scholarly journals Screening for Bio-flocculant Producing Bacterial Strains from Asa River in Ilorin Kwara State

2019 ◽  
Vol 4 (2) ◽  
pp. 59-67
Author(s):  
O.M. Kolawole ◽  
T.D. Yahaya ◽  
A.R. Lawal ◽  
O.A. Okunade ◽  
O.O. Famuwagun ◽  
...  

AbstractSynthetic flocculants in water treatment have been reported to be detrimental to both human health and the environment. Thus, there is a constant search for bio-flocculants that is safe and addresses the effects of synthetic polymers. This study was aimed at isolating bacteria with bio-flocculating potential, their molecular identification and phylogenetic relatedness, and the optimization of their flocculating abilities from an open water (Asa River) in Ilorin Kwara State. The effect of carbon source (glucose, lactose and starch), cations (FeSO4, KCl and CaCl2) and pH (2 to 12) was evaluated on the bio-flocculating activities of the isolates using kaolin clay. The seven (7) novel (new strains) isolates with their respective accession number from National Center for Biotechnology Information (NCBI) after molecular confirmation are Pseudomonas otitidis MTK01 (MK263227), Aeromonas caviea MTK02 (MK263228), Providencia alcalifaciens MTK03 (MK263229), Providencia sp. MTK05 (MK263230), Alcaligenes sp. MTK06 (MK263231), Klebsiella pneumoniae MTK07 (MK263232) and Klebsiella sp. MTK08 (MK263233) while Raoultella ornithinolytica MTK04 was also identified. Phylogenetic tree of relatedness showed close kin of the isolates to established bacteria sequence deposited at the NCBI GenBank. Although the flocculating rate of each isolates varied with different parameters that was used in the study, glucose was the most supportive followed by lactose and starch, CaCl2 was most supportive cation followed by KCl and FeSO4 while pH 12, 6, 8, 10, 2 and 4 were the order of decreasing flocculating rate of the medium. This study has reported the presence of eight (8) bio-flocculating bacteria (out of which 7 are new strains of bacteria) in an open water which has been further optimized for effective flocculating rate and thus provides an ecofriendly and harmless flocculants source that can be employed in water treatment procedures.

2020 ◽  
Vol 1 (1) ◽  
pp. 51-79
Author(s):  
Ludovic Llanes ◽  
Pascal Dubessay ◽  
Guillaume Pierre ◽  
Cédric Delattre ◽  
Philippe Michaud

In the last decades, many studies have been conducted on new materials to meet a growing industrial demand and to move scientific research forward. Superabsorbents are good examples of materials that have generated special attention in many fields for their ability to absorb and retain water up to 1000 times of their dry weight. They found many applications in hygiene products and other products, for a fast growing market of USD 9.58 Billion in 2019. Most of them are composed of synthetic polymers, which are often not environmentally friendly. Therefore, natural superabsorbents and particularly those based on polysaccharides have received a recent increased interest for their biodegradability, biocompatibility, and renewability. This review focuses on polysaccharide-based superabsorbents, on their properties, synthesis methods, and characterization. Their potential applications in many fields, such as biomedical and hygiene, agriculture, water treatment, and the building sector, are also reported with an interest in products already marketed.


Author(s):  
K. Shivalkar Yadav ◽  
D. B. Puranik ◽  
Mohamed Nadeem Fairoze ◽  
R. Prabha

Aims: The synthetic polymer plastics have become an integral part of contemporary life. Excess use of plastics and indiscriminate dumping of it in soil and water is polluting the environment. In order to overcome this problem, the production and applications of eco-friendly biodegradable products from microbes are becoming inevitable from the last decade and also are the good alternatives for synthetic polymers. Methods and Results: Polyhydroxyalkanoate producing bacterial strains were confirmed by serial dilution of sewage samples from dairies and pour plating using modified nutrient agar medium with 2% glucose and 0.3% sudan black. Commercial dairy sewage sample from III Dairy showed highest count of PHA producers (3.80 log10cfu/ml) followed by II Dairy (3.68 log10cfu/ml) and I Dairy (3.35 log10cfu/ml). On an average, 70 per cent were PHA producers among TBC of sewage samples. Conclusion: Dairy sewage sample from III Dairy showed highest count of PHA producers (3.80log10cfu/ml) Significance and Impact of the Study: This study provides importance of polyhydroxyalkanoates and their role against synthetic plastic by enumerating the polyhydroxyalkanoate (PHA) producing bacteria from Dairy sewage samples that can be effectively utilized for the synthesis of bioplastics.


2019 ◽  
Vol 1 (1) ◽  
Author(s):  
Anannya Banerjee ◽  
Sudipta Tripathi

Leather industries that uses the conventional chrome tanning process are subjected to high risk of contamination due to the emission of toxic Cr(VI) that poses a serious threat to the environment and human’s wellbeing. The present study were made to isolate and characterize chromium tolerant bacteria in the samples collected from four different plots of Bantala Tannery, Kolkata, West Bengal, India. Pure chromium tolerant bacterial strains were isolated from the tannery sludge samples and their relative MIC (Minimum Inhibitory Concentration) were recorded at different concentrations of Cr (VI) salts to select the highest chromium tolerant bacterium. The selected bacterium was further taken for their growth studies followed by different cultural, morphological and molecular analysis (16S rDNA). The bacterial strain was further studied through SEM (Scanning Electron microscopy) and EDX (Energy Dispersive X-ray) spectroscopy which revealed that TW4 was a gram positive, rod shaped, endospore forming, pleomorphic bacterium with phylogenetic similarities with Isoptericola sp. and genebank accession number SUB1732465 TW4 KX640927.


2021 ◽  
pp. 108-110
Author(s):  
Andriy Tevyashev ◽  
Olga Matvienko ◽  
Gleb Nikitenko

Water supply of large cities is carried out, as a rule, from open reservoirs - lakes, reservoirs, large rivers, ie from sources with significant water reserves. In this case, the water supply scheme includes: pumping stations of the first rise, which take water directly from the water; water treatment system (purification); pumping stations of the second and third rise, pumping clean water over long distances and supplying it to settlements [1]. If the settlement is located in an area where there are no large open water sources, or the geographical location of the settlement allows the use of groundwater, in which case artesian wells are used as a source of water supply. Usually small settlements are supplied with water from artesian wells. Artesian wells are widely used for water supply of villages, industrial water supply, for irrigation (irrigation systems), in the mining industry, to reduce the level of groundwater. The advantage of using artesian wells to supply the population with drinking water is that the water is extracted from great depths and purified using natural filters [2]. Thus, the cost of water treatment is significantly reduced. The disadvantage of using artesian wells is usually low productivity of the well, the strong dependence of water volumes in the well on climatic and weather conditions, depletion of the well as a result of large-scale construction or extraction of natural minerals such as shale gas [3].


2018 ◽  
Vol 19 (5) ◽  
pp. 1305-1312
Author(s):  
Chen Wang ◽  
Qi Zheng ◽  
Xiaoqiu Yang

Abstract Acesulfame (ACE) and sucralose (SUC) are ideal sewage markers for drinking water. In this paper, 33 samples were collected from eight cities' drinking water treatment plants (DWTPs) along the Yangtze River to analyse the occurrence and removal of ACE and SUC. These two compounds were determined using ultra-high performance liquid chromatography–high resolution mass spectrometry. The limits of detection for ACE and SUC were 1.75 ng/L and 0.03 ng/L, respectively. The results showed that ACE and SUC existed widely in DWTPs along the Yangtze River. The concentration of ACE was 13–320 ng/L and the concentration of SUC was 200–1,592 ng/L. In general, the concentrations of ACE and SUC increased from upstream to downstream but decreased after water treatment procedures at every DWTP. In most DWTPs, a 9–30% removal of ACE and SUC was achieved using traditional chlorination disinfection technology. Ozonation combined with granular activated carbon filtration techniques had a high removal efficiency (63.8% for ACE and 50.2% for SUC).


2018 ◽  
Vol 2018 ◽  
pp. 1-7 ◽  
Author(s):  
Bushra Uzair ◽  
Rehana Kausar ◽  
Syeda Asma Bano ◽  
Sammer Fatima ◽  
Malik Badshah ◽  
...  

The use of microbial technologies in agriculture is currently expanding quite rapidly with the identification of new bacterial strains, which are more effective in promoting plant growth. In the present study 18 strains of Pseudomonas were isolated from soil sample of Balochistan coastline. Among isolated Pseudomonas strains four designated as SP19, SP22, PS24, and SP25 exhibited biocontrol activities against phytopathogenic fungi, that is, Rhizopus microsporus, Fusarium oxysporum, Aspergillus niger, Alternaria alternata, and Penicillium digitatum; PS24 identified as Pseudomonas aeruginosa by 16srRNA gene bank accession number EU081518 was selected on the basis of its antifungal activity to explore its potential as plant growth promotion. PS24 showed multiple plant growth promoting attributes such as phosphate solubilization activity, indole acetic acid (IAA), siderophore, and HCN production. In order to determine the basis for antifungal properties, antibiotics were extracted from King B broth of PS24 and analyzed by TLC. Pyrrolnitrin antibiotic was detected in the culture of strain PS24. PS24 exhibited antifungal activities found to be positive for hydrogen cyanide synthase Hcn BC gene. Sequencing of gene of Hcn BC gene of strain PS24 revealed 99% homology with the Pseudomonas aeruginosa strain PA01. The sequence of PS24 had been submitted in gene bank accession number KR605499. Ps. aeruginosa PS24 with its multifunctional biocontrol possessions can be used to bioprotect the crop plants from phytopathogens.


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