In vitro investigation of the antioxidant and antimicrobial effects of hydro-alcoholic and aqueous extracts of Globularia alypum L.

AbstractThe aim of this work was to establish the antioxidant and antimicrobial activity of hydro-alcoholic and aqueous extracts prepared from the leaves of Globularia alypum L. The quantitative estimation of total polyphenols (by the Folin-Ciocalteu method) and total flavonoids (by the method of aluminium trichloride) showed that the aqueous and ethyl acetate extracts had the highest content of phenolic and flavonoid compounds. Moreover, the results of antioxidant power assessed by both method DPPH and phosphomolybdenum indicated that leaves extracts of G. alypum expressed a considerable activity. The evaluation of the antimicrobial effects, using the disc diffusion method from antibacterial screening and the direct contact method from the antifungal activity, indicated strong antibacterial and antifungal activity.

Download Full-text

An In Vitro Study on the Antimicrobial Properties of Essential Oil Modified Resin Composite against Oral Pathogens

Materials ◽

10.3390/ma13194383 ◽

2020 ◽

Vol 13 (19) ◽

pp. 4383

Author(s):

Barbara Lapinska ◽

Aleksandra Szram ◽

Beata Zarzycka ◽

Janina Grzegorczyk ◽

Louis Hardan ◽

...

Keyword(s):

Antimicrobial Activity ◽

Antibacterial Activity ◽

Antifungal Activity ◽

Antimicrobial Agents ◽

Resin Composite ◽

Antimicrobial Properties ◽

In Vitro Study ◽

Diffusion Method ◽

Oral Pathogens

Modifying the composition of dental restorative materials with antimicrobial agents might induce their antibacterial potential against cariogenic bacteria, e.g., S.mutans and L.acidophilus, as well as antifungal effect on C.albicans that are major oral pathogens. Essential oils (EOs) are widely known for antimicrobial activity and are successfully used in dental industry. The study aimed at evaluating antibacterial and antifungal activity of EOs and composite resin material (CR) modified with EO against oral pathogens. Ten EOs (i.e., anise, cinnamon, citronella, clove, geranium, lavender, limette, mint, rosemary thyme) were tested using agar diffusion method. Cinnamon and thyme EOs showed significantly highest antibacterial activity against S.mutans and L.acidophilus among all tested EOs. Anise and limette EOs showed no antibacterial activity against S.mutans. All tested EOs exhibited antifungal activity against C.albicans, whereas cinnamon EO showed significantly highest and limette EO significantly lowest activity. Next, 1, 2 or 5 µL of cinnamon EO was introduced into 2 g of CR and microbiologically tested. The modified CR showed higher antimicrobial activity in comparison to unmodified one. CR containing 2 µL of EO showed the best antimicrobial properties against S.mutans and C.albicans, while CR modified with 1 µL of EO showed the best antimicrobial properties against L.acidophilus.

Download Full-text

Phytochemical Screening, Polyphenols Content, Antioxidant Power, and Antibacterial Activity of Herniaria hirsuta from Morocco

The Scientific World JOURNAL ◽

10.1155/2018/7470384 ◽

2018 ◽

Vol 2018 ◽

pp. 1-7 ◽

Cited By ~ 1

Author(s):

Kenza Ammor ◽

Dalila Bousta ◽

Sanae Jennan ◽

Bahia Bennani ◽

Abdellah Chaqroune ◽

...

Keyword(s):

Antibacterial Activity ◽

Antioxidant Activities ◽

Radical Scavenging ◽

Reducing Power ◽

Antibacterial Activities ◽

Diffusion Method ◽

Antioxidant Power ◽

Negative Effect ◽

Hydroethanolic Extract

The aim of this study is to investigate in vitro antioxidant and antibacterial activities of the aqueous and hydroethanolic extracts for aerial parts of Herniaria hirsuta. Extracts were screened for their possible antioxidant activities by three tests: DPPH free radical-scavenging, reducing power, and molybdenum systems. The screening of antibacterial activity of extracts was individually evaluated against sixteen bacteria species using a disc diffusion method. Flavonoids, total phenols, and tannins content were performed for both extracts. It shows higher content in the hydroethanolic extract. The hydroethanolic extract showed a significant antioxidant activity for the three methods studies to the aqueous extract, but nonsignificant results compared to the reference (BHT). However, both extracts have negative effect on the strains studies for the antibacterial activity.

Download Full-text

In Vitro Antimicrobial and Antioxidant Activities of Organic and Aqueous Extracts of Bacillus clausii KP10

Journal of the chemical society of pakistan ◽

10.52568/000721/jcsp/41.01.2019 ◽

2019 ◽

Vol 41 (1) ◽

pp. 161-161

Author(s):

Farzana Nighat and Zahid Mushtaq Farzana Nighat and Zahid Mushtaq

Keyword(s):

Hot Springs ◽

Antioxidant Activities ◽

Radical Scavenging ◽

Ethanolic Extract ◽

Antimicrobial Activities ◽

Diffusion Method ◽

Aqueous Extracts ◽

Current Case ◽

Bacillus Clausii

Humans and plants are experiencing different kinds of diseases. Many of these diseases are mainly caused by microorganisms like fungi, bacteria and viruses that adversely affect their hosts. Bioactive compounds produced by bacteria can be used to treat the infections caused by these microorganisms. Free radicals that can cause serious damage to the cell can be repressed by antioxidants that are produced by bacteria as secondary compounds. The current case study was therefore planned to investigate the antimicrobial and antioxidant potentials of the organic and aqueous extracts of Bacillus clausii KP10 isolated recently by our group from hot springs of Tatta Pani, Poonch, Azad Kashmir. The antimicrobial activities were assessed using well diffusion method against two Gram positive bacteria; B. subtilis, and S. aureus, and two Gram negative bacteria; E. coli and P. multocida and three fungal strains; A. niger, A. parasiticus and A. ustus. Antioxidant activities of the extracts were done by DPPH free radical scavenging assay. In our experiments the maximum zones of inhibition were shown by ethanolic extract against P. multocida (21 mm), S. aureus (13 mm) and methanol soluble fraction showed zone of inhibition against A. ustus (21 mm) as compared to the standard ciprofloxacin for bacteria and as antifungal agents with terbinafine. In our studies n-hexane fraction of B. clausii KP10 showed maximum 88.67% antioxidant activity. Finally, results were analyzed statistically through analysis of variance (ANOVA). Hence it is proved that the bioactive fractions obtained from B. clausii KP10 are good source of antimicrobials and antioxidants and they can be used as medicines in future for the respective treatments.

Download Full-text

IN VITRO ANTIMICROBIAL ACTIVITY OF CYNODON DACTYLON (L.) PERS. LEAF EXTRACTS. ANTIMICROBIAL ACTIVITY BY ZONE OF INHIBITION ESTIMATION

INDIAN DRUGS ◽

10.53879/id.52.04.10125 ◽

2015 ◽

Vol 52 (04) ◽

pp. 37-41

Author(s):

S. M Pawaskar ◽

◽

K. C. Sasangan

Keyword(s):

Antimicrobial Activity ◽

Antifungal Activity ◽

Ethyl Acetate ◽

Petroleum Ether ◽

Cynodon Dactylon ◽

Diffusion Method ◽

Zone Of Inhibition ◽

Leaf Extracts ◽

In Vitro Antimicrobial Activity

The present study was undertaken to evaluate in vitro antimicrobial activity of the successive leaf extracts of Cynodon dactylon in petroleum ether, ethyl acetate, acetone, ethanol, methanol and water, against various gram positive & gram negative bacterial strains using zone of inhibition. Both Agar well diffusion method & Agar disc diffusion method were used to evaluate the antibacterial efficacy. The microorganisms used in the test were – Escherichia coli, Proteus vulgaris, Staphylococcus aureus, Klebsiella pneumoniae, Pseudomonas aeruginosa, Shigella flexneri, Salmonella typhi, Salmonella paratyphi A, Salmonella paratyphi B, Bacillus subtilis, Streptococcus pyogenes, Vibrio cholerac and Enterobacter aerogenes. Two fungal strains - Candida albicans and Saccharomyces cerevisiae were also tested to evaluate the antifungal potential of the said plant extract. The reference antibiotics chloramphenicol & ampicillin (antibacterial); nystatin & clotrimazole and (antifungal) were also tested against these standard microorganisms used in the assay and the results were compared with that of the plant extracts.The results of the study revealed that all the seven successive extracts of the leaf powder of Cynodon dactylon ( L.) Pers. exhibited prominent antimicrobial and antifungal activity against all microorganisms used in the study. The nonpolar extracts i.e. petroleum ether, ethyl acetate and acetone showed the most significant antibacterial and antifungal activity against all tested organisms. The petroleum ether and ethyl acetate extracts showing maximum inhibition in the range of 8 mm - 15 mm. This was closely followed by, acetone extract, which showed the inhibition in the range of 9 mm - 13 mm.

Download Full-text

Antifungal Activity of the Essential Oil of Echinops kebericho Mesfin: An In Vitro Study

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2020/3101324 ◽

2020 ◽

Vol 2020 ◽

pp. 1-7

Author(s):

Tamirat Bekele Beressa ◽

Serawit Deyno ◽

Paul E. Alele

Keyword(s):

Candida Albicans ◽

Antifungal Activity ◽

Essential Oil ◽

Cryptococcus Neoformans ◽

Antioxidant Properties ◽

Pathogenic Fungi ◽

Diffusion Method ◽

Potential Candidate ◽

Standard Drug

Background. Echinops kebericho is an endemic medicinal plant in Ethiopia widely used in the treatment of infectious and noninfectious diseases. Essential oils are known for their antibacterial, antifungal, antiviral, insecticidal, and antioxidant properties. This study evaluated the antifungal activity of essential oil from E. kebericho against four common pathogenic fungi and two standard strains. Methods. The essential oil was obtained by hydrodistillation. The antifungal screening was done by agar well diffusion method. Minimal inhibitory concentrations (MICs) were determined by broth microdilution. Minimal fungicidal concentrations (MFCs) were determined by subculturing fungal strains with no visible growth onto a Sabouraud dextrose agar (SDA) plate. Results. Candida albicans and Cryptococcus neoformans were highly sensitive while Aspergillus flavus did not show sensitivity up to 1 mg/ml of essential oil; MICs ranged from 0.083 mg/ml to 0.208 mg/ml. Concentration and fungal species showed significant dose-dependent associations ( p < 0.0001 ) with antifungal activity. The MICs of essential oil were comparable to those of the standard drug (fluconazole) against C. glabrata and C. krusei. The lowest MFC of the essential oil was observed against Candida parapsilosis (0.145 mg/ml) while the highest MFC was against Candida krusei (0.667 mg/ml). Conclusion. Echinops kebericho essential oil showed noteworthy antifungal activity against Cryptococcus neoformans, Candida albicans, and Candida glabrata and could be a potential candidate for further antifungal drug development.

Download Full-text

In Vitro Antibacterial and Antifungal Activity and Datura Innoxia Extracts

International Journal of Environment ◽

10.3126/ije.v3i3.11077 ◽

2014 ◽

Vol 3 (3) ◽

pp. 173-185

Author(s):

Adam IY Shama ◽

YM Abd-Kreem ◽

AA Fadowa ◽

RM Samar ◽

MK Sabahelkhier

Keyword(s):

Antimicrobial Activity ◽

Plant Extracts ◽

Antifungal Drugs ◽

Diffusion Method ◽

Antibacterial Drug ◽

Aqueous Extracts ◽

Datura Innoxia ◽

Methanolic Extracts ◽

Leaves And Roots

The aim of this study was evaluated the Antimicrobial Activity of extraction of Datura innoxia (Seeds, leaves and roots). Datura innoxia Seeds, leaves and roots were collected to examine their antimicrobial activity. Extracts of different parts of the plant were tested against standard microorganisms by using the agar- well diffusion method. Extracts of methanol, and aqueous of seeds, leaves and roots were prepared and tested against four types of bacteria namely: Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa and Proteus vulgaris and two types of fungi namely: Aspergillus niger and Candida albicans. The methanolic and aqueous extracts of leaves showed high activities against fungi (A. niger) and less effect on the all bacteria. The methanolic extracts of seeds showed high activities against all organisms except fungi (C. albicanas), while the aqueous extracts of seeds showed no activity on the bacteria. All organisms were examined against known standard antibiotics and then compare the results of plant extracts with standard antibiotics. The results indicated that the antibacterial drug is less active than the plant extracts, while the antifungal drugs are more active than the plant extracts. DOI: http://dx.doi.org/10.3126/ije.v3i3.11077 International Journal of Environment Vol.3(3) 2014: 173-185

Download Full-text

Phenolic contents and in vitro investigation of the antioxidant, enzyme inhibitory, photoprotective, and antimicrobial effects of the organic extracts of Pelargonium graveolens growing in Morocco

Biocatalysis and Agricultural Biotechnology ◽

10.1016/j.bcab.2020.101819 ◽

2020 ◽

Vol 29 ◽

pp. 101819

Author(s):

Soukaina El Aanachi ◽

Lynda Gali ◽

Salah Neghmouche Nacer ◽

Chawki Bensouici ◽

Khadija Dari ◽

...

Keyword(s):

Antioxidant Enzyme ◽

Pelargonium Graveolens ◽

Antimicrobial Effects ◽

Phenolic Contents ◽

In Vitro Investigation ◽

Organic Extracts

Download Full-text

Microwave irradiation enhances the in vitro antifungal activity of citrus by-product aqueous extracts against Alternaria alternata

International Journal of Food Science & Technology ◽

10.1111/ijfs.13732 ◽

2018 ◽

Vol 53 (6) ◽

pp. 1510-1517 ◽

Cited By ~ 4

Author(s):

Konstantinos Papoutsis ◽

Quan V. Vuong ◽

Len Tesoriero ◽

Penta Pristijono ◽

Costas E. Stathopoulos ◽

...

Keyword(s):

Antifungal Activity ◽

Microwave Irradiation ◽

Alternaria Alternata ◽

Aqueous Extracts ◽

In Vitro Antifungal Activity

Download Full-text

Evaluation of Antibacterial Activity of Combined Plant Extract of Pyracantha crenulata and Zanthoxylum armatum

Universities' Journal of Phytochemistry and Ayurvedic Heights ◽

10.51129/ujpah-2020-29-2(5) ◽

2020 ◽

Vol 2 (29) ◽

pp. 32-36

Author(s):

Semwal Amit Negi Sweta

Keyword(s):

Antimicrobial Activity ◽

Antibacterial Activity ◽

Ethanolic Extract ◽

Diffusion Method ◽

Moderate Activity ◽

Aqueous Extracts ◽

Disk Diffusion Method ◽

Zanthoxylum Armatum ◽

Plant Extraction

Abstract-Medicinal plants represent an essential source of drugs and have played an important role in healthcaresystem.PyracanthacrenulataandZanthoxylum armatumhave been used as traditional medicine. The main aim of the study was to find the antibacterial activity of the selected plants against bacterialspecies:E.coliandPseudomonasaeruginosa. The solvents used for plant extraction were hexane, chloroform, ethanol and aqueous. The in vitro antimicrobial activity was performed by Agar disk diffusion method. The hexane and aqueous extracts showed moderate activity whereas theethanolicextractsshowedasignificantantibacterial activity. In the study Tetracycline was used as standard. The combined ethanolic extract of both the selected plant showed the synergistic effect on the bacterial strain tested. This leads to the conclusion that the combined effect can have possible application in the development of products as antimicrobial.

Download Full-text