PEMANFAATAN AIR KELAPA UNTUK MENINGKATKAN PERTUMBUHAN AKAR STEK TUNAS AKSILAR Andrographis paniculata Nees

The research has been conducted to find out the best concentration of coconut water in the induction of roots of cuttings axillary buds Andrographis paniculata Nees. This research was conducted in Plant Physilogy of Division, Department of Biology, Faculty of Math and Natural Science, AndalasUniversity, for 12 weeks. The research used Completely Randomized Design. The material used was axillary buds A. paniculata with nodal number 3. treated for 30 minutes in coconut water 10, 15 and 20% and aquadest as a controle each with 6 replications. Observations were done on live percentage, root length, root numerous and cuttings height after 12 weeks planting. The results showed that the concentration 10 to 15% of coconut water was able with significant to induce the root growth of cutting axillary buds A. Paniculat. The concentration 15% of coconut water was the best in inducing cutting height growth, each with an average root length is 8.2 cm and the average number of roots that formed is 16, 0 and the average cutting height is 22.7 cm. There was an increase of average of cuttings height growth by 86% which was supported by the average increase of root length and root number of 105.0% and 280.9% on the occasion of cuttings of axillary buds in coconut water concentration 15%.

Download Full-text

Subsitusi Fitohormon Dengan Air Kelapa (Cocos nucifera L.) pada Medium Vacin and Went Terhadap Pertumbuhan Eksplan Anggrek Dendrobium sp Secara in Vitro

Jurnal SAINS ALAMI (Known Nature) ◽

10.33474/j.sa.v3i2.7222 ◽

2021 ◽

Vol 3 (2) ◽

Author(s):

Wulan Dari Neng Gumiwang ◽

Tintrim Rahayu ◽

Ari Hayati

Keyword(s):

Root Length ◽

Cocos Nucifera ◽

Water Concentration ◽

Coconut Water ◽

Culture Bottle ◽

Randomized Design ◽

Number Of Leaves ◽

Completely Randomized Design ◽

Cocos Nucifera L

The purpose of this research is to determine the concentration of young coconut water that is appropriate for the growth of orchid plantlets (Dendrobium sp.) In vitro. This study used an experimental method, descriptive data analysis to compare several different concentrations of coconut water. The design of this study uses a completely randomized design (CRD). The treatments consist of 0% coconut water concentration (as a control), 15%, 30% and 60%. Each concentration was carried out 5 replications and each repetition consisted of 5 Dendrobium sp plantlets in each culture bottle conducted for 40 HST, for observing the root length carried out for 50 HST. The highest number of shoots and leaves were produced at the same concentration, namely 150 ml / L coconut water treatment (15% concentration) with an average of 2.8 shoots and the average number of leaves 10.8 leaves. The average number of roots and the longest root length was produced at a concentration of 600 ml / L coconut water (60% concentration) with an average of 6 roots, and the longest root length was 0.5 cm.Keywords: Young coconut water, (Cocos nucifera L.), Dendrobium sp., in vitro, growth.ABSTRAKTujuan penelitian ini ialah menentukan konsentrasi air kelapa muda yang tepat untuk pertumbuhan planlet anggrek (Dendrobium sp.) secara in vitro. Penelitian ini menggunakan metode eksperimen, analisis data secara deskriptif untuk membandingan beberapa konsentrasi air kelapa yang berbeda. Rancangan penelitian ini menggunakan Rancangan Acak Lengkap (RAL). Perlakukan terdiri dari konsentrasi air kelapa 0 % (sebagai kontrol), 15% , 30% dan 60%. Masing-masing konsentrasi dilakukan 5 kali ulangan dan setiap ulangan terdiri dari 5 planlet Dendrobium sp dalam setiap botol kultur yang dilakukan selama 40 HST, untuk pengamatan panjang akar dilakukan selama 50 HST. Jumlah tunas dan jumlah daun terbanyak dihasilkan pada konsentrasi yang sama, yaitu perlakuan air kelapa 150 ml/L (konsentrasi 15%) dengan rata-rata jumlah tunas terbanyak 2,8 tunas dan rata-rata jumlah daun terbanyak 10,8 helai daun. Rata-rata jumlah akar terbanyak dan panjang akar terpanjang dihasilkan pada konsentrasi air kelapa 600 ml/L (Konsentrasi 60%) dengan rata-rata jumlah akar terbanyak sebanyak 6 akar, dan rata-rata panjang akar terpanjang 0,5 cm.Kata kunci : Air kelapa Muda (Cocos nucifera L.), Dendrobium sp., in vitro, pertumbuhan

Download Full-text

Pertumbuhan Dan Perkembangan Anggrek Dendrobium anosmum Pada Media Kultur In Vitro Dengan Beberapa Konsentrasi Air Kelapa

Agrologia ◽

10.30598/a.v1i1.293 ◽

2018 ◽

Vol 1 (1) ◽

Author(s):

S. Tuhuteru ◽

Meity L Hehanussa ◽

Simon H.T Raharjo

Keyword(s):

Culture Medium ◽

Culture Media ◽

Water Concentration ◽

Medium Composition ◽

Coconut Water ◽

Orchid Species ◽

Randomized Design ◽

Wet Weight ◽

Completely Randomized Design

Dendrobium anosmum is one of natural orchids in Indonesia. Optimization of medium composition for orchid propagation through in vitro culture is necessary to enhance propagule multiplication capabilities and quality. This study was aimed to study the influence of concentration of coconut water in culture medium on in vitro growth and development of D. anosmum orchid species and to determine the optimal coconut water concentration in culture media. The experiment were arranged in a Completely Randomized Design with four treatments and eight replications. The treatments consisted of the addition of coconut water with concentrations: 0 ml•l -1 (control), 50 ml•l-1, 100 ml•l-1 and 150 ml•l-1. The results showed that addition of coconut water in culture medium gave different effect on shoot growth and multiplication of D. anosmum orchids. Coconut water concentration of 100 ml•l-1 was the best concentration for growth and multiplication of D. anosmum orchids, based on both shoots and roots growth, plantlet height and wet weight.

Download Full-text

THE ADDITION OF COCONUT WATER AND THIAMINE TOWARDS CHRYSSANTHENUM MICRO CUTTINGS (Dendranthema grandiflora Tzvelev) IN VITRO

Agrivet ◽

10.31315/agrivet.v27i1.4694 ◽

2021 ◽

Vol 27 (1) ◽

pp. 39

Author(s):

Salma Nabila ◽

Endah Budi Irawati ◽

Rina Srilestari

Keyword(s):

Water Concentration ◽

Coconut Water ◽

Fresh Weight ◽

Randomized Design ◽

Two Factors ◽

Completely Randomized Design ◽

Range Test ◽

Growing Shoot

Chryssanthenum is ornamental plant with variety of shape and color which are unique and appealing. So that, it is in great demand in the community. The production of Chrissanthenum conventionally hampered by availability and quality of seeds. Thus, it needs research through tissue culture. The aim of this research is to know interaction between coconut water and thiamine and to determine the best coconut water and thiamine concentration toward Chryssanthenum micro cuttings. This research used laboratory experimental method by using completely randomized design with two factor. The 1st factor was coconut water concentration consisted of three level which were 5%, 10% and 15%. The 2nd factor was thiamine concentration consisted of three level which were 1mg/L, 2 mg/L and 3 mg/L. From the two factors, those were found that, there were nine combination of treatments and repeated 3 times. The variety of data was analyzed by using Analysis of Variance (ANOVA) with level of α=5%, and continued by examining Duncan’s Multiple Range Test (DMRT) with level of α=5%. The result indicated that the interaction of coconut water concentration was 5% and thiamine was 1 mg/L on the parameters when growing shoot. There was also interaction on coconut water concentration which was 10 % and thiamine was 1 mg/L on the parameters in the number of shoots. interaction of coconut water combination was 15% and thiamine was 2 mg/L on fresh weight. The addition of 10% coconut water and 1 mg/L thiamine showed the best result on shoot length.

Download Full-text

Application of Young Coconut Water and “Kascing” Fertilizer to Red Palm Seed Germination

Jurnal ILMU DASAR ◽

10.19184/jid.v18i1.2115 ◽

2017 ◽

Vol 18 (1) ◽

pp. 17

Author(s):

Fatimatuz Zuhro ◽

Hasni Ummul Hasanah ◽

Sukadi Sukadi

Keyword(s):

Seed Germination ◽

Germination Rate ◽

Water Concentration ◽

Germination Percentage ◽

Coconut Water ◽

Randomized Design ◽

Completely Randomized Design

Redpalm isornamentplantwhichpotentiallytobecultured.Thescarcityofredpalmmadeithashighmarket value and need to be preserved. However, the germination growth of red palm is slower than other kinds of palm, so the best way to germinate the red palm seed is needed. The aim of this research is to determine the effect of apliccation some young coconut water concentration and kascing fertilizer to red palm seed germination. The research used factorial completely randomized design with 5 replication, the first factor is young coconut water concentration (0%, 25%, 50%, 75%, and 100%) and the second factor is germination media (steril sand and kascing fertilizer). Variables measured were live germination percentage, speed germination rate, radicula length, and plumula height. Results of this study showed that the best germination media is steril sand and 0% concentration of young coconut water.

Download Full-text

PENGARUH PENAMBAHAN EKSTRAK JAGUNG MANIS DAN AIR KELAPA PADA MEDIA MS SECARA IN VITRO TERHADAP INDUKSI TUNAS ADVENTIF BAWANG PUTIH (Allium sativum L.)

Jurnal Ilmu-Ilmu Pertanian Indonesia ◽

10.31186/jipi.23.2.92-97 ◽

2021 ◽

Vol 23 (2) ◽

pp. 92-97

Author(s):

Marisa Gracia Bakara ◽

Makhziah Makhziah ◽

Guniarti Guniarti

Keyword(s):

Allium Sativum ◽

Sweet Corn ◽

Water Concentration ◽

Coconut Water ◽

Time Treatment ◽

Concentration Time ◽

Randomized Design ◽

Completely Randomized Design ◽

Four Levels

[SWEET CORN EXTRACT AND COCONUT WATER EFFECT TO INDUCE GARLIC (Allium sativum L.) ADVENTIST SHOOTS BY IN-VITRO CULTURE]. Garlic (Allium sativum L.) is one of the horticultural plants used daily for various uses. This study aims to determine the best extract concentration of sweet corn and coconut water and its interaction for induction garlic Adventist shoots (Allium sativum L.) var. Lumbu Hijau. The study was conducted at the Biotechnology Laboratory, Agriculture Faculty, UPN ”Veteran” East Java, in February – April 2021. The experimental design used was a factorial completely randomized design (CRD). The first factor is four levels of extract of sweet corn concentration (0, 50, 100, and 150 g/L), while the second factor is four levels of coconut water concentration (0, 50, 100, and 150 mL/L). The results showed that the extract of sweet corn concentration-time treatment increased the growing adventist shoots percentage, adventist shoots number, and adventist shoots size. The section of sweet corn concentration of 150 g/L increased the rate of growing adventist shoots, adventist shoots number, and adventist shoots size. The treatment of coconut water concentration and its interaction with the extract of sweet corn concentration is still not able for induction garlic adventist shoots (Allium sativum L.) of the var. Lumbu Hijau.

Download Full-text

Kemampuan berakar setek pucuk dari beberapa tanaman induk Pinus merkusii kandidat bocor getah

Jurnal Pemuliaan Tanaman Hutan ◽

10.20886/jpth.2020.13.2.71-83 ◽

1996 ◽

Vol 13 (2) ◽

pp. 71-83

Author(s):

Gunawan Nugrahanto ◽

◽

Mohammad Na'iem ◽

Sapto Indrioko ◽

Eny Faridah ◽

...

Keyword(s):

Height Growth ◽

P Value ◽

Progeny Trial ◽

Primary Roots ◽

Randomized Design ◽

Completely Randomized Design ◽

Almost All ◽

Cutting Height ◽

Secondary Roots ◽

Pinus Merkusii

This study aims to examine the rooting ability of 24 clones of Pinus merkusii with high resin yield. The explants were propagated by shoot cutting that produced callus. The variables of root cuttings ability were root percentage, cutting height growth, number and length of primary roots, number and length of secondary roots, root volume, root biomass, and shoot-root ratio. The study was carried out in a Completely Randomized Design with treatments of 14 clones from hedge orchard (called as pkp clone hereafter) and 10 clones from a progeny trial (called as ppt clone hereafter) with 4 treeplots and 4 replications. The results showed that clones' origin had caused differences in root percentage, length of primary roots, number and length of secondary roots, and shoot-root ratio (P value <0.05). Clones originated from hedge orchard were always significantly better. Almost all clones from hedge orchard have a good rooted ability (>70%). Meanwhile, the clones from the progeny trial showed better height growth.

Download Full-text

Pengaruh Pemberian Air Kelapa dan Tipe Sambungan terhadap Keberhasilan Sambung Pucuk Durian (Durio zibethinus M.)

Agrosains: Jurnal Penelitian Agronomi ◽

10.20961/agsjpa.v15i2.19000 ◽

2013 ◽

Vol 15 (2) ◽

pp. 46

Author(s):

Ika Trisna Yanti ◽

Sulandjari Sulandjari ◽

Endang Yuniastuti

Keyword(s):

Plant Growth ◽

Water Concentration ◽

Complex Compounds ◽

Coconut Water ◽

Water Complex ◽

Durio Zibethinus ◽

Randomized Design ◽

Total Treatment ◽

Completely Randomized Design ◽

Germinating Seeds

<p>Durian is a tropical native fruit from Indonesia with a distinctive flavor. Durian can be propagated durian generatively and vegetatively. Generative propagation of durian can be done by germinating seeds, which is vegetative propagation can be done by top grafting. Top grafting can be accelerated by the addition of plant growth regulators. Growth regulating substances in coconut water complex compounds are useful in differentiating cells. This study aimed to determine the effect of various concentrations of coconut water and the best type of grafting for the success of durian tops grafting. In this study was conducted in horticultural breeding centers Ranukitri Mojogedang Karanganyar April to September 2012. This research used Completely Randomized Design (CRD), which consisted of 2 types of treatment that top grafting, namely: cleft grafting, slash grafting, side grafting and coconut water concentration with 5 levels: 0%, 25%, 50%, 75%, 100% with 5 replications, so that the total treatment was 75. The results showed that the type of cleft grafting was the best grafting type to be used for the success of top grafting compared with other grafting types. Treating coconut water with a concentration of 25% was able to optimize the growth of durian top grafting than other concentrations.</p>

Download Full-text

Influence of NAA and coconut water with variation of soaking duration on the growth of yellow bamboo branch cutting

Nusantara Bioscience ◽

10.13057/nusbiosci/n100308 ◽

2018 ◽

Vol 10 (3) ◽

pp. 178-182

Author(s):

TIA SETIAWATI ◽

AGINTA PUTRI REHULINA KELIAT ◽

RULY BUDIONO ◽

RUHYAT PARTASASMITA ◽

JOHAN ISKANDAR

Keyword(s):

Leaf Area ◽

Root Length ◽

Raw Materials ◽

Shoot Length ◽

Coconut Water ◽

Root Number ◽

Randomized Design ◽

Shoot Number ◽

Bambusa Vulgaris ◽

Branch Cutting

Setiawati T, Keliat APR, Budiono R, Partasasmita R, Iskandar J. 2018. Influence of NAA and coconut water with variation of soaking duration on the growth of yellow bamboo branch cutting. Nusantara Bioscience 10: 178-182. Yellow bamboo (Bambusa vulgaris Schrad Ex. var. Striata) is one kind of bamboo used for industrial and household raw materials. Yellow bamboo propagation with branch cuttings has relatively low percentage of growth. To increase the growing success of yellow bamboo branch cuttings can be used growth regulators substance, such as NAA and coconut water. This study aimed to obtain the best combination of NAA and coconut water with the soaking duration in the growth of yellow bamboo branch cuttings. The study was conducted using Completely Randomized Design (CRD) of 6 × 3 factorial arrangement with five replications. The first factor was the combination of NAA and coconut water (CW), which consisted of six levels, namely 100% CW, 20% NAA + 80% CW, 40% NAA + 60% CW, 60% NAA + 40% CW, 80% NAA + 20% CW and 100% NAA. The second factor was the soaking duration which consists of three levels, namely 12 h, 24 h, and 36 h. Parameters observed included shoot emerging time, shoot number, shoot length, leaf area, root number and root length. The data obtained were analyzed using the Analysis of Variance (ANOVA) and Duncan's Multiple Range Test (DMRT) α=5%. The results showed that the combination of 80% NAA + 20% CW gave the best yellow bamboo branch cuttings growth with an average shoot length of 1.44 cm, leaf area 41.29 cm2, root number 42.87 and root length 23.70 cm. The interaction of 80% NAA + 20% CW with soaking duration of 36 h resulted in average the fastest shoot emerging time of 2.02 days after planting.

Download Full-text

Kemampuan berakar setek pucuk dari beberapa tanaman induk Pinus merkusii kandidat bocor getah

Jurnal Pemuliaan Tanaman Hutan ◽

10.20886/jpth.2019.13.2.71-83 ◽

1996 ◽

Vol 13 (2) ◽

pp. 71-83

Author(s):

Gunawan Nugrahanto ◽

◽

Mohammad Na'iem ◽

Sapto Indrioko ◽

Eny Faridah ◽

...

Keyword(s):

Height Growth ◽

P Value ◽

Progeny Trial ◽

Primary Roots ◽

Randomized Design ◽

Completely Randomized Design ◽

Almost All ◽

Cutting Height ◽

Secondary Roots ◽

Pinus Merkusii

Download Full-text

THE SOURCES OF AUXIN HORMONE TO GROWTH OF SHOOT PINEAPPLE STEM CUTTING (Ananascomosus.L. Merr.)

Journal of Academic Research and Sciences (JARES) ◽

10.30957/jares.v1i1.41 ◽

2016 ◽

Vol 1 (1) ◽

pp. 45-52

Author(s):

Palupi Puspitorini

Keyword(s):

Water Concentration ◽

Urine Concentration ◽

Experimental Results ◽

Stem Cuttings ◽

Randomized Design ◽

Natural Hormone ◽

Cow Urine ◽

Urine Concentrations ◽

Completely Randomized Design ◽

Better Than

The aim of this study was to select the best sources of auxin of which it can stimulate the growth of shoots Pineapple plant cuttings. This research is compiled in a completely randomized design (CRD) with 4 treatments and 6 replications. The Data were statistically Analyzed by the DMRT. Level of treatment given proves that no treatment 0%, cow urine concentration of 25%, young coconut water concentration of 25% and Rootone F 100 mg / cuttings. The results showed that cow urine concentrations of 25% and Rootone F 100 mg give the best results in stimulating the growth of shoots pineapple stem cuttings. Experimental results concluded that the effect of this natural hormone were better than the shoots without given hormone.

Download Full-text