scholarly journals Screening the Milieu of an abattoir for Bacteria of Public health importance in Makurdi, Benue State, Nigeria

2017 ◽  
Vol 7 (2) ◽  
pp. 1
Author(s):  
Nseabasi Maina N ◽  
E. G Vinkings ◽  
I U Bassey ◽  
A. A Unimke ◽  
L.O Abulawor
Keyword(s):  
Public Health ◽  
Escherichia Coli ◽  
Nalidixic Acid ◽  
Antibiotic Sensitivity ◽  
Soil Samples ◽  
Bacillus Species ◽  
Bacterial Cells ◽  
Pseudomonas Sp ◽  
Bacillus Sp ◽  
Public Health Importance

The study investigated the bacteriological content in a major abattoir located approximately 300 meters from a densely populated and cultivated area along the Benue River. Enumeration of bacterial cells from samples in the study yielded relatively high mean count of 9.4 x 105 and 7.3 x 105 from effluent and soil samples respectively. Bacteria isolated from both samples included; Escherichia coli, Streptococcus sp, Salmonella sp, Pseudomonas sp, Shigellasp, Enterobacter sp, Staphylococcus sp, Bacillus sp, Brucella sp, Proteus sp, Micrococcus sp etc. Escherichia coli recorded an occurrence of 18.53% in effluents and 16.16% in soil while Proteus species and Brucella sp had an occurrence of 9.59% and 1.39% respectively in soil samples. Antibiotic sensitivity screening using seveenteen (17) antibiotics disc (Optun Nig.) viz: Tarivid (10 µg), peflacine (10 µg), Agumentin (30 µg), Gentamycin (10 µg), Streptomycin (30 µg), Ceporex (10 µg), Nalidixic acid (30 µg), Ciprofloxacin (10 µg), Norfloxacin (10 µg), Rifampicin (µg), Erythromycin (µg), Chloramphenicol (µg), Ampiclox (30 µg), Levofloxacin (10µg). A marked level of resistance was observed among the isolates. However, Escherichia coli indicated sensitivity to peflacine, Shigellasp indicated sensitivity to augmentin, Enterococcus and Bacillus species indicated sensitivity to ciprofloxacin and streptomycin respectively.

scholarly journals Bacteria Associated with Petroleum Hydrocarbon Contaminated Soil from NNPC Depot

2020 ◽  
pp. 77-82
Author(s):  
S. O. Bankole ◽  
M. B. Oyedeji ◽  
F. O. Akanni ◽  
E. P. Chukwudebe ◽  
A. O. Olatunji ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Local Government ◽  
Contaminated Soil ◽  
Petroleum Hydrocarbon ◽  
Research Work ◽  
Soil Samples ◽  
Pseudomonas Sp ◽  
Bacillus Sp ◽  
Plate Method ◽  
Pseudomonas Species

The microorganisms associated with soil polluted with petroleum hydrocarbon were isolated at NNPC depot Ibadan, in Ido Local Government Areas of Oyo State. Soil samples were taken from three different points along the point of discharge. The spread plate method was used to isolate the microorganisms found in these rivers and were later identified. From the research, seven different species of microorganism were isolated, which are Pseudomonas sp, Bacillus sp., Micrococcus sp. Staphylococcus aureaus sp., Enterobacter sp., Klebsiella sp. and Escherichia coli. Pseudomonas species were found to be more prominent in the samples collected in a research work. This suggested that the isolates are resistant to the petroleum hydrocarbon and can be recommended as candidates for the clean-up of petroleum hydrocarbon contaminated soil.

scholarly journals Studies on decolorization of textile dye by using Pseudomonas and bacillus sp from the contaminated effluent soil samples of Kovilpatti, Thoothukudi district of Tamil Nadu

2019 ◽  
Vol 11 (1) ◽  
pp. 134-137 ◽  
Author(s):  
Kannan D ◽  
Renuga Devi ◽  
A. G. Murugesan ◽  
S. Rajan
Keyword(s):  
Contaminated Soil ◽  
Tamil Nadu ◽  
Soil Samples ◽  
Toxic Chemicals ◽  
Textile Dye ◽  
Pseudomonas Sp ◽  
Bacillus Sp ◽  
Bacterial Strains ◽  
Textile Industries ◽  
Source Of Pollution

Textile industries releasing large amount of effluent which contains textile dyes and toxic chemicals and it is one of the major source of pollution also contaminating water bodies. To remove that, bacteria have been of great attention because of their ability to treat effluent. The present study was undertaken to exploit the ability of Pseudomonassp and Bacillus sp from dye contaminated soil samples for bioremediation for dye effluent. Among the bacterial strains used in the study. Pseudomonas sp emerged out to be most potent decolorizer in comparison to Bacillus sp with the degree of decolorization of 90.0 %. Thus, it was concluded that the Pseudomonas sp had highest color removing capacity from contaminated effluent soil samples. 

scholarly journals Pola Kuman Penyebab Ventilator Associated Pneumonia(VAP) dan Sensitivitas Terhadap Antibiotik di RSAB Harapan Kita

Sari Pediatri ◽  
2016 ◽  
Vol 13 (6) ◽  
pp. 384
Author(s):  
Retno Widyaningsih ◽  
Latre Buntaran
Keyword(s):  
Escherichia Coli ◽  
Pseudomonas Aeruginosa ◽  
Stenotrophomonas Maltophilia ◽  
Clinical Laboratory ◽  
Enterobacter Aerogenes ◽  
National Committee ◽  
Klebsiella Pneumonia ◽  
Ventilator Associated Pneumonia ◽  
Pseudomonas Sp ◽  
Bacillus Sp

Latar belakang. Peningkatan resistensi antibiotik di antara bakteri penyebab pneumonia nosokomial yang didapat di rumah sakit telah banyak dilaporkan. Jika kita tidak mengenal pola kepekaan kuman di suatu rumah sakit akan menyulitkan pemberian terapi empiris awal. Tujuan. Mengetahui profil kuman penyebab pneumonia yang didapat di rumah sakit pada anak serta uji sensitivitas terhadap beberapa antibiotik.Metode. Studi deskriptif retrospektif dengan sumber data yang berasal dari rekam medis Laboratorium Mikrobiologi RSAB Harapan Kita periode Januari hingga Juni 2010. Spesimen adalah semua spesimen saluran respiratorik dari pasien dengan diagnosis pneumonia yang dirawat. Biakan dan uji resistensi dilakukan menurut standar National Committee for Clinical Laboratory Standards(NCCLS).Hasil. Didapatkan 116 spesimen biakan dan di antaranya 4 (3,4%) steril. Dari 112 biakan positif, 79.5% di antaranya adalah bakteri gram negatif berturut-turut dari yang paling dominan adalah Pseudomonas sp.(22,4%), Pseudomonas aeruginosa(18,1%), Stenotrophomonas maltophilia(9.5%), Serratia marcescens(8,6%),Enterobacter aerogenes(7,8%), Klebsiella pneumonia, Bacillus sp., dan Escherichia coli(masing-masing 5,2%). Golongan Pseudomonasmemiliki sensitivitas terhadap ceftazidime, amikacin serta netilmicin.Kesimpulan. Basil gram negatif aerob (79,5%) merupakan mikroorganisme penyebab yang paling dominan. Ceftazidime, diikuti terhadap amikacin serta netilmicin masih mempunyai sensitivitas yang tinggi sehingga dapat dipakai sebagai terapi awal VAP.

scholarly journals ISOLATION AND CHARACTERIZATION OF ESCHERICHIA COLI FROM BUFFALO CALVES IN SOME SELECTED AREAS OF BANGLADESH

1970 ◽  
Vol 8 (1) ◽  
pp. 23-26 ◽  
Author(s):  
SK Paul ◽  
MSR Khan ◽  
MA Rashid ◽  
J Hassan ◽  
SMS Mahmud
Keyword(s):  
Escherichia Coli ◽  
Nalidixic Acid ◽  
Rectal Swab ◽  
Antibiotic Sensitivity ◽  
Buffalo Calves ◽  
E Coli ◽  
Isolation And Characterization ◽  
Sensitivity Pattern ◽  
Highly Sensitive

The research works was conducted with a view to isolate and identify the Escherichia coli (E. coli) organism from diarrhoeic cases of buffalo reared in selected areas of Bangladesh as well the prevalence and antibiotic sensitivity pattern of the isolated E. coli in the Department of Microbiology and Hygiene, Bangladesh Agricultural University (BAU), Mymensingh-2202 during the period from April 2008 to May 2009. A total of 50 rectal swab samples were collected from 4 different places namely Haluaghat and Boira of Mymensingh, Madupur of Tangail and Kazipur of Sirajgonj districts. The samples were aseptically carried to the laboratory of the Department of Microbiology and Hygiene and subjected to different cultural, morphological and biochemical examinations. Upon cultural, morphological and biochemical examinations 23 (45%) samples were found to be positive for E. coli. The highest prevalence was found in Haluaghat, Mymensingh (53.33%) and the lowest (40.00%) in Boira, Mymensingh and Kazipur, Sirajganj. Antibiogram study revealed that the isolated E. coli was highly sensitive to Enrofloxacin and Ciprofloxacin, moderately sensitive to Cefalexin and Amoxicillin, and resistant to Nalidixic acid and Erythromycin. DOI = 10.3329/bjvm.v8i1.7398 Bangl. J. Vet. Med. (2010). 8(1): 23-26

scholarly journals Isolasi dan Aktivitas Antibakteri Aktinomisetes Asal Tanah Gambut Riau

2018 ◽  
Vol 10 (1) ◽  
pp. 18
Author(s):  
Tetty Marta Linda ◽  
Rodesia Mustika Roza ◽  
Rola Yuliati ◽  
Wahyuliyanti Wahyuliyanti
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Bacillus Subtilis ◽  
Peat Soil ◽  
Soil Samples ◽  
Pseudomonas Sp ◽  
Plate Method ◽  
E Coli ◽  
Gram Positive Bacteria ◽  
And Staphylococcus Aureus

The aims of this study are to isolate actinomycetes from peat soil samples, to determine the ability of actinomycetes to inhibit the growth of Gram positive bacteria (Bacillus subtilis and Staphylococcus aureus) and Gram negativebacteria (Escherichia coli and Pseudomonas sp.). A total of 14 actinomycetes strains were recovered from peat soil samples using pour plate method with Starch Casein Agar. The results showed that 11 isolates were active against B. subtilis, 8 isolates against S. aureus, 8 isolates were active against E. coli and 8 isolates againstPseudomonas sp. Two isolates (SM 1.3 and SM 1.6) were active against all bacterial targets.

scholarly journals Enhanced Biodegradation of Degreaser Using Pseudomonas and Bacillus Species in Fresh Water Ecosystem

2019 ◽  
pp. 1-10
Author(s):  
Renner Renner Nrior ◽  
Indutimi Mathias Otuogha
Keyword(s):  
Fresh Water ◽  
Heterotrophic Bacteria ◽  
Bacillus Species ◽  
Freshwater Ecosystem ◽  
Pseudomonas Sp ◽  
Bacillus Sp ◽  
Microbiological Methods ◽  
Pseudomonas Species ◽  
Set Up ◽  
Hydrocarbon Utilizing Bacteria

Aim: The aim of this study is to enhance the biodegradation of degreasers used in upstream sectors of Nigeria Petroleum Industry using bio-augmenting organisms such as: Pseudomonas and Bacillus species in freshwater Ecosystem. Study Design: This study employs experimental designs, Randomized Block Design treatment set up, statistical analysis of data and interpretation. Place and Duration of Study: Freshwater sample for this research was collected from Asarama Andoni, in Rivers State, Nigeria. The study lasted for six months. Methodology: The experimental set-up was carried in 500 ml conical flask with two species of bacteria, two types of degreaser and fresh water sample giving a total of 8 set-up including controls. The Pseudomonas and Bacillus species used in this study were isolated from the freshwater ecosystem and identified using standard microbiological methods. The bioremediation potential of the respective test organisms were monitored at ambient temperature 28±0.2°C for 28 days at a constant interval of 7 days using the following Physiochemical parameter; Total dissolved Solid, Hydrogen concentrations ions and Total Hydrocarbon Content. While the following Microbiological parameters; Total heterotrophic Bacteria, Total Heterotrophic Fungi, Hydrocarbon Utilizing Bacteria, and Hydrocarbon Utilizing Fungi were monitored. Results: The percentage of degradability of the respective set-ups ranged from Control (Rigwash) (3.29%) < Pseudomonas sp. + Rigwash (27.56%) < Pseudomonas + Bacillus + Rigwash< (31.57%), Bacillus sp.+ Rigwash (37.57%) Control 2 (Aquabreak) (9.45%) < Pseudomonas sp.+ Aquabreak (26.77%) < Pseudomonas + Bacillus + Aquabreak (31.32%)< Bacillus sp.+ Aquabreak (32.46%). Overall evaluation revealed that Bacillus sp. had a higher biodegradation potential on both degreaser (Rigwash and Aquabreak) in freshwater than Pseudomonas sp. Five species of bacteria: Escherichia coli, Micrococcus, Citrobacter, Bacillus, and Pseudomonas species and four fungal species: Penicillium, Mucor, Aspergillus and Rhizopus species were isolated and identified as hydrocarbon utilizing bacteria and fungi organisms respectively. Conclusion: The results revealed that Bacillus species have more degradability potential than Pseudomonas species for both Aquabreak and Rigwash. These results also indicated the low biodegradation potential of Rigwash in fresh Ecosystem.

scholarly journals Pathogenic bacteria and parasites of human importance from cow dung

2021 ◽  
Vol 6 (1) ◽  
pp. 37-38
Author(s):  
Chourasiya R ◽  
Bhattacharyya S ◽  
Dighe P ◽  
Banik A ◽  
Raj A
Keyword(s):  
Public Health ◽  
Escherichia Coli ◽  
Entamoeba Histolytica ◽  
Pathogenic Bacteria ◽  
Microbial Flora ◽  
Cow Dung ◽  
Public Health Importance ◽  
Cryptosporidium Spp ◽  
Available Information

Cow dung is used commonly in rural and periurban households in India for many purposes like for cooking and mopping floors. However it can contain many pathogenic bacteria and parasites like Escherichia coli, Cryptosporidium spp. and Entamoeba histolytica that can be infectious to the handlers and farmers. Hence one should be very careful while handling these. Thus a study of pathogenic microbial flora in cow dung is of utmost public health importance in our country and very little literature is available regard it. Hence, we here have tried to summarize available information in this aspect.

scholarly journals Identification of pBC218/pBC210 Genes of Bacillus cereus G9241 in Five Florida Soils Using qPCR

2014 ◽  
Vol 2014 ◽  
pp. 1-7
Author(s):  
Vicki Ann Luna ◽  
Kimmy Nguyen ◽  
Damian H. Gilling
Keyword(s):  
Public Health ◽  
Bacterial Species ◽  
Geographical Area ◽  
Soil Samples ◽  
Open Reading Frames ◽  
Public Health Importance ◽  
Potential Threat ◽  
Gram Negative ◽  
Positive Results ◽  
Reading Frames

The distribution of the virulent plasmid pBC210 of B. cereus that carries several B. anthracis genes and has been implicated in lethal anthrax-like pulmonary disease is unknown. We screened our collection of 103 B. cereus isolates and 256 soil samples using a quantitative PCR (qPCR) assay that targeted three open reading frames putatively unique to pBC210. When tested with DNA from 2 B. cereus strains carrying pBC210, and 64 Gram-positive and 55 Gram-negative bacterial species, the assay had 100% sensitivity and specificity. None of the DNA from the B. cereus isolates yielded positive amplicons but DNA extracted from five soils collected in Florida gave positive results for all three target sequences of pBC210. While screening confirms that pBC210 is uncommon in B. cereus, this study is the first to report that pBC210 is present in Florida soils. This study improves our knowledge of the distribution of pBC210 in soils and, of public health importance, the potential threat of B. cereus isolates carrying the toxin-carrying plasmid. We demonstrated that sequences of pBC210 can be found in a larger geographical area than previously thought and that finding more B. cereus carrying the virulent plasmid is a possibility in the future.

scholarly journals Evolution of Resistance to Continuously Increasing Streptomycin Concentrations in Populations of Escherichia coli

2015 ◽  
Vol 60 (3) ◽  
pp. 1336-1342 ◽  
Author(s):  
Fabrizio Spagnolo ◽  
Conrad Rinaldi ◽  
Dannah Rae Sajorda ◽  
Daniel E. Dykhuizen
Keyword(s):  
Escherichia Coli ◽  
High Frequency ◽  
Bacterial Resistance ◽  
Low Frequency ◽  
Antibiotic Sensitivity ◽  
Modern Medicine ◽  
Bacterial Cells ◽  
Modern Biology ◽  
Content Type ◽  
Evolution Of Resistance

The evolution of antibiotic resistance in bacteria has become one of the defining problems in modern biology. Bacterial resistance to antimicrobial therapy threatens to eliminate one of the pillars of the practice of modern medicine. Yet, in spite of the importance of this problem, only recently have the dynamics of the shift from antibiotic sensitivity to resistance in a bacterial population been studied. In this study, a novel chemostat method was used to observe the evolution of resistance to streptomycin in a sensitive population ofEscherichia coli, which grew while the concentration of antibiotic was constantly increasing. The results indicate that resistant mutants remain at a low frequency for longer than expected and do not begin to rise to a high frequency until the antibiotic concentrations are above the measured MIC, creating a “lull period” in which there were few bacterial cells growing in the chemostats. Overall, mutants resistant to streptomycin were found in >60% of the experimental trial replicates. All of the mutants detected were found to have MICs far above the maximum levels of streptomycin to which they were exposed and reached a high frequency within 96 h.

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