scholarly journals Changes in microbiological properties of soil during fungicide degradation

2018 ◽  
Vol 69 (3) ◽  
pp. 169-176
Author(s):  
Małgorzata Baćmaga ◽  
Jadwiga Wyszkowska ◽  
Jan Kucharski ◽  
Piotr Kaczyński
Keyword(s):  
Bacterial Diversity ◽  
Structural Diversity ◽  
Biochemical Properties ◽  
Fungicide Residues ◽  
The Family ◽  
Liquid Chromatography Tandem Mass ◽  
Biochemical Analyses ◽  
Almost All ◽  
Next Generation Sequencing Ngs ◽  
Properties Of Soil

Abstract Laboratory tests were performed on sandy loamy soil to establish the relations between bacterial diversity, soil enzyme activity and degradation of Amistar 250 SC, Falcon 460 EC and Gwarant 500 SC fungicides. Apart from carrying out microbiological and biochemical analyses, the residues of active substances from the tested fungicides were determined. Structural diversity of was determined based on the next-generation sequencing (NGS) method, and fungicide residues the liquid chromatography tandem-mass spectrometry (LC-MS/MS). It was found that changes in bacterial diversity occurred in the soil subject to fungicide treatment, particularly at the family and genus level. Proteobacteria, Firmicutes and Actinobacteria were prevailing in all soil samples. Bacillus occurred both in the control soil and in the soil treated with fungicides, while Pseudonocardia occurred only in the fungicide-treated soil. Of all the fungicides tested, the biggest changes in bacterial diversity were caused by Gwarant 500 SC. The preparations tested not only affected the composition of soil microbiota, but also contributed to changes in the biochemical properties of soil by inhibiting the activity of almost all tested enzymes, with the exception of alkaline phosphatase and β-glucosidase. Chlorothalinil was the fastest degraded in the soil and spiroxamine at the slowest.

Analisis Perbandingan Manajemen Keuangan Ojek Online Dan Ojek Reguler Studi Kasus Kecamatan Duren Sawit Jakarta Timur

Liquidity ◽  
2018 ◽  
Vol 7 (1) ◽  
pp. 41-52
Author(s):  
M. Koesmawan ◽  
Darwin Erhandy ◽  
Dede Dahlan
Keyword(s):  
Financial Management ◽  
Average Income ◽  
Savings And Loans ◽  
Financial Difficulties ◽  
The Family ◽  
Family Finances ◽  
The Subject ◽  
Almost All

In order to meet the needs of living which consists of primary as well as secondary needs, human can work in either a formal or an informal job. One of the informal jobs that is became the subject of this research was to become an ojek driver. Ojek is a ranting motorcycle.  Revenue of ojek drivers, accordingly, should be well managed following the concept of financial management. This research was conducted for the driver of the online motorcycle drivers as well as the regular motorcycle drivers they are called “The Ojek”. Ojek’s location is in Kecamatan (subdistrict) Duren Sawit, East Jakarta with 70 drivers of ojeks. The online ojeks earn an average of Rp 100,000 per day, can save Rp 11,000 to 21,000 per day, while, the regular ojek has an average income per day slightly lower amounted to Rp 78,500, this kind of ojeks generally have other businesses and always record the outflow of theirs money. Both the online and regular ojeks feel a tight competition in getting passengers, but their income can help the family finances and both ojeks want a cooperative especially savings and loans, especially to overcome the urgent financial difficulties. Almost all rivers, do not dare to borrow money. They are afraid of can not refund the money as scheduled.

scholarly journals Optimization of a WGA-Free Molecular Tagging-Based NGS Protocol for CTCs Mutational Profiling

2020 ◽  
Vol 21 (12) ◽  
pp. 4364
Author(s):  
Giuseppa De Luca ◽  
Barbara Cardinali ◽  
Lucia Del Mastro ◽  
Sonia Lastraioli ◽  
Franca Carli ◽  
...  
Keyword(s):  
Substantial Reduction ◽  
Breast Cancer Patients ◽  
Dna Templates ◽  
Molecular Tagging ◽  
Mutational Profiling ◽  
Optimized Protocol ◽  
Set Up ◽  
Detection Of Mutations ◽  
Almost All ◽  
Next Generation Sequencing Ngs

Molecular characterization of Circulating Tumor Cells (CTCs) is still challenging, despite attempts to minimize the drawbacks of Whole Genome Amplification (WGA). In this paper, we propose a Next-Generation Sequencing (NGS) optimized protocol based on molecular tagging technology, in order to detect CTCs mutations while skipping the WGA step. MDA-MB-231 and MCF-7 cell lines, as well as leukocytes, were sorted into pools (2–5 cells) using a DEPArray™ system and were employed to set up the overall NGS procedure. A substantial reduction of reagent volume for the preparation of libraries was performed, in order to fit the limited DNA templates directly derived from cell lysates. Known variants in TP53, KRAS, and PIK3CA genes were detected in almost all the cell line pools (35/37 pools, 94.6%). No additional alterations, other than those which were expected, were found in all tested pools and no mutations were detected in leukocytes. The translational value of the optimized NGS workflow is confirmed by sequencing CTCs pools isolated from eight breast cancer patients and through the successful detection of variants. In conclusion, this study shows that the proposed NGS molecular tagging approach is technically feasible and, compared to traditional NGS approaches, has the advantage of filtering out the artifacts generated during library amplification, allowing for the reliable detection of mutations and, thus, making it highly promising for clinical use.

Two new species of the genus Syngastes (Copepoda, Harpacticoida, Tegastidae) from South Korea

Crustaceana ◽  
2016 ◽  
Vol 89 (4) ◽  
pp. 431-458 ◽  
Author(s):  
Jong Guk Kim ◽  
Jong Guk Kim ◽  
Tae Won Jung ◽  
Jong Guk Kim ◽  
Tae Won Jung ◽  
...  
Keyword(s):  
New Species ◽  
South Korea ◽  
First Record ◽  
Exopodal Segment ◽  
Key To Species ◽  
Korean Waters ◽  
The Family ◽  
Two New Species ◽  
Almost All

Herein two new species of the genusSyngastesMonard, 1924 are described from South Korea, with detailed descriptions and illustrations. Both new copepods,Syngastesmulticavussp. nov. andS. pseudofoveatussp. nov., have two inner setae on the first exopodal segment of P2 and P3.Syngastesmulticavussp. nov. most closely resemblesS. gibbosusBartsch, 1999 reported from Australia, as they both have a five-segmented antennule in the female. However,Syngastesmulticavussp. nov. has a rounded body outline instead of the gibbose outline observed inS. gibbosus.Syngastespseudofoveatussp. nov. resemblesS. foveatusBartsch, 1994 in almost all aspects. However, they differ clearly in the number of setae on the first exopodal segment of P2 and P3. We also provide a key to species of the genusSyngastesworldwide. The present study is the first record of the family Tegastidae in Korean waters.

Resolving the Lourinia armata (Claus, 1866) complex with remarks on the monophyletic status of Louriniidae, Monard 1927 (Copepoda: Harpacticoida)

Zootaxa ◽  
2021 ◽  
Vol 5051 (1) ◽  
pp. 346-386
Author(s):  
SÜPHAN KARAYTUĞ ◽  
SERDAR SAK ◽  
ALP ALPER ◽  
SERDAR SÖNMEZ
Keyword(s):  
Comparative Evaluation ◽  
Species Complex ◽  
Detailed Examination ◽  
The Other ◽  
Phylogenetic Position ◽  
Nicobar Island ◽  
Other Hand ◽  
The Family ◽  
Close Relationship ◽  
Almost All

An attempt was made to test if Lourinia armata (Claus, 1866)—as it is currently diagnosed—represents a species complex. Detailed examination and comparisons of several specimens collected from different localities suggest that L. armata indeed represents a complex of four closely related morphospecies that can be differentiated from one another by only detailed observations. One of the four species is identified as Lourinia aff. armata and the other three species are described as new to science and named as Lourinia wellsi sp. nov., L. gocmeni sp. nov., and L. aldabraensis sp. nov. Detailed review of previous species records indicates that the genus Lourinia Wilson, 1924 is distributed worldwide. Ceyloniella nicobarica Sewell, 1940, originally described from Nicobar Island and previously considered a junior subjective synonym of L. armata is reinstated as Lourinia nicobarica (Sewell, 1940) comb. nov. on the basis of the unique paddle-shaped caudal ramus seta V. It is postulated that almost all of these records are unreliable in terms of representing true Lourinia aff. armata described herein. On the other hand, the comparative evaluation of the illustrations and descriptions in the published literature indicates the presence of several new species waiting to be discovered in the genus Lourinia.                 It has been determined that, according to updated modern keys, the recent inclusion of the monotypic genus Archeolourinia Corgosinho & Schizas, 2013 in the Louriniidae is not justified since Archeolourinia shermani Corgosinho & Schizas, 2013 does not belong to this family but should be assigned to the Canthocamptidae. On the other hand, it has been argued that the exact phylogenetic position of the Louriniidae still remains problematic since none of the diagnostic characters supports the monophyly of the family within the Oligoarthra. It has also been argued that the close relationship between Louriniidae and Canthocamptidae is supported since both families share the homologous sexual dimorphism (apophysis) on P3 endopod. The most important characteristic that can possibly be used to define Louriniidae is the reduction of maxilliped.  

Subcellular localization and biochemical properties of the enzymes of carbamoyl phosphate and urea synthesis in the batrachoidid fishes Opsanus beta, Opsanus tau and Porichthys notatus

1995 ◽  
Vol 198 (3) ◽  
pp. 755-766 ◽  
Author(s):  
P Walsh
Keyword(s):  
Glutamine Synthetase ◽  
Subcellular Localization ◽  
Biochemical Properties ◽  
Nitrogen Excretion ◽  
Urea Synthesis ◽  
Carbamoyl Phosphate ◽  
Opsanus Beta ◽  
Opsanus Tau ◽  
Porichthys Notatus ◽  
The Family

The subcellular localization and biochemical properties of the enzymes of carbamoyl phosphate and urea synthesis were examined in three representatives of fishes of the family Batrachoididae, the gulf toadfish (Opsanus beta), the oyster toadfish (Opsanus tau) and the plainfin midshipman (Porichthys notatus). The primary objective of the study was to compare the biochemical characteristics of these fishes, which represent a range between ammoniotelism and ureotelism (O. beta being facultatively ureotelic), with previous patterns observed for an ammoniotelic teleost (Micropterus salmoides, the largemouth bass) and an obligate ureogenic elasmobranch (Squalus acanthias, the dogfish shark). The present study documents the expression of mitochondrial carbamoyl phosphate synthetase (CPSase) III and cytosolic CPSase II (and its associated enzymes of pyrimidine synthesis, dihydro-orotase and aspartate carbamoyltransferase) in the livers of all three batrachoidid species. Both mitochondrial and cytosolic activities of arginase were present in the livers of all three species, as were cytosolic glutamine synthetase and argininosuccinate synthetase and lyase. However, O. beta also showed mitochondrial glutamine synthetase activity and higher total hepatic levels of glutamine synthetase than either O. tau or P. notatus. Taken together, these observations confirm that the arrangement of these enzymes in the batrachoidid fishes has greater similarity to that of M. salmoides than to that of S. acanthias. However, differences within the family appear to coincide with the different nitrogen excretion strategies. O. tau and P. notatus are primarily ammoniotelic and most closely resemble the ammoniotelic M. salmoides, whereas ureotelism in O. beta is correlated with the presence of a mitochondrial glutamine synthetase and the ability to induce higher total glutamine synthetase activities than O. tau or P. notatus. Additionally, isolated mitochondria from O. beta were able to generate citrulline from glutamine, whereas those from O. tau were not. Also in contrast to S. acanthias, glutamine synthetase activities in the mitochondria of O. beta are consistently lower than those of CPSase III. This and other kinetic observations lend support to the hypothesis that glutamine synthetase may be an important regulatory control point in determining rates of ureogenesis in O. beta.

THE PATIENT BOY—A POPULAR LESSON, READ TO CHILDREN IN THE 1820'S

PEDIATRICS ◽  
1969 ◽  
Vol 43 (4) ◽  
pp. 526-526
Author(s):  
T. E. C.
Keyword(s):  
The Family ◽  
Almost All

The story below depicts a favorite subject of those who wrote for children in the early days of our country. The inebriated father leading his family inexorably into destitution by his intemperance is counterbalanced by a son who holds the family together. During the last century many children cried over this and similar stories. The Patient Boy There was a journeyman bricklayer in this town, a good workman, but a very drunken idle fellow; he spent at the dram shop almost all he earned, and left his wife and children to take care of themselves; to get food and clothes as they could. They might all have starved, but for the eldest son, whom his father had brought up to help him at his work; and who was so industrious and attentive, that being now at the age of thirteen or fourteen, he was able to earn pretty good wages, every penny of which that he could keep out of his father's hands, he brought to his mother. When the brute of a father came home drunk, cursing and swearing, and in such an ill humour that his mother, and the rest of the children, durst not come near him for fear of a beating, this good lad (Tom was his name) kept close to him, to pacify him, and get him quietly to bed. His mother looked upon Tom as the support of the family, and loved him dearly. It happened one day, Tom in climbing up a high ladder, with a load of mortar on his head, missed his hold, and fell down to the bottom, on a heap of bricks and rubbish.

Transmission dynamics and mechanisms of endemicity of scrapie in the UK sheep population

2008 ◽  
Vol 137 (6) ◽  
pp. 762-774 ◽  
Author(s):  
J. E. TRUSCOTT ◽  
N. M. FERGUSON
Keyword(s):  
Structural Diversity ◽  
Epidemiological Data ◽  
Transmissible Spongiform Encephalopathies ◽  
Spongiform Encephalopathies ◽  
Sheep Population ◽  
Mean Values ◽  
The United Kingdom ◽  
The Family ◽  
The Uk

SUMMARYScrapie is a fatal neurological disease of sheep which is endemic in the United Kingdom. It is one of the family of transmissible spongiform encephalopathies (TSEs) that includes BSE. In this paper, we developed a micro-simulation model for scrapie in the UK sheep population, incorporating the genetic and structural diversity of the population and infectious contact between flocks through trading. The simulation was fitted to epidemiological data from a range of sources. We found a detection/reporting probability of 16% (95% CI 12–17) for animals dying of scrapie. Prevalence of infected animals in the population was about 0·15%. Infected individuals were found in 9% of flocks overall, rising to 60% in Shetland and 75% in Swaledale flocks. Mean values of R0 for flocks varied with breed from 2·43 (Shetland) to 0·21 (Suffolk). We also examined the possible long-term persistence of scrapie in the UK flock in the absence of any intervention.

Vincent Noce, La Collection Egoiste (The Selfish Collector)

2006 ◽  
Vol 13 (1) ◽  
pp. 115-119
Author(s):  
Lyndel V. Prott
Keyword(s):  
New York ◽  
New York Times ◽  
Illicit Trade ◽  
The Family ◽  
Almost All

Vincent Noce, La Collection Egoiste (The Selfish Collector) pp. 328. J. C. Lattès, Paris, 2005. ISBN 2-7096-241-9.Few people who follow cases relating to the illicit trade can have missed the celebrated case of Stéfane Breitwieser, the Alsatian misfit who stole, over a period of 8 or so years, hundreds of objects from museums and churches to squirrel away in his attic rooms, or that of his mother Mireille Stengel, who destroyed almost all of it by disposal in the family garbage bin or by throwing it into a canal. This book, however, shows just how much a dedicated investigative journalist can add to the record, details that are not only useful in trying to understand the mentality of Breitwieser (by no means an isolated case as this account shows) and even more useful in showing the loopholes in the investigations, the lack of coordination between countries, and the sheer ineptitude of many institutions in securing their collections. Noce, editor of the cultural section of the French newspaper Libération, has joined the select company of Karl Meyer (articles in the New York Times) and Peter Watson who have added greatly to our knowledge of how the illicit trade works. French journalists, too, are greatly helping expose the unsavory details of these activities (see Noce's previous book Descente aux Enchères and that of Emmanuel de Roux and Roland-Pierre Paringaud, Razzia sur L'art).

scholarly journals Quinolizidine-Based Variations and Antifungal Activity of Eight Lupinus Species Grown under Greenhouse Conditions

Molecules ◽  
2022 ◽  
Vol 27 (1) ◽  
pp. 305
Author(s):  
Willy Cely-Veloza ◽  
Diego Quiroga ◽  
Ericsson Coy-Barrera
Keyword(s):  
Antifungal Activity ◽  
Biological Activities ◽  
Structural Diversity ◽  
Economic Losses ◽  
Quinolizidine Alkaloids ◽  
Mycelium Growth ◽  
Reference Information ◽  
Naturally Occurring ◽  
The Family ◽  
External Standard

Fusarium oxysporum is an aggressive phytopathogen that affects various plant species, resulting in extensive local and global economic losses. Therefore, the search for competent alternatives is a constant pursuit. Quinolizidine alkaloids (QA) are naturally occurring compounds with diverse biological activities. The structural diversity of quinolizidines is mainly contributed by species of the family Fabaceae, particularly the genus Lupinus. This quinolizidine-based chemo diversity can be explored to find antifungals and even mixtures to address concomitant effects on F. oxysporum. Thus, the antifungal activity of quinolizidine-rich extracts (QREs) from the leaves of eight greenhouse-propagated Lupinus species was evaluated to outline promising QA mixtures against F. oxysporum. Thirteen main compounds were identified and quantified using an external standard. Quantitative analysis revealed different contents per quinolizidine depending on the Lupinus plant, ranging from 0.003 to 32.8 mg/g fresh leaves. Bioautography showed that all extracts were active at the maximum concentration (5 µg/µL). They also exhibited >50% mycelium growth inhibition. All QREs were fungistatic except for the fungicidal QRE of L. polyphyllus Lindl. Angustifoline, matrine, 13α-hydroxylupanine, and 17-oxolupanine were ranked to act jointly against the phytopathogen. Our findings constitute reference information to better understand the antifungal activity of naturally afforded QA mixtures from these globally important plants.

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