scholarly journals The Effect of Halo- and Hydro-Priming on Germination Characteristics of Millet Seeds Under Salinity Stress

2014 ◽  
Vol 47 (2) ◽  
pp. 41-48 ◽  
Author(s):  
M.A. Aghbolaghi ◽  
M. Sedghi

Abstract Low seed germination and seedling emergence is one of the main problems in saline areas. Seed priming technique has been known as a challenge to improve germination and seedling emergence under different environmental stresses. The objective of this research was to evaluate the effect of seed priming with NaCl and water on growth and seed reserve utilization of millet seeds under salinity stress. Treatments were combinations of four levels of salinity stress (0, ⊟4, ⊟8 and ⊟12 bar) and three levels of seed priming and control with three replicates. Results showed that with increase in salinity stress, germination components such as germination percentage, germination index, mean time to germination, normal seedling percentage, seedling length, seedling dry weight of utilized (mobilized) seed and seed reserve utilization efficiency decreased, but seed priming showed lower reduction. The highest germination characteristics and seed reserve utilization was obtained by halo-priming in control conditions. It is concluded that priming results in improvement in germination components of millet in salinity stress conditions.

2014 ◽  
Vol 47 (2) ◽  
pp. 61-68 ◽  
Author(s):  
S.A. Tabatabaei ◽  
S.M. Naghibalghora

Abstract Objective of this study was to evaluate the effect of salinity stress on germination characteristics and biochemical changes of sesame seeds. Salinity stress at osmotic potentials of 0 (as control), ⊟3, ⊟6, ⊟9 and ⊟12 bar were adjusted using NaCl before the start of the experiment. Our results showed that, the effect of salinity stress for all traits was significant. By increases of salinity stress, germination percentage, germination, normal seedling percentage, seedling length and dry weight were reduced the ascorbate peroxidase and catalase activity, also proline content were at minimum at control and increased with increase in salinity stress, expressed by the osmotic potential.


2014 ◽  
Vol 47 (3) ◽  
pp. 39-45
Author(s):  
S.A. Tabatabaei

Abstract Seed germination and seedling growth are critical stages in the life cycle of a plant, especially under adverse abiotic stresses. Seed germination negatively affected by stress conditions. Seed priming techniques have been used to increase germination characteristics and improve germination uniformity in more field crops under stressed conditions. This experimental aimed to evaluate the effect of salinity and halo-priming on seed reserve utilization and seed germination of wheat seeds. For create salinity stress, NaCl in osmotic levels at 0 (as control), -4, -8, - 12 and -16 bar was used. Seeds of were pretreated with halo-priming at 10 °C for 24 h and water at 10 °C for 24 h. Our results showed that treatment×drought interaction on these traits: germination percentage, weight of utilized (mobilized) seed, seed reserve utilization efficiency, seedling dry weight and seed reserve depletion percentage were significant. The highest germination percentage was obtained from halo priming in control conditions. Thus priming improved study traits in wheat under salinity stress. Priming increased germination percentage and seed reserve utilization as compared to the unprimed seeds. The highest germination percentage and seed reserve utilization were as obtained from halo priming in control conditions.


2014 ◽  
Vol 47 (1) ◽  
pp. 23-29
Author(s):  
S.A. Tabatabaei

ABSTRACT In order to investigate salinity stress on seed reserve utilization and seedling growth of treated seeds of barley (Hordeum vulgare L.), an experiment was carried out. Factorial experiment was carried out in completely randomized design with three replicates. To create salinity stress, NaCl in osmotic levels at 0 (as control), -4, -8, -12 and -16 bar were used. For seed priming, gibberellin (GA) 50 ppm was used. Our results showed that treatment × drought interaction on these traits: germination percentage, weight of utilized (mobilized) seed, seed reserve utilization efficiency, seedling dry weight and seed reserve depletion percentage were significant. The highest germination percentage, weight of utilized (mobilized) seed, seed reserve utilization efficiency, seedling dry weight and seed reserve depletion percentage were attained from priming by gibberellin at control conditions. Thus, priming increased characteristics as compared to the unprimed. Priming improved seed reserve utilization such as: weight of utilized (mobilized) seed reserve, seed reserve depletion percentage, seed reserve utilization efficiency and seedling growth in barley under salinity stress.


2015 ◽  
Vol 48 (3) ◽  
pp. 33-41
Author(s):  
M. Khajeh ◽  
S.A. Tabatabaei ◽  
O. Ansari ◽  
F. Sharif Zadeh

AbstractSeed priming is one of the methods that can be taken to counteract the adverse effects of abiotic stress, also Seed priming treatments have been used to reduce the damage of aging and invigorate their performance in many crops. Objective of this study was to evaluation the effect of gibberellin on germination characteristics and antioxidant enzymes of safflower seeds after aging. Experimental design was a factorial one with complete randomized design with three replications. The first factor was priming by gibberellin (0 and 50 ppm) and non-primed seeds (control) and the secondary factor was combinations of four levels of aging (0, 1, 3 and 5 days of aging, at 43°C). The results showed that the priming and aging effects on germination percentage, germination index, normal seedling percentage, seedling length, vigor index, catalase and ascorbate peroxidase were significant, but on mean time to germination not significant. Results showed that, the highest germination characteristics such as; germination percentage, germination index, normal seedling percentage, seedling length, vigor index, catalase and ascorbate peroxidase were attained from priming by gibberellins, under non aged conditions. Also, our results indicated that seed aging is related to decrease of enzymes activity and may contribute to low seed germination efficiency, also priming increases enzyme activity and increases enzyme activity with priming treatment may contribute to improve germination characteristics. The general decreases in enzyme activity in the seed lowers the respiratory capacity, which in turn lowers both the energy (ATP) and assimilates supply of the germinating seed.


2012 ◽  
Vol 45 (3) ◽  
pp. 53-62 ◽  
Author(s):  
O. Ansari ◽  
F. Sharif Zadeh

Abstract Seed priming techniques have been used to increase germination, improve germination uniformity and seedling establishment under stressed conditions. Seed priming was used in rye mountain (Secale montanum) to increase seed germination and tolerance on stress exposure (Cold stress). Rye seeds were treated with water (Hydro priming) and polyetelyn glycol 6000 (Osmo priming). The effect of priming was assessed on germination characteristics under cold stress (at 3°C) for 7 days. Analyze of variance for hydro priming showed that temperature × time of priming interaction was significantly (P < 0.01) for all traits under cold stress and for osmo priming showed that Concentration of PEG × Temperature × Time of priming interaction was significantly (P < 0.01) for all traits under cold stress except seedling dry weight (SDW). For hydro priming the highest germination percentage (GP) was attained from hydro priming at 15±1°C for 16 h (70%), whereas the highest normal seedling percentage (NSP) (22.5%), germination index (GI) (8.57), seedling vigor index (SVI) (38.9) and seedling length (SL) (1.7 cm) were in the hydro priming at 10±1°C for 16 h. For osmo priming the highest germination percentage (GP) (80 and 80.5%), normal seedling percentage (NSP) (48 and 45%), seedling vigor index (SVI) (101.26 and 85.25) and seedling length (SL) (2.11 and 1.89 cm) observed in the osmo priming with concentrations of -9 and -15 bar PEG at 15±1°C for 24 h.


HortScience ◽  
2000 ◽  
Vol 35 (5) ◽  
pp. 907-909 ◽  
Author(s):  
Wallace G. Pill ◽  
Elizabeth A. Kilian

`Moss Curled' seeds of parsley (Petroselinum crispum L.) were primed osmotically in polyethylene glycol or matrically in fine, exfoliated vermiculite at –0.5 MPa for 4 or 7 days at 20 or 30 °C with 0 or 1 mm GA3. All priming treatments stimulated and hastened germination. Matric priming resulted in greater germination (89%) than osmotic priming (83%) when seeds were primed for 7 days at 30 °C, but priming agent had no effect on germination percentage following priming at 20 °C or for 4 days. In seeds primed for 4 days at 20 or 30 °C, matric priming hastened germination more than did osmotic priming. Germination was generally less synchronous with matric than with osmotic priming. Increasing priming time from 4 to 7 days increased the rate of germination, but increased germination synchrony only when seeds were primed at 20 °C. Inclusion of 1 mm GA3 during priming had little or no effect on germination. All matric priming treatments (other than 4-day priming) were repeated to assess seedling emergence in a greenhouse (25°C day/22 °C night). Priming increased the percentage, rate and synchrony of emergence, and increased hypocotyl length at 3 weeks after planting. Priming at 30 °C with 1 mm GA3 resulted in the greatest emergence percentage, hypocotyl length, and shoot dry weight. We conclude that matric priming is a satisfactory alternative to osmotic priming of parsley seeds. Chemical name used: gibberellic acid (GA3).


2015 ◽  
Vol 9 (1) ◽  
pp. 6-11
Author(s):  
Abolfazl Masoumi Zavariyan ◽  
Mojtaba Yousefi Rad ◽  
Mohsen Asghari

For investigation of the effect of seed priming by kinetin on germination indices and proline activity of Silybum marianum  L. under drought stress, factorial experiment based on completely randomized design with two factor includingdifferent concentrations of kinetin (0, 10 and 20 ppm) and different levels of drought stress including 0, -6 and -12 barat three replicates was carried out. According to the results, drought stress and kinetin individualy cause to decreases of germination percentage, germination rate, seedling length, seed vigor and seedling dry weight but uses of kinetin in drought stress condition improvment of them. Also, drought stress cause to increases of proline content and catalase activity. Therefore, seed priming with 10 ppm kinetin at drought stress was improved the germination indices of Silybum marianum L.DOI: http://dx.doi.org/10.3126/ijls.v9i1.11919 International Journal of Life Sciences Vol.9(1) 2015 6-11


2021 ◽  
Vol 911 (1) ◽  
pp. 012086
Author(s):  
Ramlah Arief ◽  
Fauziah Koes ◽  
Oom Komalasari ◽  
Fatmawati

Abstract Seed priming or invigoration is presowing treatments of improving germination aimed to reduce the time from sowing to emergence and improving emergence uniformity. Research on seed priming was conducted Indonesian Cereal Research Institute (ICERI) seed laboratory from January until March 2021 to evaluate the effect of seed priming on sorghum seed vigor. Seed quality parameters includes germination percentages, germination rate, shoot and primary root length, seedling dryweight, Sorghum seed variety of Suri 4 were used as seed material. Results showed that among the priming treatments that give positive effect on germination percentage, germination rate, seedling dry weight were priming treatment with KNO3 1.5%.


2015 ◽  
Vol 47 (4) ◽  
pp. 41-48 ◽  
Author(s):  
E. Ghasemi ◽  
M. Goodarzian Ghahfarokhi ◽  
B. Darvishi ◽  
Z. Heidari Kazafi

Abstract Seed aging is an important problem in developing countries which seeds stored in inappropriate condition. Delayed germination, reduced normal seedling percentage and changed antioxidant enzymes activity are some indications of aged seeds. Priming is a technique applied before planting and can improve seed characteristics especially under abiotic stress conditions. The main objective of the study was to evaluate aging effect on seed quality and to study the interaction between seed aging and seed priming. A factorial experiment based on completely randomized design with three replicates was conducted. The experimental treatments included cultivar, priming and aging. Results showed that seed aging reduced germination percentage, germination index, seedling length, normal seedling percentage, seedling dry weight, catalase and ascorbate peroxidase activity and increased the germination mean time and electrical conductivity of seeds. The highest germination percentage, germination index, seedling length, normal seedling percentage, seedling dry weight, catalase and ascorbate peroxidase activity and the minimum germination mean time and electrical conductivity of seeds were attained from hydro-priming treatment under non aged condition. Hydro-priming improved aged seeds quality and increased enzymes activity. Therefore, priming is a technique can be applied to improve aged seeds germination and seedling characteristics.


HortScience ◽  
1996 ◽  
Vol 31 (6) ◽  
pp. 912D-912
Author(s):  
Ahmet Korkmaz ◽  
Wallace Pill

Achievement of head size uniformity at final harvest reduces loss and increases profitability for the hydroponic lettuce grower. Shoot fresh weight of `Cortina' lettuce (Lactuca sativa L.) at 7 or 21 days after planting (DAP) was inversely proportional to the number of days required for seedling emergence, and was greater for raw than for pelleted seeds. Head fresh weight at final harvest (61 DAP) was directly proportional to seedling length at 21 DAP, but raw and pelleted seeds produced equal head weights. Thus, initial seed (seedling) vigor differences were maintained to final harvest. Osmotic seed priming (–1.5 MPa KH2PO4, 20 h, dark) led to increased germination rate at 15, 25, and 35C; had no effect on germination synchrony; and increased germination percentage only at 35C. Covering raw or pelleted seeds sown in depressions of the phenolic foam trays with fine (No. 5) vermiculite compared to leaving the seeds uncovered, and soaking the trays in hydroponic solution rather than water, increased seedling shoot fresh weights. Seeds sown on their first day of germination or primed seeds gave greater seedling shoot fresh weights than pelleted seeds. However, the more uniform seedling shoot fresh weights from germinated seeds than from primed seeds was associated with more rapid and synchronous seedling emergence.


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