Antioxidants Activity of Self-Nanoemulsifying Drug Delivery System on Dayak Onions Extract (Eleutherine palmifolia) using DPPH (2,2 difenil-1- picrylhydrazyl) Method

Dayak onions (Eleutherine palmifolia L. Merr) is one plant that has been proven to have benefits as an antioxidant. The Dayak extract is formulated in the self nano emulsifying drug delivery system (SNEDDS) because the extract has low water solubility. The aims this study to develop the SNEDDS formulation system by testing its antioxidant activity. We determined whether there was an increase in antioxidant activity when formulated in the form of SNEDDS or not. The results were then compared with a solution of Dayak onions extract without using SNEDDS. The obtained formula was the optimal result that has been done before using the D-optimal mixture design method. The results of the components consisted of 50 mg of Dayak extract, 10 % caprylic triglyceride as oil, 1% tween 80, and 6,60 % transcutol as a combination surfactant and 12,40 % propylene glycol as co-surfactant. Antioxidant activity testing was carried out using the DPPH (1,1-Diphenyl-2-picrylhydrazyl) method. The antioxidant test using the DPPH method was done with two samples, namely the extract solution and Dayak onion extract SNEDDS. We elaborated the research by using UV-VIS spectrophotometer. Each sample was made into five concentrations, namely 30 ppm, 60 ppm, 90 ppm, 120 ppm, and 150 ppm, and carried out three times replications. The results showed that the IC50 value in the Dayak onion extract solution was 227,19 ppm (very low), while for the SNEDDS solution for the Dayak onion extract the IC50 value obtained was 38,97 ppm (very strong). The analysis was carried out next using an independent T-Test to obtain the results. There was no significant difference between the extract solution and the SNEDDS solution of Dayak onion extract.

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Nanostructured ZnO as Multifunctional Carrier for a Green Antibacterial Drug Delivery System—A Feasibility Study

Nanomaterials ◽

10.3390/nano9030407 ◽

2019 ◽

Vol 9 (3) ◽

pp. 407 ◽

Cited By ~ 11

Author(s):

Federica Leone ◽

Roberta Cataldo ◽

Sara Mohamed ◽

Luigi Manna ◽

Mauro Banchero ◽

...

Keyword(s):

Drug Delivery ◽

Antimicrobial Activity ◽

Drug Delivery System ◽

Delivery System ◽

Antimicrobial Drug ◽

Diffraction Field ◽

Antibacterial Drug ◽

Physico Chemical ◽

Two Samples

The physico–chemical and biological properties of nanostructured ZnO are combined with the non-toxic and eco-friendly features of the scCO2-mediated drug loading technique to develop a multifunctional antimicrobial drug delivery system for potential applications in wound healing. Two nanostructured ZnO (NsZnO) with different morphologies were prepared through wet organic-solvent-free processes and characterized by means of powder X-ray diffraction, field emission scanning electron microscopy (FESEM), and nitrogen adsorption analysis. The antimicrobial activity of the two samples against different microbial strains was investigated together with the in vitro Zn2+ release. The results indicated that the two ZnO nanostructures exhibited the following activity: S. aureus > C. albicans > K. pneumoniae. A correlation between the antimicrobial activity, the physico–chemical properties (specific surface area and crystal size) and the Zn2+ ion release was found. Ibuprofen was, for the first time, loaded on the NsZnO carriers with a supercritical CO2-mediated drug impregnation process and in vitro dissolution studies of the loaded drug were performed. A successful loading up to 14% w/w of ibuprofen in its amorphous form was obtained. A preliminary drug release test showed that up to 68% of the loaded ibuprofen could be delivered to a biological medium, confirming the feasibility of using NsZnO as a multifunctional antimicrobial drug carrier.

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Preparation, characterization, and evaluation of antioxidant activity and bioavailability of a self-nanoemulsifying drug delivery system (SNEDDS) for buckwheat flavonoids

Acta Biochimica et Biophysica Sinica ◽

10.1093/abbs/gmaa124 ◽

2020 ◽

Vol 52 (11) ◽

pp. 1265-1274

Author(s):

Zhijuan Zhao ◽

Xiaodong Cui ◽

Xiaoli Ma ◽

Zhuanhua Wang

Keyword(s):

Drug Delivery ◽

Antioxidant Activity ◽

Drug Delivery System ◽

Delivery System ◽

Oral Bioavailability ◽

In Vitro Release ◽

Scavenging Activity ◽

Vitro Release

Abstract The self-nanoemulsifying drug delivery system has shown many advantages in drug delivery. In this study, a self-nanoemulsifying drug delivery system of buckwheat flavonoids was prepared for enhancing its antioxidant activity and oral bioavailability. A nanoemulsion of buckwheat flavonoids was developed and characterized, and its antioxidant, in vitro release, and in vivo bioavailability were determined. The nanoemulsion was optimized by the central composite design response surface experiment, and its particle size, polymer dispersity index (PDI), zeta potential, morphology, encapsulation efficiency, and stability were evaluated. The antioxidant activity was tested by measuring its 2,2-diphenyl-1-picrylhydrazyl scavenging activity, hydroxyl radical scavenging activity, and superoxide anion scavenging ability. In vitro release of buckwheat flavonoids nanoemulsion showed a higher cumulative release than the suspension, and the release fitting model followed the Ritger–Peppas and Weibull models. The effective concentration of the nanoemulsion was evaluated in vivo using a Wistar rat model, and the area under the plasma concentration-time curve of the buckwheat flavonoids nanoemulsion was 2.2-fold higher than that of the buckwheat flavonoid suspension. The Cmax of the nanoemulsion was 2.6-fold greater than that of the suspension. These results indicate that the nanoemulsion is a promising oral drug delivery system that can improve the oral bioavailability to satisfy the clinical requirements.

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Erratum to: Preparation, characterization, and evaluation of antioxidant activity and bioavailability of a self-nanoemulsifying drug delivery system (SNEDDS) for buckwheat flavonoids

Acta Biochimica et Biophysica Sinica ◽

10.1093/abbs/gmab062 ◽

2021 ◽

Author(s):

Zhijuan Zhao ◽

Xiaodong Cui ◽

Xiaoli Ma ◽

Zhuanhua Wang

Keyword(s):

Drug Delivery ◽

Antioxidant Activity ◽

Drug Delivery System ◽

Delivery System

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Folate-Conjugated Chitosan-Pluronic P123 Nanogels: Synthesis and Characterizations towards Dual Drug Delivery

Journal of Nanomaterials ◽

10.1155/2019/1067821 ◽

2019 ◽

Vol 2019 ◽

pp. 1-14

Author(s):

Van Toan Nguyen ◽

Thi Hương Nguyen ◽

Le Hang Dang ◽

Hieu Vu-Quang ◽

Ngoc Quyen Tran

Keyword(s):

Drug Delivery ◽

Anticancer Activity ◽

Drug Delivery System ◽

Delivery System ◽

Breast Cancer Cell Lines ◽

Uniform Size ◽

Pluronic P123 ◽

Ic50 Value ◽

Therapeutic Compounds ◽

Dual Drug

In this study, a self-assembled nanogel-based pluronic P123-grafted chitosan-folate (CP-FA) was fabricated as a paclitaxel/curcumin codelivery system. 1H-NMR and TGA proved that the fabricating method of CP-FA was successful. Dynamic light scattering (DLS), zeta potentials, and transmission electron microscopy (TEM) exposed that CP-FA nanoparticles had a uniform size with a diameter of around 16.27±2.01 nm in the colloidal solution and had better sustainable stability at a lower concentration than P123 due to the moderate positive potential value (39.43±3.45 mV) and the lower critical micelle concentration (0.036 mg/ml). Dual drugs were loaded with CP-FA nanogels via self-assembly by the hydrophobic interaction between both hydrophobic therapeutic compounds (PTX and Cur) and the hydrophobic segment of the P123 copolymer. The high hydrophobicity of the segment induced a great loading efficacy of up to 98.63±0.42 of PTX and 97.82±0.48 of Cur. In addition, the CP-FA nanogels exposed superior effects in a controlled release of these encapsulated therapeutic compounds for a long period of time. The anticancer activity of the dual-drug delivery system was evaluated using human breast cancer cell lines (MCF-7) via the IC50 value to compare with the PTX-loading CP-FA nanogel. The obtained results suggested that CP-FA/PTX-Cur displayed a remarkable improvement in anticancer activity at an IC50 value of 5.74±0.23 nM which was higher than that of CP-FA/PTX (IC50=8.20±1.41 nM) due to the synergistic effect of both PTX and Cur. Thereby, a dual-drug delivery-system-based CP-FA of PTX and Cur has been proposed as a promising candidate in cancer therapy.

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New heterocyclic compounds: synthesis, antioxidant activity and computational insights of nano-antioxidant as ascorbate peroxidase inhibitor by various cyclodextrin as drug delivery systems

Current Drug Delivery ◽

10.2174/1567201817999201001205627 ◽

2020 ◽

Vol 17 ◽

Author(s):

Yassine Kaddouri ◽

Farid Abrigach ◽

El Bekaye Yousfi ◽

Belkheir Hammouti ◽

Mohamed El Kodadi ◽

...

Keyword(s):

Drug Delivery ◽

Antioxidant Activity ◽

Drug Delivery System ◽

Binding Affinity ◽

Delivery System ◽

Reduction Method ◽

Antioxidant Activities ◽

Condensation Reaction ◽

Docking Study ◽

Antioxidant Agents

Aim: : The synthesis of seven new antioxidant agents based on the combination of thiazole, pyridine, triazole and pyrazole moieties. - The studies of their antioxidant activity using DPPH reduction method. - The DFT analysis of the 7 ligands. - The docking study was also investigated. - The better binding affinity with α-cyclodextrin as best drug delivery system. Background:: The screening of new antioxidant compounds and find the good mechanism for binding sites, with correlating between experience and computer theory. Objectives:: The synthesis of seven new antioxidant agents (nitrogen compounds) based on the combination of thiazole, pyridine, triazole and pyrazole moieties. - The studies of their antioxidant activity using DPPH reduction method. - The DFT analysis of the 7 synthesized ligands. - The docking study was also investigated by using the amino acids Ala167 and Arg172. - The better binding affinity with α-cyclodextrin as best drug delivery system. Methods:: Chemistry: synthesis of ligands by condensation reaction - Application: Antioxidant activities using DPPH - Computational studies: using DFT and Docking - Correlation between all these properties. Result:: Chemistry: synthesis of 7 ligands by condensation reaction with 89% yield - Application: Antioxidant activities using DPPH reduction with a good value IC50=13.05 ± 3.73 μg/ml - Computational studies: using DFT (EHOMO and ELUMO) and Docking APX with the amino acids Ala167 and Arg172 compared to the ascorbic acid - Correlation between all these properties : α-cyclodextrin as best drug delivery system (better binding affinity than caffeic acid). Conclusion:: For the drug delivery study, The ACD is best system for all the compounds due to the smallest cavity size which makes the binding affinities favorable and possible to prepare prospective nano-antioxidants.

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