scholarly journals Germination and Emergence of Parsley in Response to Osmotic or Matric Seed Priming and Treatment with Gibberellin

HortScience ◽  
2000 ◽  
Vol 35 (5) ◽  
pp. 907-909 ◽  
Author(s):  
Wallace G. Pill ◽  
Elizabeth A. Kilian
Keyword(s):  
Polyethylene Glycol ◽  
Gibberellic Acid ◽  
Seedling Emergence ◽  
Dry Weight ◽  
Seed Priming ◽  
Germination Percentage ◽  
Petroselinum Crispum ◽  
Hypocotyl Length ◽  
Shoot Dry Weight ◽  
Emergence Percentage

`Moss Curled' seeds of parsley (Petroselinum crispum L.) were primed osmotically in polyethylene glycol or matrically in fine, exfoliated vermiculite at –0.5 MPa for 4 or 7 days at 20 or 30 °C with 0 or 1 mm GA3. All priming treatments stimulated and hastened germination. Matric priming resulted in greater germination (89%) than osmotic priming (83%) when seeds were primed for 7 days at 30 °C, but priming agent had no effect on germination percentage following priming at 20 °C or for 4 days. In seeds primed for 4 days at 20 or 30 °C, matric priming hastened germination more than did osmotic priming. Germination was generally less synchronous with matric than with osmotic priming. Increasing priming time from 4 to 7 days increased the rate of germination, but increased germination synchrony only when seeds were primed at 20 °C. Inclusion of 1 mm GA3 during priming had little or no effect on germination. All matric priming treatments (other than 4-day priming) were repeated to assess seedling emergence in a greenhouse (25°C day/22 °C night). Priming increased the percentage, rate and synchrony of emergence, and increased hypocotyl length at 3 weeks after planting. Priming at 30 °C with 1 mm GA3 resulted in the greatest emergence percentage, hypocotyl length, and shoot dry weight. We conclude that matric priming is a satisfactory alternative to osmotic priming of parsley seeds. Chemical name used: gibberellic acid (GA3).

scholarly journals Germination and Emergence of Parsley in Response to Osmotic or Matric Seed Priming and Treatment with Gibberellin

2001 ◽  
Vol 11 (1) ◽  
pp. 152
Author(s):  
Wallace G. Pill ◽  
Elizabeth A. Kilian
Keyword(s):  
Polyethylene Glycol ◽  
Gibberellic Acid ◽  
Seedling Emergence ◽  
Dry Weight ◽  
Seed Priming ◽  
Germination Percentage ◽  
Petroselinum Crispum ◽  
Hypocotyl Length ◽  
Shoot Dry Weight ◽  
Emergence Percentage

`Moss Curled' seeds of parsley (Petroselinum crispum L.) were primed osmotically in polyethylene glycol or matrically in fine, exfoliated vermiculite at -0.5 MPa for 4 or 7 days at 20 or 30 °C with 0 or 1 mm GA3. All priming treatments stimulated and hastened germination. Matric priming resulted in greater germination (89%) than osmotic priming (83%) when seeds were primed for 7 days at 30 °C, but priming agent had no effect on germination percentage following priming at 20 °C or for 4 days. In seeds primed for 4 days at 20 or 30 °C, matric priming hastened germination more than did osmotic priming. Germination was generally less synchronous with matric than with osmotic priming. Increasing priming time from 4 to 7 days increased the rate of germination, but increased germination synchrony only when seeds were primed a t 20 °C. Inclusion of 1 mm GA3 during priming had little or no effect on germination. All matric priming treatments (other than 4-day priming) were repeated to assess seedling emergence in a greenhouse (25°C day/22 °C night). Priming increased the percentage, rate and synchrony of emergence, and increased hypocotyl length at 3 weeks after planting. Priming at 30 °C with 1 mm GA3 resulted in the greatest emergence percentage, hypocotyl length, and shoot dry weight. We conclude that matric priming is a satisfactory alternative to osmotic priming of parsley seeds. Chemical name used: gibberellic acid (GA3).

scholarly journals GIBBERELLIC ACID DURING PRIMING OF PURPLE CONEFLOWER [ECHINACEA PURPUREA (L) MOENCH.] SEEDS IMPROVES GERMINATION AND SEEDLING EMERGENCE

HortScience ◽  
1995 ◽  
Vol 30 (2) ◽  
pp. 186c-186
Author(s):  
Janine O. Haynes ◽  
Wallace G. Pill
Keyword(s):  
Gibberellic Acid ◽  
Germination Rate ◽  
Seedling Emergence ◽  
Dry Weight ◽  
Echinacea Purpurea ◽  
Germination Percentage ◽  
Shoot Dry Weight ◽  
Greenhouse Study ◽  
Purple Coneflower ◽  
Percentage Points

Purple coneflower seeds following priming (-0.04 MPa, 10 days, 15C, darkness) osmotically in polyethylene glycol 8000 (PEG) or matrically in expanded no. 5 vermiculite had greater germination rate and synchrony at continuous 20C or 30C than untreated seeds, but germination percentage was unaffected. Inclusion of 5.5 × 10-2 M gibberellic acid (GA3 as ProGibb Plus 2X, Abbott Laboratories, N. Chicago, Ill.) further improved germination rate and synchrony at 20C, but not at 30C. In a greenhouse study (30C day/27C night, July-August natural light), seeds primed in PEG or vermiculite containing G A3 compared to untreated seeds had 6 percentage points higher maximum emergence (ME), 3.3 fewer days to 50% ME, 1.9 fewer days between 10% and 90% ME, 116% greater shoot dry weight, and 125% longer leaves at 16 days after planting in peat-lite. Inclusion of ethephon (0.01 m, as Florel) either alone or with GA3 during priming provided no benefit to seed germination or seedling emergence. Moistened vermiculite substituted for PEG solution as a priming medium for purple coneflower seeds, the priming benefit on seedling emergence and growth being enhanced by 5.5 × 10-2m G A3 inclusion in the priming media.

scholarly journals The Effect of Halo- and Hydro-Priming on Germination Characteristics of Millet Seeds Under Salinity Stress

2014 ◽  
Vol 47 (2) ◽  
pp. 41-48 ◽  
Author(s):  
M.A. Aghbolaghi ◽  
M. Sedghi
Keyword(s):  
Salinity Stress ◽  
Seedling Emergence ◽  
Dry Weight ◽  
Seed Priming ◽  
Germination Percentage ◽  
Utilization Efficiency ◽  
Seedling Length ◽  
Germination Characteristics ◽  
Normal Seedling ◽  
Four Levels

Abstract Low seed germination and seedling emergence is one of the main problems in saline areas. Seed priming technique has been known as a challenge to improve germination and seedling emergence under different environmental stresses. The objective of this research was to evaluate the effect of seed priming with NaCl and water on growth and seed reserve utilization of millet seeds under salinity stress. Treatments were combinations of four levels of salinity stress (0, ⊟4, ⊟8 and ⊟12 bar) and three levels of seed priming and control with three replicates. Results showed that with increase in salinity stress, germination components such as germination percentage, germination index, mean time to germination, normal seedling percentage, seedling length, seedling dry weight of utilized (mobilized) seed and seed reserve utilization efficiency decreased, but seed priming showed lower reduction. The highest germination characteristics and seed reserve utilization was obtained by halo-priming in control conditions. It is concluded that priming results in improvement in germination components of millet in salinity stress conditions.

Seed priming with brassinolide improves lucerne (Medicago sativa L.) seed germination and seedling growth in relation to physiological changes under salinity stress

2007 ◽  
Vol 58 (8) ◽  
pp. 811 ◽  
Author(s):  
S. Zhang ◽  
J. Hu ◽  
Y. Zhang ◽  
X. J. Xie ◽  
Allen Knapp
Keyword(s):  
Salt Stress ◽  
Medicago Sativa ◽  
Salinity Stress ◽  
Seedling Growth ◽  
Dry Weight ◽  
Seed Priming ◽  
Germination Percentage ◽  
Shoot Dry Weight ◽  
Medicago Sativa L ◽  
High Level

Salt stress is an important constraint to lucerne (Medicago sativa L.) production in many parts of the world. Seeds of 3 lucerne varieties, cvv. Victoria, Golden Empress, and Victor, were used to investigate the effects of seed priming with 5 µm/L brassinolide on germination and seedling growth under a high level of salt stress (13.6 dS/m NaCl solution). The results showed that germination percentage, germination index, and vigour index of lucerne seeds primed with brassinolide were significantly higher than those of the non-primed seeds under salinity stress in each variety. Seed priming with brassinolide significantly increased the shoot fresh weight, shoot dry weight, and root dry weight in 2 varieties, and significantly increased the root length and root vigour in each variety. It also significantly increased the activities of antioxidant enzymes, peroxidase (POD), superoxide dismutase (SOD), and catalase (CAT), in Victoria and Victor seedlings. During seedling growth, the primed seeds significantly reduced the malondialdehyde (MDA) accumulation. This suggests that priming lucerne seed with brassinolide at a suitable concentration can improve germination and seedling growth under high-saline soils.

Enhancing the germination of three fodder shrubs (Atriplex amnicola, A. nummularia, A. undulata; Chenopodiaceae): implications for the optimisation of field establishment

2006 ◽  
Vol 57 (12) ◽  
pp. 1279 ◽  
Author(s):  
J. C. Stevens ◽  
E. G. Barrett-Lennard ◽  
K. W. Dixon
Keyword(s):  
Salicylic Acid ◽  
Seed Germination ◽  
Gibberellic Acid ◽  
Seedling Emergence ◽  
Seed Priming ◽  
Germination Percentage ◽  
Controlled Conditions ◽  
Field Emergence ◽  
Signalling Compounds ◽  
Germination Requirements

Saltbush (Atriplex) species are widely grown in Australia as saltland pastures. Direct seeding practices for saltbush currently result in asynchronous and unreliable seedling establishment (5% successful establishment is not uncommon from field-sown seed). In part this may stem from a limited understanding of Atriplex seed germination requirements. This paper presents findings with 3 Atriplex species, A. amnicola (Paul G. Wilson.), A. nummularia (Lindl.), and A. undulata (D. Dietr), each of which differs in germination characteristics. For A. amnicola, the presence of light (and artificial substitution of light by 1000 ppm gibberellic acid) improved germination under controlled conditions and resulted in a 4-fold increase (70% total emergence) in field emergence of seedlings. For A. undulata, removing bracteoles increased germination under controlled conditions (~15%), with a 1.5-fold improvement in field seedling emergence (55% final emergence); however, seed priming or gibberellic acid application had no significant effect. In contrast, for A. nummularia, bracteole removal and light had minor positive effects on germination under controlled conditions, but this did not translate into improved emergence in soil or in the field. Under –0.5 MPa NaCl stress, application of gibberellic acid, salicylic acid, or kinetin to the germination medium significantly increased the final germination percentage of A. amnicola seeds (58, 16, and 14%, respectively) and improved the rate at which seeds germinated. All plant signalling compounds significantly increased final germination percentage and germination rate of A. undulata, albeit with a <10% increase at –0.5 MPa NaCl. Priming seeds with plant signalling compounds had similar effects on seed germination under low water potentials compared to direct treatment of the germination media. The effects of seed priming on Atriplex seedling emergence from saline soils varied among species. Priming with water significantly increased emergence percentage of A. amnicola but had no effect on A. nummularia and A. undulata. Gibberellic acid improved A. amnicola germination parameters only, whereas salicylic acid and kinetin improved the rate of emergence in all 3 species at various levels of salinity. This study suggests that a basic understanding of seed dormancy and germination requirements has the potential to substantially improve field emergence of saltbush species.

scholarly journals Seed Priming and Field Performance of Soybean (Glycine max L.) in Response to Water Limitation

2011 ◽  
Vol 39 (2) ◽  
pp. 186 ◽  
Author(s):  
Kazem GHASSEMI-GOLEZANI ◽  
Somayeh FARSHBAF-JAFARI ◽  
Jalil SHAFAGH-KOLVANAGH
Keyword(s):  
Field Experiment ◽  
Grain Yield ◽  
Field Performance ◽  
Dry Weight ◽  
Seed Priming ◽  
Germination Percentage ◽  
Severe Water Deficit ◽  
Glycine Max L ◽  
Emergence Percentage ◽  
Response To Water

Laboratory tests and a field experiment were carried out to evaluate the effects of priming methods on seed invigoration and field performance of soybean (cv. ‘Zan’). The field experiment was arranged as split plot based on RCB design with three replications. Irrigation treatments (I1, I2 and I3: irrigation after 70, 110 and 150 mm evaporation from class A pan) and priming methods (water, 3% KH2PO4 and 3% KNO3 for 8 h at 15±1°C) were allocated to main and sub-plots, respectively. Germination percentage, seedling dry weight and field emergence percentage decreased, but mean emergence time increased, due to seed priming. Grain yield under severe water deficit was 29.32% less than that under normal irrigation. Pods per plant, grains per plant and grain yield per plant were significantly enhanced as a result of low stand establishment caused by seed priming. Consequently, biological and grain yields per unit area and also harvest index were statistically similar for plants from primed and unprimed seeds. In general, priming methods had no any beneficial effect on laboratory and field performance of soybean seeds.

scholarly journals Seed Priming with Sorghum Water Extract Improves the Performance of Camelina (Camelina sativa (L.) Crantz.) under Salt Stress

Plants ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 749
Author(s):  
Ping Huang ◽  
Lili He ◽  
Adeel Abbas ◽  
Sadam Hussain ◽  
Saddam Hussain ◽  
...  
Keyword(s):  
Salt Stress ◽  
Water Extract ◽  
Dry Weight ◽  
Seed Priming ◽  
Stress Conditions ◽  
Crop Tolerance ◽  
Shoot Dry Weight ◽  
Mda Content ◽  
Emergence Percentage

Seed priming with sorghum water extract (SWE) enhances crop tolerance to salinity stress; however, the application of SWE under salinity for camelina crop has not been documented so far. This study evaluated the potential role of seed priming with SWE in improving salt stress tolerance in camelina. Primed (with 5% SWE and distilled water-hydropriming) and nonprimed seeds were sown under control (no salt) and salt stress (10 dS m−1) conditions. Salinity reduced camelina’s emergence and growth, while seed priming with SWE improved growth under control and stress conditions. Under salt stress, seed priming with SWE enhanced emergence percentage (96.98%), increased root length (82%), shoot length (32%), root dry weight (75%), shoot dry weight (33%), α-amylase activity (66.43%), chlorophyll content (60–92%), antioxidant enzymes activity (38–171%) and shoot K+ ion (60%) compared with nontreated plants. Similarly, under stress conditions, hydrogen peroxide, malondialdehyde (MDA) content, and shoot Na+ ion were reduced by 60, 31, and 40% by seed priming with SWE, respectively, over the nonprimed seeds. Therefore, seed priming with SWE may be used to enhance the tolerance against salt stress in camelina.

EMERGENCE AND SEEDLING GROWTH OF INBRED LINES OF CORN AT SUBOPTIMAL ROOT-ZONE TEMPERATURES

1987 ◽  
Vol 67 (2) ◽  
pp. 409-415 ◽  
Author(s):  
A. MENKIR ◽  
E. N. LARTER
Keyword(s):  
Zea Mays ◽  
Seedling Growth ◽  
Root Zone ◽  
Seedling Emergence ◽  
Correlation Coefficients ◽  
Dry Weight ◽  
Inbred Lines ◽  
Root Zone Temperature ◽  
Emergence Percentage ◽  
Zone Temperature

Based on the results of an earlier paper, 12 inbred lines of corn (Zea mays L.) were evaluated for emergence and seedling growth at three controlled root-zone temperatures (10, 14, and 18 °C). Low root-zone temperatures, 10 and 14 °C, were detrimental to emergence, seedling growth, and root growth of all inbred lines. Differential responses of inbred lines were observed within each temperature regime. The differences in seedling emergence among lines became smaller with increasing root-zone temperature, while the reverse was true for seedling dry weight. Simple correlation coefficients showed a significantly (P = 0.05) negative association between emergence percentage and emergence index (rate). Neither of these two emergence traits was significantly correlated with seedling dry weights. Seedling dry weights were significantly (P = 0.01) and positively associated with root dry weights. Two inbred lines exhibited good tolerance to low root-zone temperatures, viz. CO255 and RB214. A significant and positive correlation existed between emergence percentage at a root-zone temperature of 10 °C and field emergence in test with the same genotypes reported earlier. Selection at a root-zone temperature of 10 °C for a high percentage of seedling emergence, therefore, could be effective in identifying genotypes capable of germinating in cool soils. Furthermore, the significantly (P = 0.01) positive relationship between seedling dry weights at all root-zone temperatures and those from the field test suggest that strains with vigorous seedling growth in the field could be identified using low root-zone temperature regimes.Key words: Zea mays, root-zone temperature, cold tolerance

scholarly journals Exogenous Antioxidants Enhance Seedling Growth and Yield of Artificially Aged Cabbage and Lettuce Seeds

Horticulturae ◽  
2021 ◽  
Vol 7 (9) ◽  
pp. 274
Author(s):  
Ademola Adetunji ◽  
Sershen ◽  
Boby Varghese ◽  
Norman Pammenter
Keyword(s):  
Leaf Area ◽  
Seedling Growth ◽  
Seedling Emergence ◽  
Dry Weight ◽  
Growth And Yield ◽  
Seed Vigour ◽  
Beneficial Effects ◽  
Shoot Dry Weight ◽  
Antioxidant Defence System ◽  
Aged Seeds

Aged seeds exhibit compromised vigour in terms of germination, seedling emergence and growth, but this can to some extent be alleviated by invigoration treatments before sowing. This study aimed to investigate ageing rates and patterns in cabbage (Brassica oleraceae) and lettuce (Lactuca sativa) seeds and whether the beneficial effects of invigorating aged seeds with exogenous antioxidants translate to enhanced seedling performance. Seeds were artificially aged to 25% viability before soaking in 0.4 mM glycerol, 0.6 mM GSH and 0.2 mM trolox for cabbage, and 0.6 mM glycerol, GSH and trolox for lettuce; deionised water served as a control. After 14 days of sowing, seedling emergence percentage, mean emergence time, mean daily emergence, and time taken to 25% emergence were computed. Seedling vigour index, root and shoot dry weight, root:shoot ratio, leaf area, leaf area ratio, and leaf chlorophyll content were assessed 6 weeks after sowing. Furthermore, the photosynthetic rate (Pn), stomatal conductance (Gs), transpiration rate (E), and chlorophyll fluorescence were measured 6 weeks after sowing. Notably, ageing resulted in the loss of seed vigour and viability at higher rates in lettuce than cabbage. Seed pretreatment with glycerol promoted seedling growth in both species and shoot dry weight in lettuce, while glycerol and GSH enhanced Pn, Gs and E in lettuce. Trolox also enhanced Pn and E in lettuce. The beneficial effects of the antioxidant treatments are thought to be associated with the protection of photosystems from oxidative stress and/or stimulation of enzymes involved in photosynthesis, possibly through an enhanced antioxidant defence system during the early development stages when seedlings are particularly vulnerable to stress.

scholarly journals Emergence and Shoot Growth of Cosmos and Marigold from Paclobutrazol-treated Seed

2001 ◽  
Vol 19 (1) ◽  
pp. 11-14 ◽  
Author(s):  
Wallace G. Pill ◽  
James A. Gunter
Keyword(s):  
Growth Medium ◽  
Shoot Growth ◽  
Dry Weight ◽  
Tagetes Patula ◽  
Shoot Height ◽  
Shoot Dry Weight ◽  
Grade 5 ◽  
Treated Seed ◽  
Emergence Percentage ◽  
And Control

Abstract This study was conducted to determine whether treating seeds of ‘Sensation Mixed’ cosmos (Cosmos bipinnatus Cav.) and ‘Bonanza Gold’ marigold (Tagetes patula L.) with paclobutrazol (PB) could suppress seedling growth. Seeds were soaked in solutions of 0, 500 or 1000 mg PB/liter (ppm PB) for 16 hours at 25C (77F) or they were primed [−0.5 MPa (−5 bars) for 7 days at 20C (68F)] in Grade 5 exfoliated vermiculite moistened with 0, 500 or 1000 ppm PB solltuions. Soaked and primed seeds were dried for 1 day at 19C (65F) and 25% relative humidity. These seeds and control (non-treated) seeds were sown into plug cells containing peat-lite. Increasing PB concentration decreased cosmos shoot height at 32 days after planting (DAP), but decreased emergence percentage, responses that were more pronounced with priming than with soaking. A 1 ppm PB growth medium drench [30 ml/cell(0.2 mg PB/cell)] and, to a greater extent a 10 mg PB/liter (ppm PB) shoot spray [2 ml/shoot (0.02 mg PB/shoot)], both applied at 10 DAP, resulted in greater cosmos shoot height suppression at 32 DAP than treatment of seeds with 1000 ppm PB. Soaking marigold seeds in 1000 ppm PB failed to decrease shoot height below those of plants from non-treated seeds at 32 DAP. However, exposure to 1000 ppm PB during priming of marigold seeds resulted in a similar shoot height suppression (13%) as the growth medium drench, and similar shoot dry weight reduction (21%) as the shoot spray. Suppression of shoot growth by this seed treatment was short-term since by five weeks after transplanting into 15 cm (6 in) pots, only marigold plants that had received the growth medium drench or shoot spray were smaller than those of control plants. Treating marigold seeds with 1000 mg ppm PB used about one-fifth the PB used to drench the growth medium.

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