scholarly journals Melanogenesis inhibitory activity of Korean Undaria pinnatifida in mouse B16 melanoma cells

2014 ◽  
Vol 7 (2) ◽  
pp. 89-92 ◽  
Author(s):  
Min-Jin Kim ◽  
Dong Sam Kim ◽  
Hun-Seok Yoon ◽  
Wook Jae Lee ◽  
Nam Ho Lee ◽  
...  
Keyword(s):  
Undaria Pinnatifida ◽  
Traditional Food ◽  
Dependent Manner ◽  
Asian Countries ◽  
Melanin Content ◽  
Traditional Foods ◽  
Whitening Agent ◽  
Few Data ◽  
Dose Dependent ◽  
Different Parts

ABSTRACT A number of seaweed species are used as traditional foods and medicine in different parts of the world, including Asian countries. However, very few data on the anti-melanogenic effect of seaweed have been published. Undaria pinnatifida (Dolmiyeok), a brown alga, is a traditional food in Jeju Island, the southern regions of the Korea peninsula. In this study, ethylacetate extracts of U. pinnatifida (UPE) were examined for their anti-melanogenic potentials. Our results supports the finding that UPE down-regulated melanin content in a dose-dependent pattern. To clarify the target of UPE action in melanogenesis, we performed Western blotting for tyrosinase and microphthalmia-associated transcription factor (MITF), which are key melanogenic enzymes. UPE inhibited tyrosinase and MITF expressions in a dose-dependent manner. These results indicate that treatment with UPE significantly inhibits the melanogenesis in B16 cells, and may be effective in the whitening agent for the skin

scholarly journals Anticoagulant Activity of Polyphenolic-Polysaccharides Isolated fromMelastoma malabathricumL.

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Li Teng Khoo ◽  
Faridah Abas ◽  
Janna Ong Abdullah ◽  
Eusni Rahayu Mohd Tohit ◽  
Muhajir Hamid
Keyword(s):  
Solid Phase ◽  
Chemical Constituents ◽  
Anticoagulant Activity ◽  
Hot Water ◽  
Neutral Sugar ◽  
Dependent Manner ◽  
Asian Countries ◽  
Melastoma Malabathricum ◽  
Solid Phase Extraction Cartridge ◽  
Dose Dependent

Melastoma malabathricumLinn. is a perennial traditional medicine plants that grows abundantly throughout Asian countries. In this study,M. malabathricumLinn. leaf hot water crude extract with anticoagulant activity was purified through solid phase extraction cartridge and examined for the bioactive chemical constituents on blood coagulation reaction. The SPE purified fractions were, respectively, designated as F1, F2, F3, and F4, and each was subjected to the activated partial thromboplastin time (APTT) anticoagulant assay. Active anticoagulant fractions (F1, F2, and F3) were subjected to chemical characterisation evaluation. Besides, neutral sugar for carbohydrate part was also examined. F1, F2, and F3 were found to significantly prolong the anticoagulant activities in the following order,F1>F2>F3, in a dose dependent manner. In addition, carbohydrate, hexuronic acid, and polyphenolic moiety were measured for the active anticoagulant fractions (F1, F2, and F3). The characterisation of chemical constituents revealed that all these three fractions contained acidic polysaccharides (rhamnogalacturonan, homogalacturonan, and rhamnose hexose-pectic type polysaccharide) and polyphenolics. Hence, it was concluded that the presence of high hexuronic acids and polysaccharides, as well as polyphenolics in traditional medicinal plant,M. malabathricum, played a role in prolonging blood clotting in the intrinsic pathway.

scholarly journals Inhibitory Effect and Mechanism of Scutellarein on Melanogenesis

Cosmetics ◽  
2021 ◽  
Vol 8 (1) ◽  
pp. 15
Author(s):  
Liyun Dai ◽  
Lihao Gu ◽  
Kazuhisa Maeda
Keyword(s):  
Chemical Structure ◽  
Inhibitory Effect ◽  
Mrna Levels ◽  
Dependent Manner ◽  
Asian Countries ◽  
Melanin Production ◽  
Free Hydroxyl ◽  
High Concentrations ◽  
Skin Lightening ◽  
Dose Dependent

Fairer skin is preferred in many Asian countries and there is a high demand for skin whitening and lightening products. However, in recent years, problems related to the safety of using whitening agents have emerged. This study demonstrates that plant-derived scutellarein effectively inhibits melanogenesis in B16 melanoma cells. However, baicalein, which is similar to scutellarein in its chemical structure, does not show any inhibitory effect on melanogenesis. Cellular tyrosinase activity is decreased by scutellarein in a dose-dependent manner. No cytotoxicity is observed at the effective concentration range. Additionally, both the protein and mRNA levels of tyrosinase are significantly decreased by scutellarein. Further, the risk of leukoderma development also is determined by evaluating the production of free hydroxyl radicals (˙OH); scutellarein treatment does not induce ˙OH production. Scutellarein shows no risk of causing leukoderma. Our results suggest that scutellarein or plant extracts containing high concentrations of scutellarein have the potential to inhibit melanin production and serve as cosmetic skin-lightening agents.

scholarly journals Induction of Melanogenesis by Fosfomycin in B16F10 Cells Through the Upregulation of P-JNK and P-p38 Signaling Pathways

Antibiotics ◽  
2020 ◽  
Vol 9 (4) ◽  
pp. 172
Author(s):  
Sana Ullah ◽  
You Chul Chung ◽  
Chang-Gu Hyun
Keyword(s):  
Western Blotting ◽  
Disodium Salt ◽  
Water Soluble ◽  
Dependent Manner ◽  
Related Protein ◽  
Melanin Content ◽  
B16f10 Cells ◽  
Tract Infections ◽  
Dose Dependent ◽  
Tyrosinase Related Protein

Fosfomycin disodium salt (FDS), which is a water-soluble extract, is a bactericidal drug used to inhibit the synthesis of cells. Moreover, it has been found to be effective in the treatment of urinary tract infections. The present study was conducted to investigate the melanogenesis-stimulating effect of FDS in B16F10 cells. Several experiments were performed on B16F10 cells: the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay, the melanin content assay, the cellular tyrosinase activity assay, and Western blotting. FDS upregulated the activity of tyrosinase in a dose-dependent manner at a wide concentration range of 0–1 mg/mL, which showed no cytotoxicity. It also increased the melanin content and the activity of the microphthalmia-associated transcription factor (MITF), tyrosinase related protein 1 (TRP-1), and tyrosinase related protein 2 (TRP-2) enzymes in a dose-dependent manner. Western blotting results showed that FDS clearly upregulated the phosphorylation of c-Jun N-terminal kinases (JNK) and p38 pathways. These data are clear evidence of the melanogenesis-inducing effect of FDS in B16F10 murine melanoma cells.

INHIBITION OF DIPHOSPHONATE-BLOCKED BONE MINERALIZATION. EVIDENCE THAT CALCITONIN PROMOTES MINERALIZATION

1979 ◽  
Vol 91 (2) ◽  
pp. 351-361 ◽  
Author(s):  
Alfred Boris ◽  
James F. Hurley ◽  
Thelma Trmal ◽  
John P. Mallon ◽  
Diana S. Matuszewski
Keyword(s):  
Bone Mineral ◽  
Bone Mineralization ◽  
Dry Weight ◽  
Dependent Manner ◽  
Bone Ash ◽  
Few Data ◽  
Mineral Resorption ◽  
Tibial Epiphyseal ◽  
Dose Dependent ◽  
Epiphyseal Plates

ABSTRACT There is much experimental evidence which indicates that calcitonin inhibits bone mineral resorption, but there are few data available in support of the proposal that calcitonin may also promote mineralization. Ethane-1-hydroxy-1,1-diphosphonate (EHDP) administered to immature rats inhibited mineralization as evidenced by widened tibial epiphyseal plates and decreased bone ash to dry weight ratios. Concurrent dosing with salmon calcitonin (SCT) prevented or reversed the EHDP-blocked mineralization in a dose dependent manner. Administration of SCT during the period after EHDP treatment significantly improved mineralization of tibial epiphyseal plates as shown by plate width narrowing and increased uptake of radioactive calcium. These results suggest that SCT increased mineralization in EHDP-treated rats, and provide supportive evidence for the proposal that calcitonin may also promote mineralization, in addition to its well known ability to inhibit bone mineral resorption.

Differential Neuritogenic Activities of Two Edible Brown Macroalgae, Undaria pinnatifida and Saccharina japonica

2014 ◽  
Vol 42 (06) ◽  
pp. 1371-1384 ◽  
Author(s):  
Md. Abdul Hannan ◽  
Md. Mohibbullah ◽  
Seon-Yeong Hwang ◽  
Kyungyong Lee ◽  
Yang-Chun Kim ◽  
...  
Keyword(s):  
Hippocampal Neurons ◽  
Undaria Pinnatifida ◽  
Saccharina Japonica ◽  
Dependent Manner ◽  
Brown Macroalgae ◽  
Neuroprotective Effects ◽  
Beneficial Effects ◽  
Ethanol Extracts ◽  
Dose Dependent

Undaria pinnatifida (Harvey) Suringar and Saccharina japonica Areschoug are two common seaweeds, and both are known to have numerous pharmacological properties that include neuroprotective effects. In a previous study, we found that the ethanol extracts of U. pinnatifida (UPE) and S. japonica (SJE) had neurite promoting activities on developing hippocampal neurons. In the present study, we studied and compared the effects of UPE and SJE on neuronal maturation. Both UPE and SJE promoted neurite outgrowth in a dose-dependent manner with optimal concentrations of 5 and 15 μg/mL, respectively. Initial neuronal differentiation was significantly promoted by UPE and SJE. Subsequently, treatment with both increased indices of axonal and dendritic cytoarchitecture, such as, the numbers and lengths of primary processes, although only UPE had a significant effect on branching frequencies. In addition, UPE and SJE showed no evidence of cytotoxicity, rather they protected neurons from naturally occurring death in vitro. These results indicate that UPE and SJE promote axodendritic maturation and neuronal survival and suggest that these algal extracts, especially UPE, have beneficial effects on the nervous system.

scholarly journals Effect of Purified Mushroom Tyrosinase on Melanin Content and Melanogenic Protein Expression

2016 ◽  
Vol 2016 ◽  
pp. 1-8 ◽  
Author(s):  
Kamal Uddin Zaidi ◽  
Sharique A. Ali ◽  
Ayesha S. Ali
Keyword(s):  
Kinase Inhibitors ◽  
Pigment Cell ◽  
Adenosine Monophosphate ◽  
Camp Response Element Binding ◽  
Protein Kinase Inhibitors ◽  
Tyrosinase Activity ◽  
Dependent Manner ◽  
Mushroom Tyrosinase ◽  
Melanin Content ◽  
Dose Dependent

In mammalian melanocytes, melanosome is a highly specialized organelle where melanin is synthesized. Melanin synthesis is controlled by tyrosinase, the vital enzyme in melanogenic pathway. The present investigation is based on an effect of purified mushroom tyrosinase of Agaricus bisporus on B16F10 melanocytes for the melanin production via blocking pigment cell machinery. Using B16F10 melanocytes showed that the stimulation of melanogenesis by purified tyrosinase is due to increased tyrosinase absorption. Cellular tyrosinase activity and melanin content in B16F10 melanocytes were increased by purified tyrosinase in a dose-dependent manner. Western blot analysis revealed that cellular tyrosinase levels were enhanced after treatment with purified tyrosinase for 48 hours. Furthermore, tyrosinase induced phosphorylation of cyclic adenosine monophosphate (cAMP) response element-binding protein (CREB) in a dose-dependent manner. The purified tyrosinase-mediated increase of tyrosinase activity was significantly attenuated by H89, LY294002, Ro-32-0432, and PD98059, cAMP-dependent protein kinase inhibitors. The results indicate that purified tyrosinase can be used as contestant for the treatment of vitiligous skin conditions.

scholarly journals Anti-Melanogenic Effect of Chestnut Spike Extract through Downregulation of Tyrosinase-Related Proteins and Activation of ERK 1/2

2018 ◽  
Vol 13 (8) ◽  
pp. 1934578X1801300
Author(s):  
Jung-Hee Byeon ◽  
Md Badrul Alam ◽  
Ki-Chan Kim ◽  
Sangsun Heo ◽  
Ji-young Lim ◽  
...  
Keyword(s):  
Specific Inhibitor ◽  
Phenotypic Trait ◽  
Dependent Manner ◽  
Melanin Content ◽  
Extracellular Signal Regulated Kinase ◽  
Extracellular Signal ◽  
Key Factor ◽  
Related Proteins ◽  
Dose Dependent ◽  
Mitf Expression

Melanin has been reported to be the key factor for skin homeostasis. Besides defining an important human phenotypic trait, melanin overproduction may cause various disorders such as vitiligo, Addison's disease, Cushing's syndrome, and melasma. In this study, we aimed to investigate the anti-melanogenic potential of dried spike extract of chestnut. The extract inhibited tyrosinase (TYR) activity in a dose-dependent manner. Cellular melanin content decreased markedly after treatment with the extract. The spike extract inhibited microphthalmia-associated transcription factor (MITF) expression and downregulated TYR, TYRP-1, and TYRP-2 protein expression by increasing the phosphorylation of extracellular signal-regulated kinase (ERK)1/2 signalling pathway in melan-a cells. In addition, treatment with U0126, a specific inhibitor of ERK, restored melanin content. Collectively, these results suggest that the chestnut spike extract attenuated melanogenesis by inhibiting MITF expression and downregulating TYR, TYRP-1, and TYRP-2 protein expressions via activation of ERK1/2 pathway.

Anisaldehyde, a Melanogenesis Potentiator

2007 ◽  
Vol 62 (1-2) ◽  
pp. 143-149 ◽  
Author(s):  
Teruhiko Nitoda ◽  
Maria D. Fan ◽  
Isao Kubo
Keyword(s):  
Adverse Effect ◽  
Melanoma Cells ◽  
Dependent Manner ◽  
Tyrosinase Inhibitor ◽  
Melanin Content ◽  
Dose Dependent ◽  
A375 Melanoma

Anisaldehyde (4-methoxybenzaldehyde), previously reported as a tyrosinase inhibitor, did not inhibit melanogenesis in cultured B16-F10 melanoma cells but rather enhanced it. This adverse effect of anisaldehyde was accompanied by melanocytotoxicity in a dose-dependent manner up to 2 mм. The melanin content per cell at 1 mм was increased 5-fold compared to control and morphological observations showed the deposition of melanin pigments. Anisaldehyde was also examined against cultured human A375 melanoma cells.

scholarly journals Phenolic Characterization and Neuroprotective Properties of Grape Pomace Extracts

Molecules ◽  
2021 ◽  
Vol 26 (20) ◽  
pp. 6216
Author(s):  
Annalisa Chiavaroli ◽  
Marwa Balaha ◽  
Alessandra Acquaviva ◽  
Claudio Ferrante ◽  
Amelia Cataldi ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Water Extract ◽  
Grape Pomace ◽  
Dependent Manner ◽  
Rt Pcr ◽  
Pge2 Release ◽  
Cox 2 ◽  
Dose Dependent ◽  
Different Parts

Vitis vinifera (grape) contains various compounds with acknowledged phytochemical and pharmacological properties. Among the different parts of the plant, pomace is of particular interest as a winemaking industry by-product. A characterization of the water extract from grape pomace from Montepulciano d’Abruzzo variety (Villamagna doc) was conducted, and the bioactive phenolic compounds were quantified through HPLC-DAD-MS analysis. HypoE22, a hypothalamic cell line, was challenged with an oxidative stimulus and exposed to different concentrations (1 µg/mL–1 mg/mL) of the pomace extract for 24, 48, and 72 h. In the same conditions, cells were exposed to the sole catechin, in a concentration range (5–500 ng/mL) consistent with the catechin level in the extract. Cell proliferation was investigated by MTT assay, dopamine release through HPLC-EC method, PGE2 amount by an ELISA kit, and expressions of neurotrophin brain-derived neurotrophic factor (BDNF) and of cyclooxygenase-2 (COX-2) by RT-PCR. The extract reverted the cytotoxicity exerted by the oxidative stimulus at all the experimental times in a dose-dependent manner, whereas the catechin was able to revert the oxidative stress-induced depletion of dopamine 48 h and 72 h after the stimulus. The extract and the catechin were also effective in preventing the downregulation of BDNF and the concomitant upregulation of COX-2 gene expression. In accordance, PGE2 release was augmented by the oxidative stress conditions and reverted by the administration of the water extract from grace pomace and catechin, which were equally effective. These results suggest that the neuroprotection induced by the extract could be ascribed, albeit partially, to its catechin content.

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