Isolation and Characterization of Bacterium isolated from Bantala Tannery Solid Wastes

Leather industries that uses the conventional chrome tanning process are subjected to high risk of contamination due to the emission of toxic Cr(VI) that poses a serious threat to the environment and human’s wellbeing. The present study were made to isolate and characterize chromium tolerant bacteria in the samples collected from four different plots of Bantala Tannery, Kolkata, West Bengal, India. Pure chromium tolerant bacterial strains were isolated from the tannery sludge samples and their relative MIC (Minimum Inhibitory Concentration) were recorded at different concentrations of Cr (VI) salts to select the highest chromium tolerant bacterium. The selected bacterium was further taken for their growth studies followed by different cultural, morphological and molecular analysis (16S rDNA). The bacterial strain was further studied through SEM (Scanning Electron microscopy) and EDX (Energy Dispersive X-ray) spectroscopy which revealed that TW4 was a gram positive, rod shaped, endospore forming, pleomorphic bacterium with phylogenetic similarities with Isoptericola sp. and genebank accession number SUB1732465 TW4 KX640927.

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Isolation and Characterization of Arsenite Tolerant Bacterial Strains from Contaminated Water of West Bengal, India

International Journal of Scientific Research in Environmental Sciences ◽

10.12983/ijsres-2016-p0001-0011 ◽

2016 ◽

Vol 4 (1) ◽

pp. 1-11

Author(s):

Sabyasachi Chatterjee ◽

Yogesh Bhai Patel ◽

Sajjan Rajpoot ◽

Sonam Rani ◽

Naba Kumar Mondal

Keyword(s):

West Bengal ◽

Contaminated Water ◽

Bacterial Strains ◽

Isolation And Characterization

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Screening a Strain of Klebsiella sp. O852 and the Optimization of Fermentation Conditions for Trans-Dihydrocarvone Production

Molecules ◽

10.3390/molecules26092432 ◽

2021 ◽

Vol 26 (9) ◽

pp. 2432

Author(s):

Li Chen ◽

Lu-Lu Zhang ◽

Jing-Nan Ren ◽

Xiao Li ◽

Gang Fan ◽

...

Keyword(s):

Ethanol Concentration ◽

Flavor Compounds ◽

Accession Number ◽

Reaction Parameters ◽

Isolation And Characterization ◽

Biotransformation Process ◽

Pharmaceutical Industries ◽

Final Ethanol Concentration ◽

Optimization Of Fermentation

Flavors and fragrances have high commercial value in the food, cosmetic, chemical and pharmaceutical industries. It is interesting to investigate the isolation and characterization of new microorganisms with the ability to produce flavor compounds. In this study, a new strain of Klebsiella sp. O852 (accession number CCTCC M2020509) was isolated from decayed navel orange (Citrus sinensis (L.) Osbeck), which was proved to be capable of converting limonene to trans-dihydrocarvone. Besides, the optimization of various reaction parameters to enhance the trans-dihydrocarvone production in shake flask was performed for Klebsiella sp. O852. The results showed that the yield of trans-dihydrocarvone reached up to 1 058 mg/L when Klebsiella sp. O852 was incubated using LB-M medium for 4 h at 36 °C and 150 rpm, and the biotransformation process was monitored for 36 h after adding 1680 mg/L limonene/ethanol (final ethanol concentration of 0.8% (v/v)). The content of trans-dihydrocarvone increased 16 times after optimization. This study provided a basis and reference for producing trans-dihydrocarvone by biotransformation.

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Isolation and Characterization of Salt-Tolerant Bacterial Strains in Salt-Affected Soils of Erzurum, Turkey

Geomicrobiology Journal ◽

10.1080/01490451.2014.962674 ◽

2015 ◽

Vol 32 (6) ◽

pp. 521-529 ◽

Cited By ~ 11

Author(s):

Furkan Orhan ◽

Medine Gulluce

Keyword(s):

Salt Tolerant ◽

Bacterial Strains ◽

Isolation And Characterization ◽

Salt Affected Soils

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Characterization of a Novel Fructosyltransferase from Lactobacillus reuteri That Synthesizes High-Molecular-Weight Inulin and Inulin Oligosaccharides

Applied and Environmental Microbiology ◽

10.1128/aem.68.9.4390-4398.2002 ◽

2002 ◽

Vol 68 (9) ◽

pp. 4390-4398 ◽

Cited By ~ 104

Author(s):

S. A. F. T. van Hijum ◽

G. H. van Geel-Schutten ◽

H. Rahaoui ◽

M. J. E. C. van der Maarel ◽

L. Dijkhuizen

Keyword(s):

Molecular Weight ◽

High Molecular Weight ◽

Lactobacillus Reuteri ◽

Bacterial Strains ◽

Potential Health ◽

Isolation And Characterization ◽

A Cell ◽

Encoding Gene ◽

First Time

ABSTRACT Fructosyltransferase (FTF) enzymes produce fructose polymers (fructans) from sucrose. Here, we report the isolation and characterization of an FTF-encoding gene from Lactobacillus reuteri strain 121. A C-terminally truncated version of the ftf gene was successfully expressed in Escherichia coli. When incubated with sucrose, the purified recombinant FTF enzyme produced large amounts of fructo-oligosaccharides (FOS) with β-(2→1)-linked fructosyl units, plus a high-molecular-weight fructan polymer (>107) with β-(2→1) linkages (an inulin). FOS, but not inulin, was found in supernatants of L. reuteri strain 121 cultures grown on medium containing sucrose. Bacterial inulin production has been reported for only Streptococcus mutans strains. FOS production has been reported for a few bacterial strains. This paper reports the first-time isolation and molecular characterization of (i) a Lactobacillus ftf gene, (ii) an inulosucrase associated with a generally regarded as safe bacterium, (iii) an FTF enzyme synthesizing both a high molecular weight inulin and FOS, and (iv) an FTF protein containing a cell wall-anchoring LPXTG motif. The biological relevance and potential health benefits of an inulosucrase associated with an L. reuteri strain remain to be established.

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Isolation and characterization of lignocellulosic fiber from jute bast by the organic solvent degumming system: an alkali-free method

Textile Research Journal ◽

10.1177/00405175211015496 ◽

2021 ◽

pp. 004051752110154

Author(s):

Zhihui Qin ◽

Shuyuan Zhao ◽

Liu Liu ◽

Zhaohe Shi ◽

Longdi Cheng ◽

...

Keyword(s):

National Standards ◽

X Ray Diffraction ◽

High Concentration ◽

X Ray ◽

Lignocellulosic Fibers ◽

Lignocellulosic Fiber ◽

Isolation And Characterization ◽

Alkali Consumption ◽

Scanning Electron

Degumming is the dominant method for insolating lignocellulosic fibers in textile applications. Traditional alkaline degumming (TAL), as a common method, requires a high-concentration alkali and has been a severe challenge to the environment. In the research reported here, the possibility of innovative jute degumming by organic solvents 1-2 propylene glycol and a combination of additive green oxygen (GO-OS) was studied. The results revealed that fibers could be extracted by this system (under condition of 0.9% GO-OS, 180°C, 120 min), and obtained fibers with higher breaking tenacity (7.1 cN/dtex), yield (65.7%), breaking elongation (2.87%) and residual gum (11.7%), which all meet the requirement of the relevant Chinese Textile National Standards. Notably, the required reaction time (120 min) of the GO-OS system was 180 min shorter than that of the TAL method. Furthermore, the modifications introduced by the degumming effect on physicochemical aspects were characterized and confirmed by Fourier transform infrared spectroscopy, scanning electron microscopy and X-ray diffraction. This study provides a promising degumming method for separating jute lignocellulose without acid and alkali consumption.

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Isolation and characterization of bacterial strains with a hydrolytic profile with potential use in bioconversion of agroindustial by-products and waste

Food Science and Technology ◽

10.1590/s0101-20612013005000038 ◽

2013 ◽

Vol 33 (2) ◽

pp. 295-303 ◽

Cited By ~ 18

Author(s):

Cintia Anabela Mazzucotelli ◽

Alejandra Graciela Ponce ◽

Catalina Elena Kotlar ◽

María del Rosario Moreira

Keyword(s):

Bacterial Strains ◽

Isolation And Characterization ◽

By Products ◽

Potential Use

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Isolation and Characterization of Potentially Probiotic Bacterial Strains from Mice: Proof of Concept for Personalized Probiotics

Nutrients ◽

10.3390/nu10111684 ◽

2018 ◽

Vol 10 (11) ◽

pp. 1684 ◽

Cited By ~ 2

Author(s):

Larissa Celiberto ◽

Roseli Pinto ◽

Elizeu Rossi ◽

Bruce Vallance ◽

Daniela Cavallini

Keyword(s):

Intestinal Inflammation ◽

Activity Index ◽

Disease Activity Index ◽

Bacterial Strains ◽

Dss Colitis ◽

Isolation And Characterization ◽

Inflammatory Bowel ◽

Commercial Probiotics ◽

Lower Disease Activity

Modulation of the gut microbiota through the use of probiotics has been widely used to treat or prevent several intestinal diseases. However, inconsistent results have compromised the efficacy of this approach, especially in severe conditions such as inflammatory bowel disease (IBD). The purpose of our study was to develop a personalized probiotic strategy and assess its efficacy in a murine model of intestinal inflammation. Commensal bacterial strains were isolated from the feces of healthy mice and then administered back to the host as a personalized treatment in dextran sodium sulfate (DSS)-induced colitis. Colonic tissues were collected for histological analysis and to investigate inflammatory markers such as Il-1β, Il-6, TGF-β, and Il-10, and the enzyme myeloperoxidase as a neutrophil marker. The group that received the personalized probiotic showed reduced susceptibility to DSS-colitis as compared to a commercial probiotic. This protection was characterized by a lower disease activity index and reduced histopathological damage in the colon. Moreover, the personalized probiotic was more effective in modulating the host immune response, leading to decreased Il-1β and Il-6 and increased TGF-β and Il-10 expression. In conclusion, our study suggests that personalized probiotics may possess an advantage over commercial probiotics in treating dysbiotic-related conditions, possibly because they are derived directly from the host’s own microbiota.

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Isolation and characterization of a tetranuclear Pt–Fe⋯Fe–Pt intermediate en route to the trinuclear Pt–Fe–Pt cluster

Dalton Transactions ◽

10.1039/c7dt02583a ◽

2017 ◽

Vol 46 (40) ◽

pp. 14012-14020

Author(s):

Kazuhiro Uemura ◽

Masahiro Taoka

Keyword(s):

Single Crystal ◽

Intermediate Compound ◽

Mononuclear Complex ◽

Synthetic Process ◽

X Ray ◽

Pt Complex ◽

Isolation And Characterization ◽

Physical Measurements ◽

Pt Cluster

An intermediate compound of a tetranuclear Pt–Fe⋯Fe–Pt complex in the synthetic process to form a trinuclear Pt–Fe–Pt complex by mixing the “amidate-hanging” Pt mononuclear complex and iron sources was successfully isolated and characterized by single-crystal X-ray analysis and several physical measurements.

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Isolation and Characterization of Lactic Acid Bacteria in Philippine Fermented Milkfish Chanos chanos-Rice Mixture (Burong Bangus)

Current Research in Nutrition and Food Science Journal ◽

10.12944/crnfsj.6.2.24 ◽

2018 ◽

Vol 6 (2) ◽

pp. 500-508

Author(s):

Julie Ann A. Arcales ◽

Garner Algo L.Alolod

Keyword(s):

Lactic Acid ◽

Lactic Acid Bacteria ◽

Enzymatic Activity ◽

Antimicrobial Property ◽

Food Products ◽

The Philippines ◽

Lactobacillus Delbrueckii ◽

Bacterial Strains ◽

Isolation And Characterization

Isolation and characterization of bacteria in food products are important to determine and distinguish the beneficial or harmful effects of microbiota in certain samples. Lactic acid bacteria in food products had long been associated to good factors as food preservatives and with added fermentation metabolites. This study isolated and characterized lactic acid bacteria from burong bangus. The culture and purification process of bacteria isolation resulted to 4 strains of lactic acid bacteria namely Enterococcus faecalis, Tetragenococcus muriaticus, Lactobacillus delbrueckii subp. delbrueckii and Carnobacterium divergens. High enzymatic activity were observed with E. faecalis particularly on lipase and protease assay. While C. divergens have no enzymatic activity against lipase, protease, amylase and cellulase. The antimicrobial property of L. delbrueckii is only susceptible to amoxicillin unlike the other three bacteria isolates. No antagonistic activity were observed with the four bacterial strains against Bacillus subtilis, Staphylococcus aureus and Escherichia coli. The result of this study showed promising benefits to the industry especially in developing countries like the Philippines because population are not yet so aware of this organisms and the benefits that can be derived through their consumption.

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