scholarly journals Responses of phytochelatin and proline-related genes expression associated with heavy metal stress in Solanum lycopersicum

2019 ◽  
Vol 78 (1) ◽  
pp. 9-16 ◽  
Author(s):  
Dursun Kısa
Keyword(s):  
Gene Expression ◽  
Heavy Metal ◽  
Heavy Metal Stress ◽  
Proline Content ◽  
Tomato Plants ◽  
Metal Chelating ◽  
Genes Expression ◽  
Adverse Environmental Conditions ◽  
Stress Related Genes ◽  
High Doses

Abstract The expression of stress related-genes against adverse environmental conditions has essential importance for plants. This study, using RT-qPCR, determined the expression of P5CS and PCS genes to investigate their roles in the leaves of tomato plants grown under heavy metal conditions. The expression of the PCS1 gene is significantly induced under such conditions. Transcript expression of P5CS1, a gene responsible for proline synthesis, changed depending on heavy metal doses; treatments of Cu (20 and 50 ppm), Cd and Pb (10 and 20 ppm) remarkably increased P5CS1 expression. However, the P5CS1 gene expression at 10 ppm dose of Cu and 50 ppm doses of Pb and Cd was not significantly different from that in control plants. The metal-chelating potency of the extract of tomato leaves exposed to Pb and Cd was higher than that of untreated plants. The proline content as assessed in the leaves of stressed plants was significantly increased by applications of 10 and 20 ppm of Cd and Pb, and high doses of Cu. In addition, the results showed that the proline content had a positive correlation with the P5CS1 gene expression in tomato leaves under application of these tree heavy metals and that there was a positive relation between the PCS1 gene expression and metal-chelating ability of Cd-stressed plants.

Biochemical profile and gene expression of Clarias gariepinus as a signature of heavy metal stress

2020 ◽  
Vol 264 ◽  
pp. 114693
Author(s):  
Sadiya Binte Swaleh ◽  
Umarah Zahoor Banday ◽  
Moneeb-Al Asadi ◽  
Nazura Usmani
Keyword(s):  
Gene Expression ◽  
Heavy Metal ◽  
Clarias Gariepinus ◽  
Heavy Metal Stress ◽  
Metal Stress ◽  
Biochemical Profile

scholarly journals Variations in the antioxidant and free radical scavenging under induced heavy metal stress expressed as proline content in chickpea

2019 ◽  
Vol 25 (3) ◽  
pp. 683-696 ◽  
Author(s):  
Sameer Suresh Bhagyawant ◽  
Dakshita Tanaji Narvekar ◽  
Neha Gupta ◽  
Amita Bhadkaria ◽  
Kirtee Kumar Koul ◽  
...  
Keyword(s):  
Heavy Metal ◽  
Free Radical ◽  
Radical Scavenging ◽  
Heavy Metal Stress ◽  
Free Radical Scavenging ◽  
Proline Content ◽  
Metal Stress

scholarly journals Transgenerational memory of gene expression changes induced by heavy metal stress in rice (Oryza sativa L.)

2019 ◽  
Vol 19 (1) ◽  
Author(s):  
Weixuan Cong ◽  
Yiling Miao ◽  
Lei Xu ◽  
Yunhong Zhang ◽  
Chunlei Yuan ◽  
...  
Keyword(s):  
Gene Expression ◽  
Heavy Metal ◽  
Oryza Sativa ◽  
Oryza Sativa L ◽  
Heavy Metal Stress ◽  
Metal Stress

scholarly journals Selection and validation of reference genes for normalization of qRT-PCR data to study the cannabinoid pathway genes in industrial hemp

PLoS ONE ◽  
2021 ◽  
Vol 16 (12) ◽  
pp. e0260660
Author(s):  
Michihito Deguchi ◽  
Shobha Potlakayala ◽  
Zachary Spuhler ◽  
Hannah George ◽  
Vijay Sheri ◽  
...  
Keyword(s):  
Gene Expression ◽  
Heavy Metal ◽  
Reference Genes ◽  
Expression Patterns ◽  
Heavy Metal Stress ◽  
Future Research ◽  
Qrt Pcr ◽  
Differential Gene ◽  
Expression Characterization ◽  
Industrial Hemp

There has been significant interest in researching the pharmaceutical applications of Industrial hemp since its legalization three years ago. The crop is mostly dioecious and known for its production of phytocannabinoids, flavonoids, and terpenes. Although many scientific reports have showed gene expression analysis of hemp through OMICs approaches, unreliable reference genes for normalization of qRT-PCR data make it difficult to validate the OMICs data. Four software packages: geNorm, NormFinder, BestKeeper, and RefFinder were used to evaluate the differential gene expression patterns of 13 candidate reference genes under osmotic, heavy metal, hormonal, and UV stresses. EF-1α ranked as the most stable reference gene across all stresses, TUB was the most stable under osmotic stress, and TATA was the most stable under both heavy metal stress and hormonal stimuli. The expression patterns of two cannabinoid pathway genes, AAE1 and CBDAS, were used to validate the reliability of the selected reference genes. This work provides useful information for gene expression characterization in hemp and future research in the synthesis, transport, and accumulation of secondary metabolites.

scholarly journals PGPR-Mediated Plant Growth Attributes and Metal Extraction Ability of Sesbania sesban L. in Industrially Contaminated Soils

Agronomy ◽  
2021 ◽  
Vol 11 (9) ◽  
pp. 1820
Author(s):  
Nida Zainab ◽  
Amna ◽  
Amir Abdullah Khan ◽  
Muhammad Atif Azeem ◽  
Baber Ali ◽  
...  
Keyword(s):  
Heavy Metal ◽  
Antioxidant Enzymes ◽  
Plant Growth ◽  
Contaminated Soils ◽  
Root Length ◽  
Heavy Metal Stress ◽  
Proline Content ◽  
Shoot Length ◽  
Metal Stress ◽  
Sesbania Sesban

The release of harmful wastes via different industrial activities is the main cause of heavy metal toxicity. The present study was conducted to assess the effects of heavy metal stress on the plant growth traits, antioxidant enzyme activities, chlorophyll content and proline content of Sesbania sesban with/without the inoculation of heavy-metal-tolerant Bacillus gibsonii and B. xiamenensis. Both PGP strains showed prominent ACC-deaminase, indole acetic acid, exopolysaccharides production and tolerance at different heavy metal concentrations (50–1000 mg/L). Further, in a pot experiment, S. sesban seeds were grown in contaminated and noncontaminated soils. After harvesting, plants were used for the further analysis of growth parameters. The experiment comprised of six different treatments. The effects of heavy metal stress and bacterial inoculation on the plant root length; shoot length; fresh and dry weight; photosynthetic pigments; proline content; antioxidant activity; and absorption of metals were observed at the end of the experiment. The results revealed that industrially contaminated soils distinctly reduced the growth of plants. However, both PGPR strains enhanced the root length up to 105% and 80%. The shoot length was increased by 133% and 75%, and the fresh weight was increased by 121% and 129%. The proline content and antioxidant enzymes posed dual effects on the plants growing in industrially contaminated soil, allowing them to cope with the metal stress, which enhanced the plant growth. The proline content was increased up to 190% and 179% by the inoculation of bacterial strains. Antioxidant enzymes, such as SOD, increased to about 216% and 245%, while POD increased up to 48% and 49%, respectively. The results clearly show that the utilized PGPR strains might be strong candidates to assist S. sesban growth under heavy metal stress conditions. We highly suggest these PGPR strains for further implementation in field experiments.

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