scholarly journals MUTU MIKROBIOLOGIS MINUMAN JAJANAN DI SEKOLAH DASAR WILAYAH BOGOR TENGAH

2007 ◽  
Vol 1 (1) ◽  
pp. 44
Author(s):  
Dwi Ariyani ◽  
Faisal Anwar
Keyword(s):  
High Risk ◽  
Microbial Contamination ◽  
Microbiological Quality ◽  
Coconut Milk ◽  
Plate Count ◽  
Analysis Method ◽  
Total Plate Count

<p class="MsoBodyTextIndent" style="margin: 0cm 12.6pt 6pt 18pt;"><span lang="en-us" xml:lang="en-us">The objectives of the study were to improve the microbiological quality of beverages that significantly affected by the type of selling unit and microbiological quality. The study was carried out from March  to  May 2004.  Three major location were selected, namely : SD Panaragan, SD Empang, and SD Papandayan, Bogor Tengah subdistrict. Determination analysis method of Total Plate Count (TPC) was used. The result of the study showed that beverages with a high risk of microbial contamination at SD Panaragan are “es doger”, “es campur”, and  “es kelapa”, most of these beverages contain coconut milk; at SD Empang are es jeruk and es kemasan; and  at SD Papandayan are es doger, es sirsak, es kemasan and, es teh. Microbiological quality of beverages sold by streetvendor were significantly affected by the types of selling unit. </span></p>

scholarly journals Microbiological quality of feed materials used between 2009 and 2012 in Poland

2013 ◽  
Vol 57 (4) ◽  
pp. 535-543 ◽  
Author(s):  
Elżbieta Kukier ◽  
Magdalena Goldsztejn ◽  
Tomasz Grenda ◽  
Krzysztof Kwiatek ◽  
Łukasz Bocian
Keyword(s):  
Microbial Contamination ◽  
Microbiological Quality ◽  
Plate Count ◽  
Aerobic Bacteria ◽  
The Past ◽  
Total Plate Count ◽  
Food And Feed ◽  
Plate Counts ◽  
Materials Used

Abstract The study was conducted at all regional veterinary diagnostic laboratories. Feed materials were examined for Salmonella prevalence and contamination by Enterobacteriaceae, aerobic mesophilic bacteria, total plate count, fungi, Clostridium sp., and Bacillus cereus. Assays were done following international and Polish standards used in food and feed microbiology. Salmonella sp. were most often detected in oil seeds. In most of the examined feed ingredients, the number of Enterobacteriaceae did not exceed 10 cfu/g. The contamination by aerobic bacteria ranged most often from 101to 107 cfu/g, and the highest mycological contamination was noted in cereal grains (108 cfu/g). The results showed that microbial contamination of feed materials in regard to Enterobacteriaceae, fungi, and total plate counts declined over the past years.

scholarly journals The effect of keeping technology on the microbiological status of raw milk

2008 ◽  
pp. 67-75
Author(s):  
Ferenc Peles ◽  
Zsuzsa Máthéné Szigeti ◽  
Béla Béri ◽  
András Szabó
Keyword(s):  
Escherichia Coli ◽  
Microbiological Quality ◽  
Raw Milk ◽  
Housing System ◽  
Plate Count ◽  
Small Farms ◽  
Total Plate Count ◽  
Large Farms ◽  
Microbiological Status

The importance of the quality of raw milk increased after Hungary had joined to the EU. On delivery of raw milk, the microbiological quality, especially total plate count of the milk is very important. Twenty-two farms (7 large, 4 medium-sized, and 11 small farms) were included in the study. We considered the different farm size, keeping- and milking circumstances during the selection of farms. The examined large farms use loose housing system (cubicle, deep litter) and milking parlour. Most of them use preand post-milking disinfection. In the medium-sized farms, loose,deep litter and tie-stall housing system, as well as milking parlour, pipeline milking and bucket milking occurred. All of them use preand post-milking disinfection. Small farms use tie-stall housing system, bucket milking and udder preparation by water. Unfortunately, they do not use pre- or post-milking disinfection. In the large and medium-sized farms mainly Holstein Friesian, in the small farms Hungarian Simmental breeds can be found.The aim of our research was to examine the microbiological status of the raw milk produced in dairy farms (total plate count, coliform count, Escherichia coli count, Staphylococcus aureus count, psychrotroph bacteria count, furthermore yeast and mold count); sources of the contamination; connection between the microbiological quality of produced milk and housing-, milking technologies of farms; furthermore the hygienic circumstances of milking and milk handling of the farms, by the examination of coliform bacteria and Escherichia coli contamination.During the examination of the connection between the different farm sizes, various housing- and milking forms and the microbiological characteristics we observed similar tendencies in the case of total plate count, coliform count, yeast and molds count, furthermore psychrotroph bacteria count. The value of  these parameters was significantly higher in small farms, and infarms which use tie-stall housing forms, bucket milking, udder preparation with water, and which do not use pre- and post-milking disinfection.The results showed that besides cooling, the milking procedure and the type of udder preparation had the largest effect on the total plate count. Statistical analysis shows that in medium and small farms the combination of pipeline milking – tie stall housing system – disinfectant preparation of the udder; in large farms the combination of milking parlour – loose cubicle housing system – dry preparation of the udder are the most appropriate in the aspect of the total plate count. We experienced that in farms where the hygienic instructions are not followed – and thereforeequipment used during the milking and handling of milk is very contaminated – or rather the separation of mastitic cows’ milk is not appropriate, different microorganisms may contaminate the produced milk. 

scholarly journals The Effect of Active Paper Packaging Enriched with Oleoresin from Solid Waste of Pressed Curcuma xanthorrhiza Roxb. Placement Methods on Quality of Refrigerated Strawberry (Fragaria x ananassa)

2021 ◽  
Vol 36 (1) ◽  
pp. 155
Author(s):  
Rohula Utami ◽  
Kawiji Kawiji ◽  
Windi Atmaka ◽  
Lulus Nurmaya ◽  
Lia Umi Khasanah ◽  
...  
Keyword(s):  
Solid Waste ◽  
Microbiological Quality ◽  
Fragaria X Ananassa ◽  
Soluble Solids ◽  
Plate Count ◽  
Refrigerated Storage ◽  
Titratable Acid ◽  
Total Plate Count ◽  
Curcuma Xanthorrhiza

Strawberry is a popular and nutritious fruit. However, its improper post-harvest handling leads to quality degradation. The aim of this study was to investigate the effects of active paper packaging placements methods on strawberry (<em>Fragaria x ananassa</em>) quality during refrigerated storage. Strawberry quality was determined based on the physical (weight loss, hardness, color), chemical (pH, total titratable acid, total soluble solids and vitamin C content) and microbiological (total plate count) properties. The results of this study indicated that different placement methods of active paper packaging had no significant effect on the physical and chemical qualities; but it did affect the microbiological quality of the strawberries. At the end of the experiment (12 days), the microbial content of C (the bottom and walls), D (covering all surfaces) and E (cut into pieces) samples were significantly lower than the A (without active paper packaging) and B (the bottom) samples. The Total Plate Count (TPC) values of the control and B samples were higher than the maximum acceptable limit of 6 log10 colony-forming units (CFU) g<sup>-1</sup>, while the other samples remained below the limit. These results indicated that a larger surface area of active paper that had contact with the strawberries provided better protection against microbes. Due to their microbial properties, C, D and E treatments maintained the strawberry quality for up to 12 days, compared with 9 days for control and B treatments. Active paper packaging containing oleoresin from solid waste of pressed <em>Curcuma xanthorrhiza</em> Roxb. can be applied to extend the shelf life of strawberries during refrigerated storage.

Microbial quality of frozen squid (Sepioteuthis lessoniana, Lesson 1830) treated with food grade commercial chemicals

2015 ◽  
Author(s):  
Vikas Kumar ◽  
D. Sukumar ◽  
M. Muruganantham
Keyword(s):  
Quality Evaluation ◽  
Microbiological Quality ◽  
Plate Count ◽  
Microbial Quality ◽  
Food Grade ◽  
E Coli ◽  
Total Plate Count ◽  
Sepioteuthis Lessoniana ◽  
Better Than

Squids perish rapidly like other fishes and require some treatment to maintain the quality for export. The microbial quality of frozen squid (Sepioteuthis lessoniana, Lesson 1830) treated with the commercial food grade chemicals imported from Spain was therefore studied. Dressed squids were treated with the chemicals Hidratech_4A (0.4%) and Whitech_3 (0.25%) dissolved in chilled freshwater (STCF) and chilled saltwater (STCS). Chilled squids not treated served as control (SNTC). Squids were quick frozen at -40°C in contact plate freezer and stored at-20±1°C. Samples were tested raw, after pre-processing, treatments and freezing and during storage at monthly intervals for 7 months. The microbial quality evaluation included Total Plate Count (TPC), Escherichia coli, Staphylococcus aureus, Vibrio cholerae and Salmonella. Study revealed a better quality of treated samples than control. Microbiological quality of STCS was better than STCF and SNTC. E. coli counts decreased after treatment. Salmonella and V. cholerae were absent.

scholarly journals Microbiological quality evaluation of ready-to-eat mixed vegetable salad, food ingredients and some water samples from a restaurant in Accra: A case study

2020 ◽  
Vol 20 (06) ◽  
pp. 16669-16688
Author(s):  
Nii Korley Kortei ◽  
◽  
T Annan ◽  
L Quansah ◽  
G Aboagye ◽  
...  
Keyword(s):  
Public Health ◽  
Water Samples ◽  
Microbial Contamination ◽  
Microbiological Quality ◽  
Food Microbiology ◽  
Plate Count ◽  
Food Ingredients ◽  
Economic Implications ◽  
Microbiological Methods

One serious threat to public health in both developed and developing countries is the microbial contamination of food. This problem poses a great challenge and consequently has economic implications. Causes of microbial contamination are diverse and these may be natural, environmental, or technological. The microbiological quality of most ready-to-eat foods is of great significance to human health because they require minimal or no processing when consumed.The aim of this research was to investigate the microbiological quality of some ready-to-eat mixed vegetable salad foods, ingredients as well as the wash water samples of an urban restaurant located in Accra, Ghana. A total of thirty (30) samples categorized into mixed vegetable salads, foods and water obtained from an urban restaurant in the national capital of Ghana, Accra. They were analyzed at the microbiology laboratory and food microbiology laboratories of School of Allied Health Sciences(UHAS)and Council for Scientific and Industrial Research-Food Research Institute (CSIR-FRI), Ghana,respectively. Standard microbiological methods that are per International Organization for Standardization(ISO) Methods and Nordic Committee on Food Analysis Methods (NMKL) were used in determining the presence and levels of bacteria and fungi.Data obtained were transformed from standard to logarithmic forms and reported as mean+standard deviations. The aerobic plate count samples ranged from 0-4.73 log 10CFU/g. E-coli counts also ranged between 0-2.53 log 10CFU, while Bacillus cereus counts were very low at0-<10log 10CFU/g. Clostridium perfringens and Staphylococcus aureus counts were also very low ranging from0-1.0log10CFU/g.Enterobacteriaceae counts also ranged from 0-1.90 log10CFU/g. Molds and yeasts counts were generally low and ranged from 0-2.48 log 10CFU/g and 0-1.0 log10CFU/g,respectively. None of the samples tested contained Listeria monocytogenes and Salmonella spp. Fungal microbial loads were minimal given the quantities, and were deleterious to the health of consumers. The study revealed that the bacterial loads on mixed vegetable salads, ingredients and water samples used and served by an urban restaurant in Accra were within safe limits according to American Public Health Association (APHA) and International Commission for Microbiological Specifications for Foods (ICMSF) guidelines and,therefore,good for human consumption.

scholarly journals Microbiological and formaline test on the big eye tuna (Thunnus obesus Lowe, 1839) from fish auction place (TPI) and moving fish trader (PIK) in Panimbang Pandeglang Village Banten

2021 ◽  
Vol 1 (1) ◽  
pp. 38-45
Author(s):  
Lia Amelia Pertiwi ◽  
Hadi Susilo ◽  
Nurullah Asep Abdilah
Keyword(s):  
Microbial Contamination ◽  
Brilliant Green ◽  
Economic Value ◽  
Plate Count ◽  
E Coli ◽  
Plate Count Agar ◽  
Total Plate Count ◽  
Thunnus Obesus ◽  
The World

Big Eye Tuna (Thunnus obesus Lowe, 1839) is one of the fish species that can increase sources of animal protein and has high economic value in the world of trade because it is the second-largest export commodity after shrimp. The purpose of this study was to test the content of microbial and formalin contamination in the flesh of T. obesus fish from the Fish Auction Place (TPI) and Mobile Fish Trader (PIK) in Panimbang Village, Pandeglang, Banten. The research was carried out at the Regional Technical Implementation Unit (UPTD) Testing and Application of Quality of Fishery Products, Department of Marine Affairs, and Fisheries of Banten Province. This research is a descriptive laboratory study with purposive sampling. Twelve samples of T. obesus fish obtained from TPI (6 fishes) and PIK (6 fishes) were taken for 25 g of meat. The tested for microbial contamination content with Total Plate Count (TPC) using Butterfield's phosphate (BFP) media, and Plate Count Agar (PCA), Coliform-Test, and E. coli-Test using Lauryl Tryptose Broth (LTB). Brilliant Green Lactose Bile (BGLB), EC Broth and Levine's Eosin Methylene Blue (LEMB), and Formaldehyde-Test using  Formaldehyde-Test Kits. The results showed that the flesh of T. obesus fish contained microbial contamination with the average values ​​of TPC, Coliform MPN, and E. coli MPN, respectively, namely 1.6 103 colony/g, 15.2 MPN/g and < 3 MPN/g ( TPI), and 1,7103 colony/g, 61.3 MPN/g and < 3 MPN/g (PIK). Therefore, fish in TPI and PIK are safe for consumption as stipulated in SNI.

Microbiological safety of ready-to-eat foods in hospital and university canteens in Hanoi, Vietnam

2021 ◽  
Author(s):  
Hue Thi Luu ◽  
Chris M. Michiels
Keyword(s):  
Microbiological Quality ◽  
Intervention Strategies ◽  
Plate Count ◽  
Quality And Safety ◽  
Baseline Measurement ◽  
E Coli ◽  
Microbiological Safety ◽  
Total Plate Count ◽  
And Staphylococcus Aureus

The aim of this study is to analyze and document the microbiological safety and quality of ready-to-eat foods in hospital and university canteens in Hanoi, Vietnam. A total of 420 ready-to-eat food products from 21 canteens were sampled in July 2018 and May 2019. The ratio of samples exceeding the unsatisfactory level for Total Plate Count (TPC) was 31%. Escherichia coli, Listeria and Staphylococcus aureus were detected in 35 (8.3%), 99 (24%), 46 (11%) samples, with 3%, 10% and 0% exceeding the unsatisfactory level, respectively. The Total Plate Count (TPC), Listeria, Bacillus cereus, E. coli, S. aureus ranged from below detection limit to 5x10 9 , 4.6x10 5 , 6.2x10 3 , 3.4x10 3 , 7.6x10 3 CFU/g, respectively. Listeria monocytogenes was isolated from 3/420 samples (0.7%). In addition, there were 21 out of 410 samples (5%) contaminated with Salmonella. Overall, our data indicate frequent problems with the microbiological quality and safety of these canteen foods in Hanoi, and provide a baseline measurement that will allow environmental health officers and food microbiologists to develop targeted intervention strategies to reduce the economical and public health risk associated with these foods.

Microbiological Quality of Fresh-Squeezed Orange Juice and Efficacy of Fruit Surface Decontamination Methods in Microbiological Quality

2011 ◽  
Vol 74 (8) ◽  
pp. 1238-1244 ◽  
Author(s):  
UFUK BAGCI ◽  
AYHAN TEMIZ
Keyword(s):  
Orange Juice ◽  
Microbial Contamination ◽  
Microbial Population ◽  
Microbiological Quality ◽  
Boiling Water ◽  
Plate Count ◽  
E Coli ◽  
Surface Decontamination ◽  
Fruit Surface

The aims of this study were to evaluate the microbiological quality of fresh-squeezed orange juice and to reduce the microbial population by using various chemical and physical fruit surface decontamination methods. In the first step of the study, polyethylene-bottled fresh-squeezed orange juice samples purchased in Ankara, Turkey, were examined. The average aerobic plate count (APC) and coliform count (CC) varied within the ranges of 3 to 5 log CFU/ml and 1 to 4 log MPN/ml, respectively. Ten of 60 samples contained various levels of Escherichia coli, while Salmonella spp. and E. coli O157:H7 were not detected in any of the samples. Comparing the efficacy of various fruit surface decontamination methods on microbial population of oranges, the best results were obtained following two applications of submersion in boiling water and 5% H2O2 solution for both the uninoculated and inoculated samples. Orange juice samples obtained from surface-inoculated and decontaminated oranges were also examined. We showed that about 17.4% of the E. coli population was transferred to orange juice after extraction, indicating the separation of microbial contaminants from fruit peel during extraction. Finally, the levels of microbial contamination occurred throughout the extraction process on the inner surfaces of a commercial juice extractor at one of the sale points investigated. Significant (P &lt; 0.05) increases in the APC and CC were determined in surface samples of the extractor after the extraction. Surface decontamination and extraction are critical steps in fresh juice production for preventing microbial contamination. Immersion in boiling water for 0.5 min, without using any chemicals, can be offered as an effective method to reduce microbial population on orange surfaces.

scholarly journals PENGARUH KONDISI HOMOGENISASI TERHADAP KARAKTERISTIK FISIK DAN MUTU SANTAN SELAMA PENYIMPANAN

2020 ◽  
Vol 18 (1) ◽  
pp. 31
Author(s):  
SARI INTAN KAILAKU ◽  
TATANG HIDAYAT ◽  
DONDY A. SETIABUDY
Keyword(s):  
Optimal Condition ◽  
Storage Period ◽  
Coconut Milk ◽  
Plate Count ◽  
Tween 20 ◽  
Practical Reasons ◽  
Homogenization Process ◽  
Total Plate Count ◽  
Randomized Design

<p>ABSTRAK<br />Produk santan awet semakin banyak digunakan masyarakat untuk<br />alasan kepraktisan. Salah satu cara untuk menstabilkan emulsi santan yaitu<br />dengan penambahan emulsifier/stabiliser dan proses homogenisasi.<br />Penelitian ini bertujuan untuk mendapatkan kondisi proses homogenisasi<br />yang dapat menghasilkan emulsi santan yang stabil dengan masa simpan<br />yang optimal. Perlakuan yang diterapkan yaitu tiga kecepatan putaran<br />(6.000, 11.000, dan 16.000 rpm) dan empat durasi homogenisasi (10, 20,<br />30, dan 40 menit). Percobaan menggunakan rancangan acak lengkap<br />(RAL) faktorial dengan tiga kali ulangan. Parameter yang diamati yaitu<br />karakteristik fisik emulsi santan yang meliputi stabilitas emulsi, viskositas,<br />dan mikroskopik santan. Kemudian diamati pula mutu santan selama<br />penyimpanan dengan menguji parameter pH, warna, kadar asam lemak<br />bebas, dan total mikroba. Emulsifier yang digunakan adalah Tween 20<br />(konsentrasi 0; 0,5; dan 1%) dan karboksi-metilselulosa-CMC (konsentrasi<br />0 dan 0,6%). Hasil penelitian menunjukkan bahwa penggunaan CMC 0,6%<br />dapat menghasilkan stabilitas emulsi yang baik (100%) sampai dengan<br />akhir pengamatan (14 hari). Kondisi proses homogenisasi yang optimal<br />diperoleh pada kecepatan putaran 6.000 rpm selama 30 menit. Mutu santan<br />yang dihasilkan dari kondisi proses homogenisasi yang optimal cukup baik<br />dan layak dikonsumsi sampai dengan penyimpanan minggu ke-6.<br />Kata kunci: Santan, homogenisasi, emulsifier/stabiliser, karboksi-metil-<br />selulosa (CMC), mutu</p><p>ABSTRACT<br />Preserved coconut milk is increasingly used publicly because of its<br />practical reasons. One of the ways to stabilize the coconut milk emulsion<br />is by addition of emulsifier/stabilizer and homogenizing process. This<br />study aimed at obtaining conditions of the homogenization process to<br />produce stable emulsions of coconut milk with optimum storage period.<br />The treatments applied were three rotation speeds (6,000, 11,000, and<br />16,000 rpm) and four homogenization durations (10, 20, 30, and 40<br />minutes). The experiment was arranged using factorial completely<br />randomized design (CRD) factorial with three replicates .  Parameters<br />observed were physical characteristics including coconut milk emulsion<br />stability, viscosity, and microscopic test of milk. Furthermore, it was then<br />observed quality of the coconut milk during storage by testing the<br />parameters of pH, color, free fatty acid (FFA) levels, and total microbes by<br />total plate count (TPC). Emulsifiers used in the experiment were Tween 20<br />(concentrations of 0, 0.5, and 1%) and carboxy-methylcellulose - CMC<br />(concentrations of 0 and 0.6%). The results showed that the use of 0.6%<br />CMC was able to produce a good emulsion stability (100%) until the end<br />of the observation (14 days). Optimal condition of homogenization<br />process was obtained at the rotation speed of 6,000 rpm for 30 minutes.<br />The quality of coconut milk resulted from optimal condition of<br />homogenization process was quite good and valid for consumption up to 6<br />weeks of storage.<br />Key words : Coconut  milk,  homogenization,  emulsifier/stabilizer,<br />carboxy-methylcellulose (CMC), quality.</p>

scholarly journals Microbiological quality of undulated surf clam (Paphia undulata) in selected areas in Samar, Philippines

2021 ◽  
Vol 32 (2) ◽  
pp. 213-220
Author(s):  
KRISANTO L. BACNUTAN ◽  
KIRBY ULYSSES M. MOMO ◽  
RYAN JAMES A. PAGTABUNAN ◽  
JERSON SORIO
Keyword(s):  
Drug Administration ◽  
Microbiological Quality ◽  
Plate Count ◽  
Microbial Quality ◽  
Purification Process ◽  
Surrounding Water ◽  
Surf Clam ◽  
Total Plate Count ◽  
Filter Feeders

Bivalves are filter feeders that ingest particles from the surrounding water, including pathogens,which could cause illness in consumers. In Samar, Philippines no data on the microbial quality of undulatedsurf clam (Paphia undulata) have previously been reported. Thus, this study was conducted to determine themicrobial quality of the species in selected areas in Samar, Philippines (Pinabacdao, Zumarraga andVillareal). The total plate count (TPC) and total Vibrio count were beyond the standard limits set by the Foodand Drug Administration (FDA), although Salmonella was not detected in any samples. The TPC ofundulated surf clam was 5.9-6.3 log CFU·g-1 in Pinabacdao, 4.9-6.0 log CFU·g-1 in Zumarraga, and 6.3-7.2log CFU·g-1 in Villareal. The total Vibrio count was 5.1-5.7 log CFU·g-1 in Pinabacdao, 3.9-4.6 log CFU·g-1in Zumarraga, and 6.1 log CFU·g-1 in Villareal. Bivalves in these areas should undergo purification process,such as relaying or depuration

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