scholarly journals Potential of Streptomyces sp. in preventing the in vitro growth of Colletotrichum acutatum, the causative agent of infection in Capsicum annum L.

2021 ◽  
Vol 25 (2) ◽  
pp. 197
Author(s):  
Rochmalia Juniarti Putri ◽  
Retno Kawuri ◽  
Anak Agung Ketut Darmadi ◽  
Inna Narayani
Keyword(s):  
Culture Method ◽  
Colletotrichum Acutatum ◽  
Diffusion Method ◽  
Dilution Method ◽  
Dual Culture ◽  
Vegetable Crops ◽  
Streptomyces Sp ◽  
Chilli Plant ◽  
Capsicum Annum

Red chilli plant (Capsicum annum L.) is one of the most popular vegetable crops in Indonesian society. One of them the pathogens attacks is Colletotrichum acutatum, a fungus causing anthracnose on red chilli. This study aims to determine the existence of Streptomyces sp. bacteria in the rhizosphere of the red chilli plant; the ability of Streptomyces sp. in inhibiting C. acutatum; Minimum Inhibitory Concentration (MIC) of Streptomyces isolates extracts in inhibiting C. acutatum; The Streptomyces isolation was carried out by dilution method using selective meida, namely Yeast Malt Agar. The Dual Culture method was used as an inhibition test between Streptomyces sp. and C. acutatum in vitro. A well diffusion method was used to test the effectiveness of the Streptomyces sp. and MIC filtrate concentration in inhibiting C. acutatum. The data obtained in this study were analyzed with Analysis of Varian (ANOVA) then continued with Duncan Multiple Range Test with 5% significance. Five Streptomyces isolates were found, namely Streptomyces sp.1, Streptomyces sp.2, Streptomyces sp.3, Streptomyces sp.4, and Streptomyces sp.5 in the rhizosphere of healthy C. annum L. plants in Daup Village, Kintamani District, Bangli Regency. Streptomyces sp. isolates. can significantly inhibit the growth of the fungus C. acuatum with inhibitory power ranging from 50.30% to 83.76%, Streptomyces sp.5 isolate was able to provide the highest percentage of inhibition in C. acutatum of 83.76 ± 2.91% with MIC 7% (v/v) with a diameter of 6.40 mm.

scholarly journals EKSPLORASI DAN IDENTIFIKASI CENDAWAN ANTAGONIS TERHADAP JAMUR AKAR PUTIH (Rigidoporus lignosus) PADA TANAMAN PALA (Myristica fragrans Houtt)

2020 ◽  
Vol 4 (2) ◽  
Author(s):  
Nana Ariska ◽  
Lola Adres Yanti ◽  
Chairudin Chairudin
Keyword(s):  
Economic Value ◽  
Culture Method ◽  
Dilution Method ◽  
Dual Culture ◽  
Trichoderma Spp ◽  
Pests And Diseases ◽  
Myristica Fragrans ◽  
Rigidoporus Lignosus ◽  
Myristica Fragrans Houtt

Nutmeg (Myristica fragrans Houtt) is a multipurpose plantation commodity crop that almost all parts of the plant have strategic economic value to be cultivated. In Indonesia there are the largest nutmeg commodity centers, including the Maluku islands, North Sulawesi and Aceh. The biggest problem with nutmeg plants is the attack of pests and diseases. One of the most severe diseases in nutmeg is white root mushroom (Rigidoporus lignosus). This study aims to 1) determine the fungus of white root fungus antagonists (Rigodoporus lignosus) from the Aceh nutmeg rhizosphere (Myristica fragrans Houtt), 2) identify the content of antibiotic fungus JAP antagonist compounds in Aceh nutmeg plants. The study was conducted by taking soil samples from nutmeg plants at three nutmeg plantations in South Aceh. The antagonist fungus isolation activity uses a dilution method. Isolation of JAP from infected roots using direct planting method. Furthermore, it is identified macroscopically and microscopically. The antagonist test was carried out using the dual culture method with RAL. From the rhizosphere in the nutmeg area, 5 candidate antagonist isolates were obtained, having high in vitro antagonism ability to the pathogen R. lignosus. Identification results showed that isolates 1, 2 and 3 were Trichoderma spp, and isolates 4 and 5 were Gliocladium virens. Keywords: In vitro, the content of antibiotic compounds, Aceh nutmeg, Rigidoporus lignosus, antagonistic test

scholarly journals Evaluation of possible biological control of Fusarium sp. using plant extracts and antagonistic species of microbes in vitro

F1000Research ◽  
2020 ◽  
Vol 9 ◽  
pp. 1394
Author(s):  
Mohammed Faruk Hasan ◽  
Mohammed Asadul Islam ◽  
Biswanath Sikdar
Keyword(s):  
Plant Extracts ◽  
Control Measure ◽  
Trichoderma Viride ◽  
Culture Method ◽  
Dual Culture ◽  
Vegetable Crops ◽  
Fruit And Vegetable ◽  
Medicinal Plant Extracts ◽  
Plant Leaf Extract

Background: Fusarium species is one of the most devastating fungi responsible for fruit and vegetable crops rot worldwide. The present study was designed to find an ecofriendly control measure for pathogenic Fusarium species, using suitable bioagents. Methods: Medicinal plant extracts were evaluated or their antifungal activities against Fusarium species using the poisoned food method. Antagonistic potency of some nonpathogenic microbes was also assessed on Fusarium species using the dual culture method. Results: Highest inhibition of growth of Fusarium sp. was observed with 68.1% (0.389 mg per 90 mm Petri plate) of mycelia on Coccinia grandis plant leaf extract, in comparison to the control grown with 100.0% (1.22 mg/dish). The highest inhibition of radial growth was observed using Trichoderma viride on Fusarium sp. (46.01% inhibition). Conclusions: The findings of present study would be benevolent for antifungal drug development to control Fusarium sp. causing fruit and vegetable rot.

scholarly journals Utilization of In Situ Antagonistic Fungus as A Biocontrol Agent of Lasiodiplodia theobromae Causes of Dieback Disease on The Nutmeg Tree in Aceh Selatan

1970 ◽  
Vol 5 (3) ◽  
pp. 447-454
Author(s):  
Susanna ◽  
Meity S. Sinaga ◽  
Suryo Wiyono ◽  
Hermanu Triwidodo
Keyword(s):  
Soil Fungus ◽  
Culture Method ◽  
Dilution Method ◽  
Dual Culture ◽  
Lasiodiplodia Theobromae ◽  
Antagonistic Fungi ◽  
Dieback Disease ◽  
Inhibitory Power

Dieback disease caused by Lasiodiplodia theobromae is an important disease on the nutmeg tree in Aceh Selatan. The disease has been widespread in the district. One alternative to control the disease is the use of antagonistic fungi from the nutmeg tree to inhibit the development of the cause of the disease. In vitro experiments were carried out by evaluating 4 types of antagonistic fungi from the nutmeg tree to L. theobromae. The aim of the study was to determine the potential of in situ soil fungus as an antagonistic agents against L. theobromae, the cause of dieback disease on the nutmeg tree in Aceh Selatan. Microbial isolation using serial dilution method. The fungus test which was successfully isolated as an antagonist candidate was carried out through the dual culture method. The results showed that four fungi namely; Trichoderma harzianum, T. virens, T. asperellum, and Talaromyces pinophilus have the potential as antagonistic agents against pathogens cause dieback disease on the nutmeg tree in Aceh Selatan, with inhibitory power ranging from 66-100% in vitro.

scholarly journals Antagonistic effectiveness of some bacteria against Fusarium graminearum causing crown rot disease on wheat (Triticum aestivum)

2021 ◽  
Vol 13 (1) ◽  
pp. 69-80
Author(s):  
Majida Hadi Mahdi Alsaady ◽  
Hussein Ali Salim ◽  
Rakib A. Al-ani ◽  
Hadi M. Aboud ◽  
Jamal Talib M Al Roubaie
Keyword(s):  
Fusarium Graminearum ◽  
Culture Method ◽  
Dry Weight ◽  
Antagonistic Effect ◽  
Crown Rot ◽  
Control Treatment ◽  
Enzyme Analysis ◽  
Dual Culture ◽  
Rot Disease

In this study, the antagonistic effect of five bacteria genera namely Bacillus, Pseudomonas, Azotobacter, Azospirillum, and Streptomyces isolated from rhizosphere of wheat were evaluated against Fusarium graminearum as potential biocontrol agents in vitro. F. graminearum was molecularly diagnosed using the Polymerase chain reaction (PCR) technique. Each bacteria were tested for the production of catalase enzyme, oxidase enzyme, analysis of starch, analyze of gelatin, and the motility, where Azotobacter, Azospirillum, and Bacillus subtilis were positive for all tested. Fungal inhibition tests were performed by using the dual culture method and agar well diffusion technique. Among them, Streptomyces and Azospirillum exhibited potent inhibition to the growth of F. graminearum (72.14% and 66.42%) respectively, followed by B.pumillus, P.fluorescens, B. subtilis and Azotobacter ( 58.28%, 43.23%, 39.71% and 35.71%) respectively as compared with the control treatment (0.0%).The dry weight of the fungus biomass was decreased with bacteria P. fluorescens, Streptomyces sp, Azotobacter sp, Azospirillum sp, B. subtilis, and B. pumillus which reached (0.114, 0.103, 0.147, 0.101, 0.143, and 0.107 g) respectively compared to the control treatment that was 0. 665 g.

scholarly journals ASAI KEMAMPUAN BAKTERI ENDOFIT DARI KACANG TANAH DALAM MENGHAMBAT PERTUMBUHAN Sclerotium sp. PADA KECAMBAH KACANG TANAH

2020 ◽  
Vol 14 (2) ◽  
pp. 178-186
Author(s):  
Lisa Novita Arios ◽  
Dwi Suryanto . ◽  
Kiki Nurtjahja . ◽  
Erman Munir .
Keyword(s):  
Endophytic Bacteria ◽  
Culture Method ◽  
Dry Weight ◽  
Dual Culture ◽  
Seedling Height ◽  
Bacterial Isolates ◽  
Peanut Seed ◽  
Number Of Leaves

Assay on ability of endophytic bacteria isolated from peanut to inhibit Sclerotium sp. growth in peanut seedlings.   A study on assay of ability of endophytic bacteria to inhibit Sclerotium sp. in peanut seedling has been done. The bacteria were isolated from peanut healthy plants, while Sclerotium sp. was isolated from infected peanaut plant. Antagonistic assay was conducted by dual culture method.  In vivo assay of inhibiting Sclerotium sp. was conducted by dipping peanut seed in bacterial solution, and planting the seed in soil:compost (3:1) growing media. Six endophytic bacterial isolates showed to inhibit the growth of Sclerotium sp. in vitro. LN1 seemed to inhibit more of Sclerotium sp., while LN5 showed to inhibit less. Two potential isolates LN1 of gram-negative and LN2 of gram-positive using for further study showed to decrease more of dumping off. It also seemed that the isolates increased the seedling height, number of leaves, and dry weight.

scholarly journals ISOLASI DAN UJI ANTAGONIS BAKTERI ASAL TANDAN KOSONG SAWIT YANG DIAPLIKASIKAN PADA AREAL TANAMAN KARET TERHADAP PENYAKIT JAMUR AKAR PUTIH (R. microporus)

2020 ◽  
Vol 4 (2) ◽  
pp. 109-117
Author(s):  
Priyo Adi Nugroho ◽  
Cici Indriani Dalimunthe
Keyword(s):  
Plant Protection ◽  
Culture Method ◽  
Root Disease ◽  
Research Centre ◽  
Dual Culture ◽  
Antagonistic Bacteria ◽  
Isolation And Purification ◽  
Empty Fruit Bunches ◽  
Inhibition Ability

The concern of oil palm empty fruit bunches (OPEFB) utilization in the rubber field that potential to be a host of white root disease needs to be deeply investigated. The objective of this study was to assess the bacteria on the surface of OPEFB that able to inhibit the growth of R. microporus fungi. The antagonistic assay of eight isolates of bacteria against R. microporus fungi was conducted in the Plant Protection Laboratory of Sungei Putih Research Centre. The isolates were isolated from the surface of OPEFB applied in the rubber field. Nutrient agar (NA) was employed in the isolation and purification of bacteria. The antagonistic assay was carried out in vitro using the dual culture method. The observation was performed on 2, 4, 6, and 8 days after incubation (DAI). The result indicated that the eight isolates have varied in inhibition ability of R. Microporus fungi. The initial inhibition was started since 2 DAI particularly in the isolates B2, B3, B4, and B7. The percentage of inhibition were 30%, 60%, 65% dan 69% respectively. Until 8 HSI, the isolates B2, B3, B4, B6, B7, and B8 were still inhibiting the growth of R. microporus fungi > 80%. The isolates B3 and B4 showed the best performance in fungi inhibition with the percentage > 95%. Whereas, the ability of inhibition of the isolates B1 and B5 were very low as < 50%. The existence of such antagonistic bacteria perhaps led to OPEB was not role as the host of white root disease while applied in the rubber field

scholarly journals MEKANISME PARASITISME TRICHODERMA HARZIANUM TERHADAP FUSARIUM OXYSPORUM PADA SEMAI ACACIA MANGIUM

2015 ◽  
Vol 15 (1) ◽  
pp. 72
Author(s):  
Susanti Tasik ◽  
Siti Muslimah Widyastuti ◽  
Harjono .
Keyword(s):  
Fusarium Oxysporum ◽  
Trichoderma Harzianum ◽  
Acacia Mangium ◽  
Culture Method ◽  
Inhibition Mechanism ◽  
Dual Culture ◽  
In Planta ◽  
Know How ◽  
Soil Borne Fungi

Mechanism of parasitism of Trichoderma harzianum on Fusarium oxysporum on Acacia mangium seedlings. Fusarium oxysporum is one of the most important soil-borne fungi the causal agent of damping-off disease. Detailed information it needed to know how the pathogen can be inhibited by Trichoderma harzianum. The objective of this research was to investigate the inhibition mechanism of T. harzianum on F. oxysporum in vitro and in planta. Green Flourescent Protein (GFP) T. harzianum was used as biocontrol agent of F. oxysporum. An in vitro inhibition test of T. harzianum was performed using dual culture method. In the in planta inhibition tests, seedlings of A. mangium were applied with GFP T. harzianum two days before inoculation of F. oxysporum; GFP T. harzianum was simultaneously applied with F. oxysporum and GFP T. harzianum was applied two days after inoculation of F. oxysporum. The inhibition effect of T. harzianum GFP was observed at seven days incubation, indicated by attachment of T. harzianum to F. oxysporum hyphae. GFP T. harzianum hyphae covered the colonies of F. oxysporum at 12 days after incubation. The highest life percentage of A. mangium seedlings was found on the treatment of GFP T. harzianum two days before inoculation of F. oxysporum (82.22%), whereas the lowest life percentage was found on seedling applied with GFP T. harzianum two days after inoculation of F. oxysporum (64.44%).

scholarly journals Morphological and Molecular Characterization of Trichoderma spp. from Rhizosphere Soil and Their Antagonistic Activity against Fusarium spp.

2021 ◽  
pp. 100-112
Author(s):  
Jaygendra Kumar ◽  
Mukesh Kumar ◽  
Akash Tomar ◽  
. Vaishali ◽  
Pushpendra Kumar ◽  
...  
Keyword(s):  
Plant Pathogens ◽  
Rhizosphere Soil ◽  
Pcr Amplification ◽  
Its Region ◽  
Culture Method ◽  
Dual Culture ◽  
Fusarium Spp ◽  
Trichoderma Spp ◽  
Trichoderma Species

Trichoderma species are well known for their biocontrol activity which colonize many soil and tuber-borne and foliage plant pathogens. In this study, 12 native isolates of Trichiderma spp were collected from various crop rhizosphere soil samples and characterized them phenotypically based on morphological and cultural features and genotypically based on sequence analysis of internal transcribed spacer (ITS) region-PCR amplification. The results obtained from phenotypic and genotypic observation revealed that isolates were belonged to five different species namely T. asperellum, T. harzianum, T. longibrachiatum, T. koningii and T. koningiopsis. All Trichoderma isolates produced ~600 bp amplicon and phylogenetic analysis revealed that all isolates were grouped with respective species. Further, the antagonistic potential of all the isolates was evaluated against Fusarium spp. following in vitro dual culture method. The results showed that isolates of T. harzianum exhibited maximum growth inhibition activity. The highest rate of inhibition was recorded with T. harzianum isolate TBT6 (87.1%) followed by TBT7 (82.2%), while the least inhibition was observed in T. longibrachiatum isolate TBT10 (59.7%) after 7 days of incubation. The antagonistic T. harzianum isolate TBT6 can be used for development of Trichoderma based bio-formulation and served as bio-control agent against Fusaium spp. under field conditions.

scholarly journals Antagonisme Fungi Endofit Daun Jarak Pagar (Jatropha curcas) terhadap Fusarium oxysporum C2 Penyebab Busuk Umbi pada Bawang Merah in Vitro

2020 ◽  
Vol 4 (1) ◽  
pp. 47
Author(s):  
Fauziyyah Nahdah ◽  
Noorkomala Sari ◽  
Akhmad Rizali ◽  
Rabiatul Wahdah
Keyword(s):  
Fusarium Oxysporum ◽  
Jatropha Curcas ◽  
Linear Trend ◽  
Culture Method ◽  
Fungal Endophyte ◽  
Dual Culture ◽  
Antimicrobial Substances ◽  
Spss Software

<p class="Abstract">Basal plate rot is a major disease on shallot caused by <em>Fusarium oxysporum</em>. Endophytic fungi is promising to use as antagonist agent to the pathogen. Endophyte is microbes that are living in plant cells and have an asymptomatic characteristic. Nowadays, fungal endophyte is believed to produce antimicrobial substances similar with their plant host's natural product. <em>Jatropha curcas</em> is one of the plants containing secondary metabolites that have antifungal activities. The research aimed to study the ability of endophyte from <em>Jatropha curcas</em> to inhibit the growth of <em>Fusarium oxysporum</em>. The dual culture method was used in this research and the data were analyzed by SPSS software. This antagonism test was conducted by 9 isolates endophyte and each plate consisted of 3 replicates. The result revealed endophyte fungal obtaining 9 isolates with the radial growth of 4,5 cm/2 days. Endophytes of <em>Jatropha curcas</em> L. were able to inhibit the growth of <em>Fusarium oxysporum</em> C2. The percentage of inhibition of <em>Fusarium oxysporum </em>causing of root blight diseases was controlled by up 38.27 - 74.48%. The highest percentage of inhibition is gained by B4b and the lowest of it is A2b. Our observations showed that each endophyte has a consistent linear trend. B4b still leaded as the highest strength to inhibit the growth of pathogen on the monitoring of 3, 5, and 7 days. Moreover, the ability of fungi endophyte from <em>Jatropha curcas</em> as antagonist agent to <em>Fusarium oxysporum</em> needs to be further examined by the in vivo method.</p>

scholarly journals Mikobiota pada Buah Pisang Kultivar Lampung untuk Pengendalian Hayati Fusarium semitectum

2018 ◽  
Vol 14 (1) ◽  
pp. 30
Author(s):  
Okky Setyawati Dharmaputra ◽  
Lisdar Idwan Sudirman ◽  
Evarini Anna Ratnaningsih
Keyword(s):  
Pathogenic Fungus ◽  
Culture Method ◽  
Fruit Rot ◽  
Dilution Method ◽  
Dual Culture ◽  
Postharvest Diseases ◽  
Trichoderma Hamatum ◽  
Fungal Isolates ◽  
Fusarium Semitectum ◽  
Serial Dilution Method

Fusarium semitectum is a pathogenic fungus causing fruit rot of banana fruits. Biological control agents have been used as an alternative to control postharvest diseases. The objective of this study was to test antagonistic potential of mycobiota of banana fruits (Musa cuminata cultivar Lampung) against F. semitectum BIO 91055. The tested fungi were isolated from healthy banana cultivar Lampung collected from Gembrong market located in Bogor using serial dilution method, followed by pour plate method. Test of antagonism activity was carried out using dual culture method. Seventeen fungal isolates were isolated, they consisted of 14 filamentous fungal isolates and 3 yeast isolates. Four filamentous fungal isolates inhibited the growth of F. semitectum BIO 91055 more than 70%, they were Aspergillus niger, Cercosporella sp., Plectosphaerella sp., and Trichoderma hamatum. Three isolates (Cercosporella sp., Plectosphaerella sp., and Trichoderma hamatum) did not cause any diseases of banana fruits and they were considered as potential biocontrol agents.

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