scholarly journals Changes in Fusarium Link Species Composition From Lithuanian Wheat Grain in Years 2005-2007 to 2011-2013

2014 ◽  
Vol 32 (1) ◽  
pp. 45-50 ◽  
Author(s):  
Simonas Sakalauskas ◽  
Karina Stumbriene ◽  
Skaidre Suproniene ◽  
Povilas Svegzda

Abstract Changes in Fusarium species composition from naturally contaminated spring and winter wheat grain from the years 2005-2007 to 2011-2013 are presented. In general, Fusarium infection spring wheat grain was much more frequent than in winter wheat. In 2005-2007 F. avenaceum was dominant in both crops, with F. sporotrichioides, F. poae and F. culmorum isolated at lower levels, and F. graminearum, F. tricinctum, F. equiseti and F. oxysporum were occasionally found on both spring ant winter wheat. In 2011-2013, F. avenaceum lost its dominating status among Fusarium isolates in both crops, but the species composition of most frequently isolated fusaria in spring and winter wheat was quite different: F. graminearum, F. sporotrichioides, F. culmorum and F. avenaceum constituted the major part of fusaria isolated from spring wheat grain, while in winter wheat F. poae, F. sportotrichooides and the declining F. avenaceum were dominant.

2015 ◽  
Vol 31 (1) ◽  
pp. 123-131 ◽  
Author(s):  
V. Krnjaja ◽  
Z. Tomic ◽  
S. Stankovic ◽  
T. Petrovic ◽  
Z. Bijelic ◽  
...  

In this paper, the incidence of Fusarium-infected grain of winter wheat and the content of mycotoxin deoxynivalenol (DON) was studied in two Serbian cultivars Simonida and NS40S, both harvested in 2014. The level of Fusarium contamination of wheat grain was determined using phytopathological techniques based on the standard methodology while DON was detected by enzyme-linked immuno-sorbent assay (ELISA). The incidence of Fusarium-infected grain ranged from 12 to 19% for Simonida and NS40S, respectively. Fusarium graminearum, as well-known producer of DON mycotoxin, was identified among Fusarium species. In addition, Alternaria spp. was isolated in high percentage, with an average incidence of 53% (Simonida) to 63% (NS40S).The average content of DON ranged from 424 ?g kg-1 to 1101 ?g kg-1 for Simonida and NS40S cultivars, respectively. Statistically insignificant negative correlation (r = - 0.18) was determined between Fusariuminfected grain and DON in the cultivar Simonida and statistically insignificant positive correlation (r = 0.11) in the cultivar NS40S. The mean levels of DON in studied wheat samples of both tested cultivars were not higher than the maximum permitted limit (1250 ?g kg-1) although the level of Fusarium-infected grain of both cultivars was relatively high. These results indicate that both wheat cultivars are susceptible to Fusarium infection and DON mycotoxin production in agro-ecological conditions of Serbia, but the cultivar NS40S being more susceptible compared to cultivar Simonida. In view of all stated above, regular health check of grains and developing strategies for integrated monitoring of incidence of Fusarium head blight are necessary preventive measures in protection of winter wheat.


1980 ◽  
Vol 95 (3) ◽  
pp. 583-595 ◽  
Author(s):  
A. Penny ◽  
F. V. Widdowson

SUMMARYAn experiment at Rothamsted during 1958–67 measured effects on yield, on K uptake and on soil K of applying all combinations of 38, 75 and 113 kg N and 0, 31 and 62 kg K/ha per cut to grass leys, which were cut and removed. Soil K was depleted most where most N and least K were given. Annual applications of 0, 33 and 66 kg P/ha were also tested; soil P was not depleted. The grass was then ploughed.In 1968, residual effects were measured by spring wheat. In 1969 and in 1970 104 kg/ha of fresh K was applied on half of each plot; potatoes (1969) and spring wheat (1970) valued residual and fresh effects of K.In 1971 potatoes tested 0, 104 and 208 kg/ha of fresh K, cumulatively with the three amounts given to the grass and also extra K (104 kg/ha) on half-plots, cumulatively with that given in 1969 and 1970. In 1972 winter wheat, and in 1974 and 1975 spring barley, measured residues of all treatments previously applied (the site was fallowed in 1973).Finally, in 1976, potatoes tested 0, 156 and 312 kg/ha of fresh K on whole plots, cumulatively with the previous dressings of K, and also 156 kg/ha of extra K on half-plots, again cumulatively. All these test crops were given basal N.Yields and K contents of wheat at ear emergence and yields of wheat grain were largest after grass given 38 kg N and 62 kg K/ha per cut, because here soil K depletion was least. Wheat grain yields benefited consistently from fresh K. K content of the wheat at ear emergence was a good indicator of the need for K, but K content of grain was not, because it was unaltered by K fertilizer. Barley was a poor test crop for K, because yields of grain were little affected by previous treatments.Percentage K in potato leaves (in July in 1969 and 1971, in August in 1976) and yield of tubers were well correlated. Largest yields in 1969, 1971 and 1976 came where the leaves contained 3·43, 3·76 and 2·82% K, respectively, i.e. from soil containing most exchangeable K, plus most fresh K. There was no indication that maximum yields had been obtained, so the largest amounts (kg/ha) of fresh K tested (104 in 1969, 312 in 1971 and 468 in 1976) were insufficient to counteract depletion of soil K by the grass. Because the grass did not deplete soil P, the test crops benefited only little from either residual or fresh P.


Author(s):  
Vesna Krnjaja ◽  
Jelena Levic ◽  
Zorica Nesic ◽  
Slavica Stankovic

Effects of fertilisers on the grain yield (t ha-1) and the development of mycobiota, especially of the Fusarium species, in winter wheat kernels were observed. Fertilisers were applied in the following variants: T1 - 30m3 of slurry (pre-sowing treatment) and 25 m3 of slurry (top-dressing), T2 - 22.500 kg of manure (pre-sowing treatment) and 220 kg of urea (top-dressing), T3 - 300 kg of urea (top-dressing) and T4 - the control without application of fertilisers. The average wheat grain yield was the highest in the treatment T2 (6.9 t ha-1), then in T3 (6.3 t ha-1) and T1 (6.2 t ha-1), while the lowest wheat grain yield (4.3 t ha-1) was registered in the control (T4). Fungi of genera Alternaria (88.8-96.3%) and Fusarium (3.7-11.1%) were mainly isolated from all wheat kernel samples collected after harvest (T1-T4). Species of the genus Dreschlera were isolated in treatments T1 (0.3%) and T2 (0.2%). The species Stemphylium botryosum was isolated only in the treatment T1 (0.2%). F. graminearum (3.5-10.8%) was isolated from wheat kernels in all observed treatments, while F. sporotrichioides was isolated in treatments T1 (0.6%), T2 (0.5%) and T3 (0.3%). F. poae (0.3%) and F. subglutinans (0.2%) were isolated in the treatment T2, while F. graminearum was predominantly present in treatments T3 (10.8%), T1 (8.7%) and T2 (7.8%), and in the control T4 (3.5%). The obtained results point out that the application of urea affected the decrease in the intensity of Alternaria species infestation, but it also affected the increase of frequency of Fusarium species, especially F. graminearum, in comparison with the control and other treatments. Negative correlation was registered between the grain yield and the intensity of infestation of Fusarium species (collectively) and F. graminearum (individually).


2019 ◽  
pp. 74-76
Author(s):  
G. N. Buchneva ◽  
I. V. Gusev ◽  
O. I. Korabelskaya ◽  
N. N. Dubrovskaya ◽  
V. V. Chekmarev

In the climatic conditions of the Central Black-earth region, the infection of wheat grain with Fusarium fungi is of latent nature and can only be detected by mycological analysis. For this reason, the varietal composition of the pathogenic complex of fungi Fusarium on winter and spring wheat has not been thoroughly studied yet. Working with the problem in the conditions of the Tambov region, it was found that the grain of these varieties was infected with various species of Fusarium. The most infected spring wheat varieties were “Nik” (69%), “Biora” (45%) and “Prokhorovka” (30%). The varieties “Saratovskaya 29” (4%) and “Tulaikovskaya 100” (5%) were less infected than the other. Nine species of fungus Fusarium spp. found on spring wheat seeds were F. acuminatum Ellis, an Everhart (1895), a Wollenweber (1917), F. avenaceum (Corda ex Fries) Saccardo (1886), F. culmorum (WG Smith) Saccardo  (1895), F. equiseti (Corda) Saccardo (1886), F. poae (Peck) Wollenweber in Lewis (1913), F. sambucinum Fuckel (1869), F. semitectum Berkeley and Ravenel in Berkeley (1875), F. sporotrichioides Sherbakoff (1915) and F. tricinctum (Corda) Saccardo (1886). The leading position was occupied by the species Fusarium sporotrichioides and F. avenaceum. The frequency of their occurrence on spring wheat seeds was 56.6 and 20.9%, respectively. The contamination of winter wheat with a Fusarium fungus was lower than the infection of spring wheat (1–8%). Four species of Fusarium spp. were identified on the seeds of winter wheat, they are F. avenaceum (Corda ex Fries) Saccardo (1886), F. graminearum Schwabe (1838), F. poae (Peck) Wollenweber in Lewis (1913) and F. sporotrichioides Sherbakoff (1915). The dominant position belonged to the species Fusarium poae and F. sporotrichioides (41.7 and 37.5%). It has been shown that the level of infection of wheat seeds by Fusarium fungi depends on the variety resistance to the infection.


2011 ◽  
Vol 64 (1) ◽  
pp. 3-13 ◽  
Author(s):  
J. Czaban ◽  
B. Wróblewska ◽  
A. Sułek ◽  
G. Podolska

Colonization of wheat grain by Fusaria in two crop management systems varying in intensity of production technology The purpose of this study was to investigate the effects of two cropping systems (intensive and integrated) on infection level of winter and spring wheat kernels by Fusarium species. Field experiments were conducted with winter wheat ‘Tonacja’ and spring wheat ‘Bombona’ during two consecutive growing seasons (2007/2008 and 2008/2009 - winter wheat, and 2008 and 2009 - spring wheat). A rainfall level in 2009 from the last decade of May to the first decade of July was 2.5-times higher than that in 2008. After the harvest, kernels were surface disinfected with 1.5% NaOCl solution for 2 min and then analysed for the infection level by different species of Fusarium. Fusaria were isolated on CZID medium and identified on the basis of macro - and micro-morphology on three media (PDA, SNA and a medium containing tannin). Our results demonstrate that the wheat grain infection by Fusarium depended mainly on a rainfall level. The intensive cropping system was more conducive to the grain infection by fusaria in comparison to the integrated one. The most frequent species were F. avenaceum in 2008, and F. graminearum, F. avenaceum and F. poae in 2009.


2020 ◽  
Vol 8 (4) ◽  
pp. 617
Author(s):  
Tim Birr ◽  
Mario Hasler ◽  
Joseph-Alexander Verreet ◽  
Holger Klink

Fusarium head blight (FHB) is one of the most important diseases of wheat, causing yield losses and mycotoxin contamination of harvested grain. A complex of different toxigenic Fusarium species is responsible for FHB and the composition and predominance of species within the FHB complex are determined by meteorological and agronomic factors. In this study, grain of three different susceptible winter wheat cultivars from seven locations in northern Germany were analysed within a five-year survey from 2013 to 2017 by quantifying DNA amounts of different species within the Fusarium community as well as deoxynivalenol (DON) and zearalenone (ZEA) concentrations. Several Fusarium species co-occur in wheat grain samples in all years and cultivars. F. graminearum was the most prevalent species, followed by F. culmorum, F. avenaceum and F. poae, while F. tricinctum and F. langsethiae played only a subordinate role in the FHB complex in terms of DNA amounts. In all cultivars, a comparable year-specific quantitative occurrence of the six detected species and mycotoxin concentrations were found, but with decreased DNA amounts and mycotoxin concentrations in the more tolerant cultivars, especially in years with higher disease pressure. In all years, similar percentages of DNA amounts of the six species to the total Fusarium DNA amount of all detected species were found between the three cultivars for each species, with F. graminearum being the most dominant species. Differences in DNA amounts and DON and ZEA concentrations between growing seasons depended mainly on moisture factors during flowering of wheat, while high precipitation and relative humidity were the crucial meteorological factors for infection of wheat grain by Fusarium. Highly positive correlations were found between the meteorological variables precipitation and relative humidity and DNA amounts of F. graminearum, DON and ZEA concentrations during flowering, whereas the corresponding correlations were much weaker several days before (heading) and after flowering (early and late milk stage).


2005 ◽  
Vol 95 (10) ◽  
pp. 1225-1236 ◽  
Author(s):  
P. A. Paul ◽  
P. E. Lipps ◽  
L. V. Madden

The association between Fusarium head blight (FHB) intensity and deoxynivalenol (DON) accumulation in harvested grain is not fully understood. A quantitative review of research findings was performed to determine if there was a consistent and significant relationship between measures of Fusarium head blight intensity and DON in harvested wheat grain. Results from published and unpublished studies reporting correlations between DON and Fusarium head blight “index” (IND; field or plot-level disease severity), incidence (INC), diseased-head severity (DHS), and Fusarium-damaged kernels (FDK) were analyzed using meta-analysis to determine the overall magnitude, significance, and precision of these associations. A total of 163 studies was analyzed, with estimated correlation coefficients (r) between -0.58 and 0.99. More than 65% of all r values were >0.50, whereas less that 7% were <0. The overall mean correlation coefficients for all relationships between DON and disease intensity were significantly different from zero (P < 0.001). Based on the analysis of Fisher-transformed r values ( zr values), FDK had the strongest relationship with DON, with a mean r of 0.73, followed by IND (r = 0.62), DHS (r = 0.53), and INC (r = 0.52). The mean difference between pairs of transformed zr values (zd ) was significantly different from zero for all pairwise comparisons, except the comparison between INC and DHS. Transformed correlations were significantly affected by wheat type (spring versus winter wheat), study type (fungicide versus genotype trials), and study location (U.S. spring- and winter-wheat-growing regions, and other wheat-growing regions). The strongest correlations were observed in studies with spring wheat cultivars, in fungicide trials, and in studies conducted in U.S. spring-wheat-growing regions. There were minor effects of magnitude of disease intensity (and indirectly, environment) on the transformed correlations.


Author(s):  
Maryse Bourgault ◽  
Samuel A. Wyffels ◽  
Julia M. Dafoe ◽  
Peggy F. Lamb ◽  
Darrin L. Boss

Abstract The introduction of cover crops as fallow replacement in the traditional cereal-based cropping system of the Northern Great Plains has the potential to decrease soil erosion, increase water infiltration, reduce weed pressure and improve soil health. However, there are concerns this might come at the cost of reduced production in the subsequent wheat crop due to soil water use by the cover crops. To determine this risk, a phased 2-year rotation of 15 different cover crop mixtures and winter wheat/spring wheat was established at the Northern Agricultural Research Center near Havre, MT from 2012 to 2020, or four rotation cycles. Controls included fallow–wheat and barley–wheat sequences. Cover crops and barley were terminated early July by haying, grazing or herbicide application. Yields were significantly decreased in wheat following cover crops in 3 out of 8 years, up to maximum of 1.4 t ha−1 (or 60%) for winter wheat following cool-season cover crop mixtures. However, cover crops also unexpectedly increased following wheat yields in 2018, possibly due in part to residual fertilizer. Within cool-, mid- and warm-season cover crop groups, individual mixtures did not show significant differences impact on following grain yields. Similarly, cover crop termination methods had no impact on spring or winter wheat grain yields in any of the 8 years considered. Wheat grain protein concentration was not affected by cover crop mixtures or termination treatments but was decreased in winter wheat following barley. Differences in soil water content across cover crop groups were only evident at the beginning of the third cycle in one field, but important reductions were observed below 15 cm in the last rotation cycle. In-season rainfall explained 43 and 13% of the variability in winter and spring wheat yields, respectively, compared to 2 and 1% for the previous year cover crop biomass. Further economic analyses are required to determine if the integration of livestock is necessary to mitigate the risks associated with the introduction of cover crops in replacement of fallow in the Northern Great Plains.


2018 ◽  
Vol 64 (No. 12) ◽  
pp. 592-596 ◽  
Author(s):  
Agnieszka Pszczółkowska ◽  
Adam Okorski ◽  
Jacek Olszewski ◽  
Gabriel Fordoński ◽  
Sławomir Krzebietke ◽  
...  

The after-effects of pre-preceding crops (second year), i.e. legumes and spring wheat, and nitrogen fertilization rate (0, 60, 120 and 180 kg N/ha) on the yield and chemical composition of winter wheat grain were analysed in a field experiment conducted in 2013–2015. Winter wheat was characterized by higher yield when sown after blue lupine (increase of 0.23 t/ha) and faba beans with a determinate growth habit (increase of 0.37 t/ha) than after spring wheat. Grain yield increased significantly with a rise in nitrogen fertilization rate (by 2.03, 3.47 and 4.02 t/ha, respectively). The species of pre-preceding crops had no significant effect on the phosphorus, potassium, magnesium and calcium content of winter wheat grain. Winter wheat grown after faba beans with an indeterminate growth habit was most abundant in nitrogen. The applied nitrogen fertilizer rates did not modify the concentrations of phosphorus, magnesium and calcium in winter wheat grain. The nitrogen content of grain increased significantly with a rise in nitrogen fertilization rates. A significant increase in manganese and zinc levels was observed when spring wheat was the pre-preceding crop and the iron content of grain increased significantly when winter wheat was grown after peas and blue lupine.


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