scholarly journals ANALYTICAL AND CLINICAL EVALUATION OF SYSMEX UF1000I FOR AUTOMATED SCREENING OF CEREBROSPINAL FLUIDS ANALITIČKA I KLINIČKA EVALUACIJA UREĐAJA SYSMEX UF1000I ZA AUTOMATSKI SKRINING CEREBROSPINALNIH TEČNOSTI

2013 ◽  
Vol 33 (2) ◽  
pp. 191-196 ◽  
Author(s):  
Sabrina Buoro ◽  
Cosimo Ottomano ◽  
Sara Appassiti Esposito ◽  
Patrizia Gherardi ◽  
Maria Grazia Alessio ◽  
...  
Keyword(s):  
Optical Microscopy ◽  
Microscopic Analysis ◽  
Turnaround Time ◽  
Cell Counting ◽  
Rapid Screening ◽  
Effi Ciency ◽  
Sensitivity Tests ◽  
Leukocyte Counts ◽  
Automated Screening ◽  
Carry Over

Summary Background: We evaluated the performance of Sysmex UF- 1000i for cell counting and differential cell count, as well as for assessment of bacteria load in cerebrospinal fluid (CSF), as a potential approach for the rapid screening of meningitis or bacterial encephalitis. Methods: We analyzed 77 consecutive CSF samples, 34 of which (44%) displayed leukocyte count >5 white blood cell (WBQ/^iL with optical microscopy. Results on the UF-1000i were compared with those obtained by microscopic analysis. Imprecision was evaluated by testing three CSF samples with leukocyte values between 3.5 and 28.8 WBC/nL in 10 repli- cates. Carry-over was evaluated with the Broughton formula on three CSF pools with leukocyte counts between 93.5 and 132.5 WBC/^L. Linearity was assessed according to CLSI document EP6-A. In the presence of bacteria, identification and antibiogram were performed with Vitex (Biomerieux), except for Neisserie meningitidis (ApiNH, Biomerieux). Sensitivity tests were performed with Vitex and disc diffusion. Results: Optimal correlation was found between UF-1000i and optical microscopy, displaying Pearson's correlation of 0.99 and mean bias of-3.5 WBC/^L (95% Cl, from -7.0 to 0.0 WBC/nL). Imprecision varied between 12 and 16%. Li- nearity was excellent, 4-278 WBC/nL. Carry-over was neg- ligible. ROC analysis yielded AUC of 0.99 for both WBC and bacterial counts. The agreement at threshold >4 WBC/nL was 0.91, with sensitivity and specificity of 1.00 and 0.84. At S19 bacteria^nL cut-off, accuracy was 0.98, sensitivity 1.00 and specificity 0.97. Conclusions: According to these results, CSF screening with UF-I000i seems a reliable approach in terms of instrument performance, turnaround time and overall laboratory effi- ciency.

scholarly journals Automated cell count in body fluids: a review

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
María José Alcaide Martín ◽  
Laura Altimira Queral ◽  
Laura Sahuquillo Frías ◽  
Laura Valiña Amado ◽  
Anna Merino ◽  
...  
Keyword(s):  
Cell Count ◽  
Optical Microscopy ◽  
Turnaround Time ◽  
Body Fluids ◽  
Cell Counting ◽  
Highly Qualified ◽  
Gold Standard Method ◽  
Automated Cell Counting ◽  
Fluid Cell

Abstract Body fluid cell counting provides valuable information for the diagnosis and treatment of a variety of conditions. Chamber cell count and cellularity analysis by optical microscopy are considered the gold-standard method for cell counting. However, this method has a long turnaround time and limited reproducibility, and requires highly-trained personnel. In the recent decades, specific modes have been developed for the analysis of body fluids. These modes, which perform automated cell counting, are incorporated into hemocytometers and urine analyzers. These innovations have been rapidly incorporated into routine laboratory practice. At present, a variety of analyzers are available that enable automated cell counting for body fluids. Nevertheless, these analyzers have some limitations and can only be operated by highly-qualified laboratory professionals. In this review, we provide an overview of the most relevant automated cell counters currently available for body fluids, the interpretation of the parameters measured by these analyzers, their main analytical features, and the role of optical microscopy as automated cell counters gain ground.

Evaluation of H-800/FUS-100 automatic urine analyzer performance

2017 ◽  
Vol 43 (1) ◽  
pp. 89-92
Author(s):  
Müjgan Ercan ◽  
Esra Fırat Oğuz ◽  
Oğuzhan Kaya ◽  
Fatma Meriç Yılmaz
Keyword(s):  
Chemical Analysis ◽  
Calcium Oxalate ◽  
Urine Analysis ◽  
Microscopic Analysis ◽  
Automated Systems ◽  
Urine Sediment ◽  
Error Sources ◽  
Leukocyte Counts ◽  
Carry Over ◽  
Fresh Urine

AbstractObjective:Automated urine analysis is usually preferred for laboratories with intensive workload. The aim of this study was to evaluate the performance of the automated urine analyser H-800/FUS-100 and detect the error sources.Materials and methods:One thousand four hundred fifty nine fresh urine samples were analyzed with H-800/FUS-100 automated systems. The urine sediment of the samples with discrepant strip and microscopy results were confirmed by manual microscopy. Precision and carry over studies were performed.Results:The discrepancy is detected in a ratio of 5.89% between chemical analysis (H-800) and microscopic analysis (FUS-100) of the device. A total of 86 discrepant samples were detected. Fifty six of 86 were erythrocyte discrepancies (65.1%) and 30 of 86 were leukocyte discrepancies (34.9%). The results of carry over analysis for erythrocyte and leukocyte were 21.85% and 13.64%, respectively.Conclusions:Sixteen (1.09%) of 1459 patients’ results in FUS-100 were discrepant with manual microscopy. Commonly, yeasts and crystals affected erythrocyte counts and calcium oxalate and amorphous crystals affected the leukocyte counts. Images should be reviewed for every sample when automated systems are used for urine analysis. Especially if discrepancy is detected between chemical and microscobic analysis, the results should also be confirmed with manual microscopy.

scholarly journals Development and analytical performance of an automated screening method for cannabinoids on the Dimension clinical chemistry system

1997 ◽  
Vol 19 (5) ◽  
pp. 169-173 ◽  
Author(s):  
D. M. Obzansky ◽  
E. G. Gorman ◽  
S. P. Kramer ◽  
I. S. Masulli ◽  
E. A. Nuzzaci ◽  
...  
Keyword(s):  
Carboxylic Acid ◽  
Clinical Chemistry ◽  
Screening Method ◽  
Random Access ◽  
Analytical Performance ◽  
Access Method ◽  
Extensive Evaluation ◽  
Automated Screening ◽  
Carry Over

A fully automated, random access method for the determination of cannabinoids (UTHC) was developed for the Dimension AR and XL clinical chemistry systems. The method utilizes Abuscreen ONLINE reagents and a multianalyte liquid calibrator containing 11-nor-Δ9-THC-9-carboxylic acid. Within-run and total reproducibility, determined using NCCLS protocol EP5- T2, was less than 0.6% and 1.6% CV, respectively, at all concentrations. Calibration stability was retained for at least 30 days. An extensive evaluation of non-structurally related drugs and various physiological substances indicated lack of interference in the method. No sample carry-over was observed following a specimen containing 1886 ng/ml 11-nor-Δ9-THC-9-carboxylic acid. A 99.1% agreement (N = 445 samples) was found between an EMIT based method on the aca discrete clinical analyser and the Dimension UTHC method.Dimension clinical chemistry system and aca discrete clinical analyser are registered trademarks of Dade International.

scholarly journals Evaluation of Cyclosaplin Efficacy Using a Silk Based 3D Tumor Model

Biomolecules ◽  
2019 ◽  
Vol 9 (4) ◽  
pp. 123 ◽  
Author(s):  
Abheepsa Mishra ◽  
Sourav K Mukhopadhyay ◽  
Satyahari Dey
Keyword(s):  
Breast Cancer ◽  
Cell Mass ◽  
3D Culture ◽  
Microscopic Analysis ◽  
Tumor Model ◽  
Rapid Screening ◽  
Screening Process ◽  
3D Microenvironment ◽  
Anti Cancer

Development of novel anti-cancer peptides requires a rapid screening process which can be accelerated by using appropriate in vitro tumor models. Breast carcinoma tissue is a three-dimensional (3D) microenvironment, which contains a hypoxic center surrounded by dense proliferative tissue. Biochemical clues provided by such a 3D cell mass cannot be recapitulated in conventional 2D culture systems. In this experiment, we evaluate the efficacy of the sandalwood peptide, cyclosaplin, on an established in vitro 3D silk breast cancer model using the invasive MDA-MB-231 cell line. The anti-proliferative effect of the peptide on the 3D silk tumor model is monitored by alamarBlue assay, with conventional 2D culture as control. The proliferation rate, glucose consumed, lactate dehydrogenase (LDH), and matrix metalloproteinase 9 (MMP-9) activity of human breast cancer cells are higher in 3D constructs compared to 2D. A higher concentration of drug is required to achieve 50% cell death in 3D culture than in 2D culture. The cyclosaplin treated MDA-MB-231 cells showed a significant decrease in MMP-9 activity in 3D constructs. Microscopic analysis revealed the formation of cell clusters evenly distributed in the scaffolds. The drug treated cells were less in number, smaller and showed unusual morphology. Overall, these findings indicate the role of cyclosaplin as a promising anti-cancer therapeutic.

scholarly journals MODULARANALYTICS: A New Approach to Automation in the Clinical Laboratory

2005 ◽  
Vol 2005 (1) ◽  
pp. 8-25 ◽  
Author(s):  
Gary L. Horowitz ◽  
Zahur Zaman ◽  
Norbert J. C. Blanckaert ◽  
Daniel W. Chan ◽  
Jeffrey A. Dubois ◽  
...  
Keyword(s):  
Clinical Laboratory ◽  
Clinical Chemistry ◽  
Control Unit ◽  
Turnaround Time ◽  
Chemistry Laboratory ◽  
New Approach ◽  
Typical Sample ◽  
Clinical Chemistry Laboratory ◽  
Carry Over ◽  
Roche Diagnostics

MODULARANALYTICS(Roche Diagnostics) (MODULARANALYTICS, Elecsys and Cobas Integra are trademarks of a member of the Roche Group) represents a new approach to automation for the clinical chemistry laboratory. It consists of a control unit, a core unit with a bidirectional multitrack rack transportation system, and three distinct kinds of analytical modules: an ISE module, a P800 module (44 photometric tests, throughput of up to 800 tests/h), and a D2400 module (16 photometric tests, throughput up to 2400 tests/h). MODULARANALYTICSallows customised configurations for various laboratory workloads. The performance and practicability of MODULARANALYTICSwere evaluated in an international multicentre study at 16 sites. Studies included precision, accuracy, analytical range, carry-over, and workflow assessment. More than 700 000 results were obtained during the course of the study. Median between-day CVs were typically less than 3% for clinical chemistries and less than 6% for homogeneous immunoassays. Median recoveries for nearly all standardised reference materials were within 5% of assigned values. Method comparisons versus current existing routine instrumentation were clinically acceptable in all cases. During the workflow studies, the work from three to four single workstations was transferred to MODULARANALYTICS, which offered over 100 possible methods, with reduction in sample splitting, handling errors, and turnaround time. Typical sample processing time on MODULARANALYTICSwas less than 30 minutes, an improvement from the current laboratory systems. By combining multiple analytic units in flexible ways, MODULARANALYTICSmet diverse laboratory needs and offered improvement in workflow over current laboratory situations. It increased overall efficiency while maintaining (or improving) quality.

N,P-Doped Carbon Nanodots for Food-Matrix Decontamination, Anticancer Potential, and Cellular Bio-Imaging Applications

2020 ◽  
Vol 16 (3) ◽  
pp. 283-303 ◽  
Author(s):  
Vivek K. Bajpai ◽  
Imran Khan ◽  
Shruti Shukla ◽  
Pradeep Kumar ◽  
Lei Chen ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Microscopic Analysis ◽  
Treatment Method ◽  
Solid Substrate ◽  
Bacterial Biofilm ◽  
Cell Counting ◽  
Carbon Nanodots ◽  
Dead Cell ◽  
Food Matrix ◽  
Bio Imaging

We report a facile one-step thermal treatment method for the synthesis of biocompatible, fluorescent nitrogen-phosphorus-doped carbon nanodots (NPCDs) as multifunctional agents for the food matrix decontamination, cancer targeting, and cellular bio-imaging. NPCDs exhibit high toxicity towards L. monocytogenes, as illustrated by fluorescent live-dead cell counting, disruption of membrane permeability/potential, changes in the levels of cellular ions, genetic materials, and proteins, as well as intracellular production of reactive oxygen species. The tryptophan and protein peaks released in NPCDs treated cells contributed to indole ring breathing and correlated with induced cell death. NPCDs significantly inhibited bacterial biofilm formation on a solid substrate. NPCDs-coated low-density polyethylene (LDPE) film crosslinked with 1% aminopropyltriethoxy silane (APTES) via silane-hydroxyl linking as a food-grade wrap significantly reduced bacterial counts in a raw chicken food model. Furthermore, NPCDs induced apoptosis in HeLa cervical cancer cells, as confirmed by the distorted cell morphology, fluorescence microscopic analysis, presence of fragmented nuclei and the qPCR results of mRNA expression levels of apoptotic markers. Moreover, NPCDs were also applicable in utilized for the cellular bio-imaging of KM12-C colon cancer cells under confocal microscopy owing to their excellent luminescence properties. Overall, NPCDs represent a promising platform to reduce the environmental health risks associated with hazardous pathogens, anticancer targeting, and their application in cellular bio-imaging as multifunctional targets/nanocarriers.

Fluoro-opto-electronic cell-counting on milk

1975 ◽  
Vol 42 (2) ◽  
pp. 227-239 ◽  
Author(s):  
P. Schmidt Madsen
Keyword(s):  
Potassium Dichromate ◽  
Coulter Counter ◽  
Cell Counting ◽  
Paper Strip ◽  
Milk Samples ◽  
Digital Counter ◽  
Cell Counts ◽  
Fully Automatic ◽  
Particle Counts ◽  
Carry Over

SummaryThe equipment tested (Fossomatic) is a fully automatic electronic instrument for counting the somatic cells in milk samples. The results are printed on a paper strip and displayed on a digital counter. The capacity is 180 samples/h.Counting results obtained with the instrument have been compared with direct microscopic cell counts and particle counts by Coulter counter. The correlation coefficient with microscopic cell counts was 0·922–0·988, and with counts by the Coulter counter it was 0·916–0·992.Reproducibility showed a coefficient of variation of 2–5 %. The carry-over between the samples was 2·0 %. Preserving milk samples with 0·05 % potassium dichromate did not influence cell counts, whereas freezing the samples reduced the counts 30 %.

scholarly journals An Electronic Blood-Cell-Counting Machine

Blood ◽  
1958 ◽  
Vol 13 (4) ◽  
pp. 398-409 ◽  
Author(s):  
P. CROSLAND-TAYLOR ◽  
J. W. STEWART ◽  
G. HAGGIS
Keyword(s):  
Blood Cell ◽  
Cell Count ◽  
Cell Counting ◽  
Red Cell ◽  
Leukocyte Counts ◽  
Red Cell Count ◽  
Blood Cell Counting

Abstract An electronic blood cell counting machine operating on a flow principle is described. It is quick and easy to use; it can perform a red cell count in ten seconds with an accuracy of ± 2.1 per cent (that is, 5000 cells counted in 10 seconds for a 5 M count). Leukocyte counts can also be performed in ten seconds with an accuracy of ± 9 per cent for a single count (500 cells counted in a 5000 cu.cm. count).

The Effect of Rapid Screening for Methicillin-ResistantStaphylococcus aureus(MRSA) on the Identification and Earlier Isolation of MRSA-Positive Patients

2010 ◽  
Vol 31 (4) ◽  
pp. 374-381 ◽  
Author(s):  
Eilish Creamer ◽  
Anthony Dolan ◽  
Orla Sherlock ◽  
Toney Thomas ◽  
John Walsh ◽  
...  
Keyword(s):  
At Risk ◽  
Predictive Value ◽  
Tertiary Care ◽  
Turnaround Time ◽  
Rapid Screening ◽  
Pcr Assay ◽  
Period 2 ◽  
Before And After ◽  
Patients At Risk ◽  
Risk Patients

Objectives.(1) To determine whether rapid screening with polymerase chain reaction (PCR) assays leads to the earlier isolation of patients at risk for methicillin-resistantStaphylococcus aureus(MRSA) colonization, (2) to assess compliance with routine MRSA screening protocols, (3) to confirm the diagnostic accuracy of the Xpert MRSA real-time PCR assay (Cepheid) by comparison with culture, and (4) to compare turnaround times for PCR assay results with those for culture results.Design.Before-and-after study conducted in a 700-bed acute tertiary care referral hospital. Study periods were (1) a 5-week period before PCR testing began, (2) a 10-week period when the PCR assay was used, and (3) a 5-week period after PCR testing was discontinued.Results.Among 489 at-risk patients, MRSA was isolated from 20 (33%) of 60 patients during period 1, 77 (22%) of 349 patients during period 2, and 18 (23%) of 80 patients during period 3. Twenty-two (27%) of 82 at-risk patients were not screened during period 1, compared with 40 (10%) of 389 at-risk patients not screened during period 2 (P< .001). More MRSA-positive patients were preemptively isolated during periods 1 and 3 compared with period 2 (34 [24%] of 140 vs 28 [8%] of 389;P< .001); however, more MRSA-positive patients were isolated after notification of MRSA-positive results during period 2 (47 [13%] of 349) compared with periods 1 and 3 (2 [1%] of 140;P< .001). The sensitivity, specificity, positive predictive value, and negative predictive value of the PCR assay were 95%, 97%, 82%, and 99%, respectively. The mean turnaround time from receipt of specimens in the laboratory to PCR assay result was 2.6 hours.Conclusions.Rapid screening with the Xpert MRSA PCR assay facilitated compliance with screening policies and the earlier isolation of MRSA-positive Patients. Discrepant results confirm that PCR testing should be used as a screening tool rather than as a diagnostic tool.

scholarly journals Current and emerging colistin resistance diagnostics: a review of established and innovative detection methods

2020 ◽  
Author(s):  
Tumisho Mmatumelo Seipei Leshaba ◽  
Nontombi Marylucy Mbelle ◽  
John Osei Sekyere
Keyword(s):  
Animal Health ◽  
Turnaround Time ◽  
Rapid Screening ◽  
Detection Methods ◽  
Journal Articles ◽  
Colistin Resistance ◽  
Golden Standard ◽  
Phenotypic Methods ◽  
English Articles ◽  
Sensitivity Specificity

Background. The escalation of colistin resistance, due to transferable mcr-genes, threatens public and animal health as there are limited therapeutic options. Given that colistin is a last-line antibiotic, there is a need to contain the spread of its resistance to conserve its efficacy. Herein, current and emerging colistin resistance diagnostics are described to inform faster clinical diagnostic choices. Methods. A literature search in Pubmed, between 2016 and 2020, was performed. English articles evaluating colistin resistance methods/diagnostics were included. Results. Screening resulted in the inclusion of 89 journal articles. Current colistin resistance diagnostics are either phenotypic or molecular. Broth microdilution (BMD) is currently the only golden standard for determining colistin MIC values. However, other methods have been developed to overcome the challenges associated with the BMD. Phenotypic methods comprise agar-based methods i.e. ChromAgar Col-APSE, SuperPolymyxin, ChromID Colistin R etc., MIC-determiners i.e. Microscan, BD Phoenix, Sensitest colistin, Sensititre, Micronaut etc., MCR-detectors i.e. lateral flow immunoassay (LFI), chelator based assays i.e. EDTA- and DPA-based tests as well as biochemical colorimetric tests i.e. Rapid Polymyxin NP test, Rapid ResaPolymyxin NP test etc. Molecular methods only characterize mobile colistin resistance; they include PCR, LAMP, whole-genome sequencing (WGS) etc. Conclusion. Considering the efficiency (sensitivity, specificity, turnaround time) of the Rapid ResaPolymyxin NP test, it is recommended for the initial screening of colistin-resistant isolates. This can be followed by chelator-based tests or the LFI for the rapid screening of mcr-genes. However molecular assays such as LAMP, PCR may be considered for use in well-equipped clinical laboratories.

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