scholarly journals Automated cell count in body fluids: a review

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
María José Alcaide Martín ◽  
Laura Altimira Queral ◽  
Laura Sahuquillo Frías ◽  
Laura Valiña Amado ◽  
Anna Merino ◽  
...  
Keyword(s):  
Cell Count ◽  
Optical Microscopy ◽  
Turnaround Time ◽  
Body Fluids ◽  
Cell Counting ◽  
Highly Qualified ◽  
Gold Standard Method ◽  
Automated Cell Counting ◽  
Fluid Cell

Abstract Body fluid cell counting provides valuable information for the diagnosis and treatment of a variety of conditions. Chamber cell count and cellularity analysis by optical microscopy are considered the gold-standard method for cell counting. However, this method has a long turnaround time and limited reproducibility, and requires highly-trained personnel. In the recent decades, specific modes have been developed for the analysis of body fluids. These modes, which perform automated cell counting, are incorporated into hemocytometers and urine analyzers. These innovations have been rapidly incorporated into routine laboratory practice. At present, a variety of analyzers are available that enable automated cell counting for body fluids. Nevertheless, these analyzers have some limitations and can only be operated by highly-qualified laboratory professionals. In this review, we provide an overview of the most relevant automated cell counters currently available for body fluids, the interpretation of the parameters measured by these analyzers, their main analytical features, and the role of optical microscopy as automated cell counters gain ground.

Performance of Abbott Alinity hq hematology analyzer for automated cell counting of body fluids

2021 ◽  
Author(s):  
Pauline H. Herroelen ◽  
Simke Demeester ◽  
Serge Damiaens ◽  
Anton Evenepoel ◽  
Kristin Jochmans
Keyword(s):  
Body Fluids ◽  
Cell Counting ◽  
Automated Cell Counting ◽  
Hematology Analyzer

scholarly journals ANALYTICAL AND CLINICAL EVALUATION OF SYSMEX UF1000I FOR AUTOMATED SCREENING OF CEREBROSPINAL FLUIDS ANALITIČKA I KLINIČKA EVALUACIJA UREĐAJA SYSMEX UF1000I ZA AUTOMATSKI SKRINING CEREBROSPINALNIH TEČNOSTI

2013 ◽  
Vol 33 (2) ◽  
pp. 191-196 ◽  
Author(s):  
Sabrina Buoro ◽  
Cosimo Ottomano ◽  
Sara Appassiti Esposito ◽  
Patrizia Gherardi ◽  
Maria Grazia Alessio ◽  
...  
Keyword(s):  
Optical Microscopy ◽  
Microscopic Analysis ◽  
Turnaround Time ◽  
Cell Counting ◽  
Rapid Screening ◽  
Effi Ciency ◽  
Sensitivity Tests ◽  
Leukocyte Counts ◽  
Automated Screening ◽  
Carry Over

Summary Background: We evaluated the performance of Sysmex UF- 1000i for cell counting and differential cell count, as well as for assessment of bacteria load in cerebrospinal fluid (CSF), as a potential approach for the rapid screening of meningitis or bacterial encephalitis. Methods: We analyzed 77 consecutive CSF samples, 34 of which (44%) displayed leukocyte count >5 white blood cell (WBQ/^iL with optical microscopy. Results on the UF-1000i were compared with those obtained by microscopic analysis. Imprecision was evaluated by testing three CSF samples with leukocyte values between 3.5 and 28.8 WBC/nL in 10 repli- cates. Carry-over was evaluated with the Broughton formula on three CSF pools with leukocyte counts between 93.5 and 132.5 WBC/^L. Linearity was assessed according to CLSI document EP6-A. In the presence of bacteria, identification and antibiogram were performed with Vitex (Biomerieux), except for Neisserie meningitidis (ApiNH, Biomerieux). Sensitivity tests were performed with Vitex and disc diffusion. Results: Optimal correlation was found between UF-1000i and optical microscopy, displaying Pearson's correlation of 0.99 and mean bias of-3.5 WBC/^L (95% Cl, from -7.0 to 0.0 WBC/nL). Imprecision varied between 12 and 16%. Li- nearity was excellent, 4-278 WBC/nL. Carry-over was neg- ligible. ROC analysis yielded AUC of 0.99 for both WBC and bacterial counts. The agreement at threshold >4 WBC/nL was 0.91, with sensitivity and specificity of 1.00 and 0.84. At S19 bacteria^nL cut-off, accuracy was 0.98, sensitivity 1.00 and specificity 0.97. Conclusions: According to these results, CSF screening with UF-I000i seems a reliable approach in terms of instrument performance, turnaround time and overall laboratory effi- ciency.

The role of technology in haematology

2016 ◽  
Author(s):  
Shaun R. McCann
Keyword(s):  
Blood Cells ◽  
Cell Counting ◽  
Direct Result ◽  
Technological Advances ◽  
Automated Cell Counting ◽  
Technological Developments ◽  
Staining Techniques ◽  
Haematological Disorders ◽  
Confocal And Electron Microscopy

Various technologies have changed and improved the practice of haematology. Because it is possible to obtain blood cells so readily (via a simple venepuncture), haematology has been at the forefront of technological developments in medicine. The diagnosis of both malignant and benign haematological disorders has become more exact because of the technological advances outlined, and the understanding of the pathogenesis of many diseases has been advanced as a direct result of the application of technologies such as immunofluorescence, confocal and electron microscopy, automated cell counting, flow cytometry, digital cell morphology, advanced staining techniques, and PCR. However, it is important to stress that all technologies and ‘tests’ need to be cautiously interpreted, and a full history and physical examination should always be the first step in the investigation of patients.

Comparison between optical microscopy and automation for cytometric analysis of pericardial fluids in a cohort of adult subjects undergoing cardiac surgery

2019 ◽  
Vol 72 (7) ◽  
pp. 493-500 ◽  
Author(s):  
Sabrina Buoro ◽  
Michela Seghezzi ◽  
Maria del Carmen Baigorria Vaca ◽  
Barbara Manenti ◽  
Valentina Moioli ◽  
...  
Keyword(s):  
Optical Microscopy ◽  
Limit Of Detection ◽  
Pericardial Fluid ◽  
Cell Counting ◽  
Current Evidence ◽  
Limited Information ◽  
Fluid Analysis ◽  
Automated Cell Counting ◽  
Limit Of Quantitation ◽  
Number Of Cells

AimsLimited information is available on number and type of cells present in the pericardial fluid (PF). Current evidence and has been garnered with inaccurate application of guidelines for analysis of body fluids. This study was aimed at investigating the performance of automate cytometric analysis of PF in adult subjects.MethodsSeventy-four consecutive PF samples were analysed with Sysmex XN with a module for body fluid analysis (XN-BF) and optical microscopy (OM). The study also encompassed the assessment of limit of blank, limit of detection and limit of quantitation (LoQ), imprecision, carryover and linearity of XN-BF module.ResultsXN-BF parameters were compared with OM for the following cell classes: total cells (TC), leucocytes (white blood cell [WBC]), polymorphonuclear (PMN) and mononuclear (MN) cells. The relative bias were −4.5%, 71.2%, 108.2% and −47.7%, respectively. Passing and Bablok regression yielded slope comprised between 0.06 for MN and 5.8 for PMN, and intercept between 0.7 for PMN and 220.3 for MN. LoQ was comprised between 3.8×106 and 6.0×106 cells/L for WBC and PMN. Linearity was acceptable and carryover negligible.ConclusionsPF has a specific cellular composition. Overall, automated cell counting can only be suggested for total number of cells, whereas OM seems still the most reliable option for cell differentiation.

Algorithms for automated montage synthesis of images from laser-scanning confocal microscopes

1995 ◽  
Vol 53 ◽  
pp. 650-651
Author(s):  
D. E. Becker
Keyword(s):  
Image Analysis ◽  
High Resolution ◽  
Laser Scanning ◽  
Cell Counting ◽  
Wide Area ◽  
Data Set ◽  
Computational Synthesis ◽  
Automated Cell Counting ◽  
Partial View ◽  
The Individual

An efficient, robust, and widely-applicable technique is presented for computational synthesis of high-resolution, wide-area images of a specimen from a series of overlapping partial views. This technique can also be used to combine the results of various forms of image analysis, such as segmentation, automated cell counting, deblurring, and neuron tracing, to generate representations that are equivalent to processing the large wide-area image, rather than the individual partial views. This can be a first step towards quantitation of the higher-level tissue architecture. The computational approach overcomes mechanical limitations, such as hysterisis and backlash, of microscope stages. It also automates a procedure that is currently done manually. One application is the high-resolution visualization and/or quantitation of large batches of specimens that are much wider than the field of view of the microscope.The automated montage synthesis begins by computing a concise set of landmark points for each partial view. The type of landmarks used can vary greatly depending on the images of interest. In many cases, image analysis performed on each data set can provide useful landmarks. Even when no such “natural” landmarks are available, image processing can often provide useful landmarks.

scholarly journals The Role of Aerosol Pentamidine Prophylaxis

1993 ◽  
Vol 4 (2) ◽  
pp. 67-69
Author(s):  
E L C Ong
Keyword(s):  
Cell Count ◽  
Pneumocystis Carinii Pneumonia ◽  
Lymphocyte Count ◽  
Pneumocystis Carinii ◽  
Total Lymphocyte Count ◽  
First Episode ◽  
Cd4 Cell Count ◽  
Drug Of Choice ◽  
Cd4 Cell

Pneumocystis carinii pneumonia (PCP) is the most frequent opportunistic infection in patients with AIDS, occurring in 80% and recurring in 50% of patients within 12 months of the first episode. Prophylaxis for PCP is recommended if the CD4+ cell count is <200×106/l or 20% of the total lymphocyte count, or after an episode of PCP. The most effective prophylactic agent currently is trimethoprim-sulphamethoxazole and should be the drug of choice but alternatives such as aerosol pentamidine are being increasingly used for patients who cannot tolerate this combination or other oral preparations. If aerosol pentamidine is used and administered via a Respigard II Marquest nebulizer, the dosage should be higher than the currently recommended monthly dosage of 300 mg.

scholarly journals Local anesthetics impair the growth and self-renewal of glioblastoma stem cells by inhibiting ZDHHC15-mediated GP130 palmitoylation

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Xiaoqing Fan ◽  
Haoran Yang ◽  
Chenggang Zhao ◽  
Lizhu Hu ◽  
Delong Wang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Western Blot ◽  
Local Anesthetics ◽  
Molecular Mechanisms ◽  
Biological Activities ◽  
Xenograft Model ◽  
Cell Counting ◽  
Migration And Invasion ◽  
Self Renewal

Abstract Background A large number of preclinical studies have shown that local anesthetics have a direct inhibitory effect on tumor biological activities, including cell survival, proliferation, migration, and invasion. There are few studies on the role of local anesthetics in cancer stem cells. This study aimed to determine the possible role of local anesthetics in glioblastoma stem cell (GSC) self-renewal and the underlying molecular mechanisms. Methods The effects of local anesthetics in GSCs were investigated through in vitro and in vivo assays (i.e., Cell Counting Kit 8, spheroidal formation assay, double immunofluorescence, western blot, and xenograft model). The acyl-biotin exchange method (ABE) assay was identified proteins that are S-acylated by zinc finger Asp-His-His-Cys-type palmitoyltransferase 15 (ZDHHC15). Western blot, co-immunoprecipitation, and liquid chromatograph mass spectrometer-mass spectrometry assays were used to explore the mechanisms of ZDHHC15 in effects of local anesthetics in GSCs. Results In this study, we identified a novel mechanism through which local anesthetics can damage the malignant phenotype of glioma. We found that local anesthetics prilocaine, lidocaine, procaine, and ropivacaine can impair the survival and self-renewal of GSCs, especially the classic glioblastoma subtype. These findings suggest that local anesthetics may weaken ZDHHC15 transcripts and decrease GP130 palmitoylation levels and membrane localization, thus inhibiting the activation of IL-6/STAT3 signaling. Conclusions In conclusion, our work emphasizes that ZDHHC15 is a candidate therapeutic target, and local anesthetics are potential therapeutic options for glioblastoma.

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