groups: baseline controls (C0), sedentary group (C), 20 and 80 jumps per day (Ex20J,Ex80J), honey (H), and combined honey with 20 and 80 jumps per day (HEx20J,HEx80J) groups. Jumping exercise was performed at 5 days/week and honey was given at a dosage of 1 g/kg body weight/day for 7 days/week. The level of serum cortisol was higher inEx20JandEx80Jcompared to C. There was significantly lower value of serum cortisol inHEx20Jcompared toEx80J. Serum progesterone levels were significantly lower inEx20JandEx80Jcompared to C. However, serum progesterone levels were significantly higher inHEx20JandHEx80Jcompared toEx20JandEx80J. Relative uterine weights were significantly greater inHEx20Jcompared to C andHEx80J, respectively. There was no significant difference in estradiol level and relative ovarian weights among all the groups. Therefore, honey elicited beneficial effects in reducing the increase of cortisol and in increasing the reduce of progesterone levels induced by different intensities jumping exercise in female rats.

Effect of Exposure to Mixture of Heavy Metals Arsenic, Cadmium and Lead on Reproductive Function and Oxidative Stress in Female Rats

2017 ◽  
Vol 9 (03) ◽  
Author(s):  
Choudhuri D. ◽  
Bhattacharjee T.
Keyword(s):  
Oxidative Stress ◽  
Heavy Metals ◽  
Ascorbic Acid ◽  
Reproductive Function ◽  
Reproductive Organs ◽  
Female Rats ◽  
Corpora Lutea ◽  
Oestrus Cycle ◽  
Implantation Sites ◽  
Live Fetus

Background : Toxicological consequences arising from exposure to mixtures of heavy metals especially at low, chronic and environmentally relevant doses are poorly recognised. In the present study, we evaluated effects of chronic exposure to combinations of three metals arsenic (As), cadmium (Cd) and lead (Pb) present frequently in drinking water on reproductive function and oxidative damage caused to reproductive organs of female rats. Method : Female rats were exposed to mixture of metals (As, Cdand Pb) for 90 consecutive days. The gain in body weight and weight of reproductive organs were recorded following autopsy on 91 stday. The oestrus cycle were monitored during entire treatment period. Numbers of corpora lutea, implantation sites, live fetus and survival of the fetus were evaluated in rats mated successfully with untreated male after completion of their respective treatment. Ovarian cholesterol, protein, ascorbic acid and enzyme Δ 5 -3β HSD levels were estimated. Serum levels of steroid hormones oestrogen and progesterone were estimated. Histopathological picture of both ovary and uterus were assessed. Levels of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidise (GPX) activity, amount of reduced glutathione (GSH) and malondyaldehyde (MDA) in blood, ovary and uterus were measured as biomarkers of oxidative stress. Results : The treated rats showed reduced body weight gain and reduction in the weight of ovary and uterus. Oestrus cycle was disrupted with continuous diestrous in treated animals. Number of corpora lutea, implantation sites and live fetus and the survival of fetus evaluate were reduced significantly in treated groups. The levels of ovarian cholesterol and ascorbic acid increased in treated rats with decrease Δ5 -3β HSD level. There was reduction in serum level of both the ovarian steroid hormones oestrogen and progesterone. The protein levels did not differ between the groups. There was a significant increase in levels of MDA and decrease in levels of all the antioxidant enzymes in treated group. Conclusion : The results revealed there was disruption to reproductive functions with decrease in stereoidogenic activity and associated oxidative stress in female rats treated with combination of mixture of metals (Cd, As and Pb) at low dose for 90 consecutive days.

scholarly journals Reproductive toxicity of triazole fungicides cyproconazole and epoxiconazole when exposed to male and female wistar rats during gametogenesis

2021 ◽  
Vol 54 (1) ◽  
pp. 52-61
Author(s):  
NR Shepelskaya ◽  
YaV Kolyanchuk
Keyword(s):  
Body Weight ◽  
Reproductive Toxicity ◽  
Reproductive Function ◽  
Female Rats ◽  
Male Rats ◽  
Corpora Lutea ◽  
Disruptive Effect ◽  
Male And Female ◽  
Two Samples ◽  
Adverse Effect Level

Aim. Studying the effect of generic pesticides cyproconazole (98 %) and two samples of epoxiconazole (epoxiconazole 1 — 95,75 % and epoxiconazole 2 — 98,7 %) on the reproductive system of male and female Wistar Han rats at the level of the organism when exposed during gametogenesis, identification and characterization of their hazard, as well as assessment of the risk of reproductive toxicity of these compounds. Materials and Methods. The test samples were administered daily (5 days a week) by oral gavage at doses of 0.2 and 2.0 mg/kg for cyproconazole and 0.5 and 2.0 mg/kg for epoxiconazoles during 11 weeks for males, and 10 weeks for females. Also, there were kept intact males and females, intended for crossover mating with experimental animals. After the end of the exposure, functional indicators of the state of the gonads and the ability of animals to reproduce offspring were studied. The duration and the frequency of each stage of the estrous cycle in female rats and the number of motile sperm, the total amount of sperm and the number of abnormal forms of germ cells of the male rats were studied. The reproductive function state in females was evaluated on day 20th of pregnancy. Thereby the number of corpora lutea in the ovaries, number of alive, dead and resorbed foetuses and embryos, the foetus weight, total weight of litters were registered. The studies were carried out in accordance with the recommendations of the Bioethics Commission and the Centre’s standard operating procedures, developed in accordance with the recommendations and requirements of Good Laboratory Practice (GLP). Conclusions. Test substances at a maximum dose of 2.0 mg/kg of body weight have reproductive toxicity and endocrine-disruptive effect, exerting a significant antiandrogenic effect on males and antiestrogenic effect on female rats. No-observed-adverse-effect-level (NOАEL) for gonadal and reproductive toxicity for male and female Wistar Han rats were established. They are 0.2 mg/kg body weight for cyproconazole and 0.5 mg/kg body weight for epoxiconazole. Key Words: azole fungicides, cyproconazole, epoxiconazole, reproductive toxicity, antiandrogenic and antiestrogenic effects, Wistar Han rats.

Toxic Effects of Aluminium on Female Reproductive System in Presence of Ethanol Coexposure

2021 ◽  
pp. 3809-3815
Author(s):  
Buddhadeb Ghosh ◽  
Ravi Kant Sharma ◽  
Suman Yadav ◽  
Ankita Randev
Keyword(s):  
Body Weight ◽  
Granulosa Cells ◽  
Reproductive Toxicity ◽  
Concomitant Administration ◽  
Exposure Period ◽  
Treated Group ◽  
Female Rats ◽  
Corpora Lutea ◽  
Combined Administration ◽  
Increasing Trends

Both aluminium and ethanol are pro-oxidants and toxic. Uncontrolled use of aluminium and increasing trends of ethanol consumption in India increased the chance of coexposure to aluminium and ethanol. There are possibilities, that both of them follow common mechanisms to produce reproductive toxicity. The present study was planned to identify the effects of aluminium administration on the microscopic structure of ovary and to clarify any possible protection conferred by the concomitant administration of ethanol. Sixteen female rats divided into one control and three experimental groups exposed to aluminium (4.2mg/kg body weight) and ethanol (1gm/kg body weight) for 3 months. After the exposure period, ovaries were processed for light microscopic examination. Ovary showed significant atretic follicles with degenerated ova and vacuolation. Rupture of zona pellucida in oocyte seen in aluminium treated animals. Ethanol treated group showing absence of growing follicles, increased large corpora lutea. Dilated and congested vessels were observed in the growing follicle. The effects of combined administration of aluminium and ethanol treated groups showed with acute degeneration of growing follicles, with desquamation of pyknotic granulosa cells and degenerated oocyte. Multiple vacuoles of degenerated granulosa cells with dilated congested vessels and edema seen. Hyaline material seen inside the degenerating follicles. It has been suggested that the ethanol induced augmentation of impacts of aluminium on the Ovary.

scholarly journals effect of ascorbic acid as a feed additive on indicators of the reproductive performance of Pulawska breed gilts

2016 ◽  
Vol 72 (6) ◽  
pp. 378-382
Author(s):  
Jerzy Lechowski ◽  
Anna Kasprzyk ◽  
Mirosław Tyra ◽  
Beata Trawińska
Keyword(s):  
Body Weight ◽  
Vitamin C ◽  
Reproductive Performance ◽  
Reproductive Organs ◽  
The Body ◽  
Reproductive Value ◽  
Feed Additive ◽  
Corpora Lutea ◽  
Reproduction Cycle ◽  
Experimental Group

The aim of the study was to determine the effect of vitamin C on indicators of gilts and sows reproductive value. The study was conducted using 64 gilts of the Pulawska breed. The experimental gilts received a supplement of vitamin C in an amount of 2.4 g daily throughout the whole experiment. Sixteen gilts from both control and experimental groups were mated in their third estrus. In order to determine the number of corpora lutea and the state of development of the reproductive organs, other gilts from the control and experimental groups were slaughtered after third estrus between the 5th and 10th day of the following estrous cycle. The results showed that gilts from the experimental group were characterized by higher potential fertility, as well as reared more piglets per litter. In the body weight and backfat thickness of sows during the first reproduction cycle in the control and experimental groups were not statistically significantly different. The piglets in the experimental group had higher body weight in the 1st and 21st day of rearing. Larger contents of protein, immunoglobulins, and vitamin C were recorded in the colostrum and milk from experimental sows.

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