scholarly journals Antidiabetic Activity of Extracts of Pithecellobium dulce Benth Leaves in Alloxan Induced Diabetic Rats

2016 ◽  
Vol 8 (05) ◽  
Author(s):  
V. S. Mul ◽  
N. Naikwade ◽  
C. Magdum ◽  
V. Jagtap
Keyword(s):  
Uric Acid ◽  
Blood Glucose ◽  
Total Cholesterol ◽  
Ethanolic Extract ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
Antidiabetic Activity ◽  
Control Group ◽  
Albino Rats ◽  
Glycogen Level

The objective of the present study was to study effect of Pithecellobium dulce Benth (P. dulce) leaves in alloxan induced diabetic rats. The P. dulce leaves were extracted by maceration and soxhelation method by using water and ethanol as solvent. Acute toxicity study was performed according to OECD 425 guidelines for both aqueous and ethanolic extracts of P. dulce leaves. The dose of 200 mg/kg and 400 mg/kg was selected for further studies. Animals were rendered diabetic by administration of alloxan (130 mg/kg, i.p.). The albino rats were divided in to seven groups with five animals in each group. Diabetic animals were treated with aqueous and ethanolic extract for 20 days. Then blood glucose, triglyceride, total cholesterol, urea, uric acid, creatinine, aspartate aminotransferase (AST), alanine transaminase (ALT) and glycogen level in liver, muscle and kidney were estimated according to standard procedures. The result shows significant decrease in blood glucose, triglyceride, total cholesterol, urea, uric acid, creatinine, AST and ALT level when compared to diabetic group. The liver and muscle glycogen level was increased significantly in extract treated groups when compared to diabetic control group. Both extract of P. dulce posses antidiabetic and hypolipidemic potential.

scholarly journals EFFECT OF PITHECELLOBIUM DULCE BENTH LEAVES IN DEXAMETHASONE INDUCED DIABETIC RATS

2016 ◽  
Vol 8 (9) ◽  
pp. 317 ◽  
Author(s):  
Mule V. S. ◽  
Naikwade N. S. ◽  
Magdum C. S. ◽  
Jagtap V. A.
Keyword(s):  
Blood Glucose ◽  
Total Cholesterol ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
Antidiabetic Activity ◽  
Control Group ◽  
Albino Rats ◽  
Glycogen Level ◽  
Standard Drug ◽  
Diabetic Control Group

<p><strong>Objective: </strong>The objective of the present study was to study the effect of <em>Pithecellobium dulce </em>Benth (<em>P. dulce</em>) leaves in dexamethasone-induced diabetic rats.</p><p><strong>Methods: </strong>The authenticated <em>P. dulce</em> leaves were collected from a local area of Sangli, Maharashtra. The leaves of the plant were extracted with water and ethanol by maceration and soxhelation respectively. Acute toxicity studies of the both extracts were performed using rat and according to OECD 425 guidelines. The dose of 200 mg/kg and 400 mg/kg was selected for further studies. The albino rats were divided into seven groups with five animals in each group. The diabetes was induced by dexamethasone (10 mg/kg, s. c.) and treated with extract and standard drug for 10 d. Then blood glucose, triglyceride, total cholesterol and glycogen level in liver, muscle and kidney were estimated according to standard procedures.</p><p><strong>Results: </strong>The study revealed that <em>P. dulce</em> at 200 mg/kg and 400 mg/kg showed significant (p ˂ 0.05) antidiabetic activity. All the extract treated groups showed a significant reduction in blood glucose level on 11<sup>th</sup> day when compared to diabetic control group. The significant increase in blood glucose, triglyceride, and total cholesterol level was observed in the diabetic control group when compared to normal control group. The liver and muscle glycogen level was decreased significantly (p ˂ 0.05) in the diabetic control group.<strong></strong></p><strong>Conclusion: </strong>It can be concluded that <em>P. dulce</em>aqueous and ethanolic extract at two different doses (200 mg/kg and 400 mg/kg) possesses antidiabetic and hypolipidemic activity.<p> </p>

scholarly journals PRELIMINARY PHYTOCHEMISTRY AND ANTIDIABETIC ACTIVITY OF PORTULACA GRANDIFLORA HOOK PLANT EXTRACT ON STREPTOZOTOCIN-INDUCED DIABETES IN RATS

2019 ◽  
pp. 87-90
Author(s):  
DEVI M ◽  
KOMAL S ◽  
LOGESHWARI B
Keyword(s):  
Blood Glucose ◽  
Ethanolic Extract ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
Antidiabetic Activity ◽  
Albino Rats ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Portulaca Grandiflora ◽  
Streptozotocin Induced Diabetes

Objective: The present study was aimed to evaluate the antidiabetic activity of ethanolic extract of the whole aerial plant of Portulaca grandiflora Hook on streptozotocin (STZ)-induced diabetic rats. Methods: Experimental diabetes was induced by a single dose of intraperitoneal injection of STZ (150 mg/kg). Adult male Wister albino rats were divided into five groups; normal control, diabetic control, diabetic glibenclamide (5 mg/kg), diabetic P. grandiflora H. extract (200 mg/kg), and diabetic P. grandiflora H. extract (400 mg/kg) for 21 days and analyzed for body weight (BW) and blood glucose. Results: The STZ-treated diabetic control rats showed a significant increase in blood glucose with a concomitant decrease in BW. Oral administration of P. grandiflora H. extract (200 and 400 mg/kg) and glibenclamide (5 mg/kg) for 21 days showed a significant reduction in blood glucose levels and elevation in the bodyweight studies as compared to control and glibenclamide-treated rats. Conclusion: The results of the present study showed that a potent antidiabetic activity was present in the aerial part of plant P. grandiflora H. extract.

scholarly journals Evaluation of Antidiabetic Activity of Polyherbal Formulation “Vasant Kusumakar Ras” on Alloxan-induced and Dexamethasone-induced Diabetic Rats

2021 ◽  
pp. 13-22
Author(s):  
Talha Jawaid ◽  
Kumari Nishu ◽  
Mehnaz Kamal ◽  
Saud M. Alsanad
Keyword(s):  
Blood Glucose ◽  
Group Treatment ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
Antidiabetic Activity ◽  
Control Group ◽  
Albino Rats ◽  
Reference Drug ◽  
Polyherbal Formulation ◽  
Diabetic Control Group

Aim: The current study observed the antidiabetic effect of Vasant Kusumakar Ras, an Ayurvedic polyherbal formulation, in alloxan-induced and dexamethasone-induced diabetic rats. Materials and Methods: Alloxan (120 mg/kg, i.p.) and dexamethasone sodium phosphate (5 mg/kg, i.p.) were used to induce diabetes in rats. The oral antidiabetic activity of Vasant Kusumakar Ras was evaluated by single doses of Vasant Kusumakar Ras (400 and 600 mg/kg, p.o.) in albino rats during a 10-day treatment period, with the effect of the Vasant Kusumakar Ras on blood glucose levels and serum lipid parameters measured on 0, 7th, and 11th day. Glibenclamide (5 mg/kg, p.o.) was used as the reference drug. Results: In alloxan-induced diabetic rats, the elevated levels of blood glucose significantly (p < 0.05) decreased after oral administration of Vasant Kusumakar Ras (400 mg/kg and 600 mg/kg), and Glibenclamide (5 mg/kg). When compared to the diabetic control group, treatment with Vasant Kusumakar Ras and Glibenclamide for 10 days reduced total cholesterol (TC) significantly (p < 0.001). Treatment with Vasant Kusumakar Ras and Glibenclamide for 10 days, significantly (p < 0.001) decreased low-density lipoprotein (LDL) level when compared to the diabetic control group. In dexamethasone-induced diabetic rats, all rats given with dexamethasone and Vasant Kusumakar Ras (400 mg/kg and 600 mg/kg) showed a significant (p < 0.05) decrease in the level of blood glucose when compared with diabetic control rats. The rats treated with dexamethasone and Glibenclamide showed a significant (p < 0.05) decrease in blood glucose level when compared to diabetic control rats. When compared to the diabetic control group, treatment with Vasant Kusumakar Ras and Glibenclamide (5 mg/kg) for 10 days reduced TC significantly (p < 0.001). Treatment with Vasant Kusumakar Ras and Glibenclamide for 10 days, significantly (p < 0.001) decreased LDL level when compared to the diabetic control group. Conclusion: Vasant Kusumakar Ras was shown to have significant antidiabetic activity comparable to that of glibenclamide and it also improves the lipid metabolism in both alloxan-induced and dexamethasone-induced diabetic rats.

scholarly journals Antidiabetic Activity ofAcacia tortilis(Forsk.) Hayne ssp. raddiana Polysaccharide on Streptozotocin-Nicotinamide Induced Diabetic Rats

2014 ◽  
Vol 2014 ◽  
pp. 1-9 ◽  
Author(s):  
Pradeep Kumar Bhateja ◽  
Randhir Singh
Keyword(s):  
Blood Glucose ◽  
Blood Glucose Level ◽  
Total Cholesterol ◽  
Glucose Level ◽  
Fasting Blood Glucose ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
Antidiabetic Activity ◽  
Control Group ◽  
Fasting Blood Glucose Level

The present study was designed to investigate the antidiabetic activity of aqueous extract ofAcacia tortilispolysaccharide (AEATP) from gum exudates and its role in comorbidities associated with diabetes in STZ-nicotinamide induced diabetic rats. Male albino Wistar rats were divided into control, diabetic control, glimepiride treated (10 mg/kg), and diabetic rats treated with 250, 500, and 1000 mg/kg dose of AEATP groups and fasting blood glucose, glycated hemoglobin, total cholesterol, triglyceride, LDL, VLDL, HDL, SGOT, and SGPT levels were measured. STZ significantly increased fasting blood glucose level, glycated hemoglobin, total cholesterol, triglyceride, LDL, VLDL, SGOT, and SGPT levels, whereas HDL level was reduced as compared to control group. After 7 days of administration, 500 and 1000 mg/kg dose of AEATP showed significant reduction(P<0.05)in fasting blood glucose level compared to diabetic control. AEATP has also reduced total cholesterol, triglyceride, LDL, VLDL, SGOT, and SGPT levels and improved HDL level as compared to diabetic control group. Our study is the first to report the normalization of fasting blood glucose level, lipid profile, and liver enzyme in AEATP treated diabetic rats. Thus, it can be concluded that AEATP may have potentials for the treatment of T2DM and its comorbidities.

scholarly journals Hypoglycemic Potential of Dietary Supplementation of Protein Isolate from Fermented Cucumeropsis manii in Streptozotocin Induced Hyperglycemic in Male Wistar Albino Rats

2021 ◽  
pp. 31-42
Author(s):  
A. O. Abiola ◽  
A. O. Iyoribhe ◽  
S. A. Adeniyi ◽  
O. B. Adu ◽  
A. S. Ogunbowale ◽  
...  
Keyword(s):  
Blood Glucose ◽  
Blood Glucose Concentration ◽  
Diabetic Control ◽  
Protein Isolate ◽  
Diabetic Rats ◽  
Control Group ◽  
Albino Rats ◽  
Antioxidant Enzyme Activities ◽  
Cvd Risk ◽  
Streptozotocin Induced Diabetes

The effect of Protein isolate from fermented melon seeds (Ogiri Protei Isolates; OPI) of Cucumeropsis manii on blood glucose, lipid profile, and antioxidant enzyme activities in streptozotocin (STZ)-induced diabetic rats was investigated. Thirty Male Wistar rats were divided into five equal groups. GThe first control group with no exposure. The second group of rats with Streptozotocin-induced non-treated diabetes. The 3rd and 4th groups of rats with Streptozotocin-induced diabetes supplemented with Ogiri protein isolates (200, 600 mg/kg in diet). And the 5th group of rats with Streptozotocin-induced diabetes administered glibenclamide in a dose 500 ug/kg in diet [17]. The OPI was administered for 6 weeks. The administration of OPI reduced the blood glucose concentration of the STZ-induced diabetic rats. Sera and hepatic superoxide dismutase, activities of the STZ-induced diabetic rats were significantly (P< 0.05) increased in comparison with the diabetic control rats. Lipid peroxidation of the supplemented OPI diabetic rats was significantly (P< 0.05) decreased in comparison with the diabetic control rats as the administration of OPI to the STZ-induced diabetic rats significantly increased the enzymes’ activities. The concentration of low-density lipoproteins in the OPI supplemented rats was significantly elevated. These data demonstrate that OPI supplements might be beneficial for correcting hyperglycemia but the consumption of OPI can modulate some tissue lipids in a direction not beneficial for CVD risk in patients with diabetes.

scholarly journals BLOOD GLUCOSE LEVELS AND THE MICROSCOPIC STRUCTURE OF KIDNEY WISTAR RAT DIABETES MELLITUS UNDER THE EFFECT OF LAWSONIA INERMIS (LINN.) LEAVES ETHANOLIC EXTRACT

2018 ◽  
Vol 11 (4) ◽  
pp. 257
Author(s):  
Mutiara Indah Sari ◽  
Maya Anjelir Antika ◽  
Dwi Rita Anggraini
Keyword(s):  
Diabetes Mellitus ◽  
Blood Glucose ◽  
Ethanolic Extract ◽  
Wistar Rat ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
Control Group ◽  
Lawsonia Inermis ◽  
Control Group Design ◽  
Glucose Levels

 Objective: Lawsonia inermis (Linn.) leaves are one of the alternative medicines to treat diabetes mellitus in Indonesia. We investigated the blood glucose level (BGL) of the L. inermis (Linn.) leaves ethanolic extract (LLEE) leaves and evaluated the histopathological alterations in diabetic rats.Methods: This study was an experimental study with posttest - only control group design. Alloxan (120 mg/kg, intraperitoneally)-induced diabetic rats. 35 of Wistar rats (Rattus norvegicus) were divided randomly into five groups, i.e. K: Normal control, P1: Diabetic control, and P2, P3, and P4 (200 mg/kg body weight [BW], 400 mg/kg BW, and 600 mg/kg BW of LLEE, orally) for 28 days. At the end of the treatment, the rats were sacrificed to obtain the kidney for histopathological evaluation using hematoxylin and eosin technique. BGLs were conducted using a glucose meter (GlucoDR).Results: One-way ANOVA test showed that dose 400 mg/kg BW of the LLEE was related to BGL of alloxan-induced diabetic rats (p=0.000). The histopathological of kidney showed glomerular inflammation (GI), epithelial membrane lining degeneration, vascular congestion, and interstitial tubule hemorrhage at diabetic control (P1). Meanwhile, treated with 600 mg/kg BW of LLEE (P4) showed increase cellular regeneration as normal architecture of the kidney.Conclusion: The LLEE at dose 400 mg/kg BW effective decreased BGL and was able to restore the kidney destruction of alloxan-induced diabetic rats at dose 600 mg/kg BW.

scholarly journals Comparative study of the effect of ethanolic extract of swietenia mahagoni seeds with rosiglitazone on experimentally induced diabetes mellitus in rats

2013 ◽  
Vol 39 (1) ◽  
pp. 6-10
Author(s):  
SMMMA Hasan ◽  
MI Khan ◽  
BU Kumar ◽  
MZ Sadeque
Keyword(s):  
Blood Glucose ◽  
Ethanolic Extract ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
Control Group ◽  
Diabetic Control Group ◽  
Group B ◽  
Group D ◽  
Swietenia Mahagoni ◽  
Experimentally Induced

The study was performed to compare the blood glucose lowering effect of Swietenia mahagoni seeds with an oral antidiabetic drug, rosiglitazone in experimentally induced diabetic rats. Twentyfour healthy Long Evans Norwegian strain of rats were included in the study and divided into four groups (A, B, C and D) comprising 6 rats each. Group A (control group) received standard rat food for 14 days. Diabetes was induced by a single intraperitoneal administration of alloxan 120mg/kg body weight in Group B, C and D. Group B was given standard food for 10 days and considered as diabetic control. Group C and D were treated with ethanolic extract of Swietenia mahagoni seeds 1000mg/kg and rosiglitazone 10mg/kg orally respectively. Administration of ethanolic extract of Swietenia mahagoni seeds in group C and rosiglitazone in group D produced a significant reduction in blood glucose level as compared to diabetic control (group B). Histological examination of pancreas showed destruction of beta cells in Islets of pancreas in group B whereas retaining of islets and few degranulations of beta cells of pancreas found in group C and group D. The observations and results of the present study provide information that ethanolic extract of Swietenia mahagoni seeds has hypoglycaemic effect in experimentally induced diabetic rats which requires further investigation. DOI: http://dx.doi.org/10.3329/bmrcb.v39i1.15790 Bangladesh Med Res Counc Bull 2013; 39: 6-10

scholarly journals Hypoglycaemic and Hypolipidemic Effects of Treculia africana Aqueous Leaves Extract in Alloxan Induced Diabetic Rats

2018 ◽  
pp. 1-9
Author(s):  
Idris A. Kankara ◽  
Gayus A. Paulina ◽  
M. Aliyu
Keyword(s):  
Body Weight ◽  
Blood Glucose ◽  
Total Cholesterol ◽  
Ldl Cholesterol ◽  
Fasting Blood Glucose ◽  
Hdl Cholesterol ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
Albino Rats ◽  
Treculia Africana

This study investigated the hypoglycaemic and hypolipidaemic effects of Treculia africana plant used in Nigeria as medicinal plant. Diabetes mellitus was induced by a single dose intraperitoneal injection of alloxan 150 mg/kg body weight. Twenty five (25) male albino rats were divided into five groups, five (5) rats per group; normal control, diabetic control and diabetic groups treated with aqueous leaves extract of 200,400 and 800 mg/Kg body weight respectively for 21 days orally. The effects of the extract on some biochemical parameters were evaluated; fasting blood glucose level was assayed using glucose oxidase method, total cholesterol and HDL –cholesterol were assayed using enzymatic method while LDL- cholesterol was determined by Friedewald equation. The results showed that, extract significantly (p<0.05) decrease the elevated fasting blood glucose levels, total cholesterol, triglyceride and LDL- cholesterol when compared with the diabetic control rats. The extract also caused significant (p<0.05) increased in HDL –cholesterol and body weight when compared with diabetic control rats. Aqueous leave extract of Treculia africana possess hypoglycemic effect and the most effective dose was 800 mg/Kg body weight in amelioration of hyperglycaemia and most all toxicity effects of alloxan on lipid profile.

scholarly journals ANTIDIABETIC AND ANTIHYPERLIPIDAEMIC EFFECTS OF THE ETHANOL EXTRACT OF THE LEAVES AND STEM OF CISSUS GRACILLIS (GULL ET PERR) (VITACEAE)

2021 ◽  
pp. 54-56
Author(s):  
OLUSAYO A SHORINWA ◽  
GORDON EI EMENU
Keyword(s):  
Blood Glucose ◽  
Ethanol Extract ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
Control Group ◽  
Albino Rats ◽  
Phytochemical Screening ◽  
Glucose Levels ◽  
Diabetic Control Group ◽  
Alloxan Monohydrate

Objectives: This study investigated the antidiabetic and antihyperlipidemic potential of the ethanol extract of the leaves and stem of Cissus gracillis on alloxan monohydrate-induced diabetic albino rats. Methods: Preliminary phytochemical screening and acute toxicity were carried out. Animals were assigned into seven groups of five rats each. Groups A and B were administered 10 mg/kg each of glibenclamide and atorvastatin respectively, C, D, and E were given 125, 250 and 500 mg/kg of ethanol extract of C. gracillis, respectively, daily for 21 days through oral gavage, group F was diabetic but untreated (diabetic control group), while group G was non-diabetic and untreated which served as the control group. Results: Phytochemical screening revealed the presence of steroids/triterpenoids and carbohydrates. LD50 was above 5000 mg/kg. The extract at 500 mg/kg showed a statistically significant (p<0.05) decrease in blood glucose level when compared with the glibenclamide group on day 21. However, gradual non- significant reduction in blood glucose levels were observed in the extract treated groups on the 7th, 14th, and 21st days of treatment. The administration of ethanol extract of C. gracillis to alloxan-induced diabetic rats produced a decrease in total cholesterol, triglycerides, and low-density lipoproteins comparable to glibenclamide and atorvastatin. Conclusion: The ethanol extract of the leaves and stem of C. gracillis possess a mildly significant antidiabetic and antihyperlipidemic activity.

scholarly journals Anti-hyperglycemic Effects of Diacerein in Alloxan-Induced Diabetes Mellitus in Wistar Albino Rats

2020 ◽  
pp. 107-113
Author(s):  
Arsalan Uqaili ◽  
Samia Siddiqui ◽  
Roomi Aijaz ◽  
Yar Muhammad Nizammani ◽  
Navaid Kazi ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Body Weight ◽  
Blood Glucose ◽  
Treated Group ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
Control Group ◽  
Albino Rats ◽  
Group B ◽  
Group D

Objective: To determine the anti-hyperglycemic effects of interleukin-1 inhibitor (diacerein) in alloxan induced diabetic albino wistar rats. This experimental study was performed at the Department of Animal Husbandry and Veterinary Sciences, Sindh Agriculture University, Tando Jam within 6 months from April 2016 to September 2016. Total of 160 adult Albino Wistar Rats having an average of 200 to 300 grams body weights were selected. Animals were categorized into 4 groups as; Group A (n=15): Control rats – receive 0.9% normal saline as placebo Experimental Groups Group B (n=15): Experimental Control (Diabetic rats) - Alloxan50 mg/kg body weight intraperitoneal. Group C (n=15): Diabetic rats + Diacerein (30 mg/kg/day) orally daily. Group D (n=15): Diabetic rats + Diacerein (50 mg/kg/day) orally daily. Animals were kept and treated as per the NIH Guideline for Use and Care of Laboratory Animals. Diabetes mellitus was induced via a single intraperitoneal injection of 50 milligram/kg alloxan monohydrated dissolved in aseptic 0.9% saline. After 72 hours, blood specimens were taken from the caudal vein of the rats and glucose level>200 mg/dL was taken as diabetes. Experimental rats were given diacerein approximately 30 and 50 mg orally for 6 weeks. At the completion of experiment the body weight was measured of each animal by electronic measuring balance and blood sample was taken from each animal of all groups to assess the blood glucose level and HbA1c level. Data were recorded via self-made proforma and analysis was done by using SPSS version 20. Results: Average body weight of Diabetic control (Group B) was 193.33±22.50 grams, which was lower in contrast to Diacerein treated group C 202.47±25.70 grams and significantly lower as compared to Diacerein treated group D as  212.6±23.43 grams. A significant increase in blood glucose levels 182.07±10.63 mg/dl was noted in the Diabetic control (Group B) compared to Diacerein treated group C (110.13± 8.54 mg/dl) and group D (85.87±8.41 mg/dl) (P=0.001). HbA1c was markedly raised in the Group B- diabetic controls, while diacerein treated diabetic rats (groups C and D) showed a significant decrease in HbA1c (P=0.001). Conclusion: It was concluded that Diacerein achieves the Euglycemic state by reducing the levels of blood glucose and glycated hemoglobin (HbA1c) in Alloxan-Induced diabetes mellitus in Wistar Albino Rats.

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