Antihypertension Effect from Bakasang’s Peptide Extract Based on MDA Levels in Sera and iNOS Expression in Cardiac Tissue of Rats Hypertensive Model

2017 ◽  
Vol 9 (2) ◽  
Author(s):  
Hilman Nurmahdi ◽  
Sasangka Prasetyawan ◽  
Max Robinson Wenno ◽  
Aulanni’am Aulann’am
Keyword(s):  
Blood Pressure ◽  
Angiotensin Ii ◽  
Cardiac Tissue ◽  
Genetic Disorder ◽  
Negative Control Group ◽  
Negative Control ◽  
Inos Expression ◽  
Control Group ◽  
Katsuwonus Pelamis ◽  
Group 2

Hypertension is an abnormality of cardiovascular condition, marked by high-blood pressure over normal condition. One of the factors that causes hypertension is altered equilibrium of Renin-Angiotensin-Aldosteron system, which commonly caused by genetic disorder, could produces excess of Angiotensin II which known as vasoconstrictor. Angiotensin II elevates body’s blood pressure and stimulates production of free radicals causes hypertension and oxidative stress. This research conducted to explore potential antihypertension effect from bakasang’s peptide extract made from fermented skipjack tuna (Katsuwonus pelamis L.) based on MDA levels in sera and iNOS expression in cardiac tissue from hypertension rats (Rattus norvegicus) induced by DOCA-salt. Rats were divided into five groups, they were: (1) negative control group, (2) hypertensive control group, (3) hypertension with captopril therapy with dose of 5 mg/kg of body weight (BW), (4,5) bakasang’s peptide extract therapy with dose of 200 mg/kgBW, and 300 mg/kgBW respectively. The results showed that bakasang’s peptide extract with dose of 200 mg/kgBW significantly decrease (p less than 0.05) MDA level in sera and iNOS expression in cardiac tissue. MDA level decreased up to 32.77%, and iNOS level of expression decreased up to 34.46 % after therapy using bakasang’s peptide extract on hypertension rats. It can be concluded that bakasang’s peptide extract has antihypertension effect (ACE-inhibitor), and capable to decrease MDA levels in sera and iNOS expression in cardiac tissue on hypertension rats induced by DOCA-salt.

The Antihypertension Effect of Fermented Skipjack Tuna (Katsuwonus pelamis L.)/Bakasang’s Peptide Extract Based on Cardiac’s Histopathology and Protease Activity on Hypertensive Rats Induced by Deoxycorticosterone Acetate (DOCA) -Salt

2017 ◽  
Vol 9 (1) ◽  
Author(s):  
Hilman Nurmahdi ◽  
Dyah Kinasih Wuragil ◽  
Sasangka Prasetyawan ◽  
Aulanni’am Aulann’am
Keyword(s):  
Blood Pressure ◽  
Angiotensin Ii ◽  
Protease Activity ◽  
Cardiac Tissue ◽  
Negative Control Group ◽  
Negative Control ◽  
Control Group ◽  
Skipjack Tuna ◽  
Hypertensive Rats ◽  
Katsuwonus Pelamis

Hypertension is an abnormality of cardiovascular condition, marked by high-blood pressure over the normal condition. One of the factors that causes hypertension is altered equilibrium of Renin-Angiotensin-Aldosteron system (RAAS). This condition, which commonly by genetic could causes excess production of Angiotensin II, which known as vasoconstrictor, carried out by Angiotensin Converting Enzyme (ACE). Angiotensin II could increases the body’s blood pressure, and stimulates the production of free radicals, causing hypertension and oxidative stress. One of the common medication for hypertensive patient are using ACE inhibitor pills. Consumption of commercial pills, had a negative effect on the patient, such as skin iritation, cough, allergic reaction, etc. This study was conducted to explore the potential peptide of bakasang extract isolated from fermented skipjack tuna (Katsuwonus pelamis L.) for antihypertensive therapy, based on the activity of protease and histopathology of cardiac tissue on hypertensive rats (Rattus norvegicus) induced by DOCA-salt. The rats were divided into five groups: (1) negative control group, (2) hypertensive control group, (3) hypertension with captopril therapy dose of 5 mg/kg of body weight (BW), (4) and (5) hypertension with bakasang’s peptide extract therapy dose of 200 mg/kgBW, and 300 mg/kgBW, respectively. The results showed that bakasang’s peptide extract dose of 200 mg/kgBW significantly (p less than 0.05) decrease protease activity and improve histopathology of cardiac tissue on hypertensive rats. Protease activity post-therapy decreased to be 39.27 % from positive hypertension group. It could be concluded that the peptide of bakasang’s peptide extract has antihypertension effect (ACE-inhibitors), which was able to improve histopathology and decrease protease activity of the cardiac tissue on hypertensive rats induced by DOCA-salt.

Antiurolithiatic effect of Cymbopogon Proximus, Alhagi Maurorum, on Sulfadimidine induced urolithiasis in male New Zealand rabbits

2019 ◽  
Vol 20 (1) ◽  
pp. 12-18
Author(s):  
Sameh El-Nabtity
Keyword(s):  
New Zealand ◽  
Intramuscular Injection ◽  
Negative Control Group ◽  
Negative Control ◽  
Control Group ◽  
Aqueous Extracts ◽  
Blood And Urine Samples ◽  
Group 2 ◽  
New Zealand Rabbits ◽  
Group 1

The present study aimed to investigate the prophylactic effect of Cymbopogon proximus and Alhagi maurorum on Sulfadimidine induced urolithiasis in rabbits . Thirty New Zealand male rabbits were allocated into six equal groups (each of five): Group (1) was used as a negative control. Group(2) were administered sulfadimidine (200mg/kg) by intramuscular injection.Groups(3) and (4) were administered sulfadimidine(200mg/kg) by intramuscular injection and 330mg/kg of Cymbopogon proximus alcoholic and aqueous extracts respectively orally.Groups(5) and (6) were administered sulfadimidine(200mg/kg) by intramuscular injection and 400mg/kg of Alhagi maurorum alcoholic and aqueous extracts respectively orally. The period of experiment was 10 days. Blood and urine samples were collected from rabbits on the 10th day. The results recorded a significant decrease in serum creatinine, urea, uric acid and crystalluria in Cymbopogon proximus and Alhagi maurorum groups compared to sulfadimidine treated group.We conclude that Cymbopogon proximus and Alhagi maurorum have a nephroprotective and antiurolithiatic effects against sulfadimidine induced crystalluria.

scholarly journals Resistance to Fracture of Dental Roots Obturated with Different Materials

2015 ◽  
Vol 2015 ◽  
pp. 1-5 ◽  
Author(s):  
Berkan Celikten ◽  
Ceren Feriha Uzuntas ◽  
Kamran Gulsahi
Keyword(s):  
Fracture Resistance ◽  
Testing Machine ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Positive Control Group ◽  
Control Group ◽  
Root Canal Filling ◽  
Filling Materials ◽  
Group 2

The aim of this study was to compare the vertical fracture resistance of roots obturated with different root canal filling materials and sealers. Crowns of 55 extracted mandibular premolar teeth were removed to provide root lengths of 13 mm. Five roots were saved as negative control group (canals unprepared and unfilled). Fifty root canals were instrumented and then five roots were saved as positive control group (canals prepared but unfilled). The remaining 45 roots were randomly divided into three experimental groups (n=15root/group) and obturated with the following procedures: in group 1, glass ionomer-based sealer and cone (ActiV GP obturation system); in group 2, bioceramic sealer and cone (EndoSequence BC obturation system); and in group 3, roots were filled with bioceramic sealer and cone (Smartpaste bio obturation system). All specimens were tested in a universal testing machine for measuring fracture resistance. For each root, the force at the time of fracture was recorded in Newtons. The statistical analysis was performed by using Kruskal-Wallis and post hoc test. There were no significant differences between the three experimental groups. The fracture values of three experimental and negative control groups were significantly higher than the positive control group. Within the limitations of this study, all materials increased the fracture resistance of instrumented roots.

scholarly journals Comparative study of the antihypertensive effects of hexane, chloroform and methanol fractions of essential oil of Alpinia zerumbet in rats Wistar

2016 ◽  
Vol 18 (1) ◽  
pp. 113-124 ◽  
Author(s):  
G.H. CUNHA ◽  
F.V. FECHINE ◽  
F.A. FROTA BEZERRA ◽  
M.O. MORAES ◽  
E.R. SILVEIRA ◽  
...  
Keyword(s):  
Blood Pressure ◽  
Vascular Reactivity ◽  
Antihypertensive Effect ◽  
Negative Control Group ◽  
Negative Control ◽  
Control Group ◽  
Resonance Spectroscopy ◽  
Hypertensive Rats ◽  
Male Wistar Rats ◽  
Main Components

ABSTRACT The aim of this study was to characterize components of the EOAz and its hexane (HFEOAz), chloroform (CFEOAz) and methanol (MFEOAz) fractions, and its antihypertensive effect. EOAz was extracted from leaves by hydrodistillation. Aliquot was subjected to selective desorption with silica gel column and eluted with hexane, chloroform and methanol. The components of the EOAz and fractions were analyzed by gas chromatography coupled with mass spectrometry and nuclear magnetic resonance spectroscopy of hydrogen. Experiments of vascular reactivity were performed with isolated aortic rings of male Wistar rats. Antihypertensive effect was evaluated in hypertensive rats submitted to the inhibition of synthesis of nitric oxide. Blood pressure was measured indirectly by tail plethysmography. MFEOAz showed the lowest EC50 (150.45 µg/mL), 1,8-cineole (27.81%) and terpinen-4-ol (57.35%) as main components. Single administration by nasogastric tube of EOAz, fractions and captopril significantly reduced the blood pressure of hypertensive rats, when compared to animals of the negative control group with distilled water. In conclusion, the potency of the MFEOAz was higher than that of EOAz and other fractions. The antihypertensive effect of EOAz and fractions was similar, higher than the negative control and lower than that of captopril.

scholarly journals STUDY OF THE POSSIBLE ANTIHYPERTENSIVE AND HYPOLIPIDEMIC EFFECTS OF AN HERBAL MIXTURE ON L-NAME-INDUCED HYPERTENSIVE RATS

2016 ◽  
Vol 9 (5) ◽  
pp. 85 ◽  
Author(s):  
Doha Aboubaker ◽  
Souad E El-gengaihi ◽  
Mouchira Abdel Salam ◽  
Bassant Mm Ibrahim ◽  
Seham El-batran
Keyword(s):  
Blood Pressure ◽  
Aqueous Extract ◽  
Medical Condition ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Chronic Medical Condition ◽  
Control Group ◽  
Hypertensive Rats ◽  
Improve Blood Pressure Control

ABSTRACTObjective: Hypertension is a chronic medical condition. Diet can improve blood pressure control and decrease the risk of health complication.Methods: In this study, four plants: Roselle, Marjoram, Chamomile, and Doum were extracted by water. Equal portions of them were mixed. Lethaldose 50% of the mixture was assayed; the dose which did not cause any mortality was 266.94 mg/100 g body weight. Animals were classified into fivegroups: Negative control group, positive control group where hypertension was induced by L-name, two groups treated with two doses of the mixture,and a group treated with prazosin as a standard treatment. Treatment of hypertensive rats continued for 4 successive weeks.Results: Treatment with the mixture showed a significant reduction in blood pressure of hypertensive rats, as well as serum cholesterol, low-densitylipoprotein-cholesterol, and urea levels when compared to positive control group.Conclusion: The results obtained suggest that the aqueous extract is efficient as an antihypertensive and hypolipidemic agent.Keywords: Rats, Aqueous extract, Hypertension, Hyperlipidemia, L-name.

scholarly journals Correlation between International Normalized Ratio values and sufficiency of two different local hemostatic measures in anticoagulated patients

2014 ◽  
Vol 08 (04) ◽  
pp. 475-480 ◽  
Author(s):  
Mohamed Zaghlool Amer ◽  
Samah I. Mourad ◽  
Ahmed S. Salem ◽  
Ehab Abdelfadil
Keyword(s):  
Tranexamic Acid ◽  
Oral Anticoagulant Therapy ◽  
Negative Control Group ◽  
International Normalized Ratio ◽  
Acid Group ◽  
Negative Control ◽  
Control Group ◽  
Significant Difference ◽  
Group 2 ◽  
Anticoagulated Patients

ABSTRACT Objectives: The management of patients receiving oral anticoagulant therapy (OAT) undergoing minor oral surgeries is controversial. This study was designed to evaluate the correlation between International Normalized Ratio (INR) values and the sufficiency of two different local hemostatic measures in controlling postextraction bleeding in anticoagulated patients. Materials and Methods: One hundred and sixty patients receiving Warfarin OAT were included in this study. Patients were selected so that 80 patients have INR values of ≤2, whereas the remaining patients have the INR values ranging from 2 to 3. Forty patients were then randomly selected from each category to form two equal groups. Forty-five patients who had never been on OAT were selected as a negative control group (group 1). Failure to achieve hemostasis using a pressure pack was managed using either tranexamic acid (group 2) or Ankaferd Blood Stopper (ABS) (group 3). Results: The INR values of patients included in group 2 and 3 ranged from 1.5 to 3, with a mean of 2.2. No significant difference was recorded between the use of either tranexamic acid or ABS in achieving hemostasis in anticoagulated patients with INR values ranging between 2 and 3 (P = 0.93). Conclusion: Based on our findings, ABS is a hemostatic agent of good efficacy. The effect of ABS in controlling post-extraction bleeding in anticoagulated patients with INR values ≤3 is comparable to tranexamic acid with no evidence to support the superiority of tranexamic acid over ABS.

Comparison of conservative management and juvenile pubic symphysiodesis in the early treatment of canine hip dysplasia

2008 ◽  
Vol 21 (03) ◽  
pp. 267-279 ◽  
Author(s):  
G. Dravelli ◽  
L. Vezzoni ◽  
M. De Lorenzi ◽  
A. Corbari ◽  
A. Cirla ◽  
...  
Keyword(s):  
Early Treatment ◽  
Hip Dysplasia ◽  
Skeletal Maturity ◽  
Negative Control Group ◽  
Negative Control ◽  
Control Group ◽  
Homogeneous Groups ◽  
Group 2 ◽  
Canine Hip Dysplasia ◽  
Group 1

Summary Objectives: To evaluate the efficacy of juvenile pubic symphysiodesis (JPS) in a clinical setting for the early treatment of canine hip dysplasia (CHD), and to identify its indications and contraindications. Methods: The final degree of CHD using the FCI (Fédération Cynologique Internationale) CHD classification in 5 Grades (A, B, C, D, E) was assessed at skeletal maturity in two homogeneous groups of dogs assessed at the age of 14 to 22 weeks and selected according to their susceptibility to CHD; one group was treated with JPS and one group was conservatively managed. Two hundred seventeen puppies completed the study; 81 were treated with JPS (group 1) and 76 were conservatively managed (group 2). A third group of 60 puppies with normal hips was followed as a negative control group. Results: In group 1, 43.2% of the puppies had regression or a lack of progression of the disease in the final evaluation (Grade A & B), 25.9% had mild CHD (Grade C) and 30.9% had moderate and severe CHD (Grade D & E). In group 2, 23.6% of the puppies did not show any development of the disease (Grade A & B), 21.1% had mild CHD (Grade C) and 55.3% developed moderate to severe CHD (Grade D & E). Further investigation was done by comparing the severity of early signs of susceptibility to CHD with the final FCI Grades at adulthood in both groups. Clinical significance: The JPS procedure increased the odds of arresting or limiting the progression of CHD in mild to moderate grades of CHD, while it was less effective or ineffective in more severe forms.

scholarly journals Comparative Study of the Bioactivity of Two Bioceramic Materials

2018 ◽  
Vol 21 (2) ◽  
pp. 73-81
Author(s):  
Margarita Trujillo-Hernández ◽  
Rebeca E. Flores-Ventura ◽  
Angélica Suárez-Porras MSc ◽  
Leandro García-González PhD ◽  
Julián Hernández-Torres PhD ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Calcium Phosphate ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Carbonated Apatite ◽  
Human Dentine ◽  
Group 2 ◽  
Group 1

Calcium silicate-based materials have been shown to be bioactive due to their ability to produce biologically compatible carbonated apatite. The objective of this study was to analyze the bioactivity of Biodentine ™ and MTA Repair HP® in contact with human dentine discs, which were sealed and divided randomly to form four groups: group 1 Biodentine™, group 2 MTA Repair HP®, positive control group MTA Angelus® and negative control group IRM®, which were incubated in PBS solution for 10 days, for a subsequent analysis by means of MEB-EDS and Raman spectroscopy. The three calcium-based materials analyzed in this study proved to be bioactive because upon contact with a phosphate-based solution they were triggered at the onset of amorphous calcium phosphate, as the precursor during the formation of carbonated apatite.

scholarly journals ISONIAZID (INH)

2018 ◽  
Vol 25 (10) ◽  
pp. 1587-1595
Author(s):  
Umer Aleem ◽  
Rahman Shah ◽  
Noor Khan ◽  
M. Suliman
Keyword(s):  
Serum Bilirubin ◽  
Statistical Significance ◽  
Negative Control Group ◽  
Protective Role ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Group Iii ◽  
Group I ◽  
Group 2

Objectives: Hepatotoxicity is the most complicated side effect of isoniazid (inh)in the patient treated for tuberculosis. In causes 8–30% hepatotoxicity in the developing world.Metabolism of INH produces a metabolite, called acetyl isoniazid. In this study hepatoprotectiveeffect of honey, in isoniazid induced animal model was assessed. Study Design: Randomizedcontrol trial. Setting: Saidu Medical College, Saidu Sharif Swat, KP. Period: October ToDecember 2017. Material and Methods: 40 healthy male rabbits were assigned randomly tothe group i, ii, iii and iv by using lottery method. Ten animals were grouped each row. Theisoniazid-induced hepatotoxic model was created by giving 50 mg inh/kg orally on daily basisfor eleven days. Group i was taken as negative control group ii as a positive control. Group iii andiv were experimental groups treated with 50 mg /kg/day and 100 mg /kg/day buckwheat honeyrespectively for eleven days. SPSS Version 16 software was used, mean, s.d. were determinedin all the groups. Values of serum bilirubin, sgpt, and alkaline phosphatase were comparedwith each other using pairt-test. Results: SGPT, Serum bilirubin, and alkaline phosphatasewere obtained in all the animals. Comparing group 1 negative control with group 2, 3 and 4shows statistical significance, (p=0.00). Comparing group 2 positive control with 3 and 4 showsstatistical significance, (p=0.00). Further comparing group 3 with group 4 also shows statisticalsignificance (p=0.00). Conclusion: From the above finding, it has been revealed that honeyhas got a protective effect in regressing hepatitis that has been induced in rabbit’s model byhigh doses of isoniazid. Related studies performed in which different chemicals and drugs havebeen tried for their protective role in isoniazid induced hepatitis also shows a similar type ofresults.

scholarly journals PENGARUH PEMBERIAN SUSU BEKATUL TERHADAP KONSENTRASI KOLESTEROL DAN TRIGLISERIDAPADA TIKUS PUTIH WISTAR DIABETES

2019 ◽  
Vol 12 (02) ◽  
Author(s):  
Husnul Khatimah ◽  
Saifuddin Sirajuddin ◽  
Zainal Zainal
Keyword(s):  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Cholesterol Concentration ◽  
Control Group ◽  
Group 4 ◽  
White Rats ◽  
Group 3 ◽  
Group 2 ◽  
Group 1

ABSTRACT In diabetics there is usually an increase in free fatty acids in the blood and increases the concentration of cholecerols and triglycerides, this condition is called diabetes dyshidemia. This study aimed to find out at the effect of bran milk treatment on the changes of the concetration of cholesterol and triglycerides in diabetetic white wistar rats. Conducted on May 15 until 15 August 2018. Experimental research pre-post test with control design. Subjects of twenty wistar white rats were made diabetic by injecting alloxan 140mg / kg. After being declared diabetic, the rats were divided into four groups, namely group 1 (negative control), group 2 (positive control), group 3 (bran milk 0.54g / 200 gr) and group 4 (bran milk 1.08g / 200gr). The intervention was given for fourteen days. The data analysis used the paired T-test to test the subject before and afterthe treatment and the One-Way ANOVA test was used to test the difference between the groups. The research results indicated that  the treatment with bran milk there were no significant differences in cholesterol and triglyceride concentrations in the four groups. But when viewed from changes before and after the intervention showed that group 4 (bran milk 1.08g / 200gr) decreased cholesterol concentration and in group 3 (bran milk 0.54g / 200gr) a decrease in triglycerides was better than group 1 (control negative) and group 2 (positive control). It was concluded that the administration of bran milk can reduce the concentration of cholesterol and triglycerides in wistar diabetic white rats.Keywords : Bran milk, Cholesterol, Triglycerides, Rats, Diabetic

Sign in / Sign up

Export Citation Format

Share Document