scholarly journals ANALISIS SENYAWA TERPENOID ANTIJAMUR PADA FRAKSI AKTIF EKSTRAK KULIT KAYU CEMPAKA PUTIH (MICHELIA ALBA) DENGAN METODE GAS CHROMATOGRAPHY-MASS SPECTROSCOPY

Jurnal Kimia ◽  
2020 ◽  
pp. 142
Author(s):  
I G. A. G. Bawa ◽  
I G. A. G. C. A. Perbhawa
Keyword(s):  
Gas Chromatography ◽  
Gas Chromatography Mass Spectrometry ◽  
Bark Extract ◽  
Leaf Spot Disease ◽  
Caryophyllene Oxide ◽  
Chromatography Method ◽  
Inhibitory Power ◽  
Gas Chromatography Mass Spectroscopy ◽  
Almost All ◽  
Curvularia Verruculosa

       The bark extract of Michelia alba was able to inhibit the growth of Curvularia verruculosa fungus which was isolated from leaf spot disease in rice. Separation of the active compound was carried out by column chromatography method using the stationary phase of silica gel and the mobile phase of a mixture of n-hexane-acetone (3: 1). Separation of the active extracts resulted in 10 combined fractions, where 4 fractions showed very strong inhibition, namely the HAE fraction (31.50 mm), HAF (30.13), HAG (48.50), and HAH (34.88). The composition of terpenoid compounds in these fractions was analyzed by Gas Chromatography-Mass Spectrometry. The compound ledenoxid (II) and isoaromadendrene epoxide werefound in almost all of the active fractions, while spathulenol wasfound in the HAF and HAG fractions. Other terpenoid compounds found werecis-farnesol, trans-p-mentha-1 (7), 8-dien-2-ol, and neoclovenoxid-alcohol in the HAE fraction, (-)-caryophyllene oxide and tomentosin in the fraction of HAG. All of the detected terpenoid compounds were compounds from the sesquiterpene group. Keywords: Michelia alba extract, inhibitory power, Curvularia verruculossa, sesquiterpenes

scholarly journals Identification Michelia alba barks extract using Gas Chromatography-Mass Spectrometry (GC-MS) and its antifungal properties to inhibit microbial growth

2020 ◽  
Vol 21 (4) ◽  
Author(s):  
I Made Dira Swantara ◽  
I Gusti Agung Gede Bawa ◽  
Dewa Ngurah Suprapta ◽  
Kadek Karang Agustina ◽  
I Gede Rai Maya Temaja
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Leaf Spot ◽  
Microbial Growth ◽  
Chemical Compounds ◽  
Gas Chromatography Mass Spectrometry ◽  
Bark Extract ◽  
Leaf Spot Disease ◽  
Spot Disease ◽  
Antifungal Properties

Abstract. Swantara IMD, Bawa IGAG, Suprapta DN, Agustina KK, Temaja IGRM. 2020. Identification Michelia alba barks extract using Gas Chromatography-Mass Spectrometry (GC-MS) and its antifungal properties to inhibit microbial growth. Biodiversitas 21: 1541-1550. Fungicides are substances that inhibit the growth or kill the pathogenic fungi. A substance can be categorized as a fungicide if it shows antifungal activity. This activity is resulting from bioactive compounds derived from the secondary metabolites. Michelia alba bark’s extract could inhibit the growth of Curvularia verruculosa, the cause of leaf spot disease on rice. The purpose of this study was to analyze chemical compounds of bark extract which inhibit the growth of C. verruculosa. The extraction of bark metabolites of M. alba was done using methanol as the solvent. The inhibitory test of the extract was carried out by the diffusion well method on Potato Dextrose Agar (PDA) medium. Analysis of chemical compounds of the M. alba extract was carried out in Gas chromatography-mass spectroscopy. The results of inhibitory test found that the M alba bark extract inhibits the C. verruculosa with the inhibition zone as wide as 36 mm in diameter. The majority of chemical compounds identified from the M. alba extract consisted of 10 compounds, namely: Hexadecanoic acid, methyl ester; 4H-tomentosine; 4H-tomentosine (compound isomer 2); 3-hydroxy pregn-4-ene-20-one; Tomentosine; 2-hydroxy tomentosine; Tert-butyl-2-aminophenylcarbamol; 2H-cyclohepta [b] furan-2-one, 3,3a, 4,7,8,8a-hexahydro-7-methyl-3-methylene-6-(2-formil-3-oxobutyl)-; Isoxanthanol and Xanthanol. To conclude, this extract is useful to treat C. verruculosa that causes leaf spot disease on rice.

scholarly journals Chemical Composition of the Essential Oil of Croton bonplandianus from India

2014 ◽  
Vol 9 (2) ◽  
pp. 1934578X1400900
Author(s):  
Rajesh K. Joshi
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Chemical Composition ◽  
Essential Oil ◽  
Gas Chromatography Mass Spectrometry ◽  
Caryophyllene Oxide ◽  
Aerial Parts ◽  
Sesquiterpene Hydrocarbons ◽  
Germacrene D ◽  
Total Oil

The essential oil obtained from the aerial parts of Croton bonplandianus Baill. was analyzed by gas chromatography (GC) and gas chromatography/mass spectrometry (GC/MS). A total of 37 compounds have been identified, representing 96.2% of the total oil. The main constituents were identified as β-caryophyllene (16.7%), germacrene D (14.7%), borneol (8.3%), Z-β-damascenone (6.(%), isobornyl acetate (6.2%), α-humulene (6.1%), germacrene A (5.2%) and caryophyllene oxide (4.5%). The oil was rich in sesquiterpene hydrocarbons (60.1%).

scholarly journals Chemical Composition and Acaricidal Activity of Nemuaron vieillardii Essential Oil against the Cattle Tick Rhipicephalus (Boophilus) microplus

2016 ◽  
Vol 11 (12) ◽  
pp. 1934578X1601101
Author(s):  
Nicolas Lebouvier ◽  
Thomas Hue ◽  
Joseph Brophy ◽  
Edouard Hnawia ◽  
Mohammed Nour
Keyword(s):  
Gas Chromatography ◽  
Chemical Composition ◽  
Essential Oil ◽  
Tick Control ◽  
Acaricidal Activity ◽  
Boophilus Microplus ◽  
Gas Chromatography Mass Spectrometry ◽  
Cattle Tick ◽  
Caryophyllene Oxide ◽  
Human Carcinogen

Essential oil from leaves of Nemuaron vieillardii (Baill.) Baill., a shrub used in the kanak pharmacopeia, was analyzed by gas chromatography and combined gas chromatography-mass spectrometry. The main compounds identified were safrole (49.7%), linalool (8.0%), δ-cadinene (5.1%), caryophyllene oxide (4%) and α-copaene (2.4%). This chemical composition is consistent with leaf oils of the Atherospermataceae family and demonstrates the proximity of the two genera Nemuaron and Atherosperma as regards to their essential oil compositions. The modified Larval Packet Test (LPT) was used to assess acaricidal effect of N. vieillardii essential oil on larvae of the cattle tick Rhipicephalus (Boophilus) microplus and LC50 was then calculated to 14.67%. High content of safrole in this oil which is suspected of being a human carcinogen, poses the problem of the use of this oil in a perspective of development of alternative tick control strategy and in the traditional medicinal consumption of Nemuaron vieillardii.

scholarly journals Chemical investigation of the essential oil of Laggera crispata (Vahl) Hepper & Wood from India

2011 ◽  
Vol 76 (4) ◽  
pp. 523-528 ◽  
Author(s):  
Ram Verma ◽  
Rajendra Padalia ◽  
Chandan Chanotiya ◽  
Amit Chauhan ◽  
Anju Yadav
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Essential Oil ◽  
Chemical Investigation ◽  
Gas Chromatography Mass Spectrometry ◽  
Western Himalayas ◽  
Caryophyllene Oxide ◽  
Oil Composition ◽  
Aerial Parts ◽  
Total Oil

Hydrodistilled essential oil of the aerial parts of Laggera crispata (Vahl) Hepper & Wood, collected from the Kumaon region of the western Himalayas was analysed by gas chromatography and gas chromatography-Mass Spectrometry. Eighty constituents, accounting for 83.9 % of the total oil composition, were identified. The oil was mainly dominated by sesquiterpenoids (45.3 %) and benzenoid compounds (33.9 %). Among them, 2,5-dimethoxy-p-cymene (32.2 %), 10-epi-?-eudesmol (14.7 %), ?-caryophyllene (6.9 %), and caryophyllene oxide (5.4 %) were major components of the oil.

scholarly journals Constituents and Anthelmintic Activity Evaluation of Albizia Adiantifolia (Schumach) W.F. Wright Essential Oils FromNigeria

2018 ◽  
Vol 10 (2) ◽  
pp. 10 ◽  
Author(s):  
Akinsola Akande ◽  
Sherifat Aboaba ◽  
Guido Flamini
Keyword(s):  
Gas Chromatography ◽  
Essential Oils ◽  
Anthelmintic Activity ◽  
Stem Bark ◽  
Mass Spectrometry Analysis ◽  
Gas Chromatography Mass Spectrometry ◽  
Root Bark ◽  
Dependent Manner ◽  
Caryophyllene Oxide ◽  
Significant Difference

Albizia adiantifolia (Schumach) W.F. Wright (Fabaceae) is a plant used traditionally in treating different health ailments which includes worm infections. The essential oils (EOs) were obtained by hydrodistillation in an all glass Clevenger apparatus, and characterized by gas chromatography (GC) and gas chromatography-mass spectrometry analysis (GC-MS). In vitro petri-dish anthelmintic activity was carried out using adult earthworm, Eudrilus eugeniae. The leaves, stem bark and root bark EOs afforded a total of 40, 26 and 20 constituents representing 90.9%, 94.1% and 90.9% of the total oil fractions respectively. The classes of compounds identified in the leaves, stem bark and root bark are oxygenated monoterpenes (4.1%, 1.7% and 4.0% respectively), sesquiterpene hydrocarbons (39.5%, 67.3% and 42.6% respectively), oxygenated sesquiterpenes (18.7%, 22.3% and 30.1% respectively), non-terpene derivatives (12.1%, 2.6% and 14.2% respectively) and apocarotenoids (16.5% and 0.2% in the leaves and stem-bark). β-caryophyllene (23.0%), E-geranylacetone (7.4%), acorenone (6.4%), viridiflorol (6.4%), α-zingiberene (6.3%) and ar-curcumene (4.6%) were the major constituents in the leaves oil, β-caryophyllene (39.3%), selin-11-en-4-α-ol (10.4%), α-zingiberene (9.6%), ar-curcumene (7.2%), caryophyllene oxide (6.4%) and α-humulene (5.6%) were the major constituents in the stem bark oil, while β-caryophyllene (32.1%), selin-11-en-4-α-ol (13.1%), caryophyllene oxide (8.4%), pentadecanal (6.1%) and α -humulene (4.4%) were the major constituents in the root bark oil. β -caryophyllene dominated the oils. The leaf EO was the most active against E. eugeniae worm. All the oils showed a relatively higher activity compared to Albendazole, in a concentration dependent manner. There was significant difference (p<0.001) in activity.

Comparative Analysis of Essential Oil Components of Two Pinus Species from Taibai Mountain in China

2010 ◽  
Vol 5 (8) ◽  
pp. 1934578X1000500
Author(s):  
Yuan Zhang ◽  
Zhezhi Wang
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Comparative Analysis ◽  
Essential Oil ◽  
Gas Chromatography Mass Spectrometry ◽  
Caryophyllene Oxide ◽  
Pinus Species ◽  
Taibai Mountain ◽  
Oil Components ◽  
Optimum Extraction

Gas chromatography (GC) and gas chromatography–mass spectrometry (GC–MS) were used to compare between the essential oil components from needles of Pinus armandii Franch versus P. tabulaeformis Carr., growing on the same site at Taibai Mountain, China. Under optimum extraction and analysis conditions, 65 and 66 constituents each were identified in P. armandii and P. tabulaeformis, which accounted for 87.9% and 87.1%, respectively, of their oils. Based on their terpene compositions, we concluded that these species belong to a high-caryophyllene chemotype, with sesquiterpenes comprising 54.4% to 54.8% of the total contents. We also determined minor qualitative and major quantitative variations in some compounds. Compared with that from P. tabulaeformis, P. armandii oil had more γ-muurolene (7.5%), terpinolene (5.8%), and longifolene (5.7%). In contrast, α-pinene (8.6%) and caryophyllene oxide (7.4%) were the dominant compounds in P. tabulaeformis.

scholarly journals IDENTIFICATION OF BIOACTIVE COMPONENTS IN ENHALUS ACOROIDES SEAGRASS EXTRACT BY GAS CHROMATOGRAPHY–MASS SPECTROMETRY

2018 ◽  
Vol 11 (10) ◽  
pp. 313 ◽  
Author(s):  
Amudha P ◽  
Jayalakshmi M ◽  
Pushpabharathi N ◽  
Vanitha V
Keyword(s):  
Gas Chromatography ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Antioxidant Property ◽  
Butylated Hydroxytoluene ◽  
Gas Chromatography Mass Spectrometry ◽  
Antitumor Activities ◽  
Enhalus Acoroides ◽  
Gas Chromatography Mass Spectroscopy

Objective: This study deals with the determination of possible phytocompounds present in the ethyl acetate extract of Enhalus acoroides using gas chromatography-mass spectroscopy (GC-MS) technique. Methods: Using GC-MS technique the phytocompounds present in the ethyl acetate extract of E. acoroides whole seagrass was investigated, and the mass spectra of the compounds found in the extract were matched with the National Institute of Standards and Technology library.Results: GC-MS analysis of E. acoroides extract revealed the existence of several phytocompounds which includes 1-nonadecene (17.15%), n-tetracosanol-1 (11.48%), 1-octadecene (10.06%), 2-pentadecanone (7.87%), behenyl alcohol (7.33%), 17-pentatriacontene (4.84%), triacontane (4.25%), tetratetracontane (4.17%), and butylated hydroxytoluene (2.09%).Conclusion: E. acoroides possess distinct phytocompounds such as 1-nonadecene and n-tetracosanol-1 which possess antioxidant property, triacontane which has antibacterial, antidiabetic and antitumor activities. Further studies need to elute novel bioactive compounds and toxicity profile through animal models. 

scholarly journals Chemical Composition of the Essential Oil of Baccharoides lilacina from India

2013 ◽  
Vol 8 (3) ◽  
pp. 1934578X1300800
Author(s):  
Rajesh K. Joshi
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Essential Oil ◽  
Essential Oil Composition ◽  
Gas Chromatography Mass Spectrometry ◽  
Caryophyllene Oxide ◽  
Oil Composition ◽  
Aerial Parts ◽  
Sesquiterpene Hydrocarbons ◽  
Total Oil

The essential oil composition from the aerial parts of Baccharoides lilacina (Dalzell & A. Gibson) M. R. Almeida was analyzed by gas chromatography (GC) and gas chromatography/mass spectrometry (GC/MS). A total of 41 compounds have been identified, representing 97.4% of the total oil. The main constituents were identified as β-caryophyllene (27.7%), epi-α-cadinol (25.1%), caryophyllene oxide (9.9%), α-muurolol (7.6%), α-cadinene (6.1%) and α-cadinol 4.5%). The oil was found to be rich in oxygenated sesquiterpenes (47.1%) and sesquiterpene hydrocarbons (46.2%).

scholarly journals CHEMICAL COMPOSITION OF THE ESSENTIAL OIL FROM LEAVES OF CALLICARPA ARBOREA ROXB. GROWING IN VIETNAM

2021 ◽  
Vol 58 (6A) ◽  
pp. 280
Author(s):  
Hung Huy Nguyen ◽  
Dai Ngoc Do ◽  
Prabodh Satyal ◽  
Chung Thanh Nguyen ◽  
Nguyen Van Bui ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Chemical Composition ◽  
Essential Oil ◽  
Gas Chromatography Mass Spectrometry ◽  
Caryophyllene Oxide ◽  
Chromatography Mass Spectrometry ◽  
First Time

The essential oil from the leaves of Callicarpa arborea Roxb. growing wild in Vietnam, was obtained by hydrodistillation and analyzed by gas chromatography – mass spectrometry. The major constituents of leaves of C. arborea were β-selinene (23.9%), ar-turmerone (17.5%) and α-copaene (8.9%), with lesser concentrations of caryophyllene oxide (4.9%) and ar-turmerol (3.9%). The chemical composition of this essential oil sample is being reported for the first time.

scholarly journals GAS CHROMATOGRAPHY-MASS SPECTROSCOPY STUDIES ON ETHANOLIC EXTRACT OF DRIED LEAVES OF CATHARANTHUS ROSEUS

2018 ◽  
Vol 11 (6) ◽  
pp. 336 ◽  
Author(s):  
Gaurav M Doshi ◽  
Bernadette D Matthews ◽  
Pratip K Chaskar
Keyword(s):  
Gas Chromatography ◽  
Ethyl Ester ◽  
Catharanthus Roseus ◽  
Research Work ◽  
Acid Ethyl Ester ◽  
Ethanolic Extract ◽  
Soxhlet Extraction ◽  
Gas Chromatography Mass Spectrometry ◽  
Molecular Formula ◽  
Gas Chromatography Mass Spectroscopy

Objective: Phytochemical screening of the ethanolic extract of Catharanthus roseus disclosed the presence of alkaloids, terpenoids, phenols, tannins, saponins, quinines, flavonoids, and proteins. In the present research work, we have identified and confirmed the structures of the constituents present by means of a hyphenated technique of gas chromatography-mass spectrometry (GC-MS) from the extract.Method: Shade-dried leaves of C. roseus were powdered and extracted by means of Soxhlet extraction using ethanol as a solvent. Crude extract obtained was utilized for GC-MS.Results: A total of 15 components were identified, some of which were n-hexadecanoic acid, ethyl ester, Vitamin E, 9,12,15-octadecatrienoic acid,2- [(trimethylsilyl)oxy]-1-[{(trimethylsilyl)oxy}methyl]ethyl ester, phytol, 3-epivindolinine, and oleic acid.Conclusion: GC-MS studies helped us to assess the phytochemical constituents based on retention time, molecular formula, molecular weight and the corroboration of MS libraries. In future, C. roseus leaves may be subjected to diverse types of extraction methodologies, and a number of various elucidated phytoconstituents can be studied for their ethnopharmacological significance and applicability citing literature.

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