scholarly journals Bacterial cellulose-based biomaterials on third-degree burns in rats

2018 ◽  
Vol 1 (2) ◽  
pp. 31
Author(s):  
Patricia Brassolatti ◽  
Paulo Sérgio Bossini ◽  
Maria Carolina Derencio Oliveira ◽  
Luciana Almeida-Lopes ◽  
Lisinéia Maria Zanardi ◽  
...  
Keyword(s):  
Bacterial Cellulose ◽  
Healing Process ◽  
Treatment Strategies ◽  
High Rate ◽  
Cellulose Membrane ◽  
Post Injury ◽  
Cutaneous Lesions ◽  
Significance Level ◽  
Wound Induction ◽  
Inflammatory Phase

Burns are cutaneous lesions that present  high rate of morbidity and mortality worldwide. In order to innovate the treatment strategies currently applied new biomaterials are being investigated. The aim of the present study was to evaluate the action of bacterial cellulose in both membrane and gel form, in the treatment of third degree burns in rats. For this, 24 Wistar rats were used, divided into three distinct groups. The lesion was performed with the aid of a soldering iron heated at 150 °C pressed on the back of the animal for 10 seconds. Treatment was performed immediately after wound induction, and skin samples were collected on the tenth day post-injury. Statistical analysis was performed using a significance level of 5% (p≤0.05). The histological results show differences in the healing process presented by each group. The group that received bacterial cellulose in the membrane format presented the best results, such as discrete inflammatory infiltrate and better morphological quality of the tissue, characterizing an advanced stage of the healing process, also proven in the collagen quantitative analysis. On the other hand, the group that received the cellulose gel showed characteristics of an inflammatory phase with the presence of evident ulcerations, which corresponds to a delay in the healing process even when compared to CG alone. Thus, it was concluded that before the biomaterials tested cellulose membrane in the format presented more favorable results both in terms of environmental protection as a contribution to an adequate tissue recovery. 

The testing of an artificial modified bacterial cellulose auricle skeleton in an animal model

2019 ◽  
Vol 91 (2) ◽  
pp. 7-11
Author(s):  
Stefan Miśkiewicz ◽  
Bartlomiej Grobelski ◽  
Zbigniew Pasieka ◽  
Marta Miśkiewicz
Keyword(s):  
Animal Model ◽  
Bacterial Cellulose ◽  
Shape Change ◽  
Healing Process ◽  
Surgical Techniques ◽  
Cellulose Membrane ◽  
External Ear ◽  
The Past ◽  
Bacterial Cellulose Membrane

The intention of these study was assess if the modified bacterial cellulose can be used as an artificial auricle skeleton. Introduction: The auricle is one part of the external ear. It consists of skin, cartilage muscles and adipose tissue, wherein the cartilage gives shape to the structure There are several indications for reconstruction, such as congenital anomalies, mechanical injuries and burns, and a range of methods have been proposed for complete reconstruction of the auricle. Matherial and methods: A bacterial cellulose membrane at least 25 mm in thickness was produced in vitro by Actobacter xylinum culture. The entire artificial human auricle skeleton was made to scale to allow its implantation into an animal model Wistar rats Forty rats were divided into four groups of 10 animals. Each group was assigned a different resection time: 14 days, 30 days, 90 days or 720 days. After each resection, an examination of the artificial skeleton and the tissues surrounding it was conducted. The surgical procedure was based on the Nagata technique Results: Resection after 14 , 30 , 90 and 720 days shows progression of healing process and integration of artificial skeleton in to the animal body. There are no signs of shape change and deterioration of the skeleton. Disscusion: Several surgical techniques and biotechnological methods have been developed over the past few years to improve the results of facial reconstruction. Other approaches can be used to create auricle cartilage which are based on scaffolds and chondrocytes. Conclusion: My findings indicate that modified bacterial cellulose can be used to form an effective artificial auricle which appears to maintain its shape and elasticity, with no signs of degradation being observed.

scholarly journals Photobiomodulation with Led 627nm associated to resveratron in cutaneous injuries of rats: a double-blind controlled clinical study.

2020 ◽  
pp. 1-5
Author(s):  
Patricia Pacheco Tyski Suckow ◽  
Ana Carolina Dorigoni Bini ◽  
Paulo Renato de Oliveira ◽  
Larissa Sakis Bernardi ◽  
Danillo Barbosa ◽  
...  
Keyword(s):  
Collagen Fiber ◽  
Healing Process ◽  
Control Group ◽  
Local Circulation ◽  
Double Blind ◽  
Significance Level ◽  
Inflammatory Phase ◽  
Circulation Cell ◽  
Active Release ◽  
Controlled Clinical Study

Introduction: Drugs formulated as Resveratrol, associated with LED 627nm photobiomodulation, can increase the efficacy of active release, increased local circulation, cell proliferation and collagen synthesis, accelerating the healing process. Objective: To analyze the effects of 627nm LED photobiomodulation associated with Resveratrol on the tissue repair of induced wounds in Wistar rats. Methodology: We used 18 animals corresponding to the control groups, group LED 627nm with association to Resveratrol cream (GLed + Resv) 3 and 7 days. Results: Treatment with the use of LED associated with Resveratrol cream provided an efficient healing. In the statistical test, the significance level was observed between the groups of P <0.0156. In the multiple comparison between the pairs the Tukey’s test showed significance between the groups CG vs GLed627nm + Resv 7Day. Conclusion: The GLed + Resv group showed efficient inflammatory phase of healing, promoting a greater activation of fibroblasts and remodeling of the collagen fiber when compared to the control group.

Treatment Strategies Against Diabetic Foot Ulcer: Success so far and Road Ahead

2020 ◽  
Vol 16 ◽  
Author(s):  
Ankit Awasthi ◽  
Sachin Kumar Singh ◽  
Bimlesh Kumar ◽  
Monica Gulati ◽  
Rajesh Kumar ◽  
...  
Keyword(s):  
Lower Limb ◽  
Diabetic Foot ◽  
Treatment Strategies ◽  
Foot Ulcer ◽  
Diabetic Foot Ulcer ◽  
Arterial Disease ◽  
Current Status ◽  
Limb Amputation ◽  
Bibliographic Databases ◽  
Inflammatory Phase

Background: Diabetic foot ulcer (DFU) is one of the leading complications of type-2 diabetes mellitus. It isassociated with neuropathy and peripheral arterial disease of the lower limb in patients with diabetes. Basically, there are four stages of wound healing namely hemostasis phase, inflammatory phase, proliferative phase and maturation phase. In case of DFU, all these stages are disturbed which lead to delay in healing and consequently to lower limb amputation. Traditionally the dosage forms like tablets, creams, ointments, gels and capsules have been used for the treatment of diabetic foot ulcer from many years. Introduction: In this review the global prevalence as well as etiopathogenesis related to diabetic foot ulcer has been discussed. Potential role of various synthetic and herbal drugs as well as their conventional dosage form for the effective management of diabetes foot ulcer has been highlighted. Methods: Structured search of bibliographic databases for previously published peer-reviewed research papers was explored and data was culminated in terms of various approaches that are used for the treatment of diabetic foot ulcer. Results: About 142 papers including both, research and review articles, were included in this review in order to produce a comprehensive as well as readily understandable article. A series of herbal and synthetic drugs have been discussed along with their current status of treatment in terms of dose and mechanism of action. Conclusion: DFU has become one of the most common complications in patients having more than ten years of diabetes. Hence, understanding the root cause and its successful treatment is a big challenge because it depends upon multiple factors such as judicious selection of drug as well as proper control of blood sugar level. Most of the drugs that have been used so far either belong to the category of antibiotics, antihyperglycaemics or, they have been repositioned. Moreover, in clinical practice, much focus has been given towards dressings that have been used to cover the ulcer. The complete treatment of DFU is still a farfetched dream to be achieved and it is expected that a combination therapy of herbal and synthetic drug with multiple treatment pathway could be able to overcome the disease.

scholarly journals AB1267 ONLINE CASE-BASED EDUCATION SIGNIFICANTLY IMPROVED RHEUMATOLOGISTS’ KNOWLEDGE AND CONFIDENCE IN MANAGING PATIENTS WITH RA

2020 ◽  
Vol 79 (Suppl 1) ◽  
pp. 1925.2-1925
Author(s):  
E. Bell ◽  
A. Sendaydiego ◽  
P. C. Taylor
Keyword(s):  
Rapid Evolution ◽  
Safety Data ◽  
Treatment Strategies ◽  
Further Education ◽  
Learning Activity ◽  
Tnf Inhibitor ◽  
Jak Inhibitors ◽  
Significance Level ◽  
Average Percentage ◽  
Post Assessment

Background:With the rapid evolution in treatment strategies and the increasing range of available therapeutics for rheumatoid arthritis (RA), keeping pace with advances can be a challenge for busy physicians.Objectives:We assessed whether online CME can improve rheumatologists’ knowledge of RA management focusing on the assessment and monitoring of RA, the selection of appropriate treatments and the clinical efficacy and safety data for JAK inhibitors.Methods:Rheumatologists participated in a text-based activity featuring two patient cases with questions that “tested” knowledge and discussion of the main “teaching” points. Educational effect was assessed using a repeated-pair design, pre-/post-assessment. A Chi-square test of independence determined if a statistically significant improvement (5% significance level,P<.05) existed in the number of correct responses between the pretest and posttest scores. Cramer’s V estimated the effect size of the education. The activity launched on 15 December 2018 with data collection through 27 February 2019.Results:•Significant improvement in average percentage of correct responses, rising from 47% at baseline to 92% post-activity (P<.001) and extensive educational impact (Cramer’s V=0.496)•Significant increase in percentage of rheumatologists (n=111) answering all 3 questions correctly (9% at baseline rising to 78% post assessment)•Significant improvements in knowledge of EULAR/ACR assessment criteria (86% improvement,P<.001), EULAR treatment recommendations for a patient failing on MTX and a TNF inhibitor (100% improvement,P<.001), and the VTE risk associated with having RA or receiving RA treatments (108% improvement,P<.001)•46% of rheumatologists reported greater confidence in their ability to appropriately incorporate JAK inhibitors into the treatment of patients with RA (average confidence shift 20%)Conclusion:Overall, this learning activity was highly successful in improving rheumatologists’knowledge and confidence in managing patients with RA, particularly with regard to the appropriate use of JAK inhibitors in patients for whom such treatment is suitable. The extensive impact of this interactive ‘test then teach’ activity is likely to directly translate into patient benefit. Further education on this topic would be useful to enhance and reinforce this knowledge and to support physician confidence in the use of JAK inhibitors in clinical practice.References:[1]https://www.medscape.org/viewarticle/906202Disclosure of Interests:Elaine Bell: None declared, Anne Sendaydiego: None declared, Peter C. Taylor Grant/research support from: Celgene, Eli Lilly and Company, Galapagos, and Gilead, Consultant of: AbbVie, Biogen, Eli Lilly and Company, Fresenius, Galapagos, Gilead, GlaxoSmithKline, Janssen, Nordic Pharma, Pfizer Roche, and UCB

scholarly journals Bacterial Cellulose Membrane Containing Epilobium angustifolium L. Extract as a Promising Material for the Topical Delivery of Antioxidants to the Skin

2021 ◽  
Vol 22 (12) ◽  
pp. 6269
Author(s):  
Anna Nowak ◽  
Paula Ossowicz-Rupniewska ◽  
Rafał Rakoczy ◽  
Maciej Konopacki ◽  
Magdalena Perużyńska ◽  
...  
Keyword(s):  
Bacterial Cellulose ◽  
Topical Application ◽  
Phenolic Acid ◽  
Antioxidant Properties ◽  
Ethanolic Extract ◽  
Cellulose Membrane ◽  
Hydroxybenzoic Acid ◽  
Epilobium Angustifolium ◽  
Bacterial Cellulose Membrane

Bacterial cellulose membranes (BCs) are becoming useful as a drug delivery system to the skin. However, there are very few reports on their application of plant substances to the skin. Komagataeibacter xylinus was used for the production of bacterial cellulose (BC). The BC containing 5% and 10% ethanolic extract of Epilobium angustifolium (FEE) (BC-5%FEE and BC-10%FEE, respectively) were prepared. Their mechanical, structural, and antioxidant properties, as well as phenolic acid content, were evaluated. The bioavailability of BC-FESs using mouse L929 fibroblasts as model cells was tested. Moreover, in vitro penetration through the pigskin of the selected phenolic acids contained in FEE and their accumulation in the skin after topical application of BC-FEEs was examined. The BC-FEEs were characterized by antioxidant activity. The BC-5% FEE showed relatively low toxicity to healthy mouse fibroblasts. Gallic acid (GA), chlorogenic acid (ChA), 3,4-dihydroxybenzoic acid (3,4-DHB), 4-hydroxybenzoic acid (4-HB), 3-hydroxybenzoic acid (3-HB), and caffeic acid (CA) found in FEE were also identified in the membranes. After topical application of the membranes to the pigskin penetration of some phenolic acid and other antioxidants through the skin as well as their accumulation in the skin was observed. The bacterial cellulose membrane loaded by plant extract may be an interesting solution for topical antioxidant delivery to the skin.

Cholangiocarcinoma therapeutics: an update

2021 ◽  
Vol 21 ◽  
Author(s):  
Mai Ly Thi Nguyen ◽  
Nguyen Linh Toan ◽  
Maria Bozko ◽  
Khac Cuong Bui ◽  
Przemyslaw Bozko
Keyword(s):  
Treatment Outcome ◽  
Targeted Therapies ◽  
Treatment Strategies ◽  
Favourable Outcome ◽  
High Rate ◽  
Postoperative Treatment ◽  
Clinical Indication ◽  
Tumour Resection ◽  
First Line ◽  
Treatment Methods

Background: Background: Cholangiocarcinoma (CCA) is the second most common hepatobiliary cancer and associated with poor prognosis. Only one-third of CCA cases are diagnosed at operable stages. However, a high rate of relapse has been observed post-operatively. Besides screening for operable individuals, efficacious therapeutic for recurrent and advanced CCA is urgently needed. Treatment outcome of available therapeutics is important to clarify clinical indication and facilitate development of treatment strategies. Objective: This review aims to compare the treatment outcome of different therapeutics based on both overall survival and progression-free survival. Methods: Over one hundred peer-reviewed articles were examined. We compared the treatment outcome between different treatment methods, including tumour resection with or without postoperative systematic therapy, chemotherapies including FOFLOX and targeted therapies, such as IDH1, K-RAS and FGFR inhibitors. Notably, the scientific basis and outcome of available treatment methods werecompared with the standard first-line therapy. Results: CCAs at early stages should firstly undergo tumour resection surgery, followed by postoperative treatment with Capecitabine. Chemotherapy can be considered as a preoperative option for unresectable CCAs. Inoperable CCAs with genetic aberrances like FGFR alterations, IDH1 and K-RAS mutations should be considered with targeted therapies. Fluoropyrimidine prodrug (S-1)/Gemcitabine/Cisplatin and nab-Paclitaxel/Gemcitabine/Cisplatin show favourable outcome which hints on the triplet regimen to be superior to Gemcitabine/Cisplatinon CCA. Thetriplet chemotherapeutic should be tested further compared toGemcitabine/Cisplatin among CCAs without genetic alterations.Gemcitabine plus S-1 was recently suggested as the convenient and equivalent standard first line for advanced/recurrent biliary tract cancer. Conclusions: Thisreview provides a comparative outcomebetween novel targeted therapies and currently available therapeutics.

Caustic and Toxic Ingestions

2017 ◽  
Author(s):  
CDR Thomas Q Gallagher ◽  
CDR Robert L Ricca
Keyword(s):  
Surgical Management ◽  
Management Strategies ◽  
Treatment Strategies ◽  
Tracheobronchial Tree ◽  
High Rate ◽  
Health Concern ◽  
Endoscopic Management ◽  
Caustic Ingestion ◽  
Stricture Formation ◽  
Accidental Ingestion

Ingestion of caustic substances remains a potentially fatal public health concern with extensive morbidity and the possibility of long-term sequelae. The management strategies of these complex injuries continue to be extensively studied in the literature. Areas of interest include the most efficacious treatment of caustic esophageal stricture to preserve the native esophagus, use of steroids, and use of esophageal stents. Prevention of accidental ingestion through strategies to limit the availability of caustic substances is a key factor in reducing the incidence of injury, but there continues to be a high rate of accidental ingestion in developing countries with less rigorous manufacturing standards. Initial evaluation includes endoscopic evaluation of the esophagus and tracheobronchial tree. Optimal treatment strategies, including the use of proton pump inhibitors to reduce gastroesophageal reflux, steroid use to prevent stricture formation, and use of stents for management of strictures, continue to be debated. Initial surgical management includes esophagectomy for full-thickness injury with abdominal exploration. Multiple surgical options exist for both restoration of gastrointestinal continuity after esophagectomy and the management of strictures refractory to medical management, including reverse gastric tube, colonic interposition, and gastric advancement. Numerous small studies have evaluated the efficacy of these interventions, but there continues to be a need for larger prospective studies to develop a worldwide consensus opinion on best practices. We provide a review of the recent literature and practice recommendations for the management of injuries due to caustic ingestion. Key words: caustic ingestion, endoscopic management, stricture, surgical management 

scholarly journals Production of vegetable oil blends and structured lipids and their effect on wound healing

2015 ◽  
Vol 51 (2) ◽  
pp. 415-427 ◽  
Author(s):  
Juliana Neves Rodrigues Ract ◽  
Fabiana Andreia Schäfer De Martini Soares ◽  
Hosana Gomes Rodrigues ◽  
José Ricardo Bortolon ◽  
Gilson Masahiro Murata ◽  
...  
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Structured Lipids ◽  
Physiological Saline ◽  
Structured Lipid ◽  
Control Group ◽  
Wound Healing Process ◽  
Oil Blends ◽  
Inflammatory Phase ◽  
Canola Oils

<p>Two oil blends (sunflower/canola oils 85/15 (BL1) and canola/linseed oils 70/30 (BL2)), were prepared and enzymatically interesterified to be applied to surgically-induced wounds in rats. Following surgery, the animals were submitted to the Treatment with Physiological Saline (TPS) (control group), Blends (TBL), and Structured Lipids (TSL). The control group (TPS) received physiological saline solution for 15 days. In TBL, BL1 was administered during the inflammation phase (days 0-3) and BL2 in the tissue formation and remodeling phase (days 4-15). In TSL, Structured Lipid 1 (SL1) and Structured Lipid 2 (SL2) were used instead of BL1 and BL2, respectively. The aim of this study was to compare wound closure evolution among rats treated with the blends or structured lipids versus control rats treated with physiological saline. The wound healing process was evaluated by measuring the wound areas along the treatments and the concentrations of cytokines. An increase in the areas of wounds treated with the blends and structured lipids in the inflammatory phase was observed, followed by a steeper closure curve compared to wounds treated with physiological saline. The changes observed during the inflammatory phase suggest a potential therapeutic application in cutaneous wound healing which should be further investigated.</p>

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