scholarly journals SUPLEMENTASI BAGASE DENGAN ENZIM SELULASE DAN PENGARUHNYA TERHADAP KECERNAAN BAHAN KERING, BAHAN ORGANIK DAN SERAT KASAR SECARA IN VITRO

2015 ◽  
Vol 1 (1) ◽  
pp. 84
Author(s):  
Yetti Marlida ◽  
Mardiati Zain
Keyword(s):  
Organic Matter ◽  
Dry Matter ◽  
Animal Feed ◽  
In Vitro Digestion ◽  
Industrial Biotechnology ◽  
Liquid Form ◽  
Industrial Residues ◽  
Cellulase Enzyme ◽  
Key Words Cellulase

 ABSTRACTSupplementation of cellulase enzyme to agro-industrial residues can increase the nutritional value of the product. Advances in industrial biotechnology offer potential opportunities for economic utilization of agro-industrial residues such as palm pres fiber, rice straw, corn straw and bagase for animal feed. Bagase is a complex material, is the major by product of the sugar cane industry.  This experiment examined the effect of applying a cellulase enzyme (Siqma & Co) on the digestibility of bagase in vitro.  The enzyme was applied in liquid form at  concentrations 1.0; 1.5 and 2.0 (g/100 g DM) to bagase. The bagase was incubated with enzyme for 48 h before in vitro digestion. The digestibility of dry matter, organic matter and fiber  in vitro was detected. The results showed that digestibility of dry matter, organic matter and fiber were significantly different (P<0.01) across treatments. The increased of the cellulase enzyme supplemented to bagase can increased the nutritive values of the product and directly digestibility of dry matter, organic matter and fiber higher.  Results from this study indicated that direct application of enzymes to bagase was capable to improving  digestibility in-vitro. Key words :  cellulase enzyme, bagase, digestibility, in vitro

scholarly journals Soaking in Seawater of Rice Straw Increases In vitro Dry Matter and Organic Matter Digestibility

2017 ◽  
Vol 2 (2) ◽  
pp. 1-6
Author(s):  
H. Husnaeni ◽  
M. Arifin Amril ◽  
Sjamsuddin Rasjid
Keyword(s):  
Organic Matter ◽  
Rice Straw ◽  
Dry Matter ◽  
Nutritional Value ◽  
Sea Water ◽  
Animal Feed ◽  
Dry Matter Digestibility ◽  
Length Relationship ◽  
Organic Matter Digestibility

This research aims to improve the digestibility and nutritional value of rice straw as a ruminant animal feed and determine the effect of long soaking in sea water of rice straw on digestibility in vitro dry matter and organic matter. The used materials in this study are a bales machine, weighing scales, rapiah rope, poly bags, rice straw varieties Ciliwung obtained from rice fields in Bantimurung, sea water with 2.71% saline, as well as tools and chemicals for analysis of in vitro dry matter and organic matter digestibility with pepsin, calculus method. This study is done based on Randomized Complete  Design (RCD) with 5 treatments and 4 replications. Variance based on the long soaking treatment gives the nature of the response curve linear on the digestibility of dry matter and also to organic matter digestibility. The magnitude of the correlation length relationship soaking rice straw and with sea water for in vitro dry matter and organic matter digestibility that is 96.5% and 96.8% respectively. Based on the results of research and discussion can be concluded that rice straw is soaked with sea water 3-12 days producing dry matter digestibility and organic matter are higher than the rice straw that is not soaked with sea water. Soaking of the sea water for 3-12 days increase the digestibility of dry matter and organic matter.

Prediction of in vivo dry matter, organic matter, and energy digestibilities of silage in in vitro digestion techniques

1971 ◽  
Vol 22 (5) ◽  
pp. 787 ◽  
Author(s):  
GD Brown ◽  
JC Radcliffe
Keyword(s):  
Organic Matter ◽  
Volatile Compounds ◽  
Dry Matter ◽  
In Vitro Digestion ◽  
Digestion Time ◽  
Dry Matter Digestibility ◽  
Highly Correlated ◽  
Ad Libitum Intake

Twenty experimental silages were made from seven pasture species at different stages of maturity. In vivo dry matter, organic matter, and energy digestibilities of the silages were determined at restricted and ad libitum intake levels on standardized pairs of Merino wethers. In vitro dry matter digestibility determinations were made on ground frozen silage and ground oven-dried silage with and without a correction for the loss of volatile compounds during drying. Corrected in vitro dry matter digestibilities of ground oven-dried silage for a 48 hr rumen liquor digestion time gave the most accurate prediction of in vivo dry matter (r = 0.88), organic matter (r = 0.86), and energy (r = 0.74) digestibilities for restricted intake levels. In vitro digestibilities of ground frozen silage more accurately predicted in vivo digestibilities than did in vitro digestibilities of ground oven-dried silage without the correction for volatile losses. In vivo dry matter and organic matter digestibilities were highly correlated (v = 0.97), but both were poorly correlated with in vivo energy digestibilities. In vitro digestion of ground oven-dried silage with a correction for the volatile compounds lost during drying is recommended as a suitable routine determination for predicting the in vivo digestibility of silage.

scholarly journals Note on in vitro digestion of avocado products for pigs

2021 ◽  
Vol 51 (1) ◽  
Author(s):  
J. Ly ◽  
P. Fránquez ◽  
G. Rodríguez ◽  
C. Lemus ◽  
I.A. Dominguez ◽  
...  
Keyword(s):  
Organic Matter ◽  
Dry Matter ◽  
Nutritional Value ◽  
In Vitro Digestion ◽  
In Vitro Digestibility ◽  
Optimal Production ◽  
Additional Protein

In vitro (pepsin/pancreatin) digestibility coefficients of pulp, seed, and peel of avocados of the Creole and Hass varieties were studied according to a 2 x 3 factorial arrangement in which the factors were the variety and parts of the fruit. Creole avocado were heavier in natura than Hass exemplars (310.7 g and 156.8 g), whereas Hass had a greater proportion of pulp than the Creole fruits (76.0% and 64.6%). No significant interactions were observed. In vitro digestibility of dry matter (DM), organic matter (OM), and nitrogen (N) were higher for the Creole variety compared with the Hass. In vitro digestibility of N was similar in the pulp and seeds, and higher than in the peels (731, 773, and 550 g/kg-1, respectively). Use of the entire avocado as pig feedstuff would have a lower nutritional value compared with feeding just avocado pulp. Supplementation of a diet based on avocado with additional protein may be needed for optimal production.

PENGARUH PERLAKUAN ALKALI PADA LIMBAH KULIT KOPI DAN PENINGKATAN KECERNAANNYA DENGAN TEKNIK IN VITRO

2018 ◽  
Vol 5 (2) ◽  
Author(s):  
A. Fariani., dkk
Keyword(s):  
Organic Matter ◽  
Dry Matter ◽  
Animal Feed ◽  
Alkali Treatment ◽  
In Vitro Digestibility ◽  
Coffee Pulp ◽  
Dry Matter Digestibility ◽  
Tannin Concentration ◽  
Organic Matter Digestibility

This research was done to improve coffee pulp digestibility with alkali treatment and adding organic acid by in vitro. This research was done in two stage, fi rst stage was soaking in alkali fl uid and second stage was in vitro digestibility analysis, in Animal Feed and Nutrition Laboratory, Faculty of Agriculture, Sriwijaya University. Completely Randomized Design (CRD) used in 4 reatments and feed treatment was replicated 3 times. The treatments were: P0 (control), P1 (2 day soaking), P2 (3 day soaking) and P3 (4 day soaking). Observe parameters were tannin concentration, dry matter digestibility, organic matter digestibility, concentration of N-NH3 and (pH). The result showed that the treatment had nosignifi cant diference (P0.01) on tannin concentration. However, it was signifi cantly difference on dry matter digestibility, organic matter digestibility, concentration of N-NH3, and (pH).Key Words: Coffee pulp, alkali treatment, digestibility, in vitro techniques

INTERACTION BETWEEN INOCULUM DONOR DIET AND SUBSTRATE IN IN VITRO RUMINANT DIGESTION STUDIES

1966 ◽  
Vol 46 (2) ◽  
pp. 91-95 ◽  
Author(s):  
J. E. Knipfel ◽  
J. E. Troelsen
Keyword(s):  
Organic Matter ◽  
Dry Matter ◽  
In Vitro Digestion ◽  
Barley Grain ◽  
Absolute Amount ◽  
In Vitro Digestibility ◽  
Promoting Effect ◽  
Digestion Technique ◽  
The Difference

Six wethers with rumen fistula were each fed a different combination of alfalfa hay, wheat straw, and barley grain. The same mixtures were fermented for periods of 12 and 48 hours by inoculum from each wether, using a two-stage in vitro digestion technique. The in vitro digestibility of dry matter and organic matter was determined on each treatment combination.The digestibility data revealed interactions among inoculum donor diets, in vitro substrate mixtures, and fermentation periods. When the diet lacked one or more of the substrate components, a 12-hour artificial rumen fermentation resulted in digestibilities that decreased as the composition of the substrate deviated increasingly from that of the donor diet. This effect was eliminated by extending the fermentation period to 48 hours. When all the substrate ingredients were included in the donor diet, the absolute amount of each ingredient, within 10 to 80%, was of less importance.Indications were that alfalfa possessed a specific growth-promoting effect on the microflora.Increasing the proportion of alfalfa in the substrate increased the difference between the in vitro digestibility of dry matter and organic matter. This was suggested to result from a higher concentration of soluble ash in the alfalfa fraction of the mixtures.

scholarly journals 127 Porcine in vitro digestibility of whole stillage predigested with multi-enzyme during different time periods

2020 ◽  
Vol 98 (Supplement_3) ◽  
pp. 49-50
Author(s):  
Kevin S Jerez Bogota ◽  
Tofuko A Woyengo
Keyword(s):  
Dry Matter ◽  
In Vitro Digestion ◽  
Enzyme Treatment ◽  
Optimal Time ◽  
In Vitro Digestibility ◽  
Percentage Points ◽  
Whole Stillage ◽  
Freeze Dried ◽  
Different Sources

Abstract A study was conducted to determine the effects of the period of predigesting whole stillage (WS; slurry material that is dried into DDGS) with multi-enzyme and composition of the multi-enzyme on porcine in vitro digestibility of dry matter (IVDDM) of the WS. Four samples of whole stillage from 4 different sources were freeze-dried and divided into 13 subsamples to give 52 sub-samples. Thirteen treatments were applied to the 48 sub-samples within source. The treatments were undigested WS (control); or pre-digested with 1 of 3 multi-enzymes (MTE1, MTE2, and MTE3) at 55 °C for 6, 12, 18 or 24 h in 3 × 4 factorial arrangement. The MTE1 contained xylanase, β-glucanase, cellulase, mannanase, protease, and amylase; MTE2 contained xylanase, α-galactosidase, and cellulase; and MTE3 contained xylanase, cellulase, β-glucanase, and mannanase. The 52 subsamples were subjected to porcine in vitro digestion. The IVDDM of untreated WS was 73.3%. The IVDDM increased (P&lt; 0.05) with an increase in the predigestion period. However, a rise in the predigestion period from 0 to 12 h resulted in greater (P&lt; 0.05) response in mean IVDDM than an increment in the predigestion period from 12 to 24 h (11 vs. 0.83 percentage points). Predigestion period and multi-enzyme type interacted on IVDDM such that the improvement in IVDDM between 0 and 12 hours of predigestion differed (P&lt; 0.05) among the 3 multi-enzyme types (13.3, 11.1, and 8.5 percentage points for MTE3, MTE2, and MTE1, respectively). The LS means by multi-enzyme treatment were modeled and resulted in unparallel curves (P&lt; 0.05). The estimated maximum response of IVDDM for MTE1, MTE2 and MTE 3 were 82.4%, 84.7% and 87.1% at 15.8, 13 and 13.1 hours, respectively. In conclusion, the optimal time of predigestion of WS with multi-enzymes (with regard to improvement in its IVDDM) was approximately 14 h.

scholarly journals Effect of Phosphate Fertilizer and Arbuscular Mycorrhizal Fungi on The Nutrient, Phosphateuptake and in Vitro Digestibility of Alfalfa

2016 ◽  
Vol 40 (3) ◽  
pp. 203 ◽  
Author(s):  
Bambang Suwignyo ◽  
Bela Putra ◽  
Nafiatul Umami ◽  
Cahyo Wulandari ◽  
Ristianto Utomo
Keyword(s):  
Organic Matter ◽  
Mycorrhizal Fungi ◽  
Dry Matter ◽  
Arbuscular Mycorrhizal ◽  
Phosphate Fertilizer ◽  
P Uptake ◽  
In Vitro Digestibility ◽  
P Fertilizer ◽  
Total P

This study aimed to determine the effect of arbuscular mycorrhizal fungi (AMF) and phosphate (P) fertilizer on the nutrient content, phosphate uptake and in vitro digestibility of alfalfa (Medicago sativa L.).The research was conducted at green house of Forage and Pastures Science Laboratory, Faculty of Animal Science Universitas Gadjah Mada. The experiment was arranged in Completely Randomized Design using 3x4 factorial patterns with four replications. The first factor was dosage of phosphate fertilizer SP 36 (0, 60, and 120 kg/ha). Second factor was the dosage of AMF (0, 0.8, 1.6, and 2.4kg/ha). The variable measured was nutrient contents (crude protein, dry matter, and organic matter), total P uptake and dry matter and organic matter in vitro digestibility. The results showed that the interaction of AMF and P fertilizer had no significant effect on crude protein and total P uptake, but highly significant effect on the parameters of dry matter, organic matter and dry matter and organic matter in vitro digestibility. 

scholarly journals The influence of the method of preservation of forages on the digestion in dairy cows. 1. Composition of the forages and digestibility of dry matter, organic matter and nitrogen.

1975 ◽  
Vol 23 (1) ◽  
pp. 3-9
Author(s):  
S. Tamminga ◽  
C.J. van der Koelen
Keyword(s):  
Organic Matter ◽  
Formic Acid ◽  
Dairy Cows ◽  
Crude Protein ◽  
Dry Matter ◽  
Mixed Diet ◽  
Apparent Digestibility

1. Grass from the same sward was ensiled without additive, with 14.6 g formic acid/100 g crude protein or 10.8 g formic acid and 10.6 g formaldehyde/100 g crude protein. Similar grass was dried and pelleted. Drying or ensiling with the mixture reduced solubility of N in the preserved grass but formic acid increased it, and ensiling without additive increased it even more. Apparent digestibility of N in the rumen of cows tended to decrease with decrease in solubility. Digestibility in vitro of the mixed diet given to the cows, calculated from digestibility of the separate components, agreed well with the values in vivo for diets with silages, but was high for that with dried grass. (Abstract retrieved from CAB Abstracts by CABI’s permission)

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