scholarly journals A new concept in minimally invasive embryo transfer

2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Jarosław Wieczorek ◽  
Ewa Stodolak-Zych ◽  
Krzysztof Okoń ◽  
Jurij Kosejuk ◽  
Magdalena Bryła ◽  
...  
Keyword(s):  
Embryo Transfer ◽  
Material Properties ◽  
Histopathological Examination ◽  
Second Stage ◽  
Embryo Cultures ◽  
Low Efficiency ◽  
Two Stages ◽  
New Generation

AbstractConsiderable variation in embryo transfer (ET) catheter types, diverging opinions on their quality and functionality, complications following the insertion of catheters, low efficiency of the application of ET methods in humans, and their widely varying efficiency in animals demonstrate the need to improve ET methods and to look for new types of catheters. Such an opportunity is offered by the introduction of catheters made of new-generation biomaterials. This study was aimed to introduce a new generation of biomaterials into reproductive biotechnology. New-generation materials were compared with materials that have been used for many years, and the functionality of newly produced catheters was compared in vivo. Five types of biomaterials were tested: polycaprolactone (PCL), dibutyryl chitin (DBC), polypropylene (PP), polyethylene (PE) and polylactide (PLA). The study was carried out in two stages. Firstly, the basic utility parameters such as geometric stability, surface structure and catheter resistance were evaluated. Subsequently, the biocompatibility of selected biomaterials in embryo cultures was examined, and the development potential of the obtained blastocysts was evaluated. In the second stage, in in vivo with live animals, the biomaterials were tested for biocompatibility and the obtained catheters were examined for their ET functionality. Efficiency with the use of the newly produced catheters was determined, the quality of the blastocysts obtained after embryo culture in the uterus was assessed, and oviducts were subjected to histopathological examination after embryo transfer. Of the tested biomaterials, only polyethylene (PE) showed adequate biological and material properties and proved suitable for production of ET catheters.

LATTICE SIMULATION OF NASCENT PEPTIDE FOLDING

2004 ◽  
Vol 18 (15) ◽  
pp. 2195-2202 ◽  
Author(s):  
JIAFENG ZHUO ◽  
LINSEN ZHANG ◽  
CHANGJUN CHEN ◽  
YI HE ◽  
YI XIAO
Keyword(s):  
Lattice Model ◽  
Peptide Folding ◽  
Synthesis Process ◽  
Lattice Simulation ◽  
Native State ◽  
Second Stage ◽  
Nascent Peptide ◽  
Two Stages

The nascent peptide folding in vivo is different from the denatured peptide refolding in vitro and can be divided into two stages. In the first stage, the peptide is folding as it is being synthesized until the whole peptide chain is synthesized. The final conformation formed in this stage is called as nascent state. In the second stage, the protein folds beginning with the nascent state formed in the first stage into the native state. We use a lattice model to simulate these two stages and investigate the folding time of the nascent peptide comparing with that of the denatured peptide refolding. Our results show that the synthesis process may affect the folding time of the nascent peptide. This may be helpful to understand why the former folds faster than the latter.

A novel two-stage method of plant seedlings classification based on deep learning

2021 ◽  
pp. 1-11
Author(s):  
Tianhong Dai ◽  
Shijie Cong ◽  
Jianping Huang ◽  
Yanwen Zhang ◽  
Xinwang Huang ◽  
...  
Keyword(s):  
Deep Learning ◽  
Learning Technology ◽  
Two Stage ◽  
Second Stage ◽  
Stage Classification ◽  
Different Types ◽  
Two Stages ◽  
Plant Seedlings

In agricultural production, weed removal is an important part of crop cultivation, but inevitably, other plants compete with crops for nutrients. Only by identifying and removing weeds can the quality of the harvest be guaranteed. Therefore, the distinction between weeds and crops is particularly important. Recently, deep learning technology has also been applied to the field of botany, and achieved good results. Convolutional neural networks are widely used in deep learning because of their excellent classification effects. The purpose of this article is to find a new method of plant seedling classification. This method includes two stages: image segmentation and image classification. The first stage is to use the improved U-Net to segment the dataset, and the second stage is to use six classification networks to classify the seedlings of the segmented dataset. The dataset used for the experiment contained 12 different types of plants, namely, 3 crops and 9 weeds. The model was evaluated by the multi-class statistical analysis of accuracy, recall, precision, and F1-score. The results show that the two-stage classification method combining the improved U-Net segmentation network and the classification network was more conducive to the classification of plant seedlings, and the classification accuracy reaches 97.7%.

HEMATOPOETIC STEM CELL RESPONSE TO STIMULATION OF EXTRACTS FROM ANIMAL MATERIALS

2016 ◽  
Vol 78 (5-6) ◽  
Author(s):  
Ivan Smirnov ◽  
Victor Keino ◽  
Ksenia Goryacheva ◽  
Alexander Shunk ◽  
Alexander Bondarev ◽  
...  
Keyword(s):  
Raw Materials ◽  
Raw Material ◽  
Animal Origin ◽  
Aqueous Extracts ◽  
Second Stage ◽  
Hematopoetic Stem Cell ◽  
Two Stages ◽  
Stimulation Of

The article presents the results of the research hemostimulating activity of aqueous extracts of antler young Siberean stag and drone larvae homogenate. These substrates were obtained from raw materials of animal origin. Altai Krai andAltaiRepublicare subjects of theRussian Federationwhich is the place of production of the raw material. Experiments were conducted in two stages. The first stage - in vitro, which included a research of experimental substrates on the culture of mouse marrow cells. During the experiments were obtained different results. We counted the number of colonies grown in cell culture for this. The second stage of experimenters - in vivo. It included an assessment of the myeloprotector on model of cytostatic myelosuppression of mice and analysis of bone marrow and peripheral blood.

Effect of Hygrothermal Transfer on Multilayer Walls Behavior, Assessment of Condensation Risk

2014 ◽  
Vol 1051 ◽  
pp. 647-655 ◽  
Author(s):  
Mohammed Yacine Ferroukhi ◽  
Rafik Belarbi ◽  
Karim Limam
Keyword(s):  
Material Properties ◽  
Moisture Transfer ◽  
Transfer Model ◽  
Construction Sector ◽  
Moisture Capacity ◽  
Second Stage ◽  
Hygrothermal Behavior ◽  
Multilayer Wall ◽  
Simulation Results ◽  
Two Stages

Good insulation of envelopes in new or rehabilitated buildings represents one of the major issues in reducing the energy consumption in the construction sector. In order to ensure better insulation, a material compatible with the wall must be chosen to guarantees both good thermal insulation and reduction of humidity risk.This study reviews the risks related to moisture, particularly those related to condensation one occurring in buildings. Two stages were conducted during the study. Firstly, a coupled heat, air and moisture transfer model in a multilayer wall was developed. This model took into account both the discontinuity at the interfaces between the wall layers and the evolution of material properties depending on the water content. Then, applications to assess hygrothermal behavior of multilayer walls and predict of condensation risk into them were examined.Secondly, a parametric study was achieved on hygrothermal properties of insulation materials. The objective of this second stage was to evaluate sensitivity magnitude of different material properties on hygrothermal behavior of multilayers walls.This study focused on the most influential thermal and hydric properties in hygrothermal transfer model. Thus, thermal conductivity for heat transfer, total moisture permeability and moisture capacity to hydric transfer were selected.Finally, simulation results were analyzed and conclusions were derived on the effect of key parameters on the hygrothermal behavior in the walls and the conditions causing condensation risk to increase.

scholarly journals Madrasah Principal Leadership Role in Improving Teachers’ Performance

2021 ◽  
Vol 1 (1) ◽  
pp. 10
Author(s):  
Sarmi Sinta Putri ◽  
Safri Mardison
Keyword(s):  
Principal Leadership ◽  
Program Planning ◽  
Preliminary Observation ◽  
Leadership Role ◽  
Data Display ◽  
Learning Program ◽  
Second Stage ◽  
Two Stages

This study aims to describe the role of the madrasah principal in improving teachers’ performance. This was a descriptive qualitative study. Observation, interviews, and documentation were used to collect the data. The source of data were the principal and teachers of MTsN 4 Muko-muko. Data were analyzed in two stages: the first stage was preliminary observation and the second stage was the research which included three steps, namely data reduction, data display, and data verification. Trustworthiness data were tested by conducting triangulation. The result indicated that principal leadership in improving the quality of teachers’ performance included Discipline Development Supervision Program (PPD), Supervision and Evaluation (SDE) activity, and Learning Program Planning. These findings are the basic of how discipline, supervision, and learning program planning become the indicators of guidance to improve the quality of teachers’ performance.    

scholarly journals PENGGUNAAN KARTU KUARTET UNTUK MENINGKATKAN PEMAHAMAN KEBERAGAMAN SENI TRADISI NUSANTARA PADA SISWA SEKOLAH DASAR

2014 ◽  
Vol 1 (1) ◽  
Author(s):  
Karsono ◽  
Yudianto Sujana ◽  
Joko Daryanto ◽  
Ngadino Yustinus
Keyword(s):  
Elementary School ◽  
Learning Process ◽  
Research Approach ◽  
Traditional Art ◽  
Is Implementation ◽  
Second Stage ◽  
Action Research Approach ◽  
Two Stages ◽  
Traditional Arts

<p>The purpose of this research is to improve the quality of the learning process subjects Culture Art and Skills in elementary school, especially in understanding of the diversity of Indonesian traditional art. This research was conducted in two stages. First, to develop a media card quartet, and second stage is implementation of the themed quartet card into the learning process in the classroom. Research methods use classroom action research approach because the quartet card media was subsequently used in the learning process in the classroom to improve understanding. Source of data collected by conducting interviews, tests, observations, and documentation. The analysis of the data using Miles and Hubberman’s interactive model, includes the step of data reduction, data display, and conclusion. The results showed that the use of card-themed quartet can enhance students' understanding of elementary school SDN 01 Jatisawit and SDN Dilem in the diversity of Indonesian traditional art material. Keywords: instructional media, cards quartet, the diversity of traditional arts.</p><p>Penelitian ini hendak memperbaiki kualitas proses pembelajaran mata pelajaran Seni Budaya dan Keterampilan (SBK) di sekolah dasar (SD) dalam aspek pemahaman peserta didik akan materi pembelajaran keragaman seni tradisi nusantara. Secara garis besar penelitian ini dilakukan dalam dua tahapan. Tahapan pertama melakukan pengembangan media kartu kuartet, dan tahapan kedua yaitu tahap implementasi kartu kuartet bertema budaya nusantara tersebut ke dalam proses pembelajaran di kelas. Metode penelitian menggunakan pendekatan penelitian tindakan kelas karena media kartu kuartet yang dibuat selanjutnya digunakan dalam proses pembelajaran di kelas untuk meningkatkan pemahaman materi keragaman seni tradisi. Sumber data dikumpulkan dengan melakukan wawancara, tes, observasi, dan dokumentasi. Sedangkan analisis data menggunakan model analisis interaktif dari Miles dan Huberman meliputi tahap reduksi data, penyajian data, dan penarikan kesimpulan. Hasil penelitian<br />memperlihatkan bahwa penggunaan kartu kuartet bertema seni tardisi nusantara dapat meningkatkan pemahaman siswa sekolah dasar SDN 01 Jatisawit dan SDN Dilem, dalam materi keberagaman seni tradisi nusantara.</p>

Rubberised fabric for fuel soft storage tanks*

2018 ◽  
Vol 45 (7) ◽  
pp. 322-324
Author(s):  
Yu.N. Rybakov ◽  
A.V. Dedov ◽  
D.S. Plokhoi ◽  
D.V. Kolotilin
Keyword(s):  
Diffusion Processes ◽  
Motor Fuel ◽  
Residual Monomer ◽  
Experimental Conditions ◽  
Second Stage ◽  
Before And After ◽  
Nitrile Rubbers ◽  
Two Stages ◽  
Kinetics Of

The kinetics of extraction of dibutyl sebacinate by different grades of motor fuel from rubberised fabric based on a blend of nitrile rubbers SKN-26M and SKN-40M was investigated. The kinetics was determined according to the GOST 9.030-74 standard from the change in weight of the specimen after holding in fuel at 50°C and drying. The change in quality of the fuel after contact with the fabric was recorded from the change in the content of existent gum in fuel according to GOST 1567-97 (ISO 6246-95) and its acidity according to GOST 5985-79 before and after contact with the rubberised fabric. It was shown that the process of extraction proceeds in two stages, the time of the first of which under experimental conditions is not dependent on the type of fuel. The rate of the first stage is much higher than the rate of the second stage. The relation of rates and proportion of extracted fuel at the first and second stages depends on the type of fuel. It can be asserted that the surplus amount of extracted substances is made up of impurities, which include residual monomer or its derivatives and substances used in the synthesis of the rubber. The mechanism of extraction with extractant counterflow into the vulcanisate and its dependence on the grade of petrol is proposed, based on allowance for the diffusion processes in the system.

Do Teacher Attitudes Impact TeachingThrough Information Technology

2017 ◽  
Vol 4 (24) ◽  
Author(s):  
Suman Kumari Katoch
Keyword(s):  
Information Technology ◽  
Study Data ◽  
Survey Method ◽  
School Teachers ◽  
Second Stage ◽  
Good Sample ◽  
Two Stages ◽  
Quality Of Research ◽  
Selection Of

Teachers’ styles, and mainly their attitudes, are strong context outcomes, rooted in experience and do not become automatic routine conducts, in the sense that they are developed via very slow interactions and become well established constructs for each individual only after some time. In that sense attitudes can be modified only by each individual, when he/she becomes aware, via elements and evidence, that new postures would be better to deal with the world around. In the present study data regarding the attitude of teachers towards information technology was gathered with the help of survey method. All the school teachers of districts Bilaspur and Hamirpur of Himachal Pradesh constituted the population of the study. In order to ensure high quality of research, selection of a good sample is must. For this purpose sampling was done at two stages. At the first stage 14 schools were selected and in the second stage 150 teachers were selected randomly from the each selected school. Keeping in view the nature of the present study the investigator used the standardized tool, “Attitude towards Information Technology Scales. The reliability of the tool is 0.88. To find out the significance of difference between the various groups ‘t’-test was applied. The findings of the study revealed that gender-wise, locality-wise, school teachers do not differed significantlyin their attitude towards information technology.

scholarly journals In vivo self-assembled small RNAs as a new generation of RNAi therapeutics

Cell Research ◽  
2021 ◽  
Author(s):  
Zheng Fu ◽  
Xiang Zhang ◽  
Xinyan Zhou ◽  
Uzair Ur-Rehman ◽  
Mengchao Yu ◽  
...  
Keyword(s):  
Self Assembly ◽  
Direct Injection ◽  
Sirna Delivery ◽  
Genetic Circuit ◽  
Genetic Circuits ◽  
Rnai Therapeutics ◽  
Two Stages ◽  
New Generation ◽  
Rnai Therapy

AbstractRNAi therapy has undergone two stages of development, direct injection of synthetic siRNAs and delivery with artificial vehicles or conjugated ligands; both have not solved the problem of efficient in vivo siRNA delivery. Here, we present a proof-of-principle strategy that reprogrammes host liver with genetic circuits to direct the synthesis and self-assembly of siRNAs into secretory exosomes and facilitate the in vivo delivery of siRNAs through circulating exosomes. By combination of different genetic circuit modules, in vivo assembled siRNAs are systematically distributed to multiple tissues or targeted to specific tissues (e.g., brain), inducing potent target gene silencing in these tissues. The therapeutic value of our strategy is demonstrated by programmed silencing of critical targets associated with various diseases, including EGFR/KRAS in lung cancer, EGFR/TNC in glioblastoma and PTP1B in obesity. Overall, our strategy represents a next generation RNAi therapeutics, which makes RNAi therapy feasible.

scholarly journals In Vivo Evaluation of Biocompatibility of Three Biomaterials Used in Endodontics for Prosthetic Purposes in Complex Rehabilitation Treatment

2021 ◽  
Vol 11 (14) ◽  
pp. 6519
Author(s):  
Liana Aminov ◽  
Eusebiu Viorel Sindilar ◽  
Aurelian Sorin Pasca ◽  
Cristina Antohi ◽  
Yllka Decolli ◽  
...  
Keyword(s):  
Histopathological Examination ◽  
Significant Proportion ◽  
Mineral Trioxide Aggregate ◽  
In Vivo Evaluation ◽  
Oral Rehabilitation ◽  
Prosthetic Devices ◽  
Stomatognathic System ◽  
The Ideal

The ideal biomaterial used in endodontics in the process of sealing the radicular canals should possess a group of qualities for a predictable outcome: biocompatibility, initiation of ontogenesis and cementogenesis, ease of handling, sufficient manipulation time, and convenient price. For a perfect sealing, the root canal treatment can be followed by prosthetic restoration. This study of biocompatibility aims to determine the quantification of the local reaction following the implantation of three biomaterials in the rabbit subcutaneous connective tissue. The used biomaterials with particular reparative properties are: MTA (Mineral Trioxide Aggregate, Dentsply, Tulsa Dental, Johnson City, TN, USA), Sealapex (Kerr, Switzerland), and DiaRoot BioAggregate (Innovative BioCaramix Inc, Vancouver, BC, Canada). The first two biomaterials (MTA, Sealapex) are already being used in endodontic treatments, and the latter was newly introduced during the concrete development of the study. This is an experimental study focused on qualitative and quantitative analysis based on histopathological examination and underlined by the positive result of the study undertaken of the applicability of oral rehabilitation treatments, increasing patients’ quality of life by a significant proportion of 95%, and generating optimal functionality of the stomatognathic system with prosthetic devices as well as accomplishing the objectives of homeostasis.

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