scholarly journals Licorice Root Ethanol Extract Induces Cell Proliferation in Human Osteoblast Cells

2021 ◽  
Vol 9 (4) ◽  
pp. 803-806
Author(s):  
Sema Misir
Keyword(s):  
Cell Proliferation ◽  
Biological Effects ◽  
Periodontal Regeneration ◽  
Study Groups ◽  
Licorice Root ◽  
Root Extract ◽  
Human Osteoblast ◽  
Osteoblast Cells ◽  
Proliferative Effect ◽  
Licorice Root Extract

Licorice, also known as the root of Glycyrrhiza glabra, has been used for many years in traditional medicine to treat various diseases. Licorice root has remarkable pharmacological properties and these biological effects are predominantly attributed to its content of polyphenols and flavonoids. The aim of this study was to determine the proliferative effect of licorice root extract on human osteoblast cells. The study groups were exposed to various concentrations of licorice root extract on 31.25, 62.5, 250, 500, 1000 μg/mL for 24, and 48 h. The proliferative effect of the extract on human osteoblast cells was assessed using the MTT assay. After 24 and 48 h, cell proliferation of groups treated were increased statistically significant compared to the control cells, and also all concentrations showing no cytotoxic effects on osteoblast cells. Phytomedical applications of licorice root may represent a promising approach in the treatment of periodontal regeneration and osteoporosis.

Icariin Stimulates hFOB 1.19 Osteoblast Proliferation and Differentiation via OPG/RANKL Mediated by the Estrogen Receptor

2020 ◽  
Vol 22 (1) ◽  
pp. 168-175 ◽  
Author(s):  
Lin-Jun Sun ◽  
Chong Li ◽  
Xiang-hao Wen ◽  
Lu Guo ◽  
Zi-Fen Guo ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Estrogen Receptor ◽  
Protein Expression ◽  
Chinese Herb ◽  
Human Osteoblast ◽  
Osteoblast Cells ◽  
Expression Ratio ◽  
Cell Proliferation And Differentiation ◽  
Proliferation And Differentiation ◽  
Mrna And Protein Expression

Background:: Icariin (ICA), one of the main effective components isolated from the traditional Chinese herb Epimedium brevicornu Maxim., has been reported to possess extensive pharmacological actions, including enhanced sexual function, immune regulation, anti-inflammation, and antiosteoporosis. Methods:: Our study was designed to investigate the effect of ICA on cell proliferation and differentiation and the molecular mechanism of OPG/RANKL mediated by the Estrogen Receptor (ER) in hFOB1.19 human osteoblast cells. Results:: The experimental results show that ICA can stimulate cell proliferation and increase the activity of Alkaline Phosphatase (ALP), Osteocalcin (BGP) and I Collagen (Col I) and a number of calcified nodules. Furthermore, the mRNA and protein expression of OPG and RANKL and the OPG/ RANKL mRNA and protein expression ratios were upregulated by ICA. The above-mentioned results indicated that the optimal concentration of ICA for stimulating osteogenesis was 50ng/mL. Subsequent mechanistic studies comparing 50ng/mL ICA with an estrogen receptor antagonist demonstrated that the effect of the upregulated expression is connected with the estrogen receptor. In conclusion, ICA can regulate bone formation by promoting cell proliferation and differentiation and upregulating the OPG/RANKL expression ratio by the ER in hFOB1.19 human osteoblast cells.

Jet-spray apparatus for drying licorice root extract

1969 ◽  
Vol 3 (11) ◽  
pp. 665-667
Author(s):  
V. V. Davituliani ◽  
V. I. Ganuchukov
Keyword(s):  
Licorice Root ◽  
Root Extract ◽  
Licorice Root Extract

scholarly journals Glycyrrhizin effectively neutralizes SARS-CoV-2 in vitro by inhibiting the viral main protease

2020 ◽  
Author(s):  
L. van de Sand ◽  
M. Bormann ◽  
M. Alt ◽  
L. Schipper ◽  
C.S. Heilingloh ◽  
...  
Keyword(s):  
Licorice Root ◽  
Viral Diseases ◽  
Root Extract ◽  
Traditional Herbal Medicine ◽  
Complementary Treatment ◽  
Antiviral Compound ◽  
Viral Neutralization ◽  
Main Protease ◽  
Licorice Root Extract

AbstractThe newly emerged coronavirus, which was designated as severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the causative agent of the COVID-19 disease. High effective and well-tolerated medication for hospitalized and non-hospitalized patients is urgently needed. Traditional herbal medicine substances were discussed as promising candidates for the complementary treatment of viral diseases and recently suggested for the treatment of COVID-19. In the present study, we investigated aqueous licorice root extract for its neutralizing activity against SARS-CoV-2 in vitro, identified the active compound glycyrrhizin and uncovered the respective mechanism of viral neutralization. We demonstrated that glycyrrhizin, the primary active ingredient of the licorice root, potently neutralizes SARS-CoV-2 by inhibiting the viral main protease. Our experiments highlight glycyrrhizin as a potential antiviral compound that should be further investigated for the treatment of COVID-19.

Identification and quantification of isoflavonoid and triterpenoid compliance markers in a licorice-root extract powder

1993 ◽  
Vol 41 (1) ◽  
pp. 42-47 ◽  
Author(s):  
David S. Weinberg ◽  
M. Lisa. Mainer ◽  
Mark D. Richardson ◽  
Frederick G. Haibach
Keyword(s):  
Licorice Root ◽  
Root Extract ◽  
Licorice Root Extract ◽  
Identification And Quantification

scholarly journals AMELIORATION OF NECROSIS FORMATION IN GINGIVA BY LICORICE ROOT EXTRACT

2018 ◽  
Vol 11 (10) ◽  
pp. 137
Author(s):  
Shivanni Ss ◽  
Vishnu Priya V ◽  
Gayathri R
Keyword(s):  
Licorice Root ◽  
Tnf Alpha ◽  
Root Extract ◽  
Reactive Protein ◽  
Commercial Source ◽  
Bacterial Plaque ◽  
Reversible Condition ◽  
Hepatocyte Growth ◽  
Necrosis Factor ◽  
Licorice Root Extract

Objective: Gingivitis is a reversible condition associated with a bacterial plaque that resolves in about 1 week after the reinstitution of oral hygiene procedures. If left untreated, may ultimately progress to periodontitis in a subset of individuals. The objective of the study was to evaluate the amelioration of necrosis formation in gingiva by Licorice root extract. The licorice compounds, licoricidin, and licorisoflavan A were the most effective antibacterial substances.Methods: Licorice root extract was prepared by macerating 50 g of dry powder with 100 ml of 70% (w/v) ethyl alcohol for a week. Human gingival fibroblasts were obtained from a commercial source and were maintained in serum-free fibroblast medium as recommended by the manufacturer. The cells were treated with licorice root extract (100 μg/ml) and incubated for 48 h. Tumor necrosis factor (TNF) and C-reactive protein (CRP) were estimated.Result: TNF-alpha levels were raised, and agglutination of CRP was observed in hepatocyte growth factor positive samples whereas after the treatment with licorice root extract the TNF-alpha levels were declined and no agglutination was seen.Conclusion: TNF and CRP levels are raised during necrosis formation. These levels are decreased when treated with licorice root extract. Thus, licorice root extract causes amelioration of necrosis formation in the gingiva.

Identification and quantification of anticarcinogens in garlic extract and licorice root extract powder

1992 ◽  
Vol 15 (10) ◽  
pp. 641-654 ◽  
Author(s):  
David S. Weinberg ◽  
M. Lisa Manier ◽  
Mark D. Richardson ◽  
Frederick G. Haibach ◽  
Tina S. Rogers
Keyword(s):  
Garlic Extract ◽  
Licorice Root ◽  
Root Extract ◽  
Licorice Root Extract ◽  
Identification And Quantification
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