Detection of P16 and APC gene methylation in peripheral blood with liver cancer patients

Liver cancer is the fifth most common cancer in Indonesia. This research is to find out the prevalence and characteristics of liver cancer patients in Immanuel Hospital Bandung within the January 2013 until December 2014 period based on age, gender, clinical symptoms and predilections. This study was a descriptive verificative research with data retrieval of patients medical records that have been diagnosed with liver cancer that were hospitalized in Immanuel Hospital Bandung within January 2013 until December 2014 period. The study showed that the liver cancer patient prevalence in Immanuel Hospital Bandung within the period of January 2013 until December 2014 was 46 people. Characteristics of liver cancer patients in Immanuel Hospital Bandung within January 2013 until December 2014 period showed that the most liver cancer patients were male, compare with female with ratio 4:1, the most common age group of 56-65 years old, the most common clinical symptoms were abdominal pain with or without reffered pain to the right scapular bone and the most common predilection was right lobe of the liver. Key words: liver cancer, patients' characteristics, prevalence

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Real-Time PCR Analysis of Peripheral Blood: A Non-Invasive Technology that can be Used to Assess Tumor Burden in Breast Cancer Patients

10.21236/ada412843 ◽

2002 ◽

Author(s):

Michael Mitas

Keyword(s):

Breast Cancer ◽

Real Time ◽

Cancer Patients ◽

Real Time Pcr ◽

Peripheral Blood ◽

Tumor Burden ◽

Pcr Analysis ◽

Breast Cancer Patients ◽

Non Invasive

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Real-Time PCR Analysis of Peripheral Blood: A Non-Invasive Technology That Can Be Used to Assess Tumor Burden in Breast Cancer Patients

10.21236/ada424096 ◽

2003 ◽

Author(s):

Michael Mitas

Keyword(s):

Breast Cancer ◽

Real Time ◽

Cancer Patients ◽

Real Time Pcr ◽

Peripheral Blood ◽

Tumor Burden ◽

Pcr Analysis ◽

Breast Cancer Patients ◽

Non Invasive

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CK-19 Expression by RT-PCR in the Peripheral Blood of Breast Cancer Patients Correlates with Response to Chemotherapy

Breast Cancer Research and Treatment ◽

10.1023/a:1010621901102 ◽

2001 ◽

Vol 66 (3) ◽

pp. 249-254 ◽

Cited By ~ 14

Author(s):

Ana Rita Manhani ◽

Reinaldo Manhani ◽

Heloisa P. Soares ◽

Israel Bendit ◽

Fabiana Lopes ◽

...

Keyword(s):

Breast Cancer ◽

Cancer Patients ◽

Peripheral Blood ◽

Breast Cancer Patients ◽

Rt Pcr ◽

Response To Chemotherapy

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α-Particle-induced DNA damage tracks in peripheral blood mononuclear cells of [223Ra]RaCl2-treated prostate cancer patients

European Journal of Nuclear Medicine and Molecular Imaging ◽

10.1007/s00259-020-05170-6 ◽

2021 ◽

Author(s):

S. Schumann ◽

U. Eberlein ◽

C. Lapa ◽

J. Müller ◽

S. Serfling ◽

...

Keyword(s):

Prostate Cancer ◽

Dna Damage ◽

Cancer Patients ◽

Peripheral Blood Mononuclear Cells ◽

Peripheral Blood ◽

Mononuclear Cells ◽

Absorbed Dose ◽

Peripheral Blood Mononuclear ◽

Absorbed Doses ◽

Blood Mononuclear Cells

Abstract Purpose One therapy option for prostate cancer patients with bone metastases is the use of [223Ra]RaCl2. The α-emitter 223Ra creates DNA damage tracks along α-particle trajectories (α-tracks) in exposed cells that can be revealed by immunofluorescent staining of γ-H2AX+53BP1 DNA double-strand break markers. We investigated the time- and absorbed dose-dependency of the number of α-tracks in peripheral blood mononuclear cells (PBMCs) of patients undergoing their first therapy with [223Ra]RaCl2. Methods Multiple blood samples from nine prostate cancer patients were collected before and after administration of [223Ra]RaCl2, up to 4 weeks after treatment. γ-H2AX- and 53BP1-positive α-tracks were microscopically quantified in isolated and immuno-stained PBMCs. Results The absorbed doses to the blood were less than 6 mGy up to 4 h after administration and maximally 16 mGy in total. Up to 4 h after administration, the α-track frequency was significantly increased relative to baseline and correlated with the absorbed dose to the blood in the dose range < 3 mGy. In most of the late samples (24 h – 4 weeks after administration), the α-track frequency remained elevated. Conclusion The γ-H2AX+53BP1 assay is a potent method for detection of α-particle-induced DNA damages during treatment with or after accidental incorporation of radionuclides even at low absorbed doses. It may serve as a biomarker discriminating α- from β-emitters based on damage geometry.

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