scholarly journals Immunological and hematological changes in patients with hyperthyroidism or hypothyroidism

2010 ◽  
Vol 33 (5) ◽  
pp. 271 ◽  
Author(s):  
Abdollah Jafarzadeh ◽  
Masoud Poorgholami ◽  
Nazanin Izadi ◽  
Maryam Nemati ◽  
Mohammad Rezayati
Keyword(s):  
Eosinophil Count ◽  
Complete Blood Count ◽  
Hematological Parameters ◽  
White Blood Cells ◽  
Serum Levels ◽  
Control Group ◽  
Complement Components ◽  
The Mean ◽  
Hematological Changes ◽  
Hypothyroid Patients

Aims: Thyroid hormones have been shown to influence the immune system and haematopoiesis. The aim of this study was to evaluate some immunological and hematological parameters in peripheral blood of hypo- or hyperthyroid women. Materials and Methods: Blood samples were collected from 50 women with hypothyroid disease, 50 women with hyperthyroid disease and a control group consisting of 50 sex - and age - matched euthyroid subjects. Thyroid function assesed according to measurent of T3, T4 and TSH levels. The complete blood count (CBC), total and differential counts of white blood cells (WBC), serum levels of immunoglobulins (IgG, IgA, IgM and IgE) and C3 and C4 complement components determined in three groups by using standard immunological and hematological methods. Results: In hyperthyroid women the mean serum concentrations of IgG (2312.4±584 mg/dl), IgA (296± 87 mg/dl) and IgE ( 301± 264 IU/ml) were significantly higher than those found in the control group (1539± 974 mg/dl, P < 0.0003; 234± 116 mg/dl, P < 0.01; 109.8±115 IU/ml, P < 0.0001, respectively) and the mean MCV was significantly lower in comparison with the euthyroid group (P < 0.05). Hypothyroid patients had higher serum IgE concentrations in comparison with the euthyroid group (179.8± 218 IU/ml vs. 109.8± 115 IU/ml; P < 0.047). The mean serum C3 concentration in hypothyroid patients was also significantly higher in comparison with the euthyroid group (138.7± 36.6 mg/ml vs. 117.8± 32.1 mg/dl; P < 0.01). In the hypothyroid group the mean eosinophil count was markedly higher in comparison with the hyperthyroid group (P < 0.06) and the mean count of RBC and the levels of some RBC-related indices, such as hematocrit and hemoglobin, were significantly lower in comparison with the euthyroid group (P < 0.05). Conclusion: These results indicate hypergammablobulinemia and lower MVC in hyperythyroid patients, and higher IgE levels, C3 levels and eosinophil count as well as anemia in hypothyroid patients.

Serum Osteopontin Growth hormone , and Other Biochemical Parameters in Children with Acute Lymphoblastic Leukemia

2011 ◽  
Vol 7 (1) ◽  
pp. 1169-1174 ◽  
Author(s):  
Atheer A. Mehde ◽  
Wesen A. Mehdi ◽  
Karima F. Ali
Keyword(s):  
Growth Hormone ◽  
Acute Lymphoblastic Leukemia ◽  
Biochemical Parameters ◽  
Lymphoblastic Leukemia ◽  
White Blood Cells ◽  
Serum Levels ◽  
Significant Negative Correlation ◽  
Control Group ◽  
Surface Receptors ◽  
The Mean

Acute lymphoblastic leukemia (ALL) is a custom of leukemia, or cancer of the white blood cells categorized by excess lymphoblasts and Chemotherapy is the initial treatment of choice. Most ALL patients will receive a combination of different treatments.Osteopontin (OPN) is a glycoprotein that cooperates with a variety of cell surface receptors, as well as several integrins and CD44. OPN is secreted by numerous human malignancies. We aimed to evaluate serum levels of osteopontin and growth hormone(GH) in patients with Acute lymphoblastic leukemia in childhood. We studied serum levels of OPN ,GH and other biochemical levels of 60 patients that diagnosis as  acute lymphoblastic leukemia.      The mean levels of OPN were significantly higher in patients (10.00± 1.15 ng/ml) than in control (7.62± 0.60 ng/ml). The mean levels of GH levels were significantly lower in patients (2.98± 0.51ng/dl) than in control  (6.02± 1.00 ng/dl. The mean levels of the iron and total protein  in ALL patients  were (95.78± 10.11 µg/dl), (6.08 ± 1.16 g/dl)respectively. The present study  showed a significant  negative correlation between GH with  osteopontin (OPN) ,while there a positive significant  correlate  between GH with  hemoglobin  in patients group compared to control group. The present study Conclude that elevated levels of osteopontin and decreased GH levels at the diagnosis may predict an unfavorable result in  ALL childhood .

scholarly journals Hematological and Oxidative Status Parameters in Domestic Dogs Naturally Infested by Rhipicephalus Sp.

2020 ◽  
Vol 43 (2) ◽  
pp. 103-110
Author(s):  
Rosa Kebbi ◽  
Omar Besseboua ◽  
Melaaz Belhadj-Kebbi ◽  
Lila Hassissen ◽  
Abdelhanine Ayad
Keyword(s):  
Blood Cells ◽  
Hematological Parameters ◽  
White Blood Cells ◽  
Serum Levels ◽  
Oxidative Status ◽  
Domestic Dogs ◽  
Significant Difference ◽  
Hematological Analysis ◽  
Brachial Vein ◽  
The Mean

AbstractThe present study was aimed to evaluate hematological and oxidative stress parameters in domestic dogs infested naturally (n=10) by Rhipicephalus sp. to compare with non-infested dogs (n=10). All blood samples were collected from brachial vein into tubes EDTA for the hematological analysis such as red blood cells (RBCs), white blood cells (WBCs), hemoglobin (HGB) and platelets (PLT). Serum was rapidly separated after centrifugation and stored at -20 °C until it was used for malondialdehyde (MDA) and 2,2’-Azinobis-(3-ethylbenzothiazoline)-6-sulfonic acid (ABTS) inhibition measurements. HGB in non-infested dogs was significantly higher than in infested dogs (P<0.05). There was no significant difference in RBCs, WBCs and PLT between both groups (P>0.05). The mean of MDA concentration was high in infested dogs (0.92±0.62 nmol/ml) compared to non-infested dogs (0.75±0.25 nmol/ml). On the other hand, the percentage of ABTS inhibition was similar in both groups (P=0.71). High tick number seems significantly affected WBCs (P<0.0001) and HGB (P<0.001) in infested dogs. Concerning oxidative status, there was no significant differences (P>0.05) between low and high infested dogs, neither in the amount of MDA nor in the ABTS inhibition. In conclusion, infested dogs induced RBCs alterations, which coincided with the oxidative damage, as evidenced by MDA serum levels. Also, there was a relationship between the tick number in infested dogs and the hematological parameters.

scholarly journals The Importance of Antioxidant Status in Gastric Intestinal Metaplasia

2021 ◽  
Author(s):  
Nilay Danis ◽  
Aysegul Ertinmaz Ozkan ◽  
Fatih Karatas ◽  
Cagri Cakici ◽  
Turkan Yigitbasi ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Intestinal Metaplasia ◽  
Prognostic Marker ◽  
Smoking Status ◽  
Serum Levels ◽  
Control Group ◽  
Gastric Intestinal Metaplasia ◽  
Oxidative Stress Status ◽  
Cancer Types ◽  
The Mean

Background and Aim: Oxidative stress status in different cancer types was investigated before, but not studied in gastric intestinal metaplasia to the best of our knowledge. Purpose of this study is to examine whether there is a difference between oxidative stress status in patients with intestinal metaplasia (IM) compared to individuals without IM, we compared the serum levels of disulfide (SS), total thiol (TT) and native thiol (NT). Patients and Methods: This was a prospective, non-randomized case-control study including 67 patients with histopathologically confirmed IM and 60 individuals demographically matched in terms of age, gender, BMI, smoking status, and chronic diseases as control group. Results: The mean NT, TT and NT to TT(NT/TT) ratios were statistically significantly higher in IM group compared to controls [(351.71±81.9µmol/L vs. 271.82±54.13µmol/L, p=0.000),(391.5±92.69µmol/L vs. 308.59±55.53 µmol/L, 0.000) and (0.89±0.6 vs. 0.87±0.29,  p=0.022), respectively].The mean SS to TT(SS/TT) ratio was significantly lower in IM group than control group (0.050±0.31 vs. 0.060±0.014, P=0.022). Median SS and mean SS/NT ratio was similar in both groups[16.3 (3.3 – 78) vs. 18.3 (10 – 32.7), p=0.271 and 0.055 ± 0.041 vs. 0.070 ± 0.019, p=0.068, respectively). In ROC analysis, cut off value of SS/NT for IM was found 0.062, in regression analysis, SS/NT<0.062 was found as an independently prognostic marker for IM (OR, 2.38; 95%CI: 1.168 - 4.865, P=0.017). Conclusion: SS /NT ratio lower than 0.062 was found as an independently prognostic marker for IM. This ratio could help to distinguish which patients should be followed closely for gastric cancer.

scholarly journals An Investigation of the Relationship between Involvement Sites in Vitiligo Patients and Autoimmune Diseases and Hematological Parameters

2019 ◽  
Vol 4 (10) ◽  
Author(s):  
Ebru KARAGUN
Keyword(s):  
Hematological Parameters ◽  
Fasting Blood Glucose ◽  
Unknown Etiology ◽  
Control Group ◽  
Endocrine Disorders ◽  
Positive Correlation ◽  
Lymphocyte Ratio ◽  
History Of ◽  
The Mean ◽  
The Relationship

Aim-Objectives: Vitiligo is an acquired idiopathic disease which progresses with melanocyte destruction and is clinically characterized by depigmented lesions of unknown etiology. Vitiligo may be coexistence with a autoimmune and endocrine disorders. This study examined the sT3, fT4, TSH, Anti-TPO, Anti-Tg, Vitamin B12 and fasting blood glucose (FBG) values, and thrombocyte-to-lymphocyte ratio(TLR), neutrophil-to-lymphocyte ratio(NLR), the mean platelet volume(MPV) the correlation of depigmented lesions with the extent of body involvement sites(IS). Materials and Method: The study enrolled 67 patients aged 0‒65 who were diagnosed with non-segmental generalized vitiligo and in whom an increase in lesions had been observed in the last six months. The IS of the lesions in the patients were evaluated as IS ˂10%(1st group), 10%‒20% (2nd group), 20% - 30%(3rd group), 30% - 40%(4th group), 40% ‒50 %(5th group), and ˃50%(6th group). The control group consisted of patients who had presented to the outpatient clinic having had no history of vitiligo detected in themselves nor in their families. Results: No significant correlation was found between IS and sT3, fT4, TSH, Anti-TPO, Anti-Tg, Vit. B12, PBG or MPV. A moderately positive correlation was found between IS and duration (p <0.05) and a mildly positive correlation between IS and NLR and TLR (p <0.05). Conclusion: This study show that every patient diagnosed with vitiligo, independent of the IS, should undergo examination for autoimmune disease. A mild positive correlation between VTA and NLO-TLO was found to be an indicator of increased inflammation in vitiligo patients as the extent of lesions increased.

Immature Platelet Fraction in Patients with Chronic Liver Disease, A Marker for Evaluating Cirrhotic Changes.

2021 ◽  
Vol 17 (2) ◽  
pp. 174-179
Author(s):  
Muhammad Javaid Iqbal ◽  
Muhammad Usman ◽  
Mubarak Ali Anjum ◽  
Yasir Yaqoob ◽  
Ghulam Mujtaba Nasir ◽  
...  
Keyword(s):  
Platelet Count ◽  
Liver Disease ◽  
Chronic Liver Disease ◽  
White Blood Cells ◽  
Chronic Liver ◽  
Control Group ◽  
P Value ◽  
Master Program ◽  
Immature Platelet Fraction ◽  
The Mean

Objective: To evaluate the role of Immature platelet fraction in patients with chronic liver disease, a marker for evaluating cirrhotic changes. Methodology: This case control study was conducted at department of Pathology, Aziz Fatima Medical and Dental College, Faisalabad, over a period of Seven months from June 2020 to January 2021. A total of 126 participants were included in the study consisting of 63 patients with chronic liver disease in group A and 63 participants without any known disease in group B as control. The IPF master program in combination with XE-2100 multiparameter automatic hematology analyzer was used to measure the immature platelet fraction. Ethylene diamine tetraacetic acid was used to collect the blood sample for IPF measurement and was maintained till analysis on room temperature. Ten repeated analyses, immediately and after 24 hours were done for reproducibility of IPF%. Results: The mean age of liver disease patients was 52.35 ± 13.64 years and in control group the mean age was 51.62 ± 11.27 years. There was no significant (p-value > 0.05) difference between both groups based on age and gender. The hemoglobin level and red cell count was found to be significantly (p-value < 0.05) reduced in cases group. While white blood cells count was comparable in both groups. The mean platelet count was significantly (p-value < 0.05) less in cases group (163.5 ± 90.4 vs 233.4 ± 54.5 (x10*3/µl). The mean value of immature platelet fraction (IPF%) was significantly (p-value < 0.05) raised in cases group (5.62 ± 2.92 vs 3.06 ± 1.87). The multivariate discriminant analysis (MDA) score showed a significant (p-value < 0.05) association with chronic hepatis as compared to other liver related diseases. Conclusions: In chronic liver disease patients, there is an inverse relationship between platelet count and IPF% with decreased platelet count and increased IPF%. The proposed MDA function can be used to identify the cirrhotic changes in liver disease patients.

scholarly journals Cardiovascular and hematologic effects produced by chronic treatment with etoricoxib in normotensive rats

2009 ◽  
Vol 24 (3) ◽  
pp. 206-210 ◽  
Author(s):  
Nilo César do Vale Baracho ◽  
Guilherme Pedrosa Guizelli ◽  
Beatriz Leone Carmello ◽  
Danielle de Souza Sanches ◽  
Felipe Moraes Costa Silva ◽  
...  
Keyword(s):  
Blood Cells ◽  
Chronic Treatment ◽  
Experimental Period ◽  
Control Group ◽  
Histopathological Changes ◽  
Study Results ◽  
Significant Difference ◽  
Hematological Effects ◽  
The Mean ◽  
Hematological Changes

PURPOSE: Evaluate the cardiovascular and hematological effects produced by chronic treatment with two dosis of etoricoxib in Wistar normotensive rats. METHODS: Thirty rats have been used and divided into one control group and two etoricoxib (10mg/kg and 30mg/kg) treatments groups for 60 days. The mean arterial pressure (MAP) was taken during the whole experimental period and at the end of this period, under anesthesia blood samples were taken, and further the withdrawn of the aorta, heart, brain, liver, and kidneys for the anatomopathologic study. RESULTS: The treatment with etoricoxib (30mg/Kg) produced a significant increase of the MAP from the 28th day of the experiment and from the platelets when compared to the control group and to the group treated with 10mg/Kg, besides producing a highly significant difference in hematocrit and in the red blood cells in relation to the control group. On the other hand the treatment with etoricoxib has not caused histopathological changes when compared to the control. CONCLUSION: These data show that the chronic treatment with etoricoxib leads to increase of the MAP, and to important hematological changes which seem to be associated to the hemoconcentration although not producing anatomopathological significant changes.

scholarly journals Within-subject and between-subject biological variation estimates of 21 hematological parameters in 30 healthy subjects

2018 ◽  
Vol 56 (8) ◽  
pp. 1309-1318 ◽  
Author(s):  
Abdurrahman Coşkun ◽  
Anna Carobene ◽  
Meltem Kilercik ◽  
Mustafa Serteser ◽  
Sverre Sandberg ◽  
...  
Keyword(s):  
Healthy Subjects ◽  
Complete Blood Count ◽  
Hematological Parameters ◽  
Hemoglobin Concentration ◽  
Biological Variation ◽  
Mean Corpuscular Hemoglobin ◽  
Mean Values ◽  
Performance Specifications ◽  
The Mean ◽  
Variance Homogeneity

Abstract Background: The complete blood count (CBC) is used to evaluate health status in the contexts of various clinical situations such as anemia, infection, inflammation, trauma, malignancies, etc. To ensure safe clinical application of the CBC, reliable biological variation (BV) data are required. The study aim was to define the BVs of CBC parameters employing a strict protocol. Methods: Blood samples, drawn from 30 healthy subjects (17 females, 13 males) once weekly for 10 weeks, were analyzed using a Sysmex XN 3000 instrument. The data were assessed for normality, trends, outliers and variance homogeneity prior to coefficient of variation (CV)-analysis of variance (ANOVA). Sex-stratified within-subject (CVI) and between-subjects (CVG) BV estimates were determined for 21 CBC parameters. Results: For leukocyte parameters, with the exception of lymphocytes and basophils, significant differences were found between female/male CVI estimates. The mean values of all erythrocyte-, reticulocyte- and platelet parameters differed significantly between the sexes, except for mean corpuscular hemoglobin concentration, mean corpuscular volume and platelet numbers. Most CVI and CVG estimates appear to be lower than those previously published. Conclusions: Our study, based on a rigorous protocol, provides updated and more stringent BV estimates for CBC parameters. Sex stratification of data is necessary when exploring the significance of changes in consecutive results and when setting analytical performance specifications.

scholarly journals Hematological parameters of the blood count in a healthy population of pregnant women in the Northwest of Morocco (Tetouan-M'diq-Fnideq provinces)

2018 ◽  
Vol 29 ◽  
Author(s):  
Saad Bakrim ◽  
Youssef Motiaa ◽  
Ali Ouarour ◽  
Azlarab Masrar
Keyword(s):  
Pregnant Women ◽  
Reference Values ◽  
Blood Count ◽  
Complete Blood Count ◽  
Hematological Parameters ◽  
Control Group ◽  
Mean Corpuscular Hemoglobin ◽  
Healthy Population ◽  
Corpuscular Hemoglobin ◽  
Significant Difference

Introduction: numerous biological parameters are physiologically modified during normal pregnancy, in particular hematology. The knowledge of these modifications of the maternal body by biologists and clinicians allows the screening of possible anomalies. In Morocco, the reference values of the complete blood count test for pregnant woman are missing, as are those specific to different trimesters of pregnancy. The aim of this study is to look for the reference values for healthy pregnant women of the Northwest region of Morocco, to compare them to those of non-pregnant women (control) and to those of the literature. Methods: blood samples were taken voluntarily from 3898 healthy pregnant women from 18 to 46 years old who presented themselves at the center of health Kalaa and at the service of gynecology obstetrics of the Provincial Hospital Center of M'diq (Morocco), for prenatal care. To establish the reference intervals of the CBC for non-pregnant women, a control group was constituted by 7035 healthy women from 18 to 50 years old selected according to the Moroccan law of blood donation. The CBC was measured on a Sysmex KX21N® analyzer. For each sample a systematic blood smear was done to determine the leukocyte differential. Results: a statistically significant difference between the pregnant women and control group was noted (p < 0.05) for all the hematological parameters: red blood cells, hematocrit, hemoglobin, mean corpuscular volume, mean corpuscular hemoglobin, mean corpuscular hemoglobin concentration, leukocytes, neutrophils, basophils, eosinophils, lymphocytes, monocytes, platelets and mean platelet volume. So, the comparison of the averages established between the first, second and third trimester of pregnancy showed the existence of a significant variation with regard to all the parameters of the CBC test looked for (p < 0.001). Conclusion: the present study provides additional baseline data for basic hematological parameters in healthy pregnant Moroccan women and concluded that pregnancy in women has the tendency to alter some hematological indices. For these reasons, there is an interest to take these modifications into account for optimal maternal and fetal medical care.

scholarly journals Relationship between CBC and ESR and duration of disease in patients with systemic lupus erythematosus (SLE)

2018 ◽  
Vol 13 (2) ◽  
pp. 41-46
Author(s):  
Mahmuda Abira ◽  
Qazi Shamima Akhter
Keyword(s):  
Systemic Lupus Erythematosus ◽  
Lupus Erythematosus ◽  
Complete Blood Count ◽  
Hematological Parameters ◽  
Significant Negative Correlation ◽  
Control Group ◽  
Systemic Lupus ◽  
Initial Manifestation ◽  
Cross Sectional ◽  
Duration Of Disease

Background: Systemic lupus erythematosus (SLE) is an autoimmune disease and is associated with considerable morbidity. Hematological abnormalities may be the initial manifestation. Duration may have impact on their hematological parameters. Objective: To observe the relationship of Complete Blood Count (CBC) and Erythrocyte sedimentation Rate(ESR) with duration of disease in patients with SLE. Methods: This cross sectional study was conducted in the Department of Physiology, Dhaka Medical College, Dhaka from July 2016 to June 2017. Total 60 subjects including both sexes were selected with age ranging from 18 to 55 years. Among them, 30 subjects with SLE were study group and 30 age matched healthy subjects were control group. Total count of RBC, WBC, Platelet and ESR were estimated by automated hematology analyzer. Statistical analysis was done by unpaired Students æt” test, Chi Square test and Pearson’s correlation coefficient (r) test. Results: In this study, ESR was significantly (p<0.001) higher and RBC,WBC and platelet counts were significantly (p<0.001) lower in SLE patients in comparison to that of control. On correlation analysis, RBC and WBC showed significant negative correlation with duration of disease in patients with SLE. Conclusion: This study concludes that CBC and ESR were altered in SLE patients and it was related to duration of disease. J Bangladesh Soc Physiol. 2018, December; 13(2): 41-46

scholarly journals Neutrophil-to-Lymphocyte, Monocyte-to-Lymphocyte and Platelet-to-Lymphocyte Ratios in Relation to Clinical Parameters and Smoking Status in Patients with Graves’ Orbitopathy—Novel Insight into Old Tests

2020 ◽  
Vol 9 (10) ◽  
pp. 3111
Author(s):  
Joanna Szydełko ◽  
Michał Litwińczuk ◽  
Magdalena Szydełko ◽  
Beata Matyjaszek-Matuszek
Keyword(s):  
Complete Blood Count ◽  
Smoking Status ◽  
White Blood Cells ◽  
Smoking Behavior ◽  
Control Group ◽  
Clinical Activity ◽  
Clinical Parameters ◽  
Monocyte Count ◽  
Graves Orbitopathy ◽  
Insight Into

Graves’ orbitopathy (GO) is an autoimmune disease with a chronic inflammatory background. Smoking behavior is the main environmental factor responsible for the transition of this major extra thyroidal manifestation of Graves’ disease (GD) from the subclinical to the overt form. Complete blood count-derived parameters are suggested to be novel inflammatory indices. The aim of this retrospective study was to investigate the association between neutrophil-to-lymphocyte (NLR), monocyte-to-lymphocyte (MLR), and platelet-to-lymphocyte ratios (PLR) with selected clinical parameters and smoking status in 406 GD patients with (n = 168) and without GO (n = 238). The control group consisted of 100 healthy individuals. The activity of GO was graded according to Clinical Activity Score. Significantly higher white blood cells (WBC), neutrophil, and NLR (p < 0.05) values were observed in GD patients with GO compared with those without GO. PLR values were significantly higher in GO patients than in the controls. WBC (6.81 ± 1.56 vs. 5.70 ± 1.23) and neutrophils (3.89 ± 1.06 vs. 3.15 ± 0.95) count was higher in active GO patients than in those with inactive GO. Positive correlation (p < 0.05) between CAS score and WBC, neutrophil and monocyte count, and NLR was found. Smoking was associated with higher WBC (p = 0.040), neutrophil (p = 0.049), PLR (p = 0.032) values. Multivariate analysis revealed that WBC, NLR may be risk factors for GO development. WBC, neutrophil, NLR and PLR values seem to be useful tools in the assessment of inflammation in GD.

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