scholarly journals Визначення кількісного вмісту суми фенольних сполукв артишоку суцвіттях, часнику листі та цибулинах

2018 ◽  
Author(s):  
A. I. Fedosov ◽  
V. S. Kyslychenko ◽  
O. M. Novosel
Keyword(s):  
Phenolic Compounds ◽  
Quantitative Determination ◽  
Gallic Acid ◽  
Plant Species ◽  
Medical Practice ◽  
Wide Distribution ◽  
Biologically Active ◽  
Active Compounds ◽  
Quantitative Content

Introduction. Phenolic compounds are the most abundant class of biologically active compounds of herbal origin. They possess antioxidant, anti-tumor, antimutagenic, antisclerotic, antibacterial, antiviral, sedative, anti-ulcer and anti-inflammatory activity. Wide distribution of phenolic compounds in the plant world and their usage in medical practice provide the importance of their quantitative determination in prospective plant species, such as artichoke and garlic.The purpose of current work was determination of quantitative content of phenolic compounds in artichoke inflorescences, garlic leaves and bulbs.Methods of research. The content of phenolic compounds was determined using various methods: permanganatometric, spectrophotometric calculated on gallic acid, spectrophotometric according to the SPU method.Results and discussion. The content of the sum of phenolic compounds, determined by permanganatometry, comprised 9,74% in artichoke inflorescences, 8,96% in garlic leaves and 4,85% – in garlic bulbs; determined spectrophotometrically calculated on gallic acid in artichoke inflorescences was 6,58%, in garlic leaves – 6,22%, in garlic bulbs – 2,87%; determined spectrophotometrically according to the SPU method in artichoke inflorescences comprised 4,43%, in garlic leaves – 3,91%, and in garlic bulbs – 2,11%.Conclusions.The quantitative content of phenolic compounds in artichoke, garlic leaves and bulbs was determined using various methods: permanganatometric, spectrophotometric calculated on gallic acid, spectrophotometric according to the SPU method.As a result of the experiment, it was found that phenolic compounds accumulate in the artichoke inflorescences and garlic leaves, and in garlic bulbs their content is lower.The results obtained allow the recommending artichoke inflorescences, garlic leaves and bulbs as the herbal sources of phenolic compounds.

scholarly journals QUANTITATIVE DETERMINATION OF PHENOL CARBOXYLIC ACIDS IN POMEGRANATE FRUIT PULP BY THE HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY WITH ULTRAVIOLET DETECTION

2019 ◽  
Vol 12 (1) ◽  
pp. 548
Author(s):  
Ruben Ashotovich Pogosyan ◽  
Olga Vladimirovna Nesterova ◽  
Dmitry Olegovich Bokov ◽  
Irina Alexandrovna Samylina
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Phenolic Compounds ◽  
Quantitative Determination ◽  
Gallic Acid ◽  
Carboxylic Acids ◽  
High Performance ◽  
Fruit Pulp ◽  
Pomegranate Fruit

Objective: Pomegranate (Punica granatum L.) is a broadly used plant possessing a wide range of medicinal properties. In this research, we have mainly focused on the investigation of phenolic compounds of pomegranate fruit pulp (PFP).Methods: Fresh fruits of “Çəhrayı Gülöyşə,” “Kizil-anor,” and pomegranate varietal mixture were used as samples. High-performance liquid chromatography-ultraviolet (HPLC-UV) analysis of phenol carboxylic acids was performed with metal column Kromasil® C18 (4.6×250 mm, particle size 5 μm) and the acetonitrile-water-concentrated acid phosphoric system (400:600:5) under isocratic elution conditions (flow rate of 0.5 ml/min). Detection was carried out using a UV detector “GILSTON” UV/Visible model 151 at a wavelength of 280 nm.Results and Discussion: As a result of our research, we proposed chromatographic conditions for the separation of phenolic compounds, the conditions for sample preparation of PFP. Procedure for determination of phenolic carboxylic acids total content in terms of gallic acid by HPLC-UV method was developed. According to the obtained data, the content of phenolic carboxylic acids should be at least 0.7%.Conclusion: Procedure for the quantitative determination of gallic acid using the HPLC-UV method was developed. This method which can be used in the standardization of new medicinal plant raw materials - PFP, as well as extract preparations based on it in the future.

scholarly journals QUANTITATIVE DETERMINATION OF PHENOL CARBOXYLIC ACIDS IN POMEGRANATE FRUIT PULP BY THE HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY WITH ULTRAVIOLET DETECTION

2019 ◽  
Vol 12 (1) ◽  
pp. 548
Author(s):  
Ruben Ashotovich Pogosyan ◽  
Olga Vladimirovna Nesterova ◽  
Dmitry Olegovich Bokov ◽  
Irina Alexandrovna Samylina
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Phenolic Compounds ◽  
Quantitative Determination ◽  
Gallic Acid ◽  
Carboxylic Acids ◽  
High Performance ◽  
Fruit Pulp ◽  
Pomegranate Fruit

Objective: Pomegranate (Punica granatum L.) is a broadly used plant possessing a wide range of medicinal properties. In this research, we have mainly focused on the investigation of phenolic compounds of pomegranate fruit pulp (PFP).Methods: Fresh fruits of “Çəhrayı Gülöyşə,” “Kizil-anor,” and pomegranate varietal mixture were used as samples. High-performance liquid chromatography-ultraviolet (HPLC-UV) analysis of phenol carboxylic acids was performed with metal column Kromasil® C18 (4.6×250 mm, particle size 5 μm) and the acetonitrile-water-concentrated acid phosphoric system (400:600:5) under isocratic elution conditions (flow rate of 0.5 ml/min). Detection was carried out using a UV detector “GILSTON” UV/Visible model 151 at a wavelength of 280 nm.Results and Discussion: As a result of our research, we proposed chromatographic conditions for the separation of phenolic compounds, the conditions for sample preparation of PFP. Procedure for determination of phenolic carboxylic acids total content in terms of gallic acid by HPLC-UV method was developed. According to the obtained data, the content of phenolic carboxylic acids should be at least 0.7%.Conclusion: Procedure for the quantitative determination of gallic acid using the HPLC-UV method was developed. This method which can be used in the standardization of new medicinal plant raw materials - PFP, as well as extract preparations based on it in the future.

scholarly journals The study of the qualitative composition and the quantitative content of phenolic compounds in dietary supplements with lingonberry

2021 ◽  
Vol 19 (4(76)) ◽  
pp. 40-46
Author(s):  
Oleksandr Yu. Maslov ◽  
Mykola A. Komisarenko ◽  
Serhii V. Kolisnyk ◽  
Olga V. Antonenko ◽  
Olena V. Kolisnyk ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Dietary Supplements ◽  
Total Content ◽  
Hydroxycinnamic Acids ◽  
Biologically Active ◽  
Qualitative Composition ◽  
Relevant Today ◽  
Quantitative Content ◽  
Layer Chromatography

Aim. Today, there are a lot of dietary supplements with lingonberry at the pharmaceutical market of Ukraine; therefore, the analysis and quality control of these products are relevant. In this connection, the aim of the research was to study the qualitative composition and determine the quantitative content of phenolic compounds in dietary supplements with lingonberry.Materials and methods. Such dietary supplements as “Extract of lingonberry” (MEDAGROPROM), “Lingonberry” (Danikafarm), “Lingonberry nano” (LSS SYSTEM) were chosen for the study. The qualitative analysis was performed by thin layer chromatography (TLC), spectrophotometry was used for the quantitative determination.Results and discussion. Hydroquinone derivatives, flavonoids and hydroxycinnamic acids were found in the dietary supplements analyzed. The total content of phenolic compounds was 8.70, 0.26, 0.30 %, flavonoids – 6.37, 0.15, 0.12 %, hydroxycinnamic acids – 0.94, 0.06, 0.13 %, and hydroquinone derivatives – 1.01, 0.04, 0.03 % in such dietary supplements as “Extract of lingonberry” (MEDAGROPROM), “Lingonberry” (Danikafarm), “Lingonberry nano” (LSS SYSTEM), respectively. Conclusions. The qualitative and quantitative analysis of the dietary supplements with lingonberry analyzed has been performed. “Extract of lingonberry” (MEDAGROPROM) dietary supplement meets the requirements of the State of Pharmacopoeia of Ukraine 2.0, whereas “Lingonberry” (Danikafarm) and “Lingonberry nano” (LSS SYSTEM) do not. Based on the results of the study it can be concluded that the problem of compliance of dietary supplements is relevant today and requires the introduction of regulatory documentation for the detection and determination of biologically active substances in dietary supplements.

scholarly journals Use of Capillary Electrophoresis Method in Determination of Quantitative Content of Mangiferin in Grass of Species of Genus Hedysarum (Hedysarum Caucasicum M.Bieb., Hedysarum Grandiflorum Pall., Hedysarum Daghestanicum Rupr. ex Boiss) of flora of the North Caucasus

2021 ◽  
Vol 10 (1) ◽  
pp. 90-96
Author(s):  
D. R. Imachueva ◽  
F. K. Serebryanaya
Keyword(s):  
Capillary Electrophoresis ◽  
Quantitative Determination ◽  
Botanical Garden ◽  
Biologically Active ◽  
North Caucasus ◽  
The North ◽  
Capillary Electrophoresis Method ◽  
Quantitative Content ◽  
Electrophoresis Method

Introduction. The species of the genus Hedysarum L. of the Fabaceae family are of significant interest in connection with the accumulation of a group of biologically active substances with antibacterial and antiviral activity against DNA-containing viruses.Aim. The purpose of the work is to develop a method for the quantitative determination of mangiferin in the aboveground part of species of the genus Hedysarum L., Hedysarum caucasicum M.Bieb., Hedysarum grandiflorum Pall., Hedysarum daghestanicum Rupr. ex. Boiss. collected in the North Caucasus by zonal capillary electrophoresis.Materials and methods. The objects of the study were species of the genus Hedysarum L., namely Hedysarum caucasicum M.Bieb., Hedysarum grandiflorum Pall., Hedysarum daghestanicum Rupr. ex. Boiss. collected in the North Caucasus. Registration of electronic spectra was carried out on capillary electrophoresis "KAPEL'®-105m" (OJSC "Lumex-marketing", Russia) with quartz capillary Leff./Ltol. = 50/60 cm, ID = 75 μm. As a standard sample, mangiferin (substance-powder, mangiferin content ≥98 %, Sigma-Aldrich, Lot SLBP4044V) was used.Results and discussion. As a result of comprehensive pharmacognostic studies of species of the kopecki genus growing in the North Caucasus under natural conditions, as well as under the conditions of introduction on the territory of the Botanical Garden of the Pyatigorsk Medical and Pharmaceutical Institute and the Mountain Botanical Garden of the Dagestan Federal Research Center of the Russian Academy of Sciences, we developed a method for the isolation and quantitative determination of mangiferin by capillary electrophoresis.Conclusion. Studies show that the use of the zonal capillary electrophoresis method in the analysis of xanthones, including the determination of the quantitative content of mangiferin, is promising. The largest content of mangiferin (0.25 %) is distinguished by Hedysarum caucasicum M.Bieb., which confirms the assumption based on molecular genetic studies, since it is this species that belongs to the Obscura section, as well as the alpine penny used to produce mangiferin. Therefore, it is possible to provide this technique for analysis of mangiferin xanthone glycoside along with spectrophotometry and high performance liquid chromatography.

scholarly journals Selection of the optimal extractant for the extraction of phenolic compounds from Laurus nobilis L. leaves

2021 ◽  
Vol 19 (4(76)) ◽  
pp. 60-64
Author(s):  
Irina Yu. Posokhova ◽  
Kateryna S. Skrebtsova ◽  
Olga P. Khvorost ◽  
Yuliia A. Fedchenkova
Keyword(s):  
Phenolic Compounds ◽  
Quantitative Determination ◽  
Spectrophotometric Method ◽  
Rosmarinic Acid ◽  
Hydroxycinnamic Acids ◽  
Laurus Nobilis ◽  
Quantitative Content ◽  
First Time ◽  
Selection Of

Aim. To select the optimal extractant for the extraction of a number of groups of phenolic compounds from Lаurus nоbilis L. leaves based on the determination of the quantitative content of these groups in the extracts obtained. Materials and methods. Lаurus nоbilis L. leaves were harvested in November 2020 from artificially cultivated specimens aged 5 – 7 years. The quantitative determination of polyphenols was performed using the spectrophotometric method at a wavelength of 760 nm in accordance with the requirements of the Supplement of the State Pharmacopoeia of Ukraine (SPhU) 1.2 (2.8.14). The quantitative content of this group of compounds was calculated with reference to pyrogalol and dried substance. The quantitative determination of the amount of hydroxycinnamic acids was performed according to the SPhU 2.2 monograph “Orthosyphon stamen (kidney tea) leavesN” by the spectrophotometric method at a wavelength of 505 nm and calculated with reference to rosmarinic acid. The quantitative determination of the amount of flavonoids was performed according to the SPhU 2.0 monograph “Hawthorn leaves and flowers” by the spectrophotometric method at a wavelength of 410 nm and calculated with reference to hyperoside.Results and discussion. The analysis of the results for the quantitative determination of polyphenols, the amount of hydroxycinnamic acids and the amount of flavonoids in extracts from Lаurus nоbilis L. leaves obtained using water, water-ethanol mixtures and 96 % ethanol showed that 70 % ethanol was optimal for extracting compounds of these groups. The quantitative content of polyphenols (calculated with reference to pyrogalol) was not less than 21 %, the amount of hydroxycinnamic acids (calculated with reference to rosmarinic acid) – not less than 3 %, the amount of flavonoids (calculated with reference to hyperoside) – not less than 5 %. Conclusions. For the first time, the quantitative content of a number of groups of phenolic compounds (polyphenols, the total amount of hydroxycinnamic acids, and the total amount of flavonoids) in Lаurus nоbilis L. leaves extracts obtained using various extractants has been determined. On this basis, the optimal extractant – 70 % ethanol for the extraction of phenolic compounds has been selected. The results obtained will be used when developing the technology for obtaining substances from Lаurus nоbilis L. leaves.

scholarly journals QUANTITATIVE DETERMINATION OF THE SUM OF PHENOLIC COMPOUNDS IN FRUIT OF RHUS TYPHINA(L.)

2019 ◽  
pp. 225-232
Author(s):  
Виктория (Viktoria) Нодарьевна (Nodar'evna) Леонова (Leonova) ◽  
Иван (Ivan) Викторович (Viktorovich) Попов (Popov) ◽  
Ольга (Ol'ga) Ивановна (Ivanovna) Попова (Popova) ◽  
Владимир (Vladimir) Павлович (Pavlovich) Зайцев (Zaitsev)
Keyword(s):  
Phenolic Compounds ◽  
Quantitative Determination ◽  
Gallic Acid ◽  
Calibration Graph ◽  
Water Extraction ◽  
Complexation Reaction ◽  
Alcohol Water ◽  
Relative Standard ◽  
Rhus Typhina

The genus Rhus of the Anacardiaceae family includes about 120–150 species of small trees, bushes, and lianas. The chemical composition of the sumac fluffy Rhus typhina (L.) is almost unknown. Previously, some phenolic compounds (rutin, quercetin, hyperoside, tannin, gallic acid) were identified in the fruits of the plant. To quantify the amount of phenolic compounds in the raw materials studied, a method based on the complexation reaction of phenolic compounds with a phosphomolybdic-tungsten reagent (Folin-Denis reagent) in an alkaline medium was chosen. The optimal conditions for the complexation reaction are selected: the ratio of the solution of gallic acid and Denis-Folin reagent is 0.06 mg / 1.6 ml, respectively. For the quantitative determination of the amount of phenolic compounds for the aqueous extraction of sumac fluffy must be added 1.1 ml of Denis-Folin reagent, to alcohol-water extraction – 1.2 ml of Folin-Denis reagent. The maximum value of optical density in aqueous and alcohol-aqueous extractions is observed at a wavelength of 720 nm. In alcohol-water extraction, more phenolic compounds (A = 0.8683) pass into the alcoholic water than to the aqueous one (A = 0.6346). The method used is valid and can be used for quantitative determination of the amount of phenolic compounds in fruits of sumac fluffy. To evaluate the linearity, the regression equation is calculated y = 941.67x + 0.0725 and the correlation coefficient r = 0.9895. The concentration of phenolic compounds in alcohol-water extraction was determined from the calibration graph. The average content of the sum of the phenolic compounds in terms of the gallic acid in the alcohol- water extraction was 10.04 ± 0.17%. The relative standard deviation (RSD) is 2.61%.

Quantitative Determination of Gallic acid and Cyanidin-3-O-Glucoside within Sumac Extracts by HPLC-MS/MS

Planta Medica ◽  
2011 ◽  
Vol 77 (12) ◽  
Author(s):  
M Koşar ◽  
F Göger ◽  
N Kırımer ◽  
KHC Başer
Keyword(s):  
Quantitative Determination ◽  
Gallic Acid

scholarly journals Phytochemical screening and in vitro antibacterial, antifungal, antioxidant and antitumor activities of the red propolis Alagoas

2019 ◽  
Vol 79 (3) ◽  
pp. 452-459 ◽  
Author(s):  
F. R. G. Silva ◽  
T. M. S. Matias ◽  
L. I. O. Souza ◽  
T. J. Matos-Rocha ◽  
S. A. Fonseca ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Gallic Acid ◽  
Bioactive Metabolites ◽  
Diffusion Method ◽  
Total Phenolic ◽  
Phytochemical Screening ◽  
Dpph Method

Abstract The study aimed to evaluate the antimicrobial activity, antioxidant, toxicity and phytochemical screening of the Red Propolis Alagoas. Antimicrobial activity was evaluated by disk diffusion method. Determination of antioxidant activity was performed using the DPPH assay (1.1-diphenyl-2-picrylhydrazyl), FTC (ferric thiocyanate) and determination of phenolic compounds by Follin method. Toxicity was performed by the method of Artemia salina and cytotoxicity by MTT method. The phytochemical screening for the detection of allelochemicals was performed. The ethanol extract of propolis of Alagoas showed significant results for antimicrobial activity, and inhibitory activity for Staphylococcus aureus and Candida krusei. The antioxidant activity of the FTC method was 80% to 108.3% hydrogen peroxide kidnapping, the DPPH method showed an EC50 3.97 mg/mL, the content of total phenolic compounds was determined by calibration curve gallic acid, resulting from 0.0005 mg/100 g of gallic acid equivalent. The extract was non-toxic by A. salina method. The propolis extract showed high activity with a higher percentage than 75% inhibition of tumor cells OVCAR-8, SF-295 and HCT116. Chemical constituents were observed as flavonones, xanthones, flavonols, and Chalcones Auronas, Catechins and leucoanthocyanidins. It is concluded that the extract can be tested is considered a potential source of bioactive metabolites.

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