INTERACTION BETWEEN INOCULUM DONOR DIET AND SUBSTRATE IN IN VITRO RUMINANT DIGESTION STUDIES

1966 ◽  
Vol 46 (2) ◽  
pp. 91-95 ◽  
Author(s):  
J. E. Knipfel ◽  
J. E. Troelsen
Keyword(s):  
Organic Matter ◽  
Dry Matter ◽  
In Vitro Digestion ◽  
Barley Grain ◽  
Absolute Amount ◽  
In Vitro Digestibility ◽  
Promoting Effect ◽  
Digestion Technique ◽  
The Difference

Six wethers with rumen fistula were each fed a different combination of alfalfa hay, wheat straw, and barley grain. The same mixtures were fermented for periods of 12 and 48 hours by inoculum from each wether, using a two-stage in vitro digestion technique. The in vitro digestibility of dry matter and organic matter was determined on each treatment combination.The digestibility data revealed interactions among inoculum donor diets, in vitro substrate mixtures, and fermentation periods. When the diet lacked one or more of the substrate components, a 12-hour artificial rumen fermentation resulted in digestibilities that decreased as the composition of the substrate deviated increasingly from that of the donor diet. This effect was eliminated by extending the fermentation period to 48 hours. When all the substrate ingredients were included in the donor diet, the absolute amount of each ingredient, within 10 to 80%, was of less importance.Indications were that alfalfa possessed a specific growth-promoting effect on the microflora.Increasing the proportion of alfalfa in the substrate increased the difference between the in vitro digestibility of dry matter and organic matter. This was suggested to result from a higher concentration of soluble ash in the alfalfa fraction of the mixtures.

scholarly journals Note on in vitro digestion of avocado products for pigs

2021 ◽  
Vol 51 (1) ◽  
Author(s):  
J. Ly ◽  
P. Fránquez ◽  
G. Rodríguez ◽  
C. Lemus ◽  
I.A. Dominguez ◽  
...  
Keyword(s):  
Organic Matter ◽  
Dry Matter ◽  
Nutritional Value ◽  
In Vitro Digestion ◽  
In Vitro Digestibility ◽  
Optimal Production ◽  
Additional Protein

In vitro (pepsin/pancreatin) digestibility coefficients of pulp, seed, and peel of avocados of the Creole and Hass varieties were studied according to a 2 x 3 factorial arrangement in which the factors were the variety and parts of the fruit. Creole avocado were heavier in natura than Hass exemplars (310.7 g and 156.8 g), whereas Hass had a greater proportion of pulp than the Creole fruits (76.0% and 64.6%). No significant interactions were observed. In vitro digestibility of dry matter (DM), organic matter (OM), and nitrogen (N) were higher for the Creole variety compared with the Hass. In vitro digestibility of N was similar in the pulp and seeds, and higher than in the peels (731, 773, and 550 g/kg-1, respectively). Use of the entire avocado as pig feedstuff would have a lower nutritional value compared with feeding just avocado pulp. Supplementation of a diet based on avocado with additional protein may be needed for optimal production.

RELATIONSHIP BETWEEN IN VITRO DIGESTIBILITY AND FINENESS OF SUBSTRATE GRIND AS AN INDICATOR OF VOLUNTARY INTAKE OF HAY BY SHEEP

1969 ◽  
Vol 49 (1) ◽  
pp. 119-124
Author(s):  
J. E. Troelsen ◽  
J. M. Bell
Keyword(s):  
Organic Matter ◽  
Dry Matter ◽  
Grass Species ◽  
In Vitro Digestibility ◽  
Fine Grinding ◽  
Voluntary Intake ◽  
The Difference ◽  
Alfalfa Varieties ◽  
Relationship Of

Hay of two alfalfa varieties and four grass species harvested at six maturity stages each was fed to sheep. Voluntary intakes were measured in grams of dry matter daily per kg of metabolic size. Samples were ground coarsely and finely and digested in vitro. Fine grinding did not increase the digestibility of immature hay, but with advancing maturity the fine substrate was progressively more digestible than the coarse. This difference was inversely related (P < 0.05) to voluntary feed intake. The regression of voluntary intake of dry matter (g/day/kg0.75 body weight) on the difference of in vitro digestibility of organic matter in finely and coarsely ground substrate was −2.32 g in the alfalfa hay and −4.18 g in the grass hay. The coefficient of variation from regression was 14% in the alfalfa and 17% in the grass hays. Fine grinding had a greater effect on the in vitro digestibility of organic matter than of dry matter. This resulted in a higher significance of the relationship of intake with the effect of grinding on the digestibility of organic matter than on that of dry matter.

scholarly journals 127 Porcine in vitro digestibility of whole stillage predigested with multi-enzyme during different time periods

2020 ◽  
Vol 98 (Supplement_3) ◽  
pp. 49-50
Author(s):  
Kevin S Jerez Bogota ◽  
Tofuko A Woyengo
Keyword(s):  
Dry Matter ◽  
In Vitro Digestion ◽  
Enzyme Treatment ◽  
Optimal Time ◽  
In Vitro Digestibility ◽  
Percentage Points ◽  
Whole Stillage ◽  
Freeze Dried ◽  
Different Sources

Abstract A study was conducted to determine the effects of the period of predigesting whole stillage (WS; slurry material that is dried into DDGS) with multi-enzyme and composition of the multi-enzyme on porcine in vitro digestibility of dry matter (IVDDM) of the WS. Four samples of whole stillage from 4 different sources were freeze-dried and divided into 13 subsamples to give 52 sub-samples. Thirteen treatments were applied to the 48 sub-samples within source. The treatments were undigested WS (control); or pre-digested with 1 of 3 multi-enzymes (MTE1, MTE2, and MTE3) at 55 °C for 6, 12, 18 or 24 h in 3 × 4 factorial arrangement. The MTE1 contained xylanase, β-glucanase, cellulase, mannanase, protease, and amylase; MTE2 contained xylanase, α-galactosidase, and cellulase; and MTE3 contained xylanase, cellulase, β-glucanase, and mannanase. The 52 subsamples were subjected to porcine in vitro digestion. The IVDDM of untreated WS was 73.3%. The IVDDM increased (P&lt; 0.05) with an increase in the predigestion period. However, a rise in the predigestion period from 0 to 12 h resulted in greater (P&lt; 0.05) response in mean IVDDM than an increment in the predigestion period from 12 to 24 h (11 vs. 0.83 percentage points). Predigestion period and multi-enzyme type interacted on IVDDM such that the improvement in IVDDM between 0 and 12 hours of predigestion differed (P&lt; 0.05) among the 3 multi-enzyme types (13.3, 11.1, and 8.5 percentage points for MTE3, MTE2, and MTE1, respectively). The LS means by multi-enzyme treatment were modeled and resulted in unparallel curves (P&lt; 0.05). The estimated maximum response of IVDDM for MTE1, MTE2 and MTE 3 were 82.4%, 84.7% and 87.1% at 15.8, 13 and 13.1 hours, respectively. In conclusion, the optimal time of predigestion of WS with multi-enzymes (with regard to improvement in its IVDDM) was approximately 14 h.

scholarly journals Effect of Phosphate Fertilizer and Arbuscular Mycorrhizal Fungi on The Nutrient, Phosphateuptake and in Vitro Digestibility of Alfalfa

2016 ◽  
Vol 40 (3) ◽  
pp. 203 ◽  
Author(s):  
Bambang Suwignyo ◽  
Bela Putra ◽  
Nafiatul Umami ◽  
Cahyo Wulandari ◽  
Ristianto Utomo
Keyword(s):  
Organic Matter ◽  
Mycorrhizal Fungi ◽  
Dry Matter ◽  
Arbuscular Mycorrhizal ◽  
Phosphate Fertilizer ◽  
P Uptake ◽  
In Vitro Digestibility ◽  
P Fertilizer ◽  
Total P

This study aimed to determine the effect of arbuscular mycorrhizal fungi (AMF) and phosphate (P) fertilizer on the nutrient content, phosphate uptake and in vitro digestibility of alfalfa (Medicago sativa L.).The research was conducted at green house of Forage and Pastures Science Laboratory, Faculty of Animal Science Universitas Gadjah Mada. The experiment was arranged in Completely Randomized Design using 3x4 factorial patterns with four replications. The first factor was dosage of phosphate fertilizer SP 36 (0, 60, and 120 kg/ha). Second factor was the dosage of AMF (0, 0.8, 1.6, and 2.4kg/ha). The variable measured was nutrient contents (crude protein, dry matter, and organic matter), total P uptake and dry matter and organic matter in vitro digestibility. The results showed that the interaction of AMF and P fertilizer had no significant effect on crude protein and total P uptake, but highly significant effect on the parameters of dry matter, organic matter and dry matter and organic matter in vitro digestibility. 

scholarly journals Effect of Substitution of Urea With Different Type and Levels of Ruminant Manure on Nutritive Value of Rice Straw Silage

2019 ◽  
Vol 9 (2) ◽  
pp. 206-214
Author(s):  
Ali Ameen Saeed ◽  
Saja Intisar Abid
Keyword(s):  
Organic Matter ◽  
Rice Straw ◽  
Dry Matter ◽  
Nutritive Value ◽  
In Vitro Digestibility ◽  
N Content ◽  
Aerobic Stability

This study was conducted in Nutrition Lab. to investigate the effect of the type and level of substitution of urea with ruminant manure, M (sheep, cow and buffalo) on basis of nitrogen (N) content on the nutritive value of rice straw silage (RSS). Accordingly silages were nominated as, S-RSS, C-RSS and B-RSS. Urea (U) was substituted with dried manure at 6 combinations, 100:0, 90:10, 80:20, 70:30, 60:40 and 50:50 of U:M. Silage samples were prepared by treating chopped straw with pre-treated solution contained 10% low quality debis and 2% urea. Results showed that lower (P˂0.01) DM loss (11.4%) was observed in S-RSS, and with addition of urea only (3.6%).        Samples of S-RSS and C-RSS recorded higher (P˂0.01) Fleig points (Fp) as compared with those prepared by the addition of B-RSS, 60.42, 55.58 and 49.59 respectively. Reduction (P˂0.01) in this parameter was noticed in samples prepared with a combination of 100:0. Aerobic stability (AS) was a reduced (P˂0.01) in samples prepared by addition of S-RSS by 15 and 13 hours in comparison with samples of C-RSS and B-RSS respectively. Samples prepared with combination of 100:0 were prior (P˂0.01) as compared with other samples.        Results also showed an increase (P˂0.01) in in vitro digestibility of organic matter (IVOMD) in samples of S-RSS in comparison with samples prepared by addition of C-RSS and B-RSS, 49.99, 44.59 and 42.77% respectively. Samples prepared with combination of 100:0 recorded lower (P˂0.05) in vitro digestibility of dry matter (IVDMD) as compared with combinations of 70:30 and 60:40 of U: M, 40.52, 45.36 and 45.94% respectively.

Relationship between intake of silage and its chemical composition and in vitro digestibility

1972 ◽  
Vol 23 (1) ◽  
pp. 25 ◽  
Author(s):  
DC Brown ◽  
JC Radcliffe
Keyword(s):  
Dry Matter ◽  
In Vitro Digestion ◽  
Ammonia Nitrogen ◽  
Matter Content ◽  
In Vitro Digestibility ◽  
Dry Matter Content ◽  
Regression Analyses ◽  
Lactic Acids

Twenty experimental silages were made from seven pasture species at different stages of maturity. In vivo dry matter, organic matter, and energy ad libitum intakes and digestibilities of the silages were determined with standardized pairs of Merino wethers. The following chemical characteristics of the silages were measured: nitrogen, ammonia nitrogen, total titratable acids, acetic, propionic, butyric, and lactic acids, total volatiles lost during oven drying, lactic acid as a percentage of the total organic acids, pH, acid pepsin dry matter disappearance, dry matter content, and in vitro digestibility and rate of digestion. When all 20 silages were considered, energy intakes on a body weight basis were significantly related to silage pH (r = 0.55) and rate of in vitro digestion (r = 0.58). When the five legume silages were removed from the analysis and only the 15 grass-dominant silages were considered, dry matter intakes were significantly related to acetic (r = –0.57) and propionic acid (r = –0.55) concentrations. Multiple regression analyses did not significantly increase the accuracy of predicting intake. The results suggested that silage intake was negatively related to the degree of fermentation that occurred during the ensiling process.

The chemical composition and in vitro digestibility of pistachio by-product

2007 ◽  
Vol 2007 ◽  
pp. 223-223 ◽  
Author(s):  
Atiyeh Bohluli ◽  
Abasali Naserian ◽  
Reza Valizadeh ◽  
Fereydon Eftekarshahroodi
Keyword(s):  
Organic Matter ◽  
Chemical Composition ◽  
Total Phenolic Content ◽  
Dry Matter ◽  
Phenolic Content ◽  
Total Phenolic ◽  
In Vitro Digestibility ◽  
Pistachio Nuts ◽  
The World

Pistachio (pistachio vera) is from Anacardiaceae family. According to the FAO (2003) report, Iran is the largest pistachio producer in the world (more than 310,000 tons). The most three important exported pistachio nuts of Iran are Ohadi, Akbari and Kaleghuchi (Mohammadi, 2005). About 150,000 tons in DM of pistachio by-product (PB) is produced from dehulling process in Iran, annually. This by-product is mainly consisted of pistachio hulls (PH), and then peduncles, leaves and a little amount of mesocarp and kernels. In this experiment the chemical composition, Total Phenolic Content (TPC) and tannin amount of PB and PH of Ohadi, Kaleghuchi and white varieties were determined; also, In Vitro Dry Matter and Organic Matter Digestibility (IVDMD and IVOMD) were measured.

scholarly journals Sunflower cake in diets for beef cattle: digestibility, kinetics and in vitro ruminal fermentation parameters

2018 ◽  
Vol 41 (1) ◽  
pp. 39492 ◽  
Author(s):  
Rafael Henrique de Tonissi e Buschinelli de Goes ◽  
Luiz Henrique Xavier da Silva ◽  
Tatiana García Díaz ◽  
Antonio Ferriani Branco ◽  
Ana Lúcia Teodoro ◽  
...  
Keyword(s):  
Organic Matter ◽  
Beef Cattle ◽  
Soybean Meal ◽  
Crude Protein ◽  
Dry Matter ◽  
In Vitro Digestibility ◽  
Ruminal Fermentation ◽  
Fermentation Products ◽  
Sunflower Cake

 The objective of this study was to evaluate the effect of the inclusion of sunflower cake replacing soybean meal in beef cattle diets on the in vitro digestibility of dry matter (IVDDM), organic matter (IVDOM), crude protein (IVDCP) and the ruminal fermentation kinetics and parameters. The experiment was analyzed according to a completely randomized design. The treatments consisted of four levels of sunflower cake, 0, 200, 400, 600 g kg-1, replacing soybean meal in the concentrate of beef cattle diets. The coefficients of IVDDM, IVDOM and IVDCP presented a quadratic effect with the addition of sunflower cake. The soluble fraction (fraction B) degradation rate and total gas production decreased linearly with the inclusion of sunflower cake. Values of pH in ruminal fluid were higher for levels 0, 200 and 600 g kg-1 sunflower cake. Sunflower cake can replace soybean meal by up to 280 g kg-1 in the concentrate of beef cattle diets, improving the in vitro digestibility of dry matter organic, matter and crude protein. Levels above 400 g kg-1 reduce ruminal digestion rate, digestibility and release of final fermentation products. 

Determination of the yield characteristics and in vitro digestibility of barley forage harvested in different vegetation periods

2016 ◽  
Author(s):  
Mehtap Guney ◽  
Cagri Kale ◽  
Duran Bolat ◽  
Suphi Deniz
Keyword(s):  
Organic Matter ◽  
Dry Matter ◽  
Energy Content ◽  
Vegetation Period ◽  
Metabolizable Energy ◽  
Mature Stage ◽  
In Vitro Digestibility ◽  
Net Energy ◽  
Seed Formation

This study planned to determine the differences among nutrient composition, in vitro digestibility, energy content, digestible dry matter and organic matter yields of barley forage harvested at three different stages of maturity. Each vegetation period (heading stage, seed formation stage and mature stage) was randomly assigned to 5 replication from 1 square meter area and fifteen samples were harvested in total. DM, ADF (p<0.001), and NDF (p<0.05) contents were different in each stages of barley forage. In vitro dry matter (IVDMD), organic matter digestibility (IVOMD), metabolizable energy (ME), and net energy for lactation (NEL) values of samples were determined to be lower than the other two stages at the mature stage (p<0.05). Yield parameters of barley were significantly affected by vegetation period (p<0.001). It can be concluded that all three vegetation period had significantly higher digestibility. Digestible DM, OM and energy yields were higher when harvested at the mature stage of vegetation.

The influence of adaptation of rumen microflora on in vitro digestion of different forages by sheep and red deer

2002 ◽  
Vol 80 (11) ◽  
pp. 1930-1937 ◽  
Author(s):  
Iain J Gordon ◽  
F Javier Pérez-Barbería ◽  
Paloma Cuartas
Keyword(s):  
Cervus Elaphus ◽  
Red Deer ◽  
In Vitro Digestion ◽  
Ovis Aries ◽  
In Vitro Digestibility ◽  
Rumen Microbes ◽  
Digestion Technique ◽  
Digestion Efficiency ◽  
Rumen Microflora

The rumen microflora ecosystem adapts to the diet consumed by the animal. We tested the extent to which this adaptation facilitates or retards the digestion of plant-based forages. Following adaptation of sheep (Ovis aries) and red deer (Cervus elaphus) to diets containing different mixtures of alfalfa, grass, and heather (a dwarf shrub), an in vitro digestion technique was used to compare the ability of the rumen microflora to digest the mixtures of substrates to which they were adapted with their ability to digest different mixtures of the same substrates. In vitro digestion of different substrates was slightly greater in rumen liquor derived from sheep than in that derived from red deer for each of the different substrates, but the effect was not significant. Digestibility in sheep was independent of how the feed was presented (diet of equal proportions of alfalfa, grass, and heather in each meal (D-EQ): mean in vitro digestibility = 37.3%; alfalfa, grass, and heather presented sequentially on different days (D-SEQ): mean in vitro digestibility = 37.7%, SE of differences = 1.30%, p > 0.05). However, in red deer there was a significant effect of method of diet presentation (D-EQ: mean in vitro digestibility = 36.9%; D-SEQ: mean in vitro digestibility = 34.2%, SE of differences = 1.30%, p < 0.05), digestibility being substantially lower for D-SEQ than for D-EQ. Overall, the results demonstrated that whilst there were no species-specific differences in overall digestion efficiency, dietary adaptation had an effect on substrate digestion efficiency, with rumen microbes adapted to high-quality diets digesting these more efficiently than low-quality diets.

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