Resistance of Pseudomonas Aeruginosa From Clinical and Environmental Sources to Heavy Metals in Hilla City, Iraq

2014 ◽  
Vol 2 (1) ◽  
pp. 107-115 ◽  
Author(s):  
Alaa Al-Charrakh ◽  
Raafet Al-Enzi
Keyword(s):  
Heavy Metals ◽  
Pseudomonas Aeruginosa

scholarly journals Resistance of bacteria isolated from leachate to heavy metals and the removal of Hg by Pseudomonas aeruginosa strain FZ-2 at different salinity levels in a batch biosorption system

2021 ◽  
Vol 31 (1) ◽  
Author(s):  
Muhammad Fauzul Imron ◽  
Setyo Budi Kurniawan ◽  
Siti Rozaimah Sheikh Abdullah
Keyword(s):  
Heavy Metals ◽  
Heavy Metal ◽  
Pseudomonas Aeruginosa ◽  
Fresh Water ◽  
Landfill Leachate ◽  
Saline Water ◽  
Resistant Bacteria ◽  
Sanitary Landfill ◽  
Salinity Levels ◽  
Sanitary Landfill Leachate

AbstractLeachate is produced from sanitary landfills containing various pollutants, including heavy metals. This study aimed to determine the resistance of bacteria isolated from non-active sanitary landfill leachate to various heavy metals and the effect of salinity levels on the removal of Hg by the isolated bacterium. Four dominant bacteria from approximately 33 × 1017 colony-forming units per mL identified as Vibrio damsela, Pseudomonas aeruginosa, Pseudomonas stutzeri, and Pseudomonas fluorescens were isolated from non-active sanitary landfill leachate. Heavy metal resistance test was conducted for Hg, Cd, Pb, Mg, Zn, Fe, Mn, and Cu (0–20 mg L− 1). The removal of the most toxic heavy metals by the most resistant bacteria was also determined at different salinity levels, i.e., fresh water (0‰), marginal water (10‰), brackish water (20‰), and saline water (30‰). Results showed that the growth of these bacteria is promoted by Fe, Mn, and Cu, but inhibited by Hg, Cd, Pb, Mg, and Zn. The minimum inhibitory concentration (MIC) of all the bacteria in Fe, Mn, and Cu was > 20 mg L− 1. The MIC of V. damsela was 5 mg L− 1 for Hg and >  20 mg L− 1 for Cd, Pb, Mg, and Zn. For P. aeruginosa, MIC was > 20 mg L− 1 for Cd, Pb, Mg, and Zn and 10 mg L− 1 for Hg. Meanwhile, the MIC of P. stutzeri was > 20 mg L− 1 for Pb, Mg, and Zn and 5 mg L− 1 for Hg and Cd. The MIC of P. fluorescens for Hg, Pb, Mg, and Zn was 5, 5, 15, and 20 mg L− 1, respectively, and that for Cd was > 20 mg L− 1. From the MIC results, Hg is the most toxic heavy metal. In marginal water (10‰), P. aeruginosa FZ-2 removed up to 99.7% Hg compared with that in fresh water (0‰), where it removed only 54% for 72 h. Hence, P. aeruginosa FZ-2 is the most resistant to heavy metals, and saline condition exerts a positive effect on bacteria in removing Hg.

Biosorption of Heavy Metals by Pseudomonas aeruginosa Isolated from a Petroleum Contaminated Site

2007 ◽  
Vol 20-21 ◽  
pp. 615-618 ◽  
Author(s):  
R. Maria Pérez ◽  
A. Abalos ◽  
José Manuel Gómez ◽  
Domingo Cantero
Keyword(s):  
Heavy Metals ◽  
Pseudomonas Aeruginosa ◽  
Contaminated Site ◽  
Metallic Ions ◽  
Solution Ph ◽  
Initial Concentration ◽  
Metal Biosorption ◽  
Ph Range ◽  
The Individual ◽  
A Site

The study describes the sorption of Cr, Cu, Mn and Zn by Pseudomonas aeruginosa AT18 isolated from a site contaminated with petroleum and heavy metals. The concentrations studied (mgL-1) were Cr-50, Cu-49, Mn-60 and Zn-70. The solution pH and ionic strength were very important factors in the metal biosorption performance and the biosorption capacity of Pseudomonas aeruginosa AT18 for Cr3+ Cu2+, Mn2+ and Zn2+. In aqueous solution the biosorption increased with increasing pH in the range 5.46-7.72. The results obtained in the experimental assays show that Pseudomonas aeruginosa AT18 has the capacity for biosorption of the metallic ions Cr3+, Cu2+ and Zn2+ in solutions, although its capacity for the sorption of manganese is low (22.39 mg Mn2+/g of biomass) in comparison to the Cr3+, Cu2+ and Zn2+ ions, as shown by the individual analyses. However, 20% of the manganese was removed from an initial concentration of 49.0 mgL- 1, with a Qm value similar to that obtained in solutions containing mixtures of Cr3+ Cu2+, Mn2+ and Zn2+. The chromium level sorbed by Pseudomonas aeruginosa AT18 biomass was higher than that for Cu, Mn and Zn, with 100% removal in the pH range 7.00-7.72 and a Qm of 121.90-200.00 mg of Cr3+/g of biomass. The remove of Cr, Cu and Zn are a result also of precipitation processes.

scholarly journals Biosurfactants produced by metal-resistant Pseudomonas aeruginosa isolated from Zea mays rhizosphere and compost

2020 ◽  
Vol 12 (1) ◽  
pp. 101-112
Author(s):  
José Luis Aguirre-Noyola ◽  
Yaneth Romero Ramírez ◽  
Jesus Carlos Ruvalcaba Ledezma ◽  
Angela Victoria Forero Forero ◽  
Renato León Rodríguez ◽  
...  
Keyword(s):  
Heavy Metals ◽  
Heavy Metal ◽  
Pseudomonas Aeruginosa ◽  
Antimicrobial Activity ◽  
Zea Mays ◽  
Metal Tolerance ◽  
Heavy Metal Tolerance ◽  
16S Rdna Gene ◽  
Microbial Strains ◽  
Metal Tolerant Bacteria

Contextualization: Pseudomonas aeruginosa is capable of producing biosurfactants which have many uses in bioremediation and the production of antiviral, antibacterial, antiparasitic, sporicidal and antifungal agents, among others.   Knowledge gap: This study describes the production of mono and di-rhamnolipid biosurfactants by P. aeruginosa strains isolated from Zea mays rhizosphere and composts in the state of Guerrero, Mexico.  Purpose: The overall aims were to investigate biosurfactant, pyocyanin production, and tolerance to heavy metals and antimicrobial activity capacity than biosurfactants produced from P. aeruginosa strains from corn rhizosphere and compost in Mexico. Methodology: Biosurfactant production was determined based hemolysis on blood agar, blue halos in CTAB-Methylene blue agar, drop collapse test and production of foam on PPGAS broth, the emulsion index (IE24) and antibacterial capacity. The strains were identified by sequence of the 16S rDNA gene and their resistance to heavy metals were also evaluated. Results and conclusions: Two strains isolated from Zea mays rhizosphere (PAM8, PAM9) were the best biosurfactant producers and their extracts showed antimicrobial activity against Grampositive and Gramnegative bacteria. PAM8 and PAM9 showed >30% of cellular hydrophobicity to hydrocarbons, and were capable of emulsifying toluene, cyclohexane, petroleum, diesel and oils. All strains showed the same profile of heavy metal tolerance (As5+ >As3+ >Zn2+ >Pb2+ >Fe3+ >Cd2+ >Cu2+ >Cr6+ in concentrations of 20, 10, 10, 6, 4, 4, 2 and 2 mM., respectively). The isolation of biosurfactant-producing and heavy-metal tolerant bacteria from Zea mays rhizosphere and compost in Guerrero demonstrates the capacity for this region to harbor potentially important microbial strains for industrial or bioremediation applications.

Effects of heavy metals on Pseudomonas aeruginosa and Bacillus thuringiensis

1998 ◽  
Vol 65 (1-2) ◽  
pp. 73-82 ◽  
Author(s):  
A. Hassen ◽  
N. Saidi ◽  
M. Cherif ◽  
A. Boudabous
Keyword(s):  
Heavy Metals ◽  
Pseudomonas Aeruginosa ◽  
Bacillus Thuringiensis

scholarly journals Cadmium and lead tolerant bacteria isolated from industrial waste water

2017 ◽  
Vol 26 (1) ◽  
pp. 29-38
Author(s):  
Md Arifur Rahman Bhuiyan ◽  
Tazeen Fatima Khan ◽  
Shahjahan Choudhury ◽  
SM Imamul Huq
Keyword(s):  
Waste Water ◽  
Heavy Metals ◽  
Pseudomonas Aeruginosa ◽  
Bacillus Cereus ◽  
Industrial Waste ◽  
Resistant Bacteria ◽  
Bacterial Isolates ◽  
Industrial Waste Water ◽  
Acinetobacter Baumanii ◽  
Cadmium And Lead

An in vitro study was conducted to isolate, identify and characterize heavy metal resistant bacteria from industrial waste water and to determine their tolerance capacity to cadmium and lead. Different morphological, physiological and biochemical tests were carried out to identify the bacterial isolates in the waste water. A total of eight bacterial isolates viz., Staphyllococcus intermedius, Pseudomonas aeruginosa, Bacillus cereus, Bacillus subtilis, Escherichia coli, Acinetobacter baumanii, Pseudomonas flavescens and Acinetobacter lwofii were identified from metal polluted tannery and steel industrial areas. Bacterial response to cadmium tolerance was determined by treating them with CdCl2 solution at a rate of 1, 2, 2.5 and 5 μg/ml. In case of lead tolerant bacteria, PbCl2 solution was applied at a rate of 0.05, 0.125, 0.2 and 0.5 μg/ml. Pseudomonas aeruginosa isolated from waste water of steel industries and Bacillus cereus from tannery area was found to be the most tolerant species to the different doses of cadmium and lead. The study indicated that Pseudomonas aeruginosa and Bacillus cereus could be good candidates for the treatment and elimination of heavy metals from industrial waste water. The present study may be helpful to the bioremediation of heavy metals in the contaminated environment. Dhaka Univ. J. Biol. Sci. 26(1): 29-38, 2017 (January)

scholarly journals Bioaccumulation of Heavy Metals using Selected Organisms Isolated from Electronic Waste Dumpsite of two South-Western States in Nigeria

2017 ◽  
pp. 29-40
Author(s):  
Akintokun K. Aderonke ◽  
Onatunde O. Oladimeji ◽  
Olufunke B. Shittu ◽  
Itunu C. Okeyode ◽  
Michael O. Taiwo
Keyword(s):  
Heavy Metals ◽  
Pseudomonas Aeruginosa ◽  
Aspergillus Niger ◽  
Bacillus Licheniformis ◽  
Metal Binding ◽  
Contaminated Soils ◽  
Human Life ◽  
Electronic Waste ◽  
Bacteria And Fungi ◽  
Micrococcus Roseus

Heavy metals from electronic wastes can accumulate to alarming concentrations in soils, causing significant detrimental impacts on human life and the environment. Bioaccumulation of heavy metals by bacteria and fungi has been a major focus of most bioremediation studies owing to the excellent metal-binding properties. The current study was conducted to isolate the most promising Zn, Cu and Pb tolerant microorganisms from contaminated soils, and to assess their metal accumulating abilities. Bacillus licheniformis, B. polymyxa, Pseudomonas aeruginosa, Micrococcus roseus, Aspergillus niger and A. flavus were selected for the bioaccumulation study, based on their known tolerance to heavy metals. Bacillus licheniformis was most efficient in the removal of Cu (71.3 %) and Pb (70.1 %). Pb accumulation for Aspergillus flavus was 65.76 %. Zn accumulation for Pseudomonas aeruginosa and Aspergillus niger were 74.1 % and 78.3 %, respectively. This study concluded that all these microorganisms have potential for bioremediating soil environments contaminated with heavy metals.

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