scholarly journals Mastitis Pathogens Isolated from Raw Milk Samples on Sheep Farms Situated in Marginal Parts of Slovakia

2018 ◽  
Vol 62 (3) ◽  
pp. 56-61
Author(s):  
F. Zigo ◽  
M. Vasiľ ◽  
L. Takáč ◽  
M. Zigová ◽  
J. Elečko
Keyword(s):  
Health Status ◽  
Staphylococcus Epidermidis ◽  
Raw Milk ◽  
Dairy Herds ◽  
Coagulase Negative Staphylococci ◽  
Intramammary Infection ◽  
Milk Samples ◽  
California Mastitis Test ◽  
Staphylococcus Intermedius ◽  
Mastitis Pathogens

Abstract Relatively large parts of the Slovak territory are marginal regions, which in terms of the economy of ruminant rearing can effectively produce animal commodities only occasionally. The aim of this study was to evaluate the occurrence and aetiology of mastitis during one milking season in two sheep dairy herds of 224 (A) and 270 (B) ewes situated in the marginal parts of Slovakia. A complex examination of health status of ewes based on: the clinical examination of the udder, macroscopic evaluation of milk with the California mastitis test (CMT) and the bacteriological analysis of raw milk samples from individual halves were carried out at the beginning (April) and at the end (September) of the milking season. The prevalence of intramammary infection (IMI) in the herds of ewes (A and B) were 19.1 % and 14.3 %, respectively. In both herds we confirmed predominantly the subclinical forms of IMI. The highest percentages of aetiological agents were determined for coagulase negative staphylococci (CoNS), such as Staphylococcus chromogenes, Staphylococcus schleiferi, Staphylococcus epidermidis, Staphylococcus xylosus, Staphylococcus piscifermentans and Staphylococcus intermedius, the occurrences of which were determined in 41 (48.2 %) and 37 (47.9 %) positive milk samples, respectively.

scholarly journals Molecular identification of coagulase-negative staphylococci isolated from bovine mastitis and detection of β-lactam resistance

2015 ◽  
Vol 9 (09) ◽  
pp. 1022-1027 ◽  
Author(s):  
Mariela Elizabeth Srednik ◽  
Mario Andres Grieben ◽  
Adriana Bentancor ◽  
Elida Raquel Gentilini
Keyword(s):  
Fragment Length Polymorphism ◽  
Meca Gene ◽  
Bovine Mastitis ◽  
Economic Loss ◽  
Economic Losses ◽  
Dairy Herds ◽  
Coagulase Negative Staphylococci ◽  
Milk Samples ◽  
Gap Gene ◽  
Mastitis Pathogens

Introduction: Bovine mastitis is a frequent cause of economic loss in dairy herds. Coagulase-negative staphylococci (CoNS) are increasing in importance as causeof bovine intramammary infection (IMI) throughout the world in recent years. CoNShave been isolated from milk samples collected from cows with clinical andsubclinical mastitis in several countries. Identification of mastitis pathogensis important when selection appropriate antimicrobial therapy.Methodology: A total of 93 strains of Staphylococcusspp isolated from bovine clinical andsubclinical mastitis in Argentina during 2010-2013 were identified by Restriction Fragment Length Polymorphism (RFLP)-PCR using the gap gene. The isolates were tested by PCR for the presence of blaZ gene and mecA gene and were tested by disk diffusion for the susceptibilityto penicillin and cefoxitin. Results: The most common CoNS species was S.chromogenes 46.2% (43/93), followed by S. devriesei 11.8% (11/93) and S. haemolyticus 9.7% (9/93). The blaZ gene was detected in 19 (20.4%) but only 16 (17.2%) isolates were resistant to penicillin; the mecA was detected in6 (6.5%) isolates but only 4 (4.3) were resistant to cefoxitin. The 6 mecA-positive isolates showed oxaxillinMICs ≥ 0.5 μg/ml. Discussion: CoNSare important minor mastitis pathogens and can be the cause of substantial economic losses. Despite the low resistance to PEN in Argentina, the presenceof MR isolates found in this study emphasize the importance of identificationof CoNS when an IMI is present because of the potentially risk of lateraltransfer of resistance genes between staphylococcal species.

Prevalence of pathogens in milk samples of dairy cows with clinical mastitis and in heifers at first parturition

2009 ◽  
Vol 76 (2) ◽  
pp. 179-187 ◽  
Author(s):  
Bernd-Alois Tenhagen ◽  
Inken Hansen ◽  
Annette Reinecke ◽  
Wolfgang Heuwieser
Keyword(s):  
Dairy Cows ◽  
Clinical Mastitis ◽  
Clinical Samples ◽  
Dairy Herds ◽  
Coagulase Negative Staphylococci ◽  
Staph Aureus ◽  
Milk Samples ◽  
Negative Results ◽  
On Farm ◽  
Mastitis Pathogens

Prevalence of mastitis pathogens in milk samples from dairy cows and heifers was studied over a period of 1 year (Aug 2005–Aug 2006) in ten dairy herds in Germany. Milk samples (n=8240) were collected from heifers without clinical mastitis at parturition (n=6915), from primiparous cows with clinical mastitis (n=751) and from older cows with clinical mastitis (n=574). Coagulase negative staphylococci (CNS) were the predominant group of bacteria isolated (46·8% of samples) from clinically healthy quarters of primiparous cows around parturition, followed by streptococci (12·6%), coliforms (4·7%) and Staphylococcus aureus (4·0%). Thirty-three percent of samples were negative on culture (range on farm level, 12·0–46·4%). In cases of clinical mastitis in primiparous and older cows, streptococci were the predominant finding (32·1 and 39·2%) followed by CNS (27·4 and 16·4%), coliforms (10·3 and 13·1%) and Staph. aureus (10·0 and 11·7%). Negative results were obtained from 21·3% (range, 0·0–30·6%) and 19·5% (range, 0·0–32·6%) of these samples. Results indicated substantial differences in the prevalence of pathogens among herds. There was a positive within-herd correlation between the monthly prevalences for Streptococcus dysgalactiae between the three groups of samples. This correlation was also found between clinical samples of primiparous and older cows for Staph. aureus. These correlations were not found for the other pathogens. Besides herd, prevalence of pathogens was influenced by parity, type of sample and season.

scholarly journals Ocorrência de patógenos causadores de mastite subclínica no município de Jaguapitã, estado do Paraná - Brasil

2015 ◽  
Vol 36 (5) ◽  
pp. 3233
Author(s):  
Edivaldo Maziero Soethe ◽  
Mauricio Zampronio Affonso ◽  
Antonio Francisco Chaves Neto ◽  
Luiz Carlos Negri Filho ◽  
Dalton Evert Bronkhorst ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Causative Agent ◽  
Bacterial Growth ◽  
Bacterial Culture ◽  
Subclinical Mastitis ◽  
Coagulase Negative Staphylococci ◽  
Milk Samples ◽  
California Mastitis Test ◽  
Streptococcus Uberis ◽  
Staphylococcus Intermedius

<p>The aim of this research was to identify the occurrence of pathogens causing subclinical mastitis in grade B milk farms of the Jaguapitã county, state of Paraná, Brazil. California Mastitis Test (CMT) were carried out in 400 milk samples from 100 animals and 157 teats from 55 animals (55%) were positive, showed score two or higher to CMT. When these 157 positive samples to CMT were transported for bacterial culture in blood agar, 25.48% (40/157) samples showed no bacterial growth or more than two types of bacterial colonies grew, 28.03% (44/157) were Coagulase-negative staphylococci (CNS), 8.28% (13/157) were <em>Streptococcus uberis</em>, 7.64% (12/157) were <em>Staphylococcus aureus</em>, 7.64% (12/157) were <em>Corynebacterium </em>spp, 7.01% (11/157) were <em>Staphylococcus intermedius</em>, 4.46% (7/157) were <em>Staphylococcus hyicus</em>, 3.82% (6/157) were <em>Bacillus </em>spp., 2.55% (4/157) were <em>Streptococcus dysgalacteae</em>, Enterobacteria and Yeasts. We conclude that CNS is the most relevant subclinical mastitis causative agent.</p><p><strong> </strong></p>

Prevalence of intramammary infection in Dutch dairy herds

2009 ◽  
Vol 76 (2) ◽  
pp. 129-136 ◽  
Author(s):  
Otlis Sampimon ◽  
Herman W Barkema ◽  
Inge Berends ◽  
Jan Sol ◽  
Theo Lam
Keyword(s):  
Recording System ◽  
Dairy Herds ◽  
Coagulase Negative Staphylococci ◽  
Prevalence Studies ◽  
Udder Health ◽  
Intramammary Infection ◽  
Milk Samples ◽  
Lactating Cows ◽  
Streptococcus Uberis ◽  
Changes Over Time

A survey was carried out in 2003 in 49 dairy herds to determine the overall and pathogen-specific prevalence of intramammary infection (IMI) in Dutch dairy herds, and to compare the distribution with four studies performed from 1973 to 1985 in The Netherlands. Herds were randomly selected stratified over the 12 Dutch provinces, had at least 40 lactating cows and participated in the Dutch milk recording system. Quarter milk samples were collected from all 408 cows with a somatic cell count (SCC) ⩾250 000 cells/ml and 145 heifers with SCC ⩾150 000 cells/ml at the last milk test before the farm visit. Additionally, samples were collected from 519 (approximately 25%) of the remaining low-SCC cows and heifers with a SCC at the last milk test before the farm visit of <250 000 and <150 000 cells/ml, respectively. Bacterial growth occurred in 37·3% of milk samples of high-SCC cows and in 21·1% of low-SCC cows. Coagulase-negative staphylococci (CNS) were the most frequently isolated group of bacteria (10·8% of quarters) and were found in all herds. Prevalence of Staphylococcus aureus IMI was lower in 2003 than in 1973, respectively 1·8% and 6·2% of quarters. Prevalence of Streptococcus uberis and Str. dysgalactiae IMI was almost the same in the five samplings during the 30-year period, at 1·1–1·7 and 0·9–1·5%, respectively. Str. agalactiae was not found in this study. Prevalence of CNS IMI was higher in lactating heifers, while prevalence of Str. uberis, Str. dysgalactiae and penicillin-resistant Staph. aureus IMI was higher in older cows. Because distribution of pathogens changes over time, herd-level samples for bacteriological culturing must be taken regularly to monitor udder health. Additionally, national mastitis prevalence studies give important information through monitoring the national udder health status.

scholarly journals Bulk tank somatic cell count and associated microbial quality of milk from selected dairy cattle herds in Oyo State, Nigeria

2018 ◽  
Vol 7 (2) ◽  
Author(s):  
Olufemi Olatoye ◽  
Adesola Amosun ◽  
Uzo Ogbu ◽  
Yemi Okunlade
Keyword(s):  
Somatic Cell ◽  
Cell Count ◽  
Somatic Cell Count ◽  
Health Management ◽  
Development Program ◽  
Raw Milk ◽  
Human Consumption ◽  
Quality And Safety ◽  
Milk Samples ◽  
California Mastitis Test

Improvement of traditional and nomadic milk production through dairy development program in Nigeria requires routine quality and safety monitoring of milk both at herd level and milk collection centers. A total of 411 bulk raw milk samples aseptically obtained from Ibarapa, Oyo and Oke-Ogun industrial milk collection centers were subjected to California Mastitis Test (CMT), Bulk Somatic Cell Count (BSCC) and bacteriological analysis for assessment of quality and safety of milk from the herds. One hundred and seven (26.0%) of the samples were CMT positive, while 74.0% were negative to CMT. The overall mean BSCC, TAC and TCC were 1.27×103 ± cells/mL, 1.12×103± 34 cfu/mL, 97.8±9.8 cfu/mL in the CMT negative milk samples while for the strong positive samples the mean BSCC, TAC and TCC were 4.33×106 ± cells/mL, 2.35×106 ± 453 cfu/mL, 189.3±41.1 cfu/mL respectively; these were higher than the Pasteurized Milk Ordinance acceptable limits. Positive correlation was found between CMT scores and bacterial contamination and between CMT scores and SCC was recorded. About 26.0% of the samples with positive CMT could be considered unsafe due to strong correlation with microbial contamination that could result in milk borne zoonoses and public health hazards. However, a greater proportion (76.9%) of the milk with negative CMT scores could be safe for human consumption after post-harvest pasteurization. Consequently, there is need to improve handling, environmental and milking hygiene; as well as proper herd and udder health management to improve quality and safety of Nigeria dairy products.

scholarly journals THE OCCURRENCE OF BACTERIAL AGENTS CAUSING MASTITIS IN DAIRY SHEEP AND THEIR RESISTANCE TO ANTIBIOTICS

2020 ◽  
Vol 3 (7) ◽  
Author(s):  
František Zigo ◽  
Katarína Veszelits Lakticová ◽  
Mária Vargová M
Keyword(s):  
Staphylococcus Aureus ◽  
Antibiotic Resistance ◽  
High Resistance ◽  
Subclinical Mastitis ◽  
Causative Organism ◽  
Coagulase Negative Staphylococci ◽  
Isolation And Identification ◽  
Milk Samples ◽  
California Mastitis Test ◽  
Acute Form

Introduction: The aim of this study was to monitor the occurrence of bacterial agents causing mastitis, forms of mastitis and antibiotic resistance in 300 ewes on a farm in Eastern Slovakia. Material and methods: During the milking season, were performed three complex investigations including clinical examination, California Mastitis Test and laboratory analysis of milk samples. The investigations and milk samples were taken in three phases; the beginning (April), the middle (June) and the end (September) of the milking season. Results: Of all the samples (806), 225 (28.0%) were positive for bacterial pathogens. The highest incidence of mastitis (33.3%) was recorded in September, while April (23.8%) and June (25.3%) had lower incidence. The samples from September showed the highest incidence of subclinical mastitis (20.1%), with 13.2% being an acute form of mastitis. Coagulase-negative staphylococci (CNS) were identified in 61.7% of the positive samples. Especially, S. chromogenes, S. epidermidis and S. schleiferi were most frequently isolated.  Staphylococcus aureus was the causative agent in 20.0% of the positive samples and caused acute or subclinical mastitis in the affected ewes. The tested bacteria showed very high resistance to novobiocin (59.5%) and penicillin (51.4%) and high resistance to amoxycillin (35.1%). We found that 80% of Staphylococcus aureus bacteria tested for antibiotic resistance were resistant to novobiocin and 70% were resistant to penicillin. Of all tested CNS, 56.5% were resistant to novobiocin, 39.1% to penicillin, and 34.7% to amoxicillin. Conclusion: Proper isolation and identification of the causative organism play a significant role in the prevention and control of the intramammary infection. In our study, a combinations of Streptomycin, Ciprofloxacin and Tetracycline were the most effective antibiotics for the control of mastitis.

scholarly journals Prevalence of mastitis pathogens in milk from clinically healthy cows

2013 ◽  
Vol 58 (No. 11) ◽  
pp. 567-575 ◽  
Author(s):  
D. Cervinkova ◽  
H. Vlkova ◽  
I. Borodacova ◽  
J. Makovcova ◽  
V. Babak ◽  
...  
Keyword(s):  
Biofilm Formation ◽  
Cow Milk ◽  
Pfge Analysis ◽  
Candida Spp ◽  
Coagulase Negative Staphylococci ◽  
Ampicillin Resistance ◽  
Intramammary Infections ◽  
Milk Samples ◽  
Clonal Spread ◽  
Mastitis Pathogens

A total of 669 individual cow milk samples originating from asymptomatic cows from 16 dairy farms were examined for the presence of microorganisms with the potential to cause mastitis. Coagulase-negative staphylococci clearly predominated (53.5% positive samples) followed by streptococci and enterococci (both occurring in 16.1% samples). Among streptococci, so-called mastitis streptococci (S. uberis, S. dysgalactiae and S. agalactiae) prevailed (11.7% positive samples). Enterobacteriaceae were found in 10.0% samples, most of which (6.6% samples) were positive for Escherichia coli. Yeasts (mainly Candida spp.) were found in 8.2% samples. One of the major mastitis pathogens, Staphylococcus aureus subsp. aureus, was isolated from 9.0% of samples. S. aureus isolates were further characterised in terms of their capability to form biofilm, antimicrobial susceptibility and clonality (PFGE). All S. aureus isolates were capable of biofilm formation and were generally susceptible to the majority of tested antibiotics. The exception was ampicillin, resistance to which was observed in 27.7% isolates. Therefore, the relatively frequent occurrence of S. aureus could be attributed to persistent intramammary infections due to biofilm formation rather than low efficacy of particular antibiotics. PFGE analysis revealed clonal spread of certain S. aureus isolates within and between farms indicating that certain lineages of S. aureus mastitis strains are particularly successful.

A Practical Evaluation of the Delvotest P Multi Plate Test in Screening Raw Milk for Antibiotics

1986 ◽  
Vol 49 (11) ◽  
pp. 868-870 ◽  
Author(s):  
LYSE LAROCQUE ◽  
G. A. NEVILLE
Keyword(s):  
Bacillus Subtilis ◽  
Staphylococcus Epidermidis ◽  
Raw Milk ◽  
The Other ◽  
Penicillin G ◽  
Antibiotic Residues ◽  
Plate Test ◽  
Milk Samples ◽  
Practical Evaluation ◽  
Bacillus Subtilis Atcc

The Delvotest P Multi plate test was evaluated by screening 100 milk samples for total antibiotic residues (penicillin G, streptomycin and neomycin). The samples were taken in conjunction with an antibiotic depletion study in milk derived from six cows treated with a multiple antibiotic, intramammary infusion product. Within the limits of sensitivity of the Delvotest, only penicillin G persisted in milk samples taken beyond 60 h, whereas in some samples, the other antibiotics appeared to be depleted as early as 48 h. More sensitive tests, however, detected neomycin (Staphylococcus epidermidis, ATCC 12228) in 50% of samples taken at 60 h and streptomycin (Bacillus subtilis, ATCC 6633) at 14.5 d after discontinuation of infusion.

Effect of coagulase-negative staphylococci on somatic cell count in Dutch dairy herds

2010 ◽  
Vol 77 (3) ◽  
pp. 318-324 ◽  
Author(s):  
Otlis Sampimon ◽  
Bart HP van den Borne ◽  
Inge Santman-Berends ◽  
Herman W Barkema ◽  
Theo Lam
Keyword(s):  
Somatic Cell ◽  
Cell Count ◽  
Somatic Cell Count ◽  
Health Management ◽  
Dairy Farms ◽  
Subclinical Mastitis ◽  
Dairy Herds ◽  
Coagulase Negative Staphylococci ◽  
Intramammary Infections ◽  
Milk Samples

The effect was quantified of coagulase-negative staphylococci (CNS) intramammary infections on quarter- and cow-level somatic cell count (SCC) and on bulk milk somatic cell count (BMSCC) in different BMSCC cohorts in Dutch dairy herds. Two datasets were used for this purpose. In the first dataset, on 49 randomly selected dairy farms a total of 4220 quarter milk samples of 1072 cows were collected of all cows and heifers with a test-day SCC ⩾250 000 and ⩾150 000 cells/ml, respectively, and of 25% of cows and heifers below these thresholds. In the second dataset, on 39 selected dairy farms a total of 8329 quarter milk samples of 2115 cows were collected of all cows with a test-day SCC ⩾250 000 cells/ml following two consecutive SCC <250 000 cells/ml, and of heifers using the same SCC criteria but with a threshold of 150 000 cells/ml. These cows and heifers were defined as new high SCC. In both datasets, CNS was the most frequently isolated pathogen, 11% in the first dataset and 12% in the second dataset. In both datasets, quarters with CNS IMI had a lower SCC than quarters infected with major pathogens, and a higher SCC than culture-negative quarters. The same was found for SCC at cow level. Coagulase-negative staphylococci were more often found in quarters with SCC ⩾200 000 cells/ml in dairy farms with a BMSCC <150 000 cells/ml compared with dairy farms with a higher BMSCC. Prevalence of CNS in cows and heifers with a high SCC was higher in dairy farms with a BMSCC <150 000 cells/ml compared with dairy farms with a medium or high BMSCC: 30, 19 and 18%, respectively. This indicates that CNS IMI as a cause of subclinical mastitis is relatively more important in dairy farms with a low BMSCC and may become a point of attention in udder health management on that type of farm.

scholarly journals The Impact of Mastitis on Reproductive Parameters of Dairy Cows

2019 ◽  
Vol 63 (3) ◽  
pp. 72-78
Author(s):  
F. Zigo ◽  
A. Adamová ◽  
M. Vasiľ ◽  
J. Elečko ◽  
S. Ondrašovičová ◽  
...  
Keyword(s):  
Dairy Cows ◽  
Subclinical Mastitis ◽  
Clinical Mastitis ◽  
Reproductive Parameters ◽  
Coagulase Negative Staphylococci ◽  
Milk Samples ◽  
California Mastitis Test ◽  
Service Period ◽  
High Incidence ◽  
The Impact

Abstract The aim of this study was to evaluate the occurrence of mastitis and its impact on the reproductive parameters in a herd of 180 dairy cows. Based on the herd records 127 cows of Slovak spotted cattle and their crosses with red Holstein were selected for study between 1—2 months after calving. The examination of the health status of the mammary glands consisted of: the clinical examination of the udder, the California mastitis test (CMT) supplemented by the collection of mixed milk samples, and the laboratory examination of bacterial pathogens causing the mastitis. In addition to the mam­mary investigation, reproduction indicators such as the length of the insemination interval, the service period, the intercalving period and the insemination index were also analyzed. The results of this study indicated: a high incidence of mastitis (41.6 %), especially latent (21.2 %), subclinical (15.7 %) and clinical (4.7 %) forms were most common in the herd. The most frequently isolated bacteria from the infected milk samples were: coagulase negative staphylococci (54.1 %), S. aureus (16.9 %), Streptococcus spp. (15.0 %), A. viridans (7.5 %) and Ent. faecalis (6.4 %). According to the available literature, the optimum values of the intercalving period were 365—400 days, the insemination interval 55—80 days, the insemination index 1.2—2 and the service period 60—110 days. In comparison, our results showed increased, unsatisfactory reproductive values in the group of dairy cows with clinical mastitis. While in healthy cows as well as in groups of cows with latent and subclinical mastitis, all of the reproductive indicators were within the optimal levels.

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