scholarly journals Selection of the optimal extractant for the extraction of phenolic compounds from Laurus nobilis L. leaves

2021 ◽  
Vol 19 (4(76)) ◽  
pp. 60-64
Author(s):  
Irina Yu. Posokhova ◽  
Kateryna S. Skrebtsova ◽  
Olga P. Khvorost ◽  
Yuliia A. Fedchenkova
Keyword(s):  
Phenolic Compounds ◽  
Quantitative Determination ◽  
Spectrophotometric Method ◽  
Rosmarinic Acid ◽  
Hydroxycinnamic Acids ◽  
Laurus Nobilis ◽  
Quantitative Content ◽  
First Time ◽  
Selection Of

Aim. To select the optimal extractant for the extraction of a number of groups of phenolic compounds from Lаurus nоbilis L. leaves based on the determination of the quantitative content of these groups in the extracts obtained. Materials and methods. Lаurus nоbilis L. leaves were harvested in November 2020 from artificially cultivated specimens aged 5 – 7 years. The quantitative determination of polyphenols was performed using the spectrophotometric method at a wavelength of 760 nm in accordance with the requirements of the Supplement of the State Pharmacopoeia of Ukraine (SPhU) 1.2 (2.8.14). The quantitative content of this group of compounds was calculated with reference to pyrogalol and dried substance. The quantitative determination of the amount of hydroxycinnamic acids was performed according to the SPhU 2.2 monograph “Orthosyphon stamen (kidney tea) leavesN” by the spectrophotometric method at a wavelength of 505 nm and calculated with reference to rosmarinic acid. The quantitative determination of the amount of flavonoids was performed according to the SPhU 2.0 monograph “Hawthorn leaves and flowers” by the spectrophotometric method at a wavelength of 410 nm and calculated with reference to hyperoside.Results and discussion. The analysis of the results for the quantitative determination of polyphenols, the amount of hydroxycinnamic acids and the amount of flavonoids in extracts from Lаurus nоbilis L. leaves obtained using water, water-ethanol mixtures and 96 % ethanol showed that 70 % ethanol was optimal for extracting compounds of these groups. The quantitative content of polyphenols (calculated with reference to pyrogalol) was not less than 21 %, the amount of hydroxycinnamic acids (calculated with reference to rosmarinic acid) – not less than 3 %, the amount of flavonoids (calculated with reference to hyperoside) – not less than 5 %. Conclusions. For the first time, the quantitative content of a number of groups of phenolic compounds (polyphenols, the total amount of hydroxycinnamic acids, and the total amount of flavonoids) in Lаurus nоbilis L. leaves extracts obtained using various extractants has been determined. On this basis, the optimal extractant – 70 % ethanol for the extraction of phenolic compounds has been selected. The results obtained will be used when developing the technology for obtaining substances from Lаurus nоbilis L. leaves.

scholarly journals The study of the qualitative composition and the quantitative content of phenolic compounds in dietary supplements with lingonberry

2021 ◽  
Vol 19 (4(76)) ◽  
pp. 40-46
Author(s):  
Oleksandr Yu. Maslov ◽  
Mykola A. Komisarenko ◽  
Serhii V. Kolisnyk ◽  
Olga V. Antonenko ◽  
Olena V. Kolisnyk ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Dietary Supplements ◽  
Total Content ◽  
Hydroxycinnamic Acids ◽  
Biologically Active ◽  
Qualitative Composition ◽  
Relevant Today ◽  
Quantitative Content ◽  
Layer Chromatography

Aim. Today, there are a lot of dietary supplements with lingonberry at the pharmaceutical market of Ukraine; therefore, the analysis and quality control of these products are relevant. In this connection, the aim of the research was to study the qualitative composition and determine the quantitative content of phenolic compounds in dietary supplements with lingonberry.Materials and methods. Such dietary supplements as “Extract of lingonberry” (MEDAGROPROM), “Lingonberry” (Danikafarm), “Lingonberry nano” (LSS SYSTEM) were chosen for the study. The qualitative analysis was performed by thin layer chromatography (TLC), spectrophotometry was used for the quantitative determination.Results and discussion. Hydroquinone derivatives, flavonoids and hydroxycinnamic acids were found in the dietary supplements analyzed. The total content of phenolic compounds was 8.70, 0.26, 0.30 %, flavonoids – 6.37, 0.15, 0.12 %, hydroxycinnamic acids – 0.94, 0.06, 0.13 %, and hydroquinone derivatives – 1.01, 0.04, 0.03 % in such dietary supplements as “Extract of lingonberry” (MEDAGROPROM), “Lingonberry” (Danikafarm), “Lingonberry nano” (LSS SYSTEM), respectively. Conclusions. The qualitative and quantitative analysis of the dietary supplements with lingonberry analyzed has been performed. “Extract of lingonberry” (MEDAGROPROM) dietary supplement meets the requirements of the State of Pharmacopoeia of Ukraine 2.0, whereas “Lingonberry” (Danikafarm) and “Lingonberry nano” (LSS SYSTEM) do not. Based on the results of the study it can be concluded that the problem of compliance of dietary supplements is relevant today and requires the introduction of regulatory documentation for the detection and determination of biologically active substances in dietary supplements.

scholarly journals PHENOLIC COMPOUNDS SANGUISORBA OFFICINALIS (ROSACEAE) GROWING IN EASTERN SIBERIA

2021 ◽  
pp. 139-150
Author(s):  
Tat'yana Mikhaylovna Shishmareva ◽  
Vyacheslav Mikhaylovich Shishmarev ◽  
Daniil Nikolayevich Olennikov
Keyword(s):  
Regression Analysis ◽  
Phenolic Compounds ◽  
Spectrophotometric Method ◽  
Mass Spectra ◽  
Eastern Siberia ◽  
Chromatographic Mobility ◽  
Quantitative Content ◽  
Sanguisorba Officinalis ◽  
First Time ◽  
Chromatographic Profiles

The present work aimed at the study of the phenolic compounds of Sanguisorba officinalis L. (Rosaceae) growing in Eastern Siberia. The quantitative content of flavonoids in S. officinalis extracts determined by the spectrophotometric method was 0.68–5.41% in herb and 0.20–0.51% in rhizomes and roots. Catechin content was 0.05–0.75% in herb and 0.08–9.86% in rhizomes and roots, and procyanidin content found with spectrophotometric Porter method was 0.02–1.45% in herb and 0.08–1.05% in rhizomes and roots. Regression analysis of the relationships “latitude / longitude / altitude – phenolic compounds content” showed the high values of the correlation coefficient for the catechin content in herb and roots of S. officinalis and the longitude of the place of growth, as well as altitude. The content of flavonoids in the herb and roots of S. officinalis was connected with the longitude and latitude of the collection place. The values of procyanidin content in S. officinalis organs and geographic indicators have fewer relationships. Using the HPLC-DAD-ESI-MS method, chromatographic profiles of the herb and rhizomes and roots of S. officinalis was investigated. The data of chromatographic mobility, UV and mass spectra allowed estimating 52 compounds, including 45 in herb and 31 in rhizomes and roots. The members of seven groups of phenolic compounds have been found in S. officinalis, including gallotannins, flavonol-O-glycosides, procyanidins, ellagotannins, hydroxycinnamates, catechins, and pyrones, of which 12 are previously described for the species and the presence of 40 components, has been shown for the first time in the species.

scholarly journals ДОСЛІДЖЕННЯ ФЕНОЛЬНИХ СПОЛУК РОЗХІДНИКА ЗВИЧАЙНОГО (GLECHOMA HEDERACEA L.)

2015 ◽  
Author(s):  
М. С. Гарник
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Phenolic Compounds ◽  
Spectrophotometric Method ◽  
High Performance ◽  
Hydroxycinnamic Acid ◽  
Hydroxycinnamic Acids ◽  
Sample Number ◽  
Quantitative Content ◽  
Glechoma Hederacea

<p><strong>THE INVESTIGATION OF COMPOUNDS PHENOL ORIGIN GROUND IVY (GLECHOMA HEDERACEA L.)</strong></p><p><strong>M. S. Garnyk</strong></p><p>NationalPyrogovMemorialMedicalUniversity, Vinnytsia</p><p><strong>Summary:</strong> compounds of phenol origin have been quantified in ground ivy herb, they are hydroxycinnamic acids (4.20-6.47 %), flavonoids (0.77-1.35 %), oxidized phenols (7.75-8.51 %). Flavonoid groups (rutin, hyperozide, apigenin, luteolin, isoquercitrin), coumarines (scopoletin, umbelliferone) hydroxycinnamic acids (rosmarinic, chlorogenic, caffeic, p-coumaric) have been identified by the method of HPLC and their content has been identified.</p><p><strong>Key words</strong>: ground ivy, compounds of phenol origin, spectrophotometry, permanganatometry, high performance liquid chromatography.</p><p><strong>Introduction.</strong> The ability to synthesize and accumulate huge amount of phenolic compounds is a characteristic feature of the representatives of the plant world. Natural phenols exhibit high biological activity. They take part in various physiological processes: respiration, photosynthesis, growth, development and reproduction of plants; some polyphenols protect plants from pathogens and fungal diseases.</p><p>Medicinal products based on phenolic compounds are widely used as anti-microbial, anti-inflammatory, hemostatic, choleretic, diuretic, hypotensive, tonic, astringent and purgative means. Most of the phenolic compounds of plant origin have low toxicity and do not exhibit side effects.</p><p>Previous studies found that grass of ground ivy contains a number of phenolic compounds namely: hydroxycinnamic acids, flavonoids, tannins. Therefore the purpose of the study was to determine the quantitative content of phenolic compounds by spectrophotometric method and individual substances by high performance liquid chromatography.</p><p><strong>Methods.</strong> The determination of phenolic compounds recalculated to gallic acid has been performed by spectrophotometric method at a wavelength of 270 nm, the amount of hydroxycinnamic acids recalculated to chlorogenic acid – at a wavelength of 327 nm, the amount of flavonoids recalculated to rutin – at a wavelength of 410 nm; total oxidized amount of phenols has been determined by permanganatometrical titration. To separate the total amount of phenolic compounds into individual components high performance liquid chromatography was used.</p><p><strong>Results and discussion.</strong> The content of phenolic compounds in grass of ground ivy was 3.15-3.41% in the sample number 1 (raw materials harvested in Ternopil region) and 2.98-3.29 % in the sample number 2 (materials harvested in Vinnytsia region), the total content of oxidized phenols is 8.34-8.51 % and 7.75-8.14 %, the amount of flavonoids – 0.87-1.35% and 0.77-1.04 %, the amount hydroxycinnamic acids – 4.20-6.47 % and 4.84-6.36 % respectively for the objects number 1 and number 2.</p><p>Individual quantitative content of phenolic compounds in the grass of ground ivy was identified by the method of HPLC. Flavonoids (rutin, hyperozide, apigenin, luteolin, isoquercitrin) were identified in the studied objects, as well as coumarins (skopoletyn, umbelliferone), hydroxycinnamic acid (rosmary, chlorogenic, coffeic, p-coumaric), and their quantitative content was determined.</p><p>Among flavonoid compounds of ground ivy the greatest number falls to rutin – 0.40 and 0.23 % respectively in objects number 1 and 2. As for hydroxycinnamic acid the highest content of rosmarinic acid is observed in the sample number 2 – 1.13 %. The content of chlorogenic acid was also significant and made 0.84 % (№ 1) and 0.81 % (№ 2) in studied objects.</p><p><strong>Conclusions.</strong> 1. Quantitative content of phenolic compounds: total amount of oxidative phenols, total amount of hydroxycinnamic acids, total amount of flavonoids were detected in the studied samples of ground ivy.</p><p>2. Quantitative content of flavonoids (rutin – 0.23-0.40 %, hyperozide – 0.17-0.34 %, luteolin – 0.05-0.1% isoquercіtrіn – 0.02-0.03 %, apigenin – 0.06 %), hydroxycinnamic acids (chlorogenic – 0.81-0.84 %, rosmarinic – 0.2-1.13%, coffeic – 0.06 %, p-coumaric – traces) and coumarins (skopoletyn – 0.11-0.12 %, umbelliferonе – 0.02 %) was identified and defined by the method of HPLC.</p>

scholarly journals Визначення кількісного вмісту суми фенольних сполукв артишоку суцвіттях, часнику листі та цибулинах

2018 ◽  
Author(s):  
A. I. Fedosov ◽  
V. S. Kyslychenko ◽  
O. M. Novosel
Keyword(s):  
Phenolic Compounds ◽  
Quantitative Determination ◽  
Gallic Acid ◽  
Plant Species ◽  
Medical Practice ◽  
Wide Distribution ◽  
Biologically Active ◽  
Active Compounds ◽  
Quantitative Content

Introduction. Phenolic compounds are the most abundant class of biologically active compounds of herbal origin. They possess antioxidant, anti-tumor, antimutagenic, antisclerotic, antibacterial, antiviral, sedative, anti-ulcer and anti-inflammatory activity. Wide distribution of phenolic compounds in the plant world and their usage in medical practice provide the importance of their quantitative determination in prospective plant species, such as artichoke and garlic.The purpose of current work was determination of quantitative content of phenolic compounds in artichoke inflorescences, garlic leaves and bulbs.Methods of research. The content of phenolic compounds was determined using various methods: permanganatometric, spectrophotometric calculated on gallic acid, spectrophotometric according to the SPU method.Results and discussion. The content of the sum of phenolic compounds, determined by permanganatometry, comprised 9,74% in artichoke inflorescences, 8,96% in garlic leaves and 4,85% – in garlic bulbs; determined spectrophotometrically calculated on gallic acid in artichoke inflorescences was 6,58%, in garlic leaves – 6,22%, in garlic bulbs – 2,87%; determined spectrophotometrically according to the SPU method in artichoke inflorescences comprised 4,43%, in garlic leaves – 3,91%, and in garlic bulbs – 2,11%.Conclusions.The quantitative content of phenolic compounds in artichoke, garlic leaves and bulbs was determined using various methods: permanganatometric, spectrophotometric calculated on gallic acid, spectrophotometric according to the SPU method.As a result of the experiment, it was found that phenolic compounds accumulate in the artichoke inflorescences and garlic leaves, and in garlic bulbs their content is lower.The results obtained allow the recommending artichoke inflorescences, garlic leaves and bulbs as the herbal sources of phenolic compounds.

scholarly journals Polyphenolic compounds and essential oil analysis of selected species of the genus Thymus / Analýza fenolových zložiek a analýza silice vybraných druhov rodu Thymus

2015 ◽  
Vol 62 (s9) ◽  
pp. 12-17
Author(s):  
M. Kameníková ◽  
S. Fialová ◽  
A. Ťažký ◽  
I. Čičová
Keyword(s):  
Phenolic Compounds ◽  
Essential Oil ◽  
Spectrophotometric Method ◽  
Total Phenolics ◽  
Chemical Constituents ◽  
Aluminium Chloride ◽  
Total Phenolic ◽  
Oil Analysis ◽  
European Pharmacopoeia

Abstract The content of phenolic compounds (total phenolic compounds, tannins, flavonoids and anthocyanidins) of three species of thyme (Thymus pulegioides, Thymus pannonicus, Thymus praecox) of different origin (Bohemian-Moravian highlands, Křivoklat and Považsky Inovec) was determined using spectrophotometric methods of European Pharmacopoeia 8th edition. Furthermore, the determination of the content of essential oil and analysis of its constituents was realised. The amount of total phenolics was determined by a spectrophotometric method using the Folin-Ciocalteu reagent. Their content ranged from 3.87 to 8.86%. The content of tannins was established on a preliminary determination of the total phenolics, followed by adsorption of tannins on hide powder. The amount of tannins in our samples varied from 1.96 to 5.65%. The content of total flavonoids was determined by a spectrophotometric method using aluminium chloride. Quantitative analysis of flavonoids has shown that content in our samples ranged from 0.59 to 1.52% expressed as luteolin-7-O-glucoside (λ = 392 nm) and from 0.41 to 1.12% expressed as rutin (λ = 420 nm). Anthocyanins represent a small amount of total polyphenol content in Thymus species. In our samples, the content of anthocyanins expressed as cyanidin-3-O-glucoside varied from 0.02 to 0.1%. The determination of essential oil was realised by method of European Pharmacopoeia 8th edition. Its contents in our samples ranged from 0.2 to 0.75%. Gas chromatography-mass spectrometry was used for essential oil analysis. According to the presence of main monoterpene in Thymus essential oil, we can distinguish three chemotypes: thymol, carvacrol and linalool. Due to variations of chemical constituents of thyme essential oil, it was possible to observe a relationship between occurrence of certain chemotype and origin of sample. Differences in content of flavonoids have not shown a significant relationship to locality of origin.

scholarly journals QUANTITATIVE DETERMINATION OF PHENOL CARBOXYLIC ACIDS IN POMEGRANATE FRUIT PULP BY THE HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY WITH ULTRAVIOLET DETECTION

2019 ◽  
Vol 12 (1) ◽  
pp. 548
Author(s):  
Ruben Ashotovich Pogosyan ◽  
Olga Vladimirovna Nesterova ◽  
Dmitry Olegovich Bokov ◽  
Irina Alexandrovna Samylina
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Phenolic Compounds ◽  
Quantitative Determination ◽  
Gallic Acid ◽  
Carboxylic Acids ◽  
High Performance ◽  
Fruit Pulp ◽  
Pomegranate Fruit

Objective: Pomegranate (Punica granatum L.) is a broadly used plant possessing a wide range of medicinal properties. In this research, we have mainly focused on the investigation of phenolic compounds of pomegranate fruit pulp (PFP).Methods: Fresh fruits of “Çəhrayı Gülöyşə,” “Kizil-anor,” and pomegranate varietal mixture were used as samples. High-performance liquid chromatography-ultraviolet (HPLC-UV) analysis of phenol carboxylic acids was performed with metal column Kromasil® C18 (4.6×250 mm, particle size 5 μm) and the acetonitrile-water-concentrated acid phosphoric system (400:600:5) under isocratic elution conditions (flow rate of 0.5 ml/min). Detection was carried out using a UV detector “GILSTON” UV/Visible model 151 at a wavelength of 280 nm.Results and Discussion: As a result of our research, we proposed chromatographic conditions for the separation of phenolic compounds, the conditions for sample preparation of PFP. Procedure for determination of phenolic carboxylic acids total content in terms of gallic acid by HPLC-UV method was developed. According to the obtained data, the content of phenolic carboxylic acids should be at least 0.7%.Conclusion: Procedure for the quantitative determination of gallic acid using the HPLC-UV method was developed. This method which can be used in the standardization of new medicinal plant raw materials - PFP, as well as extract preparations based on it in the future.

scholarly journals Determination of hydroxycinnamic acids in the herb of stachys sieboldii miq

2016 ◽  
Author(s):  
S. M. Marchyshyn ◽  
L. V. Husak ◽  
T. S. Berdey
Keyword(s):  
Hydroxycinnamic Acids ◽  
Qualitative Composition ◽  
Quantitative Content ◽  
Stachys Sieboldii

In the article the results of study of qualitative composition and quantitative content of hydroxycinnamic acids in the herb of Stachys sieboldii Miq. are presented. By the method  of HPLC it was established the presence of chlorogenic, rosemarinic, caffeic and p-coumaric acids. Quantitative content of hydroxycinnamic acids was determined by the method of spectrophotometry.

scholarly journals QUANTITATIVE DETERMINATION OF PHENOL CARBOXYLIC ACIDS IN POMEGRANATE FRUIT PULP BY THE HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY WITH ULTRAVIOLET DETECTION

2019 ◽  
Vol 12 (1) ◽  
pp. 548
Author(s):  
Ruben Ashotovich Pogosyan ◽  
Olga Vladimirovna Nesterova ◽  
Dmitry Olegovich Bokov ◽  
Irina Alexandrovna Samylina
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Phenolic Compounds ◽  
Quantitative Determination ◽  
Gallic Acid ◽  
Carboxylic Acids ◽  
High Performance ◽  
Fruit Pulp ◽  
Pomegranate Fruit

Objective: Pomegranate (Punica granatum L.) is a broadly used plant possessing a wide range of medicinal properties. In this research, we have mainly focused on the investigation of phenolic compounds of pomegranate fruit pulp (PFP).Methods: Fresh fruits of “Çəhrayı Gülöyşə,” “Kizil-anor,” and pomegranate varietal mixture were used as samples. High-performance liquid chromatography-ultraviolet (HPLC-UV) analysis of phenol carboxylic acids was performed with metal column Kromasil® C18 (4.6×250 mm, particle size 5 μm) and the acetonitrile-water-concentrated acid phosphoric system (400:600:5) under isocratic elution conditions (flow rate of 0.5 ml/min). Detection was carried out using a UV detector “GILSTON” UV/Visible model 151 at a wavelength of 280 nm.Results and Discussion: As a result of our research, we proposed chromatographic conditions for the separation of phenolic compounds, the conditions for sample preparation of PFP. Procedure for determination of phenolic carboxylic acids total content in terms of gallic acid by HPLC-UV method was developed. According to the obtained data, the content of phenolic carboxylic acids should be at least 0.7%.Conclusion: Procedure for the quantitative determination of gallic acid using the HPLC-UV method was developed. This method which can be used in the standardization of new medicinal plant raw materials - PFP, as well as extract preparations based on it in the future.

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